• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.05a
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• pp.27-35
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• 2003

(시험 1) 육용종계 산란기의 단백질공급수준에 관한 연구: 육용종계 산란기의 적정 단백질공급수중을 구명하기 위하여 강원도 홍천군 북방면소재 홍천종계에서 케이지 사양형태의 아바에이카 육용종계 400수를 공시하여 2001년 2월부터 2001년 12월까지 40주간 (24∼64주령) 1일1수당 단백질 공급량을 18, 20, 22, 24g으로 달리하는 4개처리로 사양시험을 실시한 결과를 요약하면 다음과 같다. 1) 총 산란율과 종란 산란율은 모두 처리간에 유의적인 차이가 있었으며 (P<0.05), 20g 공급구가 가장 높았으나 18∼22g 공급구간에는 유의차가 없어고 24g 공급구는 20g 공급구에 비해 유의적으로 낮았다(P<0.05). 2) 평균난중은 1일 단백질공급수준이 증가할수록 증가하는 경향이었으나 (P<0.05), 20∼24g 공급구간에는 유의차가 없었고 18g 공급구만 24g 공급구와 유의적인 차이가 있었다. 성계생존율은 처리간에 유의적인 차이가 없었다. 3) 계란kg당 또는 계란1개당 사료요구율과 ME요구량은 모두 1일 CP 20g 공급구가 가장 우수했으며(P<0.05), 계란 kg당 사료요구율과 ME요구량에서는 CP 18g 공급구계란1개당 사료요구율과 ME요구량에서는 CP 24g 공급구만 CP 20g공급구오 유의차를 보였다(P<0.05). 4) 계란kg당 또는 계란 1개당 CP요구량은 모두 1일 단백질 공급량이 많아질수록 증가하였으나(P<0.05), 18g과 20g 공급구간에는 유의차가 없었다. 5) 계란kg당 또는 계란 1개당 사료비는 모두 CP 20g 공급구가 가장 우수하였으나(P<0.05), 계란1개당 사료비에서는 18∼20g 공급구간에 유의차가 없었다. (시험 2) 육용종계 하절기 산란피크계의 에너지 공급수준에 관한 연구: 육용종계 산란기의 적정 에너지공급체계를 구명하기 위하여 강원도 홍천군 북방면소재 홍천종계에서 케이지 사양형태의 로스 육용종계 400수를 공시하여 2002년 4월부터 2003년 1월까지 40주간 (24∼64주령) 표2와 같은 4가지 에너지공급체계로 사양시험을 실시한 결과를 요약하면 다음과 같다. 1) 총 산란율, 종란 산란율, 평균난중 및 성계 생존율은 모두 처리간에 유의적인 차이가 인정되지 않았으나, 산란율은 1일 에너지공급량이 많을수록 오히려 저하하는 경향을 보였다. 2) 사료요구율, ME 및 CP요구량과 사료비는 모두 1일 에너지공급량이 많아질수록 증가하는 경향을 보였다(P<0.05).

• Journal of Environmental Health Sciences
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• v.14 no.1
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• pp.103-113
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• 1988

This experiment was performed to study the sequential accumulation of mercury in selected tissues of gold fish (Carassius auratus) exposed to 2, 6, 30, 120 and 300 $\mu$g Hg/1 as HgCl$_2$. In order to prepare treatment groups suitable for the present study, one control and five experimental groups, which were composed of I (2 $\mu$g/l), II (6$\mu$g/l), III (30$\mu$g/l), IV (120$\mu$g/l), V (300 $\mu$g/l), were used in 180 liter glass aquaria. The experiment was started by transfering 20 fish of average total length 140 $\pm$ 20 mm to each of the six tanks and allowing the uptake to take place for 12 weeks period. Fish were killed after time periods of 1, 2, 4, 8, and 12 weeks, and samples were disected by five parts gill, kidney liver, muscle and egg. The summarized results were as follows: 1. In control group, low concentrations of mercury(range 0.01-0.11 $\mu$g/g)were determined in the all selected tissues. 2. In experimental group, the average levels of mercury residues in the gill, kidney, liver muscle and egg were 3.61-189.54 $\mu$g/g, 13.91-182.58 $\mu$g/g, 8.56-66.49 $\mu$g/g, 0.30-20.33 $\mu$g/g, and 1.63-23.76$\mu$g/g, respectively. 3. The mercury residues in selected tissues of the experimental group were generally 230-9100 times higher than those of the control group. 4. The amounts of methylmercury per total mercury in the muscle after 12 weeks were 0.10/0.30 $\mu$g/g(33.33%) in the I group, 0.14/1.18$\mu$g/g(11.86%) in the II group, 0.25/5.76 $\mu$g/g(4.34%) in the III group, 0.39/11.48$\mu$g/g(3.40%) in the IV group and 0.40/20.33 $\mu$g/g(1.97%) in the V group.

• Korean Journal of Food Science and Technology
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• v.42 no.2
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• pp.246-249
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• 2010

Diacylglycerol (DAG) was produced from lipase by the catalyzed synthesis of soybean oil (SBO) and glyceryl monooleate (GMO) with Lipozyme TLIM (Thermomyces lanuginosa). Effects of reaction time, molar ratio and enzyme road were studied. When 2:1, 1:1 and 1:2 (SBO:GMO) molar ratios with 20% Lipozyme TLIM were applied in a 1-hr reaction, the concentrations of DAG produced were 17.8, 20.0 and 20.4 g/100 g oil, respectively. Different amounts (2, 5, 10 and 20%) of Lipozyme TLIM were used at a 1:2 (SBO:GMO) molar ratio, and the concentrations of DAG produced in a 1-hr reaction were 10.8, 14.0, 16.9 and 20.4 g/100 g oil, respectively. During a 72-hr reaction, 10.8-22.7 g/100 g oil of DAG were produced under the reaction conditions in this study.

• Food Science of Animal Resources
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• v.28 no.1
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• pp.21-26
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• 2008

This study was carried out to evaluate the nutritional properties of spreadable liver product. The composition of fresh hog liver used as raw material was 70.5% moisture, 5.2% fat, 20.3% protein and 1.2% ash. The composition of the spreadable liver product was 55.3% moisture, 19.4% protein, 21.6% fat and 3.7% ash. The total amino acid content of fresh liver was 18.69 g/100 g with glutamic acid at the highest level of 2.57 g/100 g. The total amino acid content of liver product was 18.03 g/100 g with glutamic acid at 2.28 g/100 g. The essential amino acid content was found to be 42% of total amino acids. The fatty acid analysis of fresh liver revealed oleic acid to be present at the highest level of 28% in the unsaturated fatty acid portion, and palmitic acid to be highest at 20.7% in the saturated fatty acid portion. The oleic acid content of the liver product was 40%, and the palmitic acid content was 20.4%. The level of cholesterol in fresh liver was 178.0 mg/100 g compared to 118.0 mg/100 g in the liver product. Regarding mineral analysis, the K and P contents of fresh liver were 362.2 mg/100 g and 339.1 mg/100 g, respectively, and 336.1 mg/100 g and 213.3 mg/100 g in the liver product, respectively. Many other minerals including Na, Mg, Ca and Fe were present in the product. Based on these results, the spreadable liver product made with hog liver was found to be a quality food with nutritional benefits and that is easy to consume.

• Korean Journal of Animal Reproduction
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• v.16 no.3
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• pp.269-276
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• 1992

Studies were carried out to find the freezing media which gives no ice crystals in single(glycerol, ethylene glycol, dimethyl sulfoxide(DMSO)) and mixture solutions(glycerol＋propylene glycol, glycerol＋ethylene glycol) of permeable cryoprotectants in vitrification solution and to study effects of VS on the survival of vitrified mouse morulae. The results are summarized as follows: 1. In toxicity test of permeable cryoprotectants, 30% glycerol of single solution showed the highest FDA-score(4.1) in mouse morulae frozen compared among other single solutions. The FDA-score(4.1) of 30% glycerol was higher than 30% ethylene glycol(3.6) and DMSO(1.4( (P<0.05). 2. 20, 30 or 40% single solution of permeable cryoprotectants containing m-PBS with 10% sucrose and 20% BSA was not crystallized during cooling, but crystallized during warming. However, the 30% mixture solution of the two permeable cryoprotectants was not crystallized both during cooling and warming.3. When mouse morulae were frozen in 30% mixture solutions of two permeable cryoprotectants(glycerol and propylene glycol, glycerol and ethylene glycol), highest FDA-score(4.5) was obtained in a mixture solution of 20% glycerol and 10% ethylene glycol(20G10E) than other 30% mixture solutions(10G20E, 15G15E, 20G10P, 15G15P, 10G20P) and there was significant difference between 20G10E and 10G20E(P<0.05).

• Journal of Life Science
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• v.18 no.4
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• pp.494-502
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• 2008

The anaphylaxis shock reaction on the whole cells of H. pylori exhibited a symptom of slight illness for the first and second medication of causing antigen at an antigen concentration of WC (H) $60\;{\mu}g/100\;{\mu}l$ for WC (H) and no anaphylaxis shock symptom was observed at an antigen concentration of $20\;{\mu}g/100\;{\mu}l$ for WC (L). In the case of anaphylaxis shock reaction on the crude urease, no symptom was observed at an antigen concentration of $20\;{\mu}g/100\;{\mu}l$ for both urease (L) and urease (H). In the heterologous passive cutaneous anaphylaxis (PCA) test using a guinea pig-rat, no positive reaction was detected in all the medication groups of WC (H), WC (L), urease (H) and urease (L). In the skin sensitization test, it was observed that the best antigen concentration not causing skin disorder at each of $80\;{\mu}g/100\;{\mu}l$, $40\;{\mu}g/100\;{\mu}l$, $20\;{\mu}g/100\;{\mu}l$, and $20\;{\mu}g/100\;{\mu}l$ was $40\;{\mu}g/100\;{\mu}l$.

• Journal of environmental and Sanitary engineering
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• v.5 no.2
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• pp.53-62
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• 1990

There has been considerable organisms the most indicative of the sanitary quality of food products. Of the suggested indices of sanitary quality of foods are coliform organism and SPC. In addition to the usual index organisms on fish products it is also necessary to determining the sanitary quality The authors have tested with 282 fish products (spring : 39, summer : 109, autumn : 112, winter . 22) 1. The range of microbial organism in fish products are as follows Spring : SPC is $13{\times}10^3\; to\; 50{\times}10^8/g$, coliform group is $16{\times}10^2\; to\; 48{\times}10^8/g$ and 2. coli is 50 to $22{\times}10^4/100g$. Summer : SPC is 70 to$64{\times}10^9/g$. coliform group is 25 to $26{\times}10^8/g$ and E. coli is 20 to $22{\times}10^4/100g$. Autumn : SPC is $10{\times}10^3\; to\; 46{\times}10^8/g$, coliform group is 200 to $20{\times}10^5/g$ and E. coli is 20 to $22{\times}10^4/100g$. Winter : SPC is $30{\times}10^3\; to\;30{\times}10^8/g$. coliform group is $21{\times}10^2\;to\;16{\times}10^3/g$ and E. coli is 20 to 790/100g. Salmonella and Staphylococcus species were not in 282 fish products.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.1
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• pp.16-22
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• 2013

Water is the most important resource for the maximum water use efficiency and yield of maize. Water has to be applied moderately based on the water requirement of maize. Crop water requirement (WR) is a function of the potential evapo-transpiration (PET) and crop coefficient (Kc). PET can be estimated by the climate data measured at the weather station in the production region. Kc was measured by the NIAST (RDA) through lysimeter experiments. In this study, the growth stage of maize was divided into five ones (G-1: Apr. 25 ~ May 20, G-2: May 21 ~ Jun. 20, G-3: Jun. 21 ~ Jul. 20, G4: Jul. 11 ~ Jul. 25, G5: Jul. 26 ~ Aug. 20). The average PET during maize growing season of the 45 areas was 2.85 mm $day^{-1}$. The highest water requirement was at the G-3 stage among the maize growth stages. The mean water requirement (MWR) according to growth stage was 1.74 ~ 2.42 (average 2.02), 2.99 ~ 4.21 (average 3.41), 3.82 ~ 5.25 (average 4.41), 3.05 ~ 4.31 (average 3.48), and 2.62 ~ 3.49 (average 3.01) mm $day^{-1}$ in the G-1, G-2, G-3, G-4 and G-5 stage, respectively. The total water requirement (TWR) according to growth stage was 45.37 ~ 63.04 (average 52.56), 92.54 ~ 130.59 (average 105.77), 76.46 ~ 105.09 (average 88.14), 45.73 ~ 64.67 (average 52.20), and 68.25 ~ 90.75 (average 78.33) mm in the G-1, G-2, G-3, G-4 and G-5 stage, respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.1
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• pp.31-37
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• 2010

Two field experiments were carried out in 2002 to evaluate effects of the sizes of tubers produced through a wick-based hydroponics on growth and yield of seed potatoes (Solanum tuberosum L. 'Dejima'). Nine sizes of seed tubers (<1, 1~3, 3~5, 5~7, 7~10, 10~20, 20~30, 30~50, and 50~80 g) were used in spring crop. In fall crop, however, tubers except for the 1 g and 50~80 g wereused with tubers 7 and 15 g produced through aeroponics. Emergence rate greatly increased as tuber size was increased up to 10~20 g and then leveled off in both croppings. The number of days from planting to emergence in spring crop was about 34 days regardless of tuber size, while that in fall crop decreased from 34 to 16 days as tuber size increased from 1~3 to 30~50 g. The number of stems per plant, stem length and diameter, and leaf size tended to increase with tuber size. As the seed tuber size increased up to 10~20 g, the number of tubers per plant, average tuber weight, total and seed tuber yields linearly increased and then slowly increased with seed tuber size. Total and seed potato yields were not significantly different between tubers produced through a wick-based hydroponics and an aeroponics when similar sized tubers were planted in the fall cropping.

• Environmental Analysis Health and Toxicology
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• v.23 no.3
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• pp.221-230
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• 2008

Chlorpyrifos (CPF) is a widely used organophosphate insecticide. Japanese medaka, Oryzias latipes was selected to investigate the effects of chlorpyrifos on survival rate, hatching rate, reproductivity and histopathological changes. Adult fish were exposed to CPF at concentrations of 20, 40, 80 and $160{\mu}g/L$, and the test duration was 4 weeks. An experiment with eggs was also performed under CPF exposure continued for 7 days covering from the fecundation to end-hatching stages. Survival rate of the adult medaka decreased in CPF $80{\mu}g/L$ and $160{\mu}g/L$ treatment groups compared with control, solvent control, $20{\mu}g/L$ or $40{\mu}g/L$L treatment group. Spontaneous start of feeding significantly decreased compared with the control (p < 0.01) when exposed to CPF at above 40 $\mu g/L$. Total spawning decreased by the 20 or $40{\mu}g/L$ CPF treatment compared with the control or solvent control in the test with eggs obtained from untreated adult medaka, and abnormal eggs increased in those CPF-treated groups. There were certain adverse effects at above $40{\mu}g/L$ determined from liver and gonad histological examinations. The results indicate that CPF has insignificant toxic effects in Japanese medaka at less than $20{\mu}g/L$ in long term exposure.