• Title/Summary/Keyword: G(E)factor

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Ginsenoside Rg3 suppresses mast cell-mediated allergic inflammation via mitogen-activated protein kinase signaling pathway

  • Kee, Ji-Ye;Hong, Seung-Heon
    • Journal of Ginseng Research
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    • v.43 no.2
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    • pp.282-290
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    • 2019
  • Background: Ginsenoside Rg3 (G-Rg3) is the major bioactive ingredient of Panax ginseng and has many pharmacological effects, including antiadipogenic, antiviral, and anticancer effects. However, the effect of G-Rg3 on mast cell-mediated allergic inflammation has not been investigated. Method: The antiallergic effects of G-Rg3 on allergic inflammation were evaluated using the human and rat mast cell lines HMC-1 and RBL-2H3. Antiallergic effects of G-Rg3 were detected by measuring cyclic adenosine monophosphate (cAMP), detecting calcium influx, and using real-time reverse transcription polymerase chain reaction, enzyme-linked immunosorbent assay, Western blotting, and in vivo experiments. Results: G-Rg3 decreased histamine release from activated mast cells by enhancing cAMP levels and calcium influx. Proinflammatory cytokine production was suppressed by G-Rg3 treatment via regulation of the mitogen-activated protein kinases/nuclear factor-kappa B and receptor-interacting protein kinase 2 (RIP2)/caspase-1 signaling pathway in mast cells. Moreover, G-Rg3 protected mice against the IgE-mediated passive cutaneous anaphylaxis reaction and compound 48/80-induced anaphylactic shock. Conclusion: G-Rg3 may serve as an alternative therapeutic agent for improving allergic inflammatory disorders.

Four active monomers from Moutan Cortex exert inhibitory effects against oxidative stress by activating Nrf2/Keap1 signaling pathway

  • Zhang, Baoshun;Yu, Deqing;Luo, Nanxuan;Yang, Changqing;Zhu, Yurong
    • The Korean Journal of Physiology and Pharmacology
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    • v.24 no.5
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    • pp.373-384
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    • 2020
  • Paeonol, quercetin, β-sitosterol, and gallic acid extracted from Moutan Cortex had been reported to possess anti-oxidative, anti-inflammatory, and anti-tumor activities. This work aimed to illustrate the potential anti-oxidative mechanism of monomers in human liver hepatocellular carcinoma (HepG2) cells-induced by hydrogen peroxide (H2O2) and to evaluate whether the hepatoprotective effect of monomers was independence or synergy in mice stimulated by carbon tetrachloride (CCl4). Monomers protected against oxidative stress in HepG2 cells in a dose-response manner by inhibiting the generation of reactive oxygen species, increasing total antioxidant capacity, catalase and superoxide dismutase (SOD) activities, and activating the antioxidative pathway of nuclear factor E2-related factor 2/Kelch-like ECH-associated protein 1 (Nrf2/Keap1) signaling pathway. We found that the in vitro antioxidant capacities of paeonol and quercetin were better than those of β-sitosterol and gallic acid. Furthermore, paeonol apparently diminished the levels of alanine transaminase and aspartate aminotransferase, augmented the contents of glutathione and SOD, promoted the expressions of Nrf2 and heme oxygenase-1 proteins in mice stimulated by CCl4. In HepG2 cells, paeonol, quercetin, β-sitosterol, and gallic acid play a defensive role against H2O2-induced oxidative stress through activating Nrf2/Keap1 pathway, indicating that these monomers have anti-oxidative properties. Totally, paeonol and quercetin exerted anti-oxidative and hepatoprotective effects, which is independent rather than synergy.

The Effects of Ganoderma Iucidum Extracts and Filtrate of Escherichia coli Culture on Leukocyte Chemotaxis (영지추출물(靈芝抽出物) 및 Escherichia coli 배양액(培養液)이 백혈구(白血球)의 Chemotaxis에 미치는 영향(影響))

  • Lee, Mi-Sook;Chung, Kyu-Sun
    • The Korean Journal of Mycology
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    • v.15 no.1
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    • pp.1-8
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    • 1987
  • The purpose of this experiment is to examine if G. lucidum extracts could have an effect on leukocyte migration and to select the best chemotactic factor for leukocyte migration. When chemotactic factors such as TC-199 medium, filtrate of E. coli culture, N-fMLP, and G. lucidum extracts were used, N-fMLP, G. lucidum extracts and filtrate of E. coli were positive effect, but TC-199 medium was less than others. The various concentrations of the chemotactic factors such as G. lucidum extracts(1.00%, 0.10% and 0.01%) were stimulated for the leukocytes migration in the modified Boyden chambers, however, the lowest concentration such as 0.01% was more effective than others for the chemoattractant of the leukocyte migration When the leukocyte was treated with G. lucidum extracts(1.00%, 0.10%, and 0.01%) at room temperature for 120 minutes, the chemotactic activities had a good effect at 0.01%. Therefore, leukocyte was affected by G. lucidum extracts.

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An Experimental Study on Tube-Side Heat Transfer Coefficients and Friction Factors of the Enhanced Tubes Used in Regenerators of Absorption Chillers (흡수식 냉동기의 재생기에 사용되는 전열촉진관의 관 내측 열전달계수 및 마찰계수에 대한 실험적 연구)

  • Kim, Nea-Hyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.3
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    • pp.716-723
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    • 2016
  • Enhanced tubes are used widely in the heat exchangers of absorption chillers. In regenerators, corrugated, ribbed or floral tubes are commonly used. In this study, the tube-side heat transfer coefficients and friction factors of enhanced tubes were obtained experimentally using the Wilson Plot method. The results showed that the heat transfer coefficients and the friction factors were the largest for the corrugated tube, followed by the ribbed tube. The heat transfer coefficients and friction factors of the floral tube matched those of the smooth tube within 4%, which suggests that the heat transfer and friction characteristics of the floral tube may be accounted for properly by the hydraulic diameter. The B(e+) and g(e+) were obtained from the experimental data of the corrugated and ribbed tube. The B(e+) and g(e+) of the corrugated tube matched those of the existing correlation within 20%. The present results may be used for an assessment of the heat transfer and friction characteristics of the enhanced tubes for regenerators.

Effect of Continuous Exposure to Reactive Oxygen Species on ${\gamma}$-Glutamyltranspeptidase Expression and Activity in HepG2 Cells (HepG2 세포에서 지속적인 활성 산소 노출이 ${\gamma}$-Glutamyltranspeptidase 발현과 활성에 미치는 영향)

  • Kim, Young-Whan;Choe, Dal-Ung
    • Journal of Environmental Health Sciences
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    • v.30 no.3
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    • pp.230-238
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    • 2004
  • The adverse health effects of a number of environment pollutions are related to the formation of free radicals. Induction of antioxidant defensive system in the response to an oxidative attack is an essential element of the cell to survive. CYP2E1 is easily induced by organic solvents and induces continuous formation of reactive oxygen species (ROS). ${\gamma}$-Glutamyltranspeptidase (${\gamma}$GT) plays an important role in glutathione metabolism and xenobiotic detoxification. To evaluate the characteristic of oxidative stress which induces GGT expression and to understand human antioxidant defensive response against oxidative stress induced by CYP2E1, we studied regulation of ${\gamma}$GT enzyme expression in response to various oxidative stresses in human HepG2 cells. The ${\gamma}$GT activity was not modified after exposure of acute oxidative stress inducing agents (ferric nitrilotriacetate, cumene hydroperoxide, ADP-Fe, O-tetradecanoylphorbol-13-acetate, tumor necrosis factor-alpha). To induce continuous exposure of cells to ROS, HepG2 cells were transfected by human CYP2E1 gene transiently. The CYP2E1 activity was verified with chlorzoxazone hydroxylation. Transfection of CYP2E1 showed continuous 60% increase in intracellular ROS and 240 % increase in microsomal ROS. CYP2E1 overexpressing cells showed increased ${\gamma}$GT activity (2.5-fold). The observed enhancement of ${\gamma}$GT activity correlated with a significant increase of ${\gamma}$GT mRNA (2.1-fold). Treatment with antioxidant strongly prevented the increase in ${\gamma}$GT activity. The CYP2E1 overexpression did not modify toxicity index and increased glutathione levels. These results show that continuous exposure of cells to ROS produced by CYP2E1 up-regulates ${\gamma}$GT; This may be one of the adaptive antioxidant responses of cells to oxidative insult. Present study also suggests that the induction of ${\gamma}$GT could be used as a marker of oxidative stress induced by exposure to organic solvents.

Sensitivity Analysis of Core Neutronic Parameters in Electron Accelerator-driven Subcritical Advanced Liquid Metal Reactor

  • Ebrahimkhani, Marziye;Hassanzadeh, Mostafa;Feghhi, Sayed Amier Hossian;Masti, Darush
    • Nuclear Engineering and Technology
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    • v.48 no.1
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    • pp.55-63
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    • 2016
  • Calculation of the core neutronic parameters is one of the key components in all nuclear reactors. In this research, the energy spectrum and spatial distribution of the neutron flux in a uranium target have been calculated. In addition, sensitivity of the core neutronic parameters in accelerator-driven subcritical advanced liquid metal reactors, such as electron beam energy ($E_e$) and source multiplication coefficient ($k_s$), has been investigated. A Monte Carlo code (MCNPX_2.6) has been used to calculate neutronic parameters such as effective multiplication coefficient ($k_{eff}$), net neutron multiplication (M), neutron yield ($Y_{n/e}$), energy constant gain ($G_0$), energy gain (G), importance of neutron source (${\varphi}^*$), axial and radial distributions of neutron flux, and power peaking factor ($P_{max}/P_{ave}$) in two axial and radial directions of the reactor core for four fuel loading patterns. According to the results, safety margin and accelerator current ($I_e$) have been decreased in the highest case of $k_s$, but G and ${\varphi}^*$ have increased by 88.9% and 21.6%, respectively. In addition, for LP1 loading pattern, with increasing $E_e$ from 100 MeV up to 1 GeV, $Y_{n/e}$ and G improved by 91.09% and 10.21%, and $I_e$ and $P_{acc}$ decreased by 91.05% and 10.57%, respectively. The results indicate that placement of the Np-Pu assemblies on the periphery allows for a consistent $k_{eff}$ because the Np-Pu assemblies experience less burn-up.

Effect of the Evaporating Extract from Liquor of Fermentation Using Mycelium of Phellinus linteus on the Expression of Inflammatory Proteins and the Generation of Reactive Oxygen Species (상황버섯 균사체를 이용한 발효주의 추출물이 염증관련 단백질의 발현과 세포내 활성산소 생성에 미치는 영향)

  • Lee Jun Hyuk;Choi Yung Hyun;Lee Yong Tae;Choi Sung Hee;Chung Kyung Tae;Jeong Young Kee;Choi Byung Tae
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.18 no.6
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    • pp.1825-1831
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    • 2004
  • Phellinus linteus is known as a medicinal mushroom, which has the pharmaceutical activity on tumors and inflammatory diseases in traditional Oriental medicine. However, despite extensive pharmacological studies on P. linteus, the liquor of fermentation using mycelium of P. linteus(LFMP) has not been investigated. In the present study, it was examined the effect of the evaporating extract from LFMP(E-LFMP) on the expression of inflammatory proteins and the generation of reactive oxygen species in human hepatocarcinoma HepG2 cells. E-LFMP inhibited acetaldehyde-induced morphological change in HepG2 cells. Also, E-LFMP inhibits expression of inflammatory proteins including cyclooxygenase(COX)-1 and COX-2 through suppressing nuclear translocation of nuclear factor κB(NF-κB) and degradation of inhibitory κBα(IκBα). In addition, E-LFMP inhibits generation of reactive oxygen species(ROS) by hydrogen peroxide(H₂O₂) in HepG2 cells. These results suggest that LFMP has the pharmaceutical, especially anti-inflammatory, activity similar to P. linteus mushroom.

Effect of a c-MYC Gene Polymorphism (g.3350G>C) on Meat Quality Traits in Berkshire

  • Oh, J.D.;Kim, E.S.;Lee, H.K.;Song, K.D.
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.11
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    • pp.1545-1550
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    • 2015
  • c-MYC (v-myelocytomatosis viral oncogene homologue) is a transcription factor that plays important role in many biological process including cell growth and differentiation, such as myogenesis and adipogenesis. In this study, we aimed to detect MYC gene polymorphisms, their genotype frequencies and to determine associations between these polymorphisms and meat quality traits in Berkshire pigs. We identified a single nucleotide polymorphism (SNP) in intron 2 of MYC gene by Sanger sequencing, i.e., g.3350G>C (rs321898326), that is only found in Berkshire pigs, but not in other breeds including Duroc, Landrace, and Yorkshire pigs that were used in this study. Genotypes of total 378 Berkshire pigs (138 sows and 240 boars) were determined using Hha I restriction enzyme digestion after polymerase chain reaction. Observed allele frequencies of GG, GC, and CC genotypes were 0.399, 0.508, and 0.093 respectively. Statistical analysis indicated that the g.3350G>C polymorphism was significantly associated with $pH_{45min}$ and cooking loss (p<0.05), suggesting that g.3350G>C SNP can be used for pre-selection of $pH_{45min}$ and cooking loss traits in Berkshire pigs.

A Novel Boost PFC Converter Employing ZVS Based Compound Active Clamping Technique with EMI Filter

  • Mohan, P. Ram;Kumar, M. Vijaya;Reddy, O.V. Raghava
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.8 no.1
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    • pp.85-91
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    • 2008
  • A Boost Power Factor Correction (PFC) Converter employing Zero Voltage Switching (ZVS) based Compound Active Clamping (CAC) technique is presented in this paper. An Electro Magnetic Interference (EMI) Filer is connected at the line side of the proposed converter to suppress Electro Magnetic Interference. The proposed converter can effectively reduce the losses caused by diode reverse recovery. Both the main switch and the auxiliary switch can achieve soft switching i.e. ZVS under certain condition. The parasitic oscillation caused by the parasitic capacitance of the boost diode is eliminated. The voltage on the main switch, the auxiliary switch and the boost diode are clamped. The principle of operation, design and simulation results are presented here. A prototype of the proposed converter is built and tested for low input voltage i.e. 15V AC supply and the experimental results are obtained. The power factor at the line side of the converter and the converter efficiency are improved using the proposed technique.

A Study of the Attitudes of Psychiatric Registred Nurses towards Mental illness and Mental Patients (정신과 근무 간호원의 정신질환 및 정신질환자에 대한 태도 연구)

  • 김향미
    • Journal of Korean Academy of Nursing
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    • v.12 no.1
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    • pp.13-24
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    • 1982
  • The main purpose of this study was to investigate attitudes toward mental illness and the mentally ill of psychiatric nurses in Korea and to identify the relationships between the nurses' attitudes and demographic variables. Subjects in this study were 122 psychiatricnurses and 111non-psychiatric nurses in active service at 12 hospitals in Seoul. The insrtument used for measuring attitudes was a questionaire developed by Cohen and Struening (1962) referred to as the“Opinions about Mental illness(OMI) Scale”made up to 51 Likert-type items. The findings of this study indicated hatnurses showed negative attitudetoward mental illness and the mentally ill: Very high on social restrictiveness (factor D), low on Mental Health Ideology (factor C), and Interpersonal Etiology (factor E). Since the high score on Factor A, B and the low score on Factor B, C, E reflect a negative altitudes toward mental illness, this study population related extremely negative attitudes compared to any other surveys. And of the demographic variables that related to their attitudes the education degree and the kind of the hospital in active service showed very significant differences. On the basis of the investigation the findings indicated the followings; 1) Althohg attitudes toward mental illness and the mentally ill of psychiatric nurses were not significant differences from non-psychatric nurses, there was a trend that attitudes of psychaitric nurses did show more negative responses rather than those of non-psychiatric nurses. 2) Demographic variables that relate to their attitudes on the OMI scale were the education degree of the respondent and the kind of their hospital in active service. 3) About attitudes toward mental illness and the mentally ill baccalaureate nursesshowed more positive attitudes in Factor A (Authoritarianism), Factor C (Mental Health Ideology) and Factor D (Social Restrictiveness) than diploma program murses. 4) Nurses in active Service in the private hospital revealed more positive attitudes(A,B,C,D) except Factor (E) than those in the national or public hospital. 5) The ages, duration of work, wanted or unwantedG roup of psychiatric ward and satisfactory level of psychiatric nursing service were non-significant.

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