• 한국전자통신학회논문지
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• 제11권8호
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• pp.817-826
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• 2016

생활공간에서 안전을 확보하는 것은 중요하다. 본 연구에서는 기존의 지역별 시간별 사건발생 빈도 기반의 위험 알림 서비스에서 더 확장된 위험도 산출 방안을 제안한다. 시간외에도 생활공간의 조도, 유동인구 뿐 아니라 개인의 상태까지 위험도 산출에 포함하였다. 기존의 확률기반 증거이론은 상황과 관련있는 요소의 수가 증가할 때, 계산량은 급증하는 문제가 있는데 이를 피하기 위하여 퍼지이론을 도입하여 확장성을 확보할 수 있었다. 제안한 산출 방안으로 위험도를 산출한 계산 결과가 언어적 형용 표현까지 가능함을 볼 수 있었다.

• BMB Reports
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• 제42권11호
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• pp.731-736
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• 2009

The generation of functional recombinant antibodies from hybridomas is necessary for antibody engineering. However, this is not easily accomplished due to high levels of aberrant heavy and light chain mRNAs, which require a highly selective technology that has proven complicated and difficult to operate. Herein, we attempt to use an alkaline phosphate (AP)-fused form of single-chain variable fragment (scFv) for the simple identification of a hybridoma-derived, functional recombinant antibody. As a representative example, we cloned the scFv gene from a hybridoma-producing mouse IgG against branched-chain keto acid dehydrogenase complex-E2 (BCKD-E2) into an expression vector containing an in-frame phoA gene. Functional recombinant antibodies were easily identified by conventional enzyme-linked immunosorbent assay (ELISA) by employing scFv-AP fusion protein, which also readily serves as a valuable immuno-detective reagent.

• 대한의생명과학회지
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• 제10권3호
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• pp.179-186
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• 2004

We examined a role of proteasome, the non-lysosomal multicatalytic protease complex,on the differentiation of chick embryonic myoblasts in culture. The peptide hydrolyzing activities of proteasome were found to change; the hydrolyzing activity against N-succinyl-Leu-Leu- Val- Tyr-7 -amido-4-methy1coumarin (SLLVY-AMC) was prominent and increased with myogenic differentiation. Proteasome inhibitors, N-carbobenzoxy-Leu-Leu-norvalinal (MG115) and N-carbobenzoxy-Ile-Glu (O-t-butyl)-Ala-Leucinal (PSI), blocked membrane fusion of myoblasts as well as the SLLVY-AMC hydrolyzing activity. Those inhibitory activities of the agents occurred in parallel, but were reversible and both cell fusion and the peptidase activity were restored when the agents were withdrawn from the culture medium. On the other hand, the agents caused accumulation of the ubiquitinylated proteins in the cytoskeletal proteins. These results suggest that each of the peptide hydrolyzing activities of proteasome is independently regulated during the myogenic differentiation and the chymotrypsin-like activity may play an important role in that process.

• Journal of Korean Neurosurgical Society
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• 제30권4호
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• pp.537-540
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• 2001

Fractures of C1 are not uncommon, constituting only 10% of all cervical spine injuries. There is a high prevalence of concomitant fractures of the second and first cervical vertebral complex. Surgical treatment is controversal. Mainstay of treatment is various combination of traction and cervical orthosis according to degree of displacement and location of fracture. We experienced unusual type of fracture, anterior arch fracture of C1 who had a history of total laminectomy of C1,2 due to cervical cord tumor(neurilemmoma arising from C2 root). We performed C1,2 lateral mass screw fixation with posterior fusion with good postoperative outcome.

• 약학회지
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• 제50권2호
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• pp.93-98
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• 2006

Human foamy virus (HFV) integrase mediates integration of viral c-DNA into cellular DNA. In this process, HFV prointegration complex (PIC) in which integrase is a key component moves to nuclei of the infected cells and leads to integration of viral DNA to the cellular genome, which is essential in viral life cycle. In general nuclear localization signals (NLS) have been suggested to be involved in localizing retroviral PIC to nuclei, but the mechanisms for nuclear localization of the HFV PIC remains unclear. To functionally identify the NLS of HFV integrase, various subdomains of the protein were expressed as GFP fusions and their subcellular locations were analyzed with confocal laser scanning microscopy. Wild type HFV integrase was karyophilic by targeting the fusion protein to nuclei of the COS-1 and 293T cells. Our results showed that strong NLS of HFV integrase was mapped to the C-terminal regions. In addition the karyophilic properties of N-terminal and central regions are not individually strong enough to direct localization of the fusion proteins to nuclei, but their cooperative activity for nuclear import was confirmed.

• Journal of Communications and Networks
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• 제7권4호
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• pp.438-449
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• 2005

In this paper, we describes the information-theoretic approaches to sensor selection and sensor placement in sensor net­works for target localization and tracking. We have developed a sensor selection heuristic to activate the most informative candidate sensor for collaborative target localization and tracking. The fusion of the observation by the selected sensor with the prior target location distribution yields nearly the greatest reduction of the entropy of the expected posterior target location distribution. Our sensor selection heuristic is computationally less complex and thus more suitable to sensor networks with moderate computing power than the mutual information sensor selection criteria. We have also developed a method to compute the posterior target location distribution with the minimum entropy that could be achieved by the fusion of observations of the sensor network with a given deployment geometry. We have found that the covariance matrix of the posterior target location distribution with the minimum entropy is consistent with the Cramer-Rao lower bound (CRB) of the target location estimate. Using the minimum entropy of the posterior target location distribution, we have characterized the effect of the sensor placement geometry on the localization accuracy.

• 한국멀티미디어학회논문지
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• 제21권7호
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• pp.764-771
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• 2018

In this paper, we propose a high-quality image acquisition algorithm using only a single image, which the high-quality image is normally referred as HDR ones. In order to acquire the HDR image, conventional methods need many images having different exposure values at the same scene and should delicately adjust the color values for a bit-expansion or an exposure fusion. Thus, they require considerable calculations and complex structures. Therefore, the proposed algorithm suggests a completely new approach using one image for the high-quality image acquisition by applying statistical difference and histogram manipulation, or histogram specification, techniques. The techniques could control the pixel's statistical distribution of the input image into the desired one through the local and the global modifications, respectively. As the result, the quality of the proposed algorithm is better than those of conventional methods implemented in commercial image editing softwares.

• Journal of Welding and Joining
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• 제28권2호
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• pp.47-53
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• 2010

According to the research report for the recent a few years, the dissimilar welding of Cu and STS 304 alloy have been presented that a weldability is very poor. This article present a study on Lap joint by Electron beam welding dissimilar materials. The weld metals was constituted between pure copper and STS 304 steel. The experiment was performed with 125mA welding current, 520mA focusing current. The Vacuum condition of chamber is 5${\times}$10-5torr and welding speed is 300mm/min. Showing the bead shape of weld metal, the thickness of the stainless 304 using as the protect materials is 3mm and the thickness of a copper is 15mm. The analysis about the microstructure were carried out in which it was observed with SEM. The results showed that complex heterogeneous fusion zone microstructure characterized both by rapid cooling and mixing of the molten metal, however the liquation crack was formated in the fusion line.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2004년도 ICEIC The International Conference on Electronics Informations and Communications
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• pp.237-243
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• 2004

A multichannel smart sound sensor capable to detect and identify sound events in noisy conditions is presented in this paper. Sound information extraction is a complex task and the main difficulty consists is the extraction of high­level information from an one-dimensional signal. The input of smart sound sensor is composed of data collected by 5 microphones and its output data is sent through a network. For a real time working purpose, the sound analysis is divided in three steps: sound event detection for each sound channel, fusion between simultaneously events and sound identification. The event detection module find impulsive signals in the noise and extracts them from the signal flow. Our smart sensor must be capable to identify impulsive signals but also speech presence too, in a noisy environment. The classification module is launched in a parallel task on the channel chosen by data fusion process. It looks to identify the event sound between seven predefined sound classes and uses a Gaussian Mixture Model (GMM) method. Mel Frequency Cepstral Coefficients are used in combination with new ones like zero crossing rate, centroid and roll-off point. This smart sound sensor is a part of a medical telemonitoring project with the aim of detecting serious accidents.

• 대한금속재료학회지
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• 제49권6호
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• pp.454-461
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• 2011

In this study, the relationship between corrosion resistance and microstructural characteristics such as grain size reduction, preferred orientation, and homogenous distribution of elements and impurity by mechanical milling of magnesium powder was investigated. Mechanical milling of pure magnesium powder exhibited a complex path to grain refinement and growth together with preferred orientation reversal with milling time. It was also found that anisotropic formation of dislocation on the basal plane of magnesium was initially the dominant mechanism for grain size reduction. After 60 hrs of milling, grain coarsening was observed and interpreted as a result of the strain relaxation process through recrystallization. In spite of the finer grain size and strong (002) texture developed in the sample prepared by spark plasma sintering at $500^{\circ}C$ for 5 min after mechanical milling for 2hrs, the sample showed a higher corrosion rate. The results from this study will be helpful for better understanding of the controlling factor for corrosion resistance and behaviors of mechanical milled magnesium powders.