• Journal of Microbiology
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• v.34 no.1
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• pp.7-14
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• 1996

Teh baculovirus gp64 glycoprotein is a major component of the envelope of budded virus (BV) and has been shown that it plays an essential role in the infection process, especially virus-cell membrane fusion. We have cloned Autographa californica Nuclear Polyhedrosis Virus (AcNPV) gp64 protein were examined for membrane fusion activity by using a synchtium formation assay under various conditions. The optimal conditions required for inducing membrane fusion are 1) form pH 4.0 to 4.8 2) 15 min exposure of cells to acidic pH 3) at least 1 .mu.g of gp64 cloned plasmid DNA per 3 * 10$^{6}$ cells 4) and an exposure of cells to acidic pH at 72 h post-transfection. In order to investigate the role of hydrophobicity of the gp64 glycoprotein for the membrane fusion, the two leucine residues (amino acid position at 229 and 230) within hydrophobic region I were substituted to alanine by PCR-derived site-directed mutagenisis and the membrane fusion activity of the mutant was anlaysed. The gp64 glycoprotein carrying double alamine substitution mutation showed no significant difference in fusion activity. This result suggested that minor changes in hydrophobicity at the amino acid position 229 and 230 does not affect the acid-induced membrane fusion activity of the gp64 glycoprotein.

• ETRI Journal
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• v.33 no.4
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• pp.497-505
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• 2011

Image fusion is a technical method to integrate the spatial details of the high-resolution panchromatic (HRP) image and the spectral information of low-resolution multispectral (LRM) images to produce high-resolution multispectral images. The most important point in image fusion is enhancing the spatial details of the HRP image and simultaneously maintaining the spectral information of the LRM images. This implies that the physical characteristics of a satellite sensor should be considered in the fusion process. Also, to fuse massive satellite images, the fusion method should have low computation costs. In this paper, we propose a fast and efficient satellite image fusion method. The proposed method uses the spectral response functions of a satellite sensor; thus, it rationally reflects the physical characteristics of the satellite sensor to the fused image. As a result, the proposed method provides high-quality fused images in terms of spectral and spatial evaluations. The experimental results of IKONOS images indicate that the proposed method outperforms the intensity-hue-saturation and wavelet-based methods.

• Journal of Powder Materials
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• v.30 no.2
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• pp.146-155
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• 2023

The Ti-6Al-4V lattice structure is widely used in the aerospace industry owing to its high specific strength, specific stiffness, and energy absorption. The quality, performance, and surface roughness of the additively manufactured parts are significantly dependent on various process parameters. Therefore, it is important to study process parameter optimization for relative density and surface roughness control. Here, the part density and surface roughness are examined according to the hatching space, laser power, and scan rotation during laser-powder bed fusion (LPBF), and the optimal process parameters for LPBF are investigated. It has high density and low surface roughness in the specific process parameter ranges of hatching space (0.06-0.12 mm), laser power (225-325 W), and scan rotation (15°). In addition, to investigate the compressive behavior of the lattice structure, a finite element analysis is performed based on the homogenization method. Finite element analysis using the homogenization method indicates that the number of elements decreases from 437,710 to 27 and the analysis time decreases from 3,360 to 9 s. In addition, to verify the reliability of this method, stress-strain data from the compression test and analysis are compared.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.3
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• pp.296-305
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• 2017

This study aims to create a new urban space through mechanism structure and algorism analysis between IT and ET. The results are as follows. First, the development trends of ET IT are classified into 4 types, "Eco-Friendly Development", "Energy Production Technology Development", "Energy Saving Technology Development" and "Wide Area IT Network Development", which are found to be constantly evolving. Second, Sang-Am DMC developed through the environmentally protective and eco-friendly aspects of ET from the Korean War to 1978. Wide area IT networks developed rapidly from 1990 to 2000. However, in 2010, urban spaces began to develop by the fusion of the Environment and Information. The fusion of Environment and Information in the development trends in the past is referred to as "Individual Development", that in the present is referred to as "Semi-fusion Development" and that in the future will be referred to as "Total Fusion Development". Third, the mechanism structure of DMC has evolved through creation, extinction and fusion processes. The creation process will serve to supplement the insufficiencies of the existing systems, the extinction process will be the compactification of the fusion process, and the fusion process will be the standard for creation and extinction. Finally, the future of new innovative urban and architectural spaces will be forged by the mechanism symbolization patterns of IT ET.

• 제어로봇시스템학회:학술대회논문집
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• 2001.10a
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• pp.91.3-91
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• 2001

A sensor fusion scheme for mobile robot environment recognition that incorporates range data and contour data is proposed. Ultrasonic sensor provides coarse spatial description but guarantees open space with no obstacle within sonic cone with relatively high belief. Laser structured light system provides detailed contour description of environment but prone to light noise and is easily affected by surface reflectivity. Overall fusion process is composed of two stages: Noise elimination and belief updates. Dempster Shafer´s evidential reasoning is applied at each stage. Open space estimation from sonar range measurements brings elimination of noisy lines from laser sensor. Comparing actual sonar data to the simulated sonar data enables ...

• Journal of Embryo Transfer
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• v.26 no.4
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• pp.287-296
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• 2011

Mammalian fertilization is a complex cascade process consisting of sperm migration through the female reproductive tract, physiological changes to sperm such as sperm capacitation and acrosome reaction, and sperm-egg interaction in the oviduct in vivo. On the other hand, in vitro fertilization (IVF) is a process by which egg cells are fertilized by sperm outside the body: in vitro. IVF has been used for a variety of purposes in reproductive biotechnology for human and animals. The discovery of sperm capacitation in 1951 promoted the development of IVF technology. In the initial stage of IVF, sperm capacitation in preincubation medium was shown to be essential to fuse with eggs. Besides, sperms should detour some of the in vivo regulations for IVF. This review introduces a general mammalian fertilization process, including sperm capacitation, removal of cumulus matrix, acrosome reaction, and sperm-egg fusion and focuses on the roles of key biochemical molecules, signal mechanisms, and genes involved during IVF and novel results of sperm-oocyte interaction elucidated in various gene-knockout mice models.

• Proceedings of the KSRS Conference
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• v.2
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• pp.639-642
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• 2006

The intensity-hue-saturation (IHS) technique has become a standard procedure in image analysis. It enhances the colour of highly correlated data. Unfortunately, IHS technique is sensitive to the properties of the analyzed area and usually faces colour distortion problems in the fused process. This paper explores the relationship of colour between before and after the fused process and the change in colour space of images. Subsequently, the fused colours are transformed back into the 'simulative' true colours by the following steps: (1) For each pixel of fused image that match with original pixel (of the coarse spectral resolution image) is transformed back to the true colour of original pixel. (2) The value for interpolating pixels is compensated to preserve the DN ratio between the original pixel and it's vicinity. The 'compensative matrix' is constructed by the DN of fused images and simulation of scaling process. An illustrative example of a Landsat and SPOT fused image also demonstrates the simulative true colour fusion methods.

• Korean Journal of Metals and Materials
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• v.49 no.8
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• pp.649-656
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• 2011

The present study evaluated the microstructures and mechanical properties of severely deformed Ni-30Cr alloys. Cross-roll rolling (CRR) process was introduced as a severe plastic deformation (SPD), and Ni-30Cr alloy sheets were cold rolled to 90% thickness reduction and subsequently annealed at $700^{\circ}C$ for 30 min to obtain the recrystallized microstructure. Electron back-scattering diffraction (EBSD) was introduced to analyze grain boundary character distributions (GBCDs). The application of CRR to the Ni-30Cr alloy was effective in enhancing the grain refinement through heat treatment; consequently, the average grain size was significantly refined from $33{\mu}m$ in the initial material to $0.6{\mu}m$. This grain refinement directly improved the mechanical properties, in which yield and tensile strengths significantly increased relative to those of the initial material. We systematically discuss the grain refinement and accompanying improvement of the mechanical properties, in terms of the effective strain imposed by CRR relative to conventional rolling (CR).

• Proceedings of the Korea Contents Association Conference
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• 2006.11a
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• pp.702-705
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• 2006

Because of explosive increase of information, it spends a lot of time to trace and analysis research trends during total R&D process. It has rapidly evolved from R&D or process development within a specific domain of knowledge to R&D or process development through knowledge convergency. To accept such a paradigm, it is necessary to convert dissemination system from a separate, standalone, and fragmentary information to highly coupled fusion information. Although there are several studies on knowledge flows between science and technology or technology and industry, it is difficult to analyse and utilize quantitatively to establish policy of Science, Technology, and Industry. The reason is the lack of information resource to analyse knowledge flow from science to industry. This paper intends to develop framework of highly coupled linking system between patent and scientific paper to utilize R&D, S&T policy, and industry policy.

• Korean Chemical Engineering Research
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• v.55 no.4
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• pp.561-566
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• 2017

Butt-fusion welding process is used to join the polymeric pipes. Recently, some researchers suggest the curved surface to enhance a welding quality. We investigated how curved welding surface affects heat and flow behaviors of polymer melt during the process in 2D axisymmetric domain with finite element method, and discussed the effect to the welding quality. In this study, we considered HDPE pipes. In heat soak stage, curved phase interface between the melt and solid is shown along the shape of welding surface. In jointing stage, squeezing flow is generated between curved welding surface and phase interface. The low shear rate in fusion domain reduces the alignment of polymer to the perpendicular direction of pipes, and then this phenomenon is expected to help to enhance the welding quality.