• Title/Summary/Keyword: Fusion Mechanism

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Plant defense signaling network study by reverse genetics and protein-protein interaction

  • Paek, Kyung-Hee
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.29-29
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    • 2003
  • Incompatible plant-pathogen interactions result in the rapid cell death response known as hypersensitive response (HR) and activation of host defense-related genes. To understand the molecular and cellular mechanism controlling defense response better, several approaches including isolation and characterization of novel genes, promoter analysis of those genes, protein-protein interaction analysis and reverse genetic approach etc. By using the yeast two-hybrid system a clone named Tsipl, Tsil -interacting protein 1, was isolated whose translation product apparently interacted with Tsil, an EREBP/AP2 type DNA binding protein. RNA gel blot analysis showed that the expression of Tsipl was increased by treatment with NaCl, ethylene, salicylic acid, or gibberellic acid. Transient expression analysis using a Tsipl::smGFP fusion gene in Arabidopsis protoplasts indicated that the Tsipl protein was targeted to the outer surface of chloroplasts. The targeted Tsipl::smGFP proteins were diffused to the cytoplasm of protoplasts in the presence of salicylic acid (SA) The PEG-mediated co-transfection analysis showed that Tsipl could interact with Tsil in the nucleus. These results suggest that Tsipl-Tsil interaction might serve to regulate defense-related gene expression. Basically the useful promoters are valuable tools for effective control of gene expression related to various developmental and environmental condition.(중략)

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A Study on Collective Intelligence and Process Coach (집단지성과 프로세스 코치 연구)

  • Hong, Sam-Yull
    • The Journal of the Korea institute of electronic communication sciences
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    • v.10 no.4
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    • pp.533-538
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    • 2015
  • Collective intelligence is related to several areas such as sociology, business administration, political science, and computer science. This paper can be classified as a product of social engineering of the era of liberal arts and science convergence, fusion, consilience. Members today have higher need for self-actualization and contribution. As the business is changing fast and getting more complicated, a mechanism of natural science is necessary in social organization. The mechanisms of collective intelligence are composed of divergence process and convergence process. And the seven steps were designed that the first letter of each steps leads to 'PROCESS'. When implemented by applying the procedures that reflect the opinions of members throughout this paper, there are members who participated in the decision-making process will contribute to actively participate in the decision when to run, and specific tools and techniques in online communities are for future studies.

Synchronous 2D/3D Switching System for Service-Compatible 3DTV Broadcasting

  • Kim, Sangjin;Jeon, Taehyun
    • ETRI Journal
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    • v.36 no.2
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    • pp.271-279
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    • 2014
  • This paper proposes a new broadcasting system for the service-compatible 3DTV in which the 3D service can coexist with the conventional digital TV broadcast. In the proposed system, the commercial 3DTV service can be implemented via the existing DTV channel without utilizing the dedicated 3DTV system. This 2D/3D system interworks with the conventional system and can switch to 2D or 3D service according to the broadcast programming and schedule. The system also provides a mechanism that can prevent the synchronization mismatch between left and right video streams and between the stream and the associated signaling in the 2D/3D transition periods. The picture quality measurements are carried out based on the ITU-R recommended test to check the level of quality of service provided by the proposed scheme. The conformity tests are also performed with the conventional channel and the receiver for the DTV system to confirm the feasibility of the proposed one for the commercial service.

A Case of Ring Chromosome 20 with Mental Retardation and Epilepsy (정신 지체와 간질을 동반한 20 환(Ring) 염색체 증후군 1례)

  • Jung, Yeon Kyung;Lee, Gyeong Hoon
    • Clinical and Experimental Pediatrics
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    • v.48 no.1
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    • pp.108-111
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    • 2005
  • Ring chromosome 20 mosaicism [r(20)] is a rare chromosomal anomaly associated with minor dysmorphism, mental retardation, autistic behavior, and intractable epilepsy. The proposed mechanism of ring formation is breakage of both short and long arms of a chromosome with subsequent end-to-end fusion. We encountered an 18-month-old boy who presented with developmental delay and mental retardation with seizure episodes, but showed normal brain magnetic resonance imaging. Chromosome study from peripheral blood showed 46,XY, r(20)(p13q13.3) karyotype. The authors report a case of ring chromosome 20 with mental retardation and epilepsy, with a review of the literature.

Identification and Characterization of Protein Arginine Methyltransferase 1 in Acanthamoeba castellanii

  • Moon, Eun-Kyung;Kong, Hyun-Hee;Hong, Yeonchul;Lee, Hae-Ahm;Quan, Fu-Shi
    • Parasites, Hosts and Diseases
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    • v.55 no.2
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    • pp.109-114
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    • 2017
  • Protein arginine methyltransferase (PRMT) is an important epigenetic regulator in eukaryotic cells. During encystation, an essential process for Acanthamoeba survival, the expression of a lot of genes involved in the encystation process has to be regulated in order to be induced or inhibited. However, the regulation mechanism of these genes is yet unknown. In this study, the full-length 1,059 bp cDNA sequence of Acanthamoeba castellanii PRMT1 (AcPRMT1) was cloned for the first time. The AcPRMT1 protein comprised of 352 amino acids with a SAM-dependent methyltransferase PRMT-type domain. The expression level of AcPRMT1 was highly increased during encystation of A. castellanii. The EGFP-AcPRMT1 fusion protein was distributed over the cytoplasm, but it was mainly localized in the nucleus of Acanthamoeba. Knock down of AcPRMT1 by synthetic siRNA with a complementary sequence failed to form mature cysts. These findings suggested that AcPRMT1 plays a critical role in the regulation of encystation of A. castellanii. The target gene of AcPRMT1 regulation and the detailed mechanisms need to be investigated by further studies.

A Review on VOCs Control Technology Using Electron Beam

  • Son, Youn-Suk;Kim, Ki-Joon;Kim, Jo-Chun
    • Asian Journal of Atmospheric Environment
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    • v.4 no.2
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    • pp.63-71
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    • 2010
  • The removal characteristics for aromatic and aliphatic VOCs by electron beam (EB) were discussed in terms of several removal variables such as initial VOC concentration, absorbed dose, background gas, moisture content, reactor material and inlet temperature. It was reviewed that only reactor material was an independent variable among the potential control factors concerned. It was also suggested that main mechanism by EB should be radical reaction for the VOC removal rather than that by primary electrons. It was discussed that the removal efficiency of benzene was lower than that of hexane due to a closed benzene ring. In the case of aromatic VOCs, it was observed that the decomposition of the VOCs with more functional groups attached on the benzene ring was much easier than those with less ones. As for aliphatic VOCs, it was also implied that the longer carbon chain was, the higher the removal efficiency became. An EB-catalyst hybrid system was discussed as an alternative way to remove VOCs more effectively than EB-only system due to much less by-products. This hybrid included supporting materials such as cordierite, Y-zeolite, and $\gamma$-alumina.

Identification of An Antibacterial Gene by Differential Display from Lipopolysaccharide-Stimulated Dung Beetle, Copris tripartitus

  • Suh, Hwa-Jin;Kim, Yeon-Ju;Bang, Hea-Son;Yun, Eun-Young;Kim, Seong-Ryul;Park, Kwan-Ho;Kang, Bo-Ram;Kim, Ik-Soo;Jeon, Jae-Pil;Hwang, Jae-Sam
    • International Journal of Industrial Entomology and Biomaterials
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    • v.17 no.2
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    • pp.223-228
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    • 2008
  • A novel beetle antimicrobial protein from stimulated Copris tripartitus and the corresponding gene were isolated in parallel through differential display-PCR and expression in Escherichia coli. To find cDNA clones responsible for bacteria resistance, the suppression subtractive hybridization and GeneFishing differentially expressed genes system were employed in the dung beetle, Copris tripartitus immunized with lipopolysaccaride. One cDNA clone from eight subtracted clones was selected through dot blot analysis and confirmed by northern blot analysis. The 516-bp, selected cDNA clone was determined by 5' and 3' rapid amplication of cDNA ends and cloned into the GST fusion expression vector pGEX-4T-1 for expression of the protein. The expressed protein was predicted 14.7 kDa and inhibited the growth of gram-negative bacteria such as Escherichia coli and Pseudomonas aeruginosa. These results implied that the expressed protein is related to immune defense mechanism against microorganism.

Dynamics of OH Production in the Reaction of O(1D2) with Cyclopropane

  • Jang, Sungwoo;Jin, Sung Il;Kim, Hong Lae;Kim, Hyung Min;Park, Chan Ryang
    • Bulletin of the Korean Chemical Society
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    • v.35 no.6
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    • pp.1706-1712
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    • 2014
  • The OH($X^2{\Pi}$, ${\upsilon}^{\prime\prime}=0,1$) internal state distribution following the reaction of electronically excited oxygen atom ($O(^1D_2)$) with cyclo-$C_3H_6$ has been measured using laser-induced fluorescence, and compared with that following the reaction of $O(^1D_2)$ with $C_3H_8$. The overall characteristics of the OH internal energy distributions for both reactions were qualitatively similar. The population propensity of the ${\Pi}(A^{\prime})$ ${\Lambda}$-doublet sub-level suggested that both reactions proceeded via an insertion/elimination mechanism. Bimodal rotational population distributions supported the existence of two parallel mechanisms for OH production, i.e., statistical insertion and nonstatistical insertion. However, detailed analysis revealed that, despite the higher exoergicity of the reaction, the rotational distribution of the OH following the reaction of $O(^1D_2)$ with $C_3H_8$ was significantly cooler than that with cyclo-$C_3H_6$, especially in the vibrational ground state. This observation was interpreted as the effect of the flexibility of the insertion complex and faster intramolecular vibrational relaxation (IVR).

A Basic Study for Smart Zero Carbon Cities (스마트 저탄소도시를 위한 기초연구)

  • Shin, Wan Sun;Choi, Seong Ho;Park, Jin Chul;Song, Yong Woo
    • Land and Housing Review
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    • v.10 no.1
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    • pp.19-23
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    • 2019
  • In recent years, many studies have been conducted on smart low carbon cities through the fusion of ICT information technology for the purpose of reducing carbon. In this study, we investigated 13 cities in three continents that implement low-carbon city policies and analyzed the size, economic and social characteristics of each city to identify the degree of dynamic mechanism for carbon reduction. To this end, we quantified the elements of the city and analyzed the basic requirements for low-carbon cities using the TOPSIS method. The study found that most cities were better able to activate institutions and cultural conditions, facilities and functional conditions, and economic and industrial conditions than other engines, and these three were the main forms of power for smart low carbon cities. The results of this study are expected to be used as a basis for suggesting policy recommendations and improvement measures for future smart low carbon cities.

Study on Process Parameters for Effective H2 Production from H2O in High Frequency Inductively Coupled Plasma Reactor (고주파유도결합플라즈마 반응기에서 물로부터 수소생성효율을 높이기 위한 공정변수에 대한 연구)

  • Kwon, Sung-Ku;Jung, Yong-Ho
    • Journal of Hydrogen and New Energy
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    • v.22 no.2
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    • pp.206-212
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    • 2011
  • The effect of process parameters on $H_2$ production from water vapor excited by HF ICP has been qualitatively examined for the first time. With the increase of ICP power, characteristics of $H_2$ production from $H_2O$ dissociation in plasma was divided into 3 regions according to both reaction mechanism and energy efficiency. At the edge of region (II) in the range of middle ICP power, energy effective hydrogen production from $H_2O$ plasma can be achieved. Furthermore, within the region (II) power condition, heating of substrate up to $500^{\circ}C$ shows additional increase of 70~80% in $H_2$ production compared to $H_2O$ plasma without substrate heating. This study have shown that combination of optimal plasma power (region II) and wall heating (around $500^{\circ}C$) is one of effective ways for $H_2$ production from $H_2O$.