• KSBB Journal
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• v.27 no.3
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• pp.186-194
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• 2012

Various human host cell lines, which are more effective than the other original human cell lines, have been developed and used. Highly efficient human cell line can be obtained from the fusion between human embryonic kidney 293 (HEK293) and human Burkitt's lymphoma cells (Namalwa). Fused cell line has the advantages of both cell lines such as the high transfection efficacy of HEK293 cells and the constitutive expression of Epstein-Barr virus (EBV) genome which is related with high expression of target protein and anti-apoptotic growth of Namalwa cells. In this study, characterization of two original cell lines was performed by using design of experiment (DOE) considering cell maintenance, media development, optimization of culture condition, and scale-up. The formation of aggregates was apparent with high glutamine concentration at more than 6 mM. Supplementation of hydrolysates showed positive effects on the growth performances of HEK293 cells. On the contrary, Namalwa cells showed negative results. It was confirmed that Namalwa cells were more sensitive to lower temperature at $35^{\circ}C$ and hyperosmotic condition over 260 mOsm/kg. In addition, both cell lines showed limited growth in 3-L bioreactor due to shear stress.

• The Journal of the Korea institute of electronic communication sciences
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• v.9 no.12
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• pp.1415-1420
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• 2014

3D printer is based on an additive manufacturing technology, which helps in creating the three-dimensional object using a 3D drawing. It is used in various fields, because it prints out a variety of three-dimensional products in a short period of time. In this paper, we consider a technique using the FDM(Fused Deposition Modeling) method by dissolving the ABS(Acrylonitrile Butadiene Styrene) resin among a diversity of printing technique and materials. This kind of the 3D printer prints out a product in high temperature and cools down it. In this process, a flection phenomenon is occurred according to the size of the printing product and the surrounding environment. Conventional methods for mitigating this phenomenon maintain the temperature at the optimum level, but they require using additional devices. In order to minimize the flection phenomenon in 3D printing products without additional devices, in this paper, we propose a noble technique, which creates holes on suitable positions when they are designed by 3D drawing tools. Also, we suggest mathematical model for the proposed method, and measure and analyse a printing output using a proposed technique.

• Journal of Technologic Dentistry
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• v.31 no.3
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• pp.27-34
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• 2009

This study compared and analyzed changes to the mechanical characteristics to nonprecious metal alloy for porcelain fused to metal crowns when it's repetitively used without the addition of new alloy. Metal samples were made with the Verabond V nonprecious metal alloy. Those samples to measure tensile and yield strength were made in the standardized design(ISO 22674), those to measure bond strength in the $25mm{\times}3mm{\times}0.5mm$ format, and those to measure hardness in the $10mm{\times}10mm{\times}1mm$ format. A ceramic to measure bond strength was made at the center of the metal sample in the length of $8{\ss}{\AE}$ by using Noritaker Super Porcelain EX-3. Ten samples were prepared for one, three and five repetitions of casting each. The test results were as follows: 1. The more casting was repeated, the more significantly tensile strength dropped. 2. The more casting was repeated, the more significantly yield strength dropped. 3. Repetitive casting didn't cause significant changes to bond strength. 4. The Vickers hardness significantly fell with increasing repetitions of casting. There were no changes to bond strength observed with the increasing number of repeating casting. But tensile strength, yield strength, and Vickers hardness decreased. Those results indicate that repeated casting can affect durability and that careful attention should be paid by avoiding repetitive use or excessive increase of uses when no new alloy is added.

• Journal of Microbiology and Biotechnology
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• v.29 no.5
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• pp.813-819
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• 2019

Middle East respiratory syndrome coronavirus (MERS-CoV) induces severe respiratory impairment with a reported mortality rate of ~36% in humans. The absence of clinically available MERS-CoV vaccines and treatments to date has resulted in uncontrolled incidence and propagation of the virus. In vaccine design, fusion with the IgG Fc domain is reported to increase the immunogenicity of various vaccine antigens. However, limited reports have documented the potential negative effects of Fc fusion on vaccine antigens. To determine whether Fc fusion affects the immunogenicity of MERS-CoV antigen, we constructed a Fcassociated MERS-CoV spike protein (eS770-Fc, 110 kDa), whereby human IgG4 Fc domain was fused to MERS-CoV spike protein (eS770) via a Gly/Pro linker using baculovirus as the expression system. For comparative analyses, two eS770 proteins lacking the IgG4 Fc domain were generated using the IdeS protease ($eS770-{\Delta}Fc$) or His tag attachment (eS770-His) and the immunogenicity of the above constructs were examined following intramuscular immunization in mice. Contrary to expectations, non-Fc spike proteins ($eS770-{\Delta}Fc$, eS770-His; 90 kDa) showed higher immunogenicity than the Fc fusion protein (eS770-Fc). Moreover, unlike non-Fc spike proteins, eS770-Fc immunization did not elicit neutralizing antibodies against MERS-CoV. The lower immunogenicity of Fc-fused eS770 was related to alterations in the structural conformation of the spike protein. Taken together, our results indicate that IgG Fc fusion reduces the immunogenicity of eS770 by interfering with the proper folding structure.

• Ocean Systems Engineering
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• v.12 no.2
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• pp.225-245
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• 2022

More and more shipping containers are falling into the sea due to bad weather. Containers lost at sea negatively affect the shipping line, the trader and the consumer, and the environment. The question of locating and recovering dropped containers is a challenging engineering problem. Model-testing of small-scaled container models is proposed as an efficient way to investigate their falling trajectories to salvage them. In this study, we first build a standard 20-ft container model in SOLIDWORKS. Then, a three-dimensional (3D) geometric model in the STL (Standard Tessellation Language) format is exported to a Stratasys F170 Fused Deposition Modeling (FDM) printer. In total, six models were made of acrylonitrile styrene acrylate (ASA) and printed for the purpose of testing. They represent three different loading conditions with different densities and center of gravity (COG). Two samples for each condition were tested. The physical models were dropped into the towing tank of University of New Orleans (UNO). From the experimental tests, it is found that the impact of the initial position after sinking can cause a certain initial rolling velocity, which may have a great impact on the lateral displacement, and subsequently affect the final landing position. This series of model tests not only provide experimental data for the study of the trajectory of box-shape objects but also provide a valuable reference for maritime salvage operations and for the pipeline layout design.

• Asian Spine Journal
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• v.12 no.6
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• pp.1060-1068
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• 2018

Study Design: Retrospective study. Purpose: Lumbar intervertebral disc degeneration is an important cause of low back pain. Overview of Literature: Spinal fusion is often reported to have a good course for adolescent idiopathic scoliosis (AIS). However, many studies have reported that adjacent segment degeneration is accelerated after lumbar spinal fusion. Radiography is a simple method used to evaluate the orientation of the vertebral column. magnetic resonance imaging (MRI) is the method most often used to specifically evaluate intervertebral disc degeneration. The Pfirrmann classification is a well-known method used to evaluate degenerative lumbar disease. After spinal fusion, an increase in stress, excess mobility, increased intra-disc pressure, and posterior displacement of the axis of motion have been observed in the adjacent segments. Methods: we retrospectively secured and analyzed the data of 15 patients (four boys and 11 girls) with AIS who underwent a spinal fusion surgery. We studied the full-length view of the spine (anterior-posterior and lateral) from the X-ray and MRI obtained from all patients before surgery. Postoperatively, another full-length spine X-ray and lumbosacral MRI were obtained from all participants. Then, pelvic tilt, sacral slope, curve correction, and fused and free segments before and after surgery were calculated based on X-ray studies. MRI images were used to estimate the degree to which intervertebral discs were degenerated using Pfirrmann grading system. Pfirrmann grade before and after surgery were compared with Wilcoxon signed rank test. While analyzing the contribution of potential risk factors for the post-spinal fusion Pfirrmann grade of disc degeneration, we used generalized linear models with robust standard error estimates to account for intraclass correlation that may have been present between discs of the same patient. Results: The mean age of the participant was 14 years, and the mean curvature before and after surgery were 67.8 and 23.8, respectively (p<0.05). During the median follow-up of 5 years, the mean degree of the disc degeneration significantly increased in all patients after surgery (p<0.05) with a Pfirrmann grade of 1 and 2.8 in the L2-L3 before and after surgery, respectively. The corresponding figures at L3-L4, L4-L5, and L5-S1 levels were 1.28 and 2.43, 1.07 and 2.35, and 1 and 2.33, respectively. The lower was the number of free discs below the fusion level, the higher was the Pfirrmann grade of degeneration (p<0.001). Conversely, the higher was the number of the discs fused together, the higher was the Pfirrmann grade. Conclusions: we observed that the disc degeneration aggravated after spinal fusion for scoliosis. While the degree of degeneration as measured by Pfirrmann grade was directly correlated by the number of fused segments, it was negatively correlated with the number of discs that remained free below the lowermost level of the fusion.

• Korean Institute of Interior Design Journal
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• v.18 no.5
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• pp.71-79
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• 2009

This research understands semantics-system in contemporary space design as a poetic form. It provides that the possibility of various interpretation of space and makes to escape from insipid formal logic of compulsion uniform meaning. In order to unfold this argument, poetic semantics system has to be revealed using conversion of articulation factors in text of space(semantics and syntax). First of all, after setting up the articulation system of space language, we have to understand the conversion of articulation factors that generate a new grammar breaking up the rule of old syntax. And the various expression of form in Contemporary Space design focuses on a poetic expression, that is, the abstraction system fused by space factors(conversion of articulation system). In this method of research to recognize the subject of space in architecture, the importance of interpretation has to be highlighted, as the importance of language is emphasized that intermediates between object and interpretation. The reason to recognize Contemporary space design as a text is that it is a gathering of symbol as a object of interpretation and a mediator. The important issue of this study is to research how and what to transmit by poetic semantics system in contemporary space design. It brings about a poetic problem what it intends to becomes(the problem of meaning operation) in a narrow sense and a interpretational problem what it intends to do(the problem of communication). When we define interpretation the technique of defining a text, it involves the premise of inevitableness of multiple understanding, or the possibility to Interpret variously. In the end the ambiguity of poetic language and the infinity of moaning process as the moaning expansion system in contemporary space design is the flexible measure to solve the self-criticism.

• Journal of the Society of Naval Architects of Korea
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• v.58 no.5
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• pp.294-302
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• 2021

In this study, the 3D printing technique called design for additive manufacturing (DfAM) that is widely used in various industries was applied to marine leisure ships of equipment. The DfAM for the stanchion for crew safety was applied to the equipment used in an actual recreational craft. As design constraints, the design alternatives were not to exceed the safety and weight of the existing stainless steel material, which were reviewed, and the production of a low-cost FFF-type 3D printing method that can be used even in small shipyards was considered. Until now, additive manufacturing has been used for manufacturing only prototypes owing to its limitations of high manufacturing cost and low strength; however, in this study, it was applied to the mass production process to replace existing products. Thus, a design was developed with low manufacturing cost, adequate performance maintenance, and increased design freedom, and the optimal design was derived via structural analysis comparisons for each design alternative. In addition, a life-cycle assessment based on the ISO 1404X was conducted to develop sustainable products. Through this study, the effectiveness of additive manufacturing was examined for future applications in the shipbuilding industry.

• Korean Institute of Interior Design Journal
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• v.24 no.2
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• pp.88-99
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• 2015

As architecture is designed for humans to live in based on practicality, what one experiences within such spaces is important. Such experiences are not felt only by appreciating the structure's appearance, but involve physical presence inside the space and the deepening of the individuals relationship with the space through the body's sensory perception. However, in modern times there is a much greater tendency to rely on superficial experiences by way of images or tastes rather than more meaningful human participation, while the standardization of the object universe is gradually erasing the uniqueness of experience. In light of this, the restoration of emotional and sensory spatial experiences through the design of sensory spaces can be regarded as an important task in modern spatial design. This study contemplated the meaning of emotional spatial experiences through a phenomenological understanding of such experiences, and analyzed the meaning and main factors in the creation of spatial atmosphere as a phenomenon of spatial experience. In addition, we highlighted the fact that spatial atmosphere can be applied as space design methodology by devising methods of expression through an analysis of actual examples. O.F. Bollnow's space theory and the spatial design of Peter Zumthor were the subjects of this analysis. The spatial atmosphere analyzed using Bullnow's theory is a phenomenon in which both the human and space are connected and the ensuing spatial experience embodies the emotions of both. This spatial atmosphere can be understood as a condition in which the human and space are fused together. Furthermore, coincidental/accidental meeting, mediums using potential objects, reorganization of the center due to autonomous boundaries, as well as the three temporalities were identified as the main factors in the creation of spatial atmosphere. Based on this analysis, through the identification of methods of expression for spatial atmosphere in Zumthor's spaces.

• Journal of the Korean Society of Costume
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• v.52 no.5
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• pp.155-172
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• 2002

The research has the aim of defining the special characteristics of the functionalist fashion design in the 1900s by understanding the essence of functionalism in the sociocultural contexts. Giving careful consideration to functionalist fashion design in the 1990s makes it possible to foresee the way of fashion design to meet the needs of the modems and men of the future world. To obtain the purposes, documentary study and demonstrative study with fashion photographs have been executed. Main results are as follows. Functionalism in the 1990s has been developed in different ways from the analogies in the beginning of the 20th century. It doesn't belong to an analogy but has the fused characteristics. It can be classified into techno functionalism influenced by new technology Pure functionalism related to minimalism. casual functionalism for those who are crazy about the speed and sports and enjoy the freedom and comfort and symbolic functionalism. Mechanical analogy has been developed into techno functionalism. Pure functionalism has come from the blend of mechanical and moral analogy, casual functionalism from mechanical. organic and moral analogy. and symbolic functionalism from organic and moral analogy. Since fashion design has much closer relation to human body and movements. it has been expressed considering a division of men and women and the places and times for the designs more than other designs. More casual manners of formal wear are distinct elements in functionalist fashion design in the 1990s. The 20th century general cultural phenomena including post modernism can be explained by eclectic functionalism. Functionalism considering Practical and conceptional functions ill continue and techno functionalism and casual functionalism in future fashion design will be expressed more strongly with new technology and casual trends of life style.