• 미생물학회지
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• 제7권3호
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• pp.107-114
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• 1969

Aspergillus을 이용하요 생리적 제성질을 조사하였던 바 다음과 같은 결론을 얻었다. 1) 각 균주들은 각각 그들의 특성을 가지고 있었으며 이로서 균동정의 가능성을 나타내었다. 2)Amilase측정결과에서 보면 비교적 역가가 높은 균주들이 관찰되었으며, 이는 일주문의 경과에 따라 역가가 증가하였으나 protease의 역가는 우수한 균주를 발견키가 어려웠다. 3)Iodine의 착색대와 비착색대의 비율에 의한 역가의 정성적인 측정이 가능하였다.

• 한국미생물·생명공학회지
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• 제23권1호
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• pp.91-97
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• 1995

The 543 microbial strains were isolated from the rhizosphere in ginseng root rot-suppressive soil. From these, isolate KGM-100 was finally selected for the screening of powerful antagonistic bacterium for the soil-born Fusarium solani causing root rot of many important crops. The isolate KGM-100 was identified as Pseudomonas aeruginosa. Antibiotics produced by the P. aeruginosa KGM-100 were partially characterized as heat-stable and low-molecular weight antibiotics. The strain also produced siderophore, which was assumed to be pseudobactin. Pot test showed approximately 30-50% biological control effects when Fusarium solani-infected soil was treated with the P. aeruginosa KGM-100.

• Journal of Microbiology
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• 제33권4호
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• pp.334-338
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• 1995

The electrophoretic karyotypes of 6 species in different Fusarium sections were examined by using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Intact chromosomal DNA was prepared from protoplasts and up to 9 distinct bands were separated on 0.7% or 0.8% agarose gel under several different conditions. Putative chromosome numbers varied from 6 to 9 amd polymorphic karyotypes were observed in different Fusarium sections. Using Schizosaccharomyces pombe and Saccharomyces cerevisiae chromosomes as standards, the sizes of the Fusarium spp. chromosomes were estimated. The electrophoretic karyotypes of F. moniliforme and F. subglutinans (section Liseola) were similar. Unidentified filamentour fungi, F. beomiforme was much closer to F. axysporum (section Elecgans) in karyotype and the karyotypes of F. napiforme were more similar to those of section Liseola than any other sections. F. graminearum (section Discolor) had a distinctive electrophoretic karyotype.

• Mycobiology
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• 제35권3호
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• pp.162-165
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• 2007

Thirty seven species of Fusarium were evaluated for their ability of producing extracellular enzymes using chromogenic medium containing substrates such as starch, cellobiose, CM-cellulose, xylan, and pectin. Among the tested species Fusarium mesoamericanum, F. graminearum, F. asiaticum, and F. acuminatum showed high ${\beta}$-glucosidase acitivity. Xylanase activity was strongly detected in F. proliferatum and F. oxysporum. Strong pectinase activity was also found in F. oxysporum and F. proliferatum. Amylase activity was apparent in F. oxysporum. No clear activity in cellulase was found from all the Fusarium species tested.

• The Plant Pathology Journal
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• 제28권3호
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• pp.311-316
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• 2012

Fusarium root disease can be a serious problem in forest and conservation nurseries in the western United States. Fusarium inoculum is commonly found in most container and bareroot nurseries on healthy and diseased seedlings, in nursery soils, and on conifer seeds. Fusarium spp. within the F. oxysporum species complex have been recognized as pathogens for more than a century, but attempts to distinguish virulence by correlating morphological characteristics with results of pathogenicity tests were unsuccessful. Recent molecular characterization and pathogenicity tests, however, revealed that selected isolates of F. oxysporum are benign on Douglas-fir (Pseudotsuga menziesii) seedlings. Other morphologically indistinguishable isolates, which can be virulent, were identified as F. commune, a recently described species. In a replicated greenhouse study, inoculating Douglas-fir seedlings with one isolate of F. oxysporum prevented expression of disease caused by a virulent isolate of F. commune. Moreover, seedling survival and growth was unaffected by the presence of the F. oxysporum isolate, and this isolate yielded better biological control than a commercial formulation of Bacillus subtilis. These results demonstrate that an isolate of nonpathogenic F. oxysporum can effectively reduce Fusarium root disease of Douglas-fir caused by F. commune under nursery settings, and this biological control approach has potential for further development.

• 원예과학기술지
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• 제16권3호
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• pp.347-349
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• 1998

본 실험은 국내 농가에서 가장 적용하기 간편하고 효율적인 모래여과(slow sand filtration)방법을 기초로 하였으며, 여러 가지 여과용 매질을 적용하여 병원균 제거 효율을 검정하고자 하였다. 양액 1,500 liter 여과후, 매질별 균 제거효율은 Fusarium oxysporum을 기준으로 볼 때, 활성탄 92.5%, 석영모래 90.8%, 버미큘라이트 90.5%, 강모래 82.3%, 펄라이트 50.4%, 하이드로볼 21.2% 순으로 나타났고, 균주별 시험의 경우, 석영모래를 기준으로 여과액에서 검정되는 최대 농도가 Fusarium oxysporum 120 cfu/mL. Collectotrichum lagenarium 98 cfu/mL. Phytophthora capsici 82 cfu/mL, Botrytis cinerea 62 cfu/mL, Pythium spp. 42 cfu/mL, Sclerotinia spp. 52 cfu/mL로 나타났다. 여과후, pH 및 EC의 변화는 석영모래를 기준으로 볼 때, pH는 약 7로유지되었고, EC 는 큰 차이가 없었다. '뚝섬 적축면' 상추와 '서광' 토마토를 담액수경으로 재배하며, 석영모래, 활성탄, 버미큘라이트를 충진물로 한 여과시스템을 적용한 결과, 균을 인위접종 후 10주 후까지 발병하지 않아, 본 여과시스템으로 효과적인 균제거가 가능함을 보여주었다. 실제 농가수준에서도 본 시스템을 적용할 경우, 균 제거능 및 경제성에 있어 효율적이라 사료된다.

• The Plant Pathology Journal
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• 제18권5호
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• pp.293-299
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• 2002

A survey of Fusarium wilt of perilla was conducted in 12 locations in Korea from 1999 to 2001. The disease occurred in 74 out of 187 fields in the 12 locations surveyed, and incidence of the disease reached up to 30% at its maximum in some perilla fields in Seosan and Dangjin. Incidence of the disease in the other locations ranged from 0.2 to 20%. A total of 327 isolates of Fusarium spp. were obtained from stems and roots of the diseased perilla plants. The isolates were identified based on their morphological characteristics. Out of the 327 isolates of Fusarium, 277 isolates from 12 locations were identified as F. oxysporum, 11 isolates from three locations as F. solani,17 isolates from two locations as F. equiseti, 4 isolates from one location as F. avenaceum and 6 isolates from one location as F. subglutinans. The other 12 isolates of Fusarium from four locations were unidentified. Twelve isolates of F. oxysporum and two isolates each of the other Fusarium spp. were tested for their pathogenicity to five cultivars of perilla. Seven isolates of F. oxysporum were strongly pathogenic to some perilla cultivars, but the other five isolates were weakly or not pathogenic. One isolate of F. solani was strongly pathogenic to all the perilla cultivars tested, but another isolate was not pathogenic. All the isolates of F. equiseti, F. avenaceum, and F. Subglutinans tested were not pathogenic to any of the perilla cultivars tested. Symptoms on the perilla plants induced by artificial inoculation with strongly pathogenic isolates of F. oxysporum and F. solani appeared as wilt, stem blight, and root yet, which were similar to those observed in the fields. The isolates which induced symptoms by artificial inoculation were re-isolated from the lesions of the perilla plants inoculated. All the isolates of F. oxysporum tested were not pathogenic to eight other crops inoculated. Results of this study reveal that F. oxysporum is the main pathogen of perilla wilt and that it is host specific to perilla. forma specialis of F. oxysporum causing wilt of perilla is proposed as perillae.

• Mycobiology
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• 제42권2호
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• pp.158-166
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• 2014

In this study, bacterial strains were isolated from soils from 30 locations of Samcheok, Gangwon province. Of the isolated strains, seven showed potential plant growth promoting and antagonistic activities. Based on cultural and morphological characterization, and 16S rRNA gene sequencing, these strains were identified as Paenibacillus species. All seven strains produced ammonia, cellulase, hydrocyanic acid, indole-3-acetic acid, protease, phosphatase, and siderophores. They also inhibited the mycelial growth of Fusarium oxysporum f. sp. radicis-lycopersici in vitro. The seven Paenibacillus strains enhanced a range of growth parameters in tomato plants under greenhouse conditions, in comparison with non-inoculated control plants. Notably, treatment of tomato plants with one identified strain, P. polymyxa SC09-21, resulted in 80.0% suppression of fusarium crown and root rot under greenhouse conditions. The plant growth promoting and antifungal activity of P. polymyxa SC09-21 identified in this study highlight its potential suitability as a bioinoculant.

• 한국응용곤충학회지
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• 제11권2호
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• pp.109-111
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• 1972

종자 산균제로써 Dithane M-45는 종래 사용해오던 유기수은제 보다 약간 우세한 살균력을 나타냈으며 적정농도는 1000ppm이였고, 적정시간은 4-6시간이었다. 그러나 Fusarium spp.에 대해서는 어느 농도에서도 흡족할만한 살균력을 나타내지 못하였다.

• 미생물학회지
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• 제12권2호
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• pp.94-98
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• 1974

This study was conducted from April 1 to September 31, 1973. Ten strains of Fusarium spp. were isolated from the diseased ginseng in two local areas at Kangwha-Gun nad Kumsan-Gun in Korea. Among of them, 2 strains ($G_1$, $G_4$) did not have virulence to ginseng in reinoculation. Their cultural, morphological characteristic and hose virulence to pea seedling were examined. Taxonomical identification of 8 isolates followed by the method of Wollenweber, Snyder nad Toussoun, Booth, Matuo and Snyder. All of eight strains were identified as the Fusarium solani f.sp.pisi(Jones) Synd. et Hans.