• 농약과학회지
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• 제4권2호
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• pp.1-10
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• 2000

국내 살균제 저항성 연구는 아직 초보적 수준으로 대부분의 연구가 실내에서의 살균제 저항성 균주의 검출보고에 국한하고 있다. 향후 살균제 저항성연구는 포장에서 대상 병원균 집단의 살균제에 대한 감수성 분포를 근거로 하여 그 살균제에 대한 저항성을 판별하는 기준 농도를 설정한 후 병원균주의 저항성 여부가 판정되어야 한다. 국내의 대부분의 연구가 이점을 간과하고 있기 때문에 연구자간에 저항성 기준에 차이가 있어 병원균 집단의 약제에 대한 감수성 변화를 추적하거나 해석하는데 많은 문제점이 있었다. 실내시험에서 검출된 살균제 저항성은 포장에서 감수성 및 저항성 균을 대상으로 방제효과를 조사한 후 그 저항성을 실증할 수 있어야 한다. 살균제 저항성은 실제로 농가포장에서의 약재 방제효율의 저하와 연관되어야 비로서 실용적인 의미를 갖기 때문이다. 저항성 균주에 대한 기생적 적응력의 조사는 병원균집단내의 저항성 균주들의 생존력이나 안정성을 검정하기 위하여 반드시 필요하다. 또한 살균제 저항성에 대한 장.단기적 대책을 강구하기 위하여 병원균 집단의 특정 살균제에 대한 감수성의 변화가 그 살균제를 사용하고 있거나 사용한 적이 없는 포장에서 주의 깊게 조사되어야만 한다. 이러한 연구들은 국내 살균제 저항성 문제를 해결하기 위한 기본적인 정보를 제공한다는 면에서 매우 시급한 연구과제가 되고 있다.

• 식물병연구
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• 제14권1호
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• pp.26-31
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• 2008

구기자 탄저병 방제를 위한 살균제를 선발하기 위하여 13개의 탄저병 균주는 구기자의 청양재래 품종에 대해서 숙과와 미숙과 모두에서 상처 접종 시 병원성이 강하게 나타났다. 또한 15종 살균제의 균사생육 억제 효과를 실내 검정하여 탄저병균에 대한 균사 생장 억제 효과가 우수한 dithianon WG, tebuconazole SC, tebuconazole WG, trifloxystrobin+tebuconazole SC, azoxystrobin SC 및 polyoxin D zinc salt+carbendazim WP 등 6종의 살균제를 선발하였다. 선발한 살균제들의 포장에서 병 방제 효과를 조사한 결과 dithianon WG, tebuconazole WG, trifloxystrobin+tebuconazole SC, tebuconazole SC 및 azoxystrobin SC 등 5종의 살균제가 높은 방제효과를 나타냈다.

• 한국약용작물학회지
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• 제23권6호
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• pp.473-479
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• 2015

Background : To control ginseng gray mold, farmers have mainly used inorganic chemical based fungicides. The recent emergence of fungicide resistance has reduced the effectiveness of such control methods. Such pesticides also carry additional problems, such as diffuse pollution. Methods and Results : Six treatments of organic agricultural materials were tested for control of ginseng gray mold, CAPW (Chrysophanic acid + Phytoncide + Wood vinegar), EmEWV (Emodin + Ethanol + Wood vinegar), CEWV (Curcumin + Eugenol + Wood vinegar), Bacillus subtilis, soybean oil and sulfur. The control effect for gray mold by a single application of the agrochemical fungicide industrial Fenhexamid wettable powder (WP) was 84.4%. The control effect by CAPW, EmEWV and CEWV varied between 52.7 - 64.9%. The control effect by B. subtilis, soybean oil, and sulfur were 32.9 - 59.2%. Conclusions : In the field tests, CAPW showed the highest control effects when used before, and at first stage of disease incidence, against ginseng gray mold.

• The Plant Pathology Journal
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• 제30권3호
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• pp.279-287
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• 2014

KYC 3262 was selected as a biocontrol agent against anthracnose on hot pepper from 813 extracts of myxobacterial isolates. Dual culture with Colletotrichum acutatum and 813 myxobacterial extracts was conducted, and 19 extracts were selected that inhibited germination and mycelial growth of C. acutatum. All selections were Sorangium cellulosum, which are cellulolytic myxobacteria from soil. With the infection bioassay on detached fruits in airtight containers, KYC 3262, KYC 3512, KYC 3279, and KYC 3584 were selected. The listed four myxobacteria were cultured in CSG/1 liquid media, and harvested filtrates were sprayed on the infected fruits. KYC 3262 was selected from the studies of attached fruit in a greenhouse study. KYC 3262 filtrate was applied for 3 years (from 2011 to 2013) in a field study in Asan, Republic of Korea. Control values of the KYC 3262 in the field were 31%, 89%, and 82% in 2011, 2012, and 2013, whereas values of the fungicide spray treatment were 19%, 97%, and 91%, respectively. Yields (kg/20 plants) of the KYC 3262 were 2.66 kg and 18.6 kg in 2011 and 2013, respectively, and those of the fungicide treatment were 2.0 kg and 20.2 kg, in 2011 and 2013, respectively.

• The Plant Pathology Journal
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• 제27권1호
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• pp.59-67
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• 2011

A bacterial strain CH-67 which exhibits antagonism towards several plant pathogenic fungi such as Botrytis cinerea, Fulvia fulva, Rhizoctonia solani, Sclerotinia sclerotiorum, Colletotrichum sp. and Phytophthora sp. was isolated from forest soil by a chitin-baiting method. This strain was identified as Burkholderia cepacia complex (Bcc) and belonging to genomovar IX (Burkholderia pyrrocinia) by colony morphology, biochemical traits and molecular method like 16S rRNA and recA gene analysis. This strain was used to develop a bio-fungicide for the control of tomato leaf mold caused by Fulvia fulva. Various formulations of B. pyrrocinia CH-67 were prepared using fermentation cultures of the bacterium in rice oil medium. The result of pot experiments led to selection of the wettable powder formulation CH67-C containing modified starch as the best formulation for the control of tomato leaf mold. CH67-C, at 100-fold dilution, showed a control value of 85% against tomato leaf mold. Its disease control efficacy was not significantly different from that of the chemical fungicide triflumidazole. B. pyrrocinia CH-67 was also effective in controlling damping-off caused by Rhizoctonia solani PY-1 in crisphead lettuce and tomato plants. CH67-C formulation was recognized as a cell-free formulation since B. pyrrocinia CH-67 was all lethal during formulation process. This study provides an effective biocontrol formulation of biofungicide using B. pyrrocinia CH-67 to control tomato leaf mold and damping-off crisphead lettuce and tomato.

• 한국약용작물학회지
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• 제21권5호
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• pp.388-393
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• 2013

This study was conducted to select environmental friendly organic materials for controlling the ginseng alternaria blight and to evaluate their effects from 2011 to 2012. Alternaria blight is caused by Alternaria panax and is the most common ginseng disease in Korea. Environmental friendly organic materials were used to reduce amount of chemical fungicides and the number of spray for control of Ginseng Alternaria leaf blight. In 4 years of ginseng, control value of Alternaria leaf blight by single application of Defenoconazole WP was 82.3% and those of single application was 62.0~75.9%. Consequently, mixed or alternated application of eco-material products could be recommended as a control method to reduce the amount of fungicides.

• International Journal of Industrial Entomology and Biomaterials
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• 제14권2호
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• pp.143-146
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• 2007

Concerns of entomopathogenic fungi as alternative pest control agents are increasing even though chemical pesticides have been used as the main control agents for pests and diseases in crop production. This study was conducted to test the influence of fungicides and insecticides on an isolate of Lecanicillium attenuatum that was reported to have the pathogenicity against cotton aphid, because fungicides and/or insecticides can apply with mycopesticides simultaneous, before and/or after. Fungicides fenbuconazole+thiram and propineb inhibited the spore germination and mycelial growth of L. attenuatum CS625; dimethomorph and procymidone did not affect spore germination or mycelial growth. The insecticide abamectin, deltamethrin, imidachropride, and spinosad had no detrimental effects on spore germination or mycelial growth. Therefore, these results demonstrated that careful selection of pesticides and fungicides can be applied to the integrated pest and disease control with microbial pesticide.

• 한국양봉학회지
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• 제32권4호
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• pp.295-302
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• 2017

Avoidance behavior is an important life history strategy to survive hazardous environment. The experiment was conducted to detect the avoidance tendency of the honeybee Apis mellifera against commonly used pesticides in apple production. Choice test given only 50% sucrose solution and pesticide-mixed sucrose solution as food estimated the avoidance in laboratory. Most of the acaricides and fungicides tested were shown avoided. Among insecticides, honeybee showed strong avoidance to cyhexatine, carbosulfan and fenpyroximate but low to diflubenzuron, tebufenpyrad, and acrinathrin. Avoidance behavior to neonicotinoid insecticides showed bifurcated; highly avoided from thiacloprid, acetamiprid while less avoided from imidacloprid, thiamethoxam and dinotefuran. From the field study, abamectin, fenthion, amitraz and acequinocyl showed highly avoided while fungicide of fenarimol, acaricides of acrinathrin and phosphamidon, IGR insecticide of diflubenzuron, neonicotinoid insecticide of imidacloprid, and carbamate insecticide of carbaryl showed less avoidance in the field. These results partly explained high bee poisoning from carbaryl in apple flowering period, and neonicotinoids during season.

• 원예과학기술지
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• 제29권2호
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• pp.151-155
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• 2011

Botrytis cinerea에 의한 딸기 잿빛곰팡이병은 최대 50%까지 발생하여 큰 피해를 주는 딸기의 주요 병해이다. 약제살포시기는 잿빛곰팡이병의 성공적인 방제를 좌우하는 필수적 요소이다. 딸기 '설향' 품종에 대한 잿빛곰팡이병 방제효과 검정을 위해 fenhexamid + imonoctadine tris, cyprodinil + fludioxonil, Bacillus subtilis QST713을 ex vivo에서 개화전, 개화기, 미착색과, 성숙과 단계부터 처리하여 효과를 비교하였다. 또한 '설향' 품종을 공시하여 ex vivo와 재배포장에서 cyprodinil + fludioxonil 혹은 fludioxonil을 저온($0^{\circ}C$, 5시간) 전후 처리하여 잿빛곰팡이병 방제효과를 조사하였다. 딸기 꽃과 성숙과실은 미착색과실보다 B. cinerea에 더 감수성이었다. ex vivo에서 개화단계별 약제방제효과는 fenhexamid + iminoctadine tris는 개화직전과 개화기, cyprodinil + fludioxonil은 개화기에 효과적이었으나 Bacillus subtilis QST713은 처리간 유의성이 없었다. 2010년 딸기 잿빛곰팡이병 방제효과는 fludioxonil을 개화기에 1주일 간격으로 2회 처리시 효과적이었다. 딸기 'Redpearl'과 '설향' 품종은 '매향'과 'Akihime' 품종보다 저온에 더 감수성이어서 주의를 요했으며 cyprodinil + fludioxonil을 저온처리 전에 살포시 90%이상의 방제효과를 나타냈다. 2010년 재배포장에서 잿빛곰팡이병 방제효과는 저온 발생 전 혹은 저온 발생 후 1주일 간격 2-3회 fludioxonil처리가 효과적이었다. 이런 결과로 딸기 잿빛곰팡이병 방제를 위한 약제의 효과적인 처리시기는 개화와 저온이 밀접한 관련이 있음을 알 수 있었다.

• 한국작물학회지
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• 제35권1호
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• pp.97-107
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• 1990

The epidemiology of plant disease deals with the dynamic processes of host-pathogen interactions, which determine the prevalence and severity of the disease. Epidemic processes for most foliar diseases of plants follow a series of steps: arrival of pathogens on plant surfaces, initial infection, incubation period, latent period, sporulation, dissemination of secondary inoculum, and infectious period. These complex biological processes are influenced by the environment-Man also often interfers with these processes by altering the host and pathogen populations and the environment. Slowing or halting any of the epidemic processes can delay the development of the epidemic, so that serious losses in yield due to disease do not occur. It is generally recognized that the most effective and efficient method of minimizing disease damage is through the use of resistant cultivars, particularly when other methods such as fungicide applications are not economically feasible-Populations of plant pathogens are not genetically uniform nor are they necessarily stable. Cultivars bred for resistance to current populations of a pathogen may not be resistant in the future due to selection pressures placed on the pathogen populations. Understanding population development and genetic variability in the pathogen, and knowledge of the genetics of resistance in the plant should help in developing breeding strategies that wi1l provide effective and stable disease control through genetic resistance. In the United States, soybeans have ranked first in value of crops sold off the farm in recent years. Soybeans have been the leading U. S.