• Journal of Microbiology and Biotechnology
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• v.13 no.1
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• pp.57-63
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• 2003

Using fungal (Fusarium solani f. pisi) and bacterial (Pseudomonas mendocina) cutinases, the initial hydrolysis rate of p-nitrophenyl esters was systematically estimated for a wide range of enzyme and substrate concentrations using a 96-well microplate reader. Both cutinases exhibited a high substrate specificity; i.e. a high hydrolytic activity on p-nitrophenyl butyrate (PNB), yet extremely low activity on p-nitrophenyl palmitate (PNP). When compared to the hydrolysis of PNB and PNP by other hydrolases [lipases and esterases derived from different microbial sources, such as bacteria (Pseudomonas cepacia, Psedomonas furescens, Baciilus stearothermophilus), molds (Aspeillus niger, mucor miehei), and yeasts (Candida rugosa, Candida cylindracea)], the above substrate specificity would seem to be a unique characteristic of cutinases. Secondly, the hydrolytic activity of the cutinases on PNB appeared much faster than that of the other hydrolytic enzymes mentioned above. Furthermore, the current study proved that even when the cutinases were mixed with large amounts of other hydrolases (lipases or esterases), the Initial hydrolysis rate of PNB was determined only by the cutinase concentration for each PNB concentration. This property of cutinase activity would seem to result from a higher accessibility to the substrate PNB, compared with the other hydrolytic enzymes. Accordingly, these distinct properties of cutinases may be very useful in the rapid and easy isolation of various natural cutinases with different microbial sources, each of which may provide a novel industrial application with a specific enzymatic function.

• Microbiology and Biotechnology Letters
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• v.25 no.4
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• pp.346-353
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• 1997

During the screening of cellulase producing microorganisms, a fungal strain C-4 was selected from etiolated leaves. Based on taxonomic studies, the fungus could be classified as a strain of Trichoderma sp. When the strain C-4 was cultured in Mandels' media at 28$circ$C for 6 days, the enzyme activities detected in broth were as follows: 8.2 U/ml (28.1 U/mg) of CMCase activity, 0.75 U/ml (2.58 U/mg) of Avicelase activity, 1.67 U/ ml (5.68 U/mg) of $eta$-glucosidase activity. The optimum pH for enzyme induction was 6.2. The crude enzyme retained 100% of its original CMCase activity at 50$circ$C for 1 hr (pH 5.0), and at 4$circ$C for 24 hrs (pH 5.0). There was no effect on the CMCase activity in the presence of 1 mM of CsCl, LiCl, MgCl$_{2}$, and FeCl$_{2}$, respectively. When the crude enzyme was treated with trypsin and chymotrypsin (2% W/w) for 10 minutes, the remaining CMCase activity was 70%, but there was no further loss of activity for 60 minutes treatment at 30$circ$C. The crude enzyme showed the synergism with rumen fluid for the hydrolysis of Avicel and CMC by 118% and 130%, respectively.

• Journal of Microbiology and Biotechnology
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• v.20 no.7
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• pp.1114-1120
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• 2010

A chitosan-degrading fungus, BSF114, was isolated from soil. The culture preparation showed strong chitosanolytic enzyme activity at an optimum pH of 4.0 and optimum temperature of $60^{\circ}C$ after 36-40 h fermentation. The rapid decrease in the viscosity of the chitosan solution early in the reaction suggested an endo-type cleavage of the polymeric chitosan chains. To identify the isolated fungus, molecular biological and morphological methods were used. The fungal internal transcribed spacer (ITS) region 1 was amplified, sequenced, and then compared with related sequences in the GenBank database using BLAST. The phylogenetic relationships were then analyzed, and the results showed that the fungus belongs to Aspergillus fumigatus. Morphological observations were also used to confirm the above conclusion. The chitooligosaccharides (COS) obtained through hydrolyzing the colloidal chitosan showed that A. fumigatus BSF114 is suitable for degrading chitosan and producing chitooligosaccharides on a large scale. High concentrations of the COS (1,000 and 500 ${\mu}g/ml$) significantly proliferated mice marrow cells.

• Journal of Veterinary Clinics
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• v.34 no.5
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• pp.381-383
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• 2017

A two-year-old intact male Labrador retriever was presented with generalized erythema, pustule and pruritus. A skin screening test revealed that there were no fleas but bacteria and dermatophytes were present. Blood testing revealed no remarkable findings. The patient was prescribed systemic medication of enrofloxacin 30 mg/kg once a day and itraconazole 10 mg/kg once a day and topical medication of 2% chlorhexidine shampoo twice a week for 2 weeks. Two weeks after the prescription, aerobic culture confirmed that the bacteria were Leclercia Adecarboxylata and Pseudomonas putida was sensitive to enrofloxacin. Therefore, more medicine was prescribed for 4 weeks to alleviate clinical signs. After six weeks of medication, clinical signs were alleviated and skin screening test revealed no remarkable findings. Bacterial and fungal skin infections are common in dogs. However, there are no reports of Leclercia Adecarboxylata infection even in gastrointestinal tract in veterinary medicine. This is the first report of Leclercia Adecarboxylata infection in dogs. This report proved that Leclercia Adecarboxylata can cause skin problem in dogs.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.11b
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• pp.55-55
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• 2002

Occurrences of diseased sweet flag (Acorus calamus L. var. asiaticus) were found in Chonju and Buan Province, on August 2002. The typical symptoms of the disease affected the leaves, pods, and collar of the infected plants. The leaves or pods became darker brown, then dry rotted, and white fluffy mycelia formed on the lesion. The collar, of the infected plants, formed black spot. The spores grew rapidly on PDA medium. Pathogenic fungi have not been identified clearly, as of yet. These fungi were formed from developed spores, as well as, undeveloped spores. These fungi suggest that Fusarium sp. and Rhizoctonia sp.. The range of temperatures were tested from 5$^{\circ}C$ to 35$^{\circ}C$ for mycelial growth. The optimum temperature for growth was 3$0^{\circ}C$. This is the first report on the fungus disease of sweet flag by some pathogens, in Korea. We would like to do further research for single spore isolation, pathogenity, and characterization of fungi.

• Microbiology and Biotechnology Letters
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• v.24 no.1
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• pp.27-36
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• 1996

The aim of the present research program was to identify and develop strains of actinomycetes producing antifungal antibiotics which inhibit cell wall biosynthesis. 860 strains of Actinomycetes were isolated from various soil samples. Three isolates, EMS4, EMP22, and L234 were selected as the strains producing antifungal antibiotics inducing abnormal morphology against Penicillium cyclopium, Cryptococcus laurentii, and Aspergillus flavus, respectively. Taxonomic unit characters of the strains were tested and the data were analyzed numerically using TAXON program. EMS4, EMP22, and L234 were indentified to be a member of Streptomyces lavendulae, Streptomyces willmorei, and Streptomyces aburaviensis, respectively.

• Microbiology and Biotechnology Letters
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• v.31 no.1
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• pp.1-12
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• 2003

Myxobacteria are soil bacteria that move by gliding and have a complicated life cycle. In the research over the 25 years the myxobacteria have been shown to be a rich source of potentially useful bioactive substances. So far about 80 different basic compounds and 450 structural variants have been characterized. It is remarkable that myxobacteria produce the substance has special mechanisms. 26 new electron transport inhibitors,5 inhibitors of nucleic acid polymerases, 10 substances that act on the cytoskeleton, and 1 inhibitor of fungal acetyl-CoA carboxylase have been found. Presently, large-scale technical process was not fully established. But one of the compounds from myxobacteria is able to pass the many thresholds, which are on the road to application.

• YAKHAK HOEJI
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• v.52 no.6
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• pp.494-499
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• 2008

The role of antibody in the fungal infections is controversial. However, our previous reports showed a certain epitope in Candida albicans cell wall (CACW) induces protective antibody. A major problem is that the epitope isolation requires tremendous time with high cost. This aspect led us to investigate a simple way inducing protective antibodies against C. albicans. In the present study, we determined if $18{\beta}$-glycyrrhetinic acid ($18{\beta}$-GA) from Glabrae Radix (a family of Leguminosae) has immunoadjuvant activity. Data displayed that the $18{\beta}$-GA suppressed proliferations of both T- and Blymphocytes at high concentrations, whereas below 20 ${\mu}M$ concentration the compound supported the proliferations. These observations indicate that $18{\beta}$-GA has immunoregulatory activity. Based on this observation, an immunoadjuvant effect was examined at the low concentration. Results from animal experiments showed that CACW combined with or without $18{\beta}$-GA produced the anti-C. albicans antiserum in mice. Nevertheless, the CACW combined with $18{\beta}$-GA formula only protected mice against disseminated candidiasis (P<0.05). These data implicate that $18{\beta}$-GA has immunoadjuvant activity, which may provoke the CACW antigen to induce protective antibody. Currently, we are investigating possible mechanism of how the $18{\beta}$-GA provokes such protective immunity against the disseminated disease.

• Journal of Life Science
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• v.9 no.3
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• pp.314-327
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• 1999

This study was investigated to isolate identify the total bacteria and fungi from the indoor air of work-place of the shoes, paint, stainless steel, and plastic industries. The number of bacterial colonies on the nutrient agar plates were calculated by the open petridish method for 30 minutes in indoor air of work-places at the autumn and winter. The isolated bacteria were identified by Gram stain and biochemical test using API Staph and API 20E kits. The isolated fungal colonies were identified by gross appearance of the giant colonies and microscopic examination of their spore and hyphal characteristics on the slide culture method. The minimum inhibitory concentration (MIC) of several antibiotics against isolated bacteria was determined by the microdilution method with Mueller-Hinton broth. The 70-400 colonies in autumn and 54-236 colonies in winter were isolated from the indoor air of work-places of several industry. The isolation rates of Gram positive cocci, Gram positive bacilli, Gram negative bacilli, and Gram negative cocci were 46.3%, 19.8%, 17.3%, and 16.1%, respectively. In Gram positive cocci, the most strains were identified as Aerococcus spp, Micrococcus spp, and Staphylococcus spp. In Gram positive and negative bacilli, and Gram negative cocci were identified as Bacillus spp, Pseudomonas spp, and Neisseria spp, respectively. The frequently isolated fungi were Aspergillus spp, Penicillium spp and Rhizopus spp, respectively. The frequently isolated Aerococcus spp, Micrococcus spp, and Staphylococus spp were highly resistance against ampicillin, erythromycin, methicillin, and tetracycline. These results arouse our attention to microbiologic pollution in the indoor air of work-places of industries.

• The Korean Journal of Food & Health Convergence
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• v.6 no.3
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• pp.5-21
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• 2020

Bungkang (Syzygium polyanthum) is a medium to tall plant which produces medicinal root-bark, the plant is normally found along inland river bank and produces small white flowers and fruits. Essential oils are among the most interesting components of the plant extracts consisting mostly of monoterpenoid or sesquiterpenoids. They are used as therapeutic agents in ethno, conventional, and complementary alternative medicines. Investigation and evaluation of the essential oil of Syzygium polyanthum as well as the antibacterial, antioxidant and antifungal activity was ascertained. The experiment was performed. 100 chemical constituents were obtained and two pure compound was isolated as Eugenol (1) and Farnesol (2). Significant growth inhibition of Staphylococcus aureus, (ATCCⓒ25923) Klebsiellia pneumonia (ATCCⓒ19155), Salmonella typhi (ATCCⓒ14028) and Escherichia coli (ATCC©25922) and the fungal strains Aspergillus flavin, Aspergillus niger, Candida, tropicalis, and Fusarium oxysporium was observed from the essential oil at concentration of 500 ㎍/mL. Antioxidant potential was observed to be strong of 18.42 ㎍/mL when compared to the control of 15.23 ㎍/mL. The result indicated that the oil obtained from root-bark of Syzygium polyanthum can be considered as an agent for antioxidant, antibacterial and antifungal in pharmaceutical food and cosmetic industries trails.