• Journal of Microbiology and Biotechnology
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• v.25 no.2
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• pp.152-161
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• 2015

CodY is a highly conserved protein in low G+C gram-positive bacteria that regulates genes involved in sporulation and stationary-phase adaptation. Bacillus thuringiensis is a grampositive bacterium that forms spores and parasporal crystals during the stationary phase. To our knowledge, the regulatory mechanism of CodY in B. thuringiensis is unknown. To study the function of CodY protein in B. thuringiensis, BMB171codY^- was constructed in a BMB171 strain. A shuttle vector containing the ORF of cry1Ac10 was transformed into BMB171 and BMB171codY^-, named BMB171cry1Ac and BMB171codY^-cry1Ac, respectively. Some morphological and physiological changes of codY mutant BMB171codY^-cry1Ac were observed. A comparative proteomic analysis was conducted for both BMB171codY^-cry1Ac and BMB171cry1Ac through two-dimensional gel electrophoresis and MALDI-TOF-MS/MS analysis. The results showed that the proteins regulated by CodY are involved in microbial metabolism, including branched-chain amino acid metabolism, carbohydrate metabolism, fatty acid metabolism, and energy metabolism. Furthermore, we found CodY to be involved in sporulation, biosynthesis of poly-β-hydroxybutyrate, growth, genetic competence, and translation. According to the analysis of differentially expressed proteins, and physiological characterization of the codY mutant, we performed bacterial one-hybrid and electrophoretic mobility shift assay experiments and confirmed the direct regulation of genes by CodY, specifically those involved in metabolism of branched-chain amino acids, ribosomal recycling factor FRR, and the late competence protein ComER. Our data establish the foundation for in-depth study of the regulation of CodY in B. thuringiensis, and also offer a potential biocatalyst for functions of CodY in other bacteria.

• Journal of the Korea Convergence Society
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• v.11 no.10
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• pp.187-195
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• 2020

The purpose of study is to find ways to improve online research work in the near future environment. Study methods and results are as follows. First, the basis of research was secured through theoretical consideration. Second, three types were derived by collecting examples. There are reward, execution and visualization. Third, comprehensive matrix and directions were derived through case analysis. According to the study, the most frequently used phase of AI technology were analysis, visualization, execution, casting, design, and matching, The direction of use of AI can be largely divided into work efficiency, human thought assistance, and changes in research methods. But the plan phase is generally scarce. Overall, the lack of in function is provided, including AI technology. At a time when research plans are becoming more important as users and products diversify.

• The Journal of the Acoustical Society of Korea
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• v.19 no.7
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• pp.59-65
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• 2000

This paper describes the analysis of vibration characteristics through the human body as the research for voice therapy and diagnosis. The oscillation signal is not external forces but the self-voice to be pronounced the vowels ('a', 'e', 'i', 'o', 'u'). The experiment system consists of microphones, accelerometers and amplifiers. The input data are stored by the computer. At the same time, the voice is stored by the microphone and the vibration signal of the human body is stored by accelerometer. The 63 points are appointed in head, neck, trunk of human body. The positions and number of times are changeable by the purpose. The analysis parameters are amplitude, phase, fundamental. frequency, formant and the correlation of vibration signal and voice is measured by coherence function. The results show that the vibration signals have characteristic vibration in the positions of human body.

• Journal of the Korean Society of Systems Engineering
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• v.11 no.2
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• pp.61-73
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• 2015

The early-phase naval ship design demands requirements synthesis rather than design synthesis, which conducts engineering design for several domains on a detailed level. Requirements synthesis focuses on creating a balanced set of required operational capabilities satisfying user's needs and concept of operations. Requirements are evolved from capability based languages to function based language by statistical exploration and engineering design which are derived in the following order: concept alternative, concept baseline, initial baseline and functional baseline. The early-phase naval ship design process can be divided into three passes: concept definition, concept exploration and concept development. Main activities and outcomes in each pass are shortly presented. Concept definition is the first important step that produces a concept baseline through extensive design space exploration promptly. Design space exploration applies a statistical approach to explore design trends of existing ships and produce feasible design range corresponding to concept alternative. It further helps naval systems engineers and operational researchers by inducing useful responses to user and stakeholders' questions at a sufficient degree of confidence and success in the very early ship design. The focus of this paper is on the flow of design space exploration, and its application to a high-speed patrol craft. The views expressed in this paper are those of the authors, and do not reflect the official policy or rule of the Navy.

• Nuclear Engineering and Technology
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• v.44 no.8
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• pp.919-928
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• 2012

The applications of computers and communication system and network technologies in nuclear power plants have expanded recently. This application of digital technologies to the instrumentation and control systems of nuclear power plants brings with it the cyber security concerns similar to other critical infrastructures. Cyber security risk assessments for digital instrumentation and control systems have become more crucial in the development of new systems and in the operation of existing systems. Although the instrumentation and control systems of nuclear power plants are similar to industrial control systems, the former have specifications that differ from the latter in terms of architecture and function, in order to satisfy nuclear safety requirements, which need different methods for the application of cyber security risk assessment. In this paper, the characteristics of nuclear power plant instrumentation and control systems are described, and the considerations needed when conducting cyber security risk assessments in accordance with the lifecycle process of instrumentation and control systems are discussed. For cyber security risk assessments of instrumentation and control systems, the activities and considerations necessary for assessments during the system design phase or component design and equipment supply phase are presented in the following 6 steps: 1) System Identification and Cyber Security Modeling, 2) Asset and Impact Analysis, 3) Threat Analysis, 4) Vulnerability Analysis, 5) Security Control Design, and 6) Penetration test. The results from an application of the method to a digital reactor protection system are described.

• Nuclear Engineering and Technology
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• v.20 no.4
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• pp.253-264
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• 1988

Our experience of neutron noise analysis in French-type 900 MWe pressurized water reactor (PWR) is presented. Neutron noise analysis is based on the technique of interpreting the signal fluctuations of ex-core detectors caused by core reactivity changes and neutron attenuation due to lateral core motion. It also provides advantages over deterministic dynamic-testing techniques because existing plant instrumentation can be utilized and normal operation of the plant is not disturbed. The data of this paper were obtained in the ULJIN unit 1 reactor during the start-up test period and the statistical descriptors, useful for our purpose, are power spectral density (PSD), coherence function (CF), and phase difference between detectors. It is found that core support barrel (CSB) motions induced by coolant flow forces and pressure pulsations in a reactor vessel were indentified around 8 Hz of frequency.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.28 no.10C
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• pp.942-948
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• 2003

In this paper, we analyze the performance of multiple trellis coded modulation applied to continuous phase frequency shift keying (MTCM/CPFSK) for the noncoherent receiver without channel state information (CSI) on the interleaved Rician fading channel. In this system, the squared cross-correlation between the received signal and a candidate signal is used as the branch metric of the Viterbi decoder. To obtain the bit error performance of this system, we analyze the approximated pairwise error probability (PEP) and the exact PEP. We also derive the equivalent normalized squared distance (ENSD) and compare it with the ENSD of the noncoherent receiver with perfect CSI. Simulation results are also provided to verify the theoretical performance analysis.

• Journal of The Korean Society of Integrative Medicine
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• v.10 no.1
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• pp.91-99
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• 2022

Purpose : The goal of this study is to examine the effect of robot assisted gait training (RAGT) on the kinematic factors (temporospatial gait parameters, gait cycle ratio, and gait line length) of gait in stroke patients. Methods : The subjects of this study were 24 stroke patients selected by inclusion criteria. Participants were randomly allocated to two groups: robot assisted gait training (n=11) and general neurological physical therapy group (n=11). In the robot-assisted gait training group, robot-assisted gait training was mediated for 30 minutes a day in addition to general neurological physical therapy. The general neurological physical therapy group was mediated by general neurological physical therapy for 30 minutes a day in addition to general neurological physical therapy. The number of interventions was 5 times a week for 5 weeks. In order to compare the kinematic factors of walking between the two groups, gait analysis was performed before and after 5 weeks of training using the Zebris gait analysis system. Results : As a result of the gait analysis of the two groups, there were significant differences in temporospatial gait variables (step length, stride length, step width, step time, stride time), gait cycle ratio (swing phase, stance phase) and gait line length. However, there was no significant difference in the cadence (temporospatial gait parameters) in the robot assisted gait training group compared to general neurological physical therapy group. Conclusion : It is considered to be a useful treatment for stroke patients to promote the recovery of gait function in stroke patients. Based on the results of this study, continuous robot assisted gait training treatment is considered to have a positive effect on gait ability, the goal of stroke rehabilitation. In the future, additional studies should be conducted on many subjects of stroke patients, the kinematic factors of the legs according to the severity of stroke and treatment period, and the effect of gait training.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2007.06a
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• pp.758-761
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• 2007

Digital signal processing technique is applied in wide fields such as speech processing, image processing and spectrum analysis. Therefore, in order to do frequency selective operation digital filter is used in stead of analog filter and sharp filter characteristics can be implemented. Since finite impulse response (FIR) digital filter as nonrecursive type represents linear phase response characteristics and is always stable and is used in fields regarding wave information importantly such as data transmission. And due to frequency characteristics, in order to remove the Gibbs phenomenon generating around a discontinuous point, filter is designed through window function method. Therefore, in this paper to improve performance of FIR digital filter, a modified window function was applied. And the proposed method was compared with conventional methods using peak side-lobe and transition properties in simulations.

• Journal of the Institute of Convergence Signal Processing
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• v.13 no.4
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• pp.194-200
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• 2012

In this paper we analyze the code sequence generating algorism defined on $GF(2^n)$ proposed by S.Bostas and V.Kumar[7] and derive the implementation functions of code sequence generator using Boolean functions which can map the vector space $F_2^n$ of all binary vectors of length n, to the finite field with two elements $F_2$. We find the code sequence generating boolean functions based on two kinds of the primitive polynomials of degree, n=5 and n=7 from trace function. We then design and implement the code sequence generators using these functions, and produce two code sequence groups. The two groups have the period 31 and 127 and the magnitudes of out of phase(${\tau}{\neq}0$) autocorrelation and crosscorrelation functions {-9, -1, 7} and {-17, -1, 15}, satisfying the period $L=2^n-1$ and the correlation functions $R_{ij}({\tau})=\{-2^{(n+1)/2}-1,-1,2^{(n+l)/2}-1\}$ respectively. Through these results, we confirm that the code sequence generators using boolean functions are designed and implemented correctly.