• Title/Summary/Keyword: Full-field optical coherence tomography

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Scientific and Engineering Applications of Full-field Swept-source Optical Coherence Tomography

  • Mehta, Dalip Singh;Anna, Tulsi;Shakher, Chandra
    • Journal of the Optical Society of Korea
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    • v.13 no.3
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    • pp.341-348
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    • 2009
  • We report the development of full-field swept-source optical coherence tomography (SS-OCT) in the wavelength range of 815-870 nm using a unique combination of super-luminescent diode (SLD) as broad-band light source and acousto-optic tunable filter (AOTF) as a frequency-scanning device. Some new applications of full-field SS-OCT in forensic sciences and engineering materials have been demonstrated. Results of simultaneous topography and tomography of latent fingerprints, silicon microelectronic circuits and composite materials are presented. The main advantages of the present system are completely non-mechanical scanning, wide-field, compact and low-cost.

Counterfeit Detection Using Characterization of Safety Feature on Banknote with Full-field Optical Coherence Tomography

  • Choi, Woo-June;Min, Gi-Hyeon;Lee, Byeong-Ha;Eom, Jong-Hyun;Kim, Ju-Wan
    • Journal of the Optical Society of Korea
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    • v.14 no.4
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    • pp.316-320
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    • 2010
  • We report an application of full-field optical coherence tomography (FF-OCT) for identifying counterfeit bank notes. The depth-resolved imaging capability of FF-OCT was used for tomographic identification of superficially-identical objects. By retrieving the internal structures of the security feature (cash hologram) of an original banknote, we could demonstrate the feasibility of FF-OCT to identify counterfeit money. The FF-OCT images showed that the hologram consisted of micron scale multi-coated layers including an air gap. Therefore, it is expected that FF-OCT has potential as a new non-invasive tool to discern imitation of currency, and it would find applications in a wide field of counterfeit sciences.

Common-path Optical Interferometry for Stabilized Dynamic Contrast Imaging: A Feasibility Study

  • Seung-Jin, Lee;Young-Wan, Choi;Woo June, Choi
    • Current Optics and Photonics
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    • v.7 no.1
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    • pp.65-72
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    • 2023
  • The motion of organelles inside a cell is an important intrinsic indicator for assessing cell physiology and tissue viability. Dynamic contrast full-field optical coherence tomography (D-FFOCT) is a promising imaging technology that can visualize intracellular movements using the variance of temporal interference signals caused by biological motions. However, double-path interferometry in D-FFOCT can be highly vulnerable to surrounding noise, which may cause turbulence in the interference signals, contaminating the sample dynamics. Therefore, we propose a method for stabilized D-FFOCT imaging in noisy environments by using common-path interferometry in D-FFOCT. A comparative study shows that D-FFOCT with the proposed method achieves stable dynamic contrast imaging of a scattering phantom in motion that is over tenfold more noise-insensitive compared to the conventional one, and thus this imaging capability can provide cleaner motion contrast images. With the proposed approach, the intracellular dynamics of biological samples are imaged and monitored.

Identification of Fungus-infected Tomato Seeds Based on Full-Field Optical Coherence Tomography

  • Bharti, Bharti;Yoon, Taeil;Lee, Byeong Ha
    • Current Optics and Photonics
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    • v.3 no.6
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    • pp.571-576
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    • 2019
  • The morphological changes of anthracnose (fungus) -infected tomato seeds have been studied to identify the infection and characterize its effect. Full-field optical coherence tomography (FF-OCT) has been utilized as a nondestructive but efficient modality for visualizing the effects of fungal infection. The cross-sectional images extracted from a stack of en face FF-OCT images showed significant changes with infection in the seed structure. First of all, the seed coat disappeared with the infection. The thickness of the seed coat of a healthy seed was measured as 28.2 ㎛, with a standard deviation of 1.2 ㎛. However, for infected seeds the gap between surface and endosperm was not appreciably observed. In addition, the measurements confirmed that the dryness of seeds did not affect the internal seed structure. The reconstructed three-dimensional (3D) image revealed that the permeability of the seed coat, which plays the vital role of protecting the seed, is also affected by the infection. These results suggest that FF-OCT has good potential for the identification of fungus-infected tomato seeds, and for many other tasks in agriculture.

Realization of 3-D Topographic and Tomograpic Images with Ultrahigh-resolution Full-field Optical Coherence Tomography

  • Choi, Woo-June;Na, Ji-Hoon;Ryu, Seon-Young;Lee, Byeong-Ha;Ko, Dong-Seob
    • Journal of the Optical Society of Korea
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    • v.11 no.1
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    • pp.18-25
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    • 2007
  • We present an ultrahigh-resolution full-field optical coherence tomography (FF-OCT) implemented with a white-light interference microscope and a detector array as an alternative OCT technique. The use of detector array allows the capture of two-dimensional en-face images in parallel without taking any lateral scanning process. The phase shifting interferometric technique with the sinusoidal phase modulation (SPM) is utilized to get the demodulated OCT images. The configuration of the system and the resolution of the obtained image are presented. The topographic images, taken with the implemented system, of a coin, an integrated circuit chip, and the tomographic images of an onion epithelium are demonstrated also. Axial and lateral spatial resolution of ${\sim}1.0{\mu}m$ and ${\sim}2.0{\mu}m$ are achieved with the system respectively.

Assessment of the pigeon (Columba livia) retina with spectral domain optical coherence tomography

  • Kim, Sunhyo;Kang, Seonmi;Susanti, Lina;Seo, Kangmoon
    • Journal of Veterinary Science
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    • v.22 no.5
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    • pp.65.1-65.12
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    • 2021
  • Background: To assess the normal retina of the pigeon eye using spectral domain optical coherence tomography (SD-OCT) and establish a normative reference. Methods: Twelve eyes of six ophthalmologically normal pigeons (Columba livia) were included. SD-OCT images were taken with dilated pupils under sedation. Four meridians, including the fovea, optic disc, red field, and yellow field, were obtained in each eye. The layers, including full thickness (FT), ganglion cell complex (GCC), thickness from the retinal pigmented epithelium to the outer nuclear layer (RPE-ONL), and from the retinal pigmented epithelium to the inner nuclear layer (RPE-INL), were manually measured. Results: The average FT values were significantly different among the four meridians (p < 0.05), with the optic disc meridian being the thickest (294.0 ± 13.9 ㎛). The average GCC was thickest in the optic disc (105.3 ± 27.1 ㎛) and thinnest in the fovea meridian (42.8 ± 15.3 ㎛). The average RPE-INL of the fovea meridian (165.5 ± 18.3 ㎛) was significantly thicker than that of the other meridians (p < 0.05). The average RPE-ONL of the fovea, optic disc, yellow field, and red field were 91.2 ± 5.2 ㎛, 87.7 ± 5.3 ㎛, 87.6 ± 6.5 ㎛, and 91.4 ± 3.9 ㎛, respectively. RPE-INL and RPE-ONL thickness of the red field meridian did not change significantly with measurement location (p > 0.05). Conclusions: Measured data could be used as normative references for diagnosing pigeon retinopathies and further research on avian fundus structure.