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Role of Intergenic and 3'-Proximal Noncoding Regions in Coat Protein Expression and Replication of Barley yellow dwarf virus PAV

  • Moon, Jae-Sun;Nancy K. McCoppin;Leslie L. Domier
    • The Plant Pathology Journal
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    • v.17 no.1
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    • pp.22-28
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    • 2001
  • Barley yellow dwarf virus PAV (BYDV-PAV) has a 5.7-kb positive-sense single-stranded RNA genome that contains six open reading frames (ORFs). BYDV-PAV produces three subgenomic RNAs (sgRNAs). The largest of which encodes the coat, 17-kDa, and readthrough proteins from two initiation codons. To investigate the role of intergenic and 3'-proximal noncoding regions (NCRs) in coat protein (CP) expression and BYDV-PAV replication, a full-length infectious cDNA of the RNA genome of an Illinois isolate of BYDV-PAV was constructed downstream of the Cauliflower mosaic virus-35S promoter. Linear DNA molecules of these cDNAs were infectious, expressed the 22-kDa CP, and produced both genomic RNA sgRNAs in ratios similar to those observed in protoplasts inoculated with viral RNA. The portion of 5'NCR of sgRNA1 between ORFs 2 and 3 was not required for, but enhanced translation of CP from ORF3. Mutants containing deletions in the NCR downstream of ORF5 failed to replicate in oat protoplasts. These results indicate that an intact 3$^1$NCR is required for BYDV-PAV replication.

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A fiber beam element model for elastic-plastic analysis of girders with shear lag effects

  • Yan, Wu-Tong;Han, Bing;Zhu, Li;Jiao, Yu-Ying;Xie, Hui-Bing
    • Steel and Composite Structures
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    • v.32 no.5
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    • pp.657-670
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    • 2019
  • This paper proposes a one-dimensional fiber beam element model taking account of materially non-linear behavior, benefiting the highly efficient elastic-plastic analysis of girders with shear-lag effects. Based on the displacement-based fiber beam-column element, two additional degrees of freedom (DOFs) are added into the proposed model to consider the shear-lag warping deformations of the slabs. The new finite element (FE) formulations of the tangent stiffness matrix and resisting force vector are deduced with the variational principle of the minimum potential energy. Then the proposed element is implemented in the OpenSees computational framework as a newly developed element, and the full Newton iteration method is adopted for an iterative solution. The typical materially non-linear behaviors, including the cracking and crushing of concrete, as well as the plasticity of the reinforcement and steel girder, are all considered in the model. The proposed model is applied to several test cases under elastic or plastic loading states and compared with the solutions of theoretical models, tests, and shell/solid refined FE models. The results of these comparisons indicate the accuracy and applicability of the proposed model for the analysis of both concrete box girders and steel-concrete composite girders, under either elastic or plastic states.

Legal Issues in the Introduction of Compelled Decryption According to Device Unlock Limits

  • Chohee Bae;Sojung Oh;Sohyun Joo;Jiyeon Joo;KyungLyul Lee
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.17 no.2
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    • pp.591-608
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    • 2023
  • With the emergence of advanced encryption technologies such as Quantum Cryptography and Full Disk Encryption, an era of strengthening information security has begun. Users respond positively to the advancement of privacy-enhancing technology, on the other hand, investigative agencies have difficulty unveiling the actual truth as they fail to decrypt devices. In particular, unlike past ciphers, encryption methods using biometric information such as fingerprints, iris, and faces have become common and have faced technical limitations in collecting digital evidence. Accordingly, normative solutions have emerged as a major issue. The United States enacted the CLOUD Act with the legal mechanism of 'Contempt of court' and in 2016, the United Kingdom substantiated the Compelled Decryption through the Investigatory Powers Act (IPA). However, it is difficult to enforce Compelled Decryption on individuals in Korea because Korean is highly sensitive to personal information. Therefore, in this paper, we sought a method of introducing a Compelled Decryption that does not contradict the people's legal sentiment through a perception survey of 95 people on the Compelled Decryption. We tried to compare and review the Budapest Convention with major overseas laws such as the United States and the United Kingdom, and to suggest a direction of legislation acceptable to the people in ways to minimize infringement of privacy. We hope that this study will be an effective legal response plan for law enforcement agencies that can normatively overcome the technical limitations of decoding.

Chaotic vibration characteristics of Vertical Axis Wind Turbine (VAWT) shaft system

  • C.B. Maheswaran;R. Gopal;V.K. Chandrasekar;S. Nadaraja Pillai
    • Wind and Structures
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    • v.36 no.3
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    • pp.215-220
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    • 2023
  • We study the progressive full-scale wind tunnel tests on a high solidity vertical axis wind turbine (VAWT) for various tip speeds and pitch angles to understand the VAWT shaft system's dynamics using 0-1 Test for chaos. We identify that while varying rotor speed (tip speed) of the turbine, the system's dynamics change from periodic to chaotic through quasiperiodic and strange non-chaotic (SNA) states. The present study is the first experimental evidence for the existence of these states in the VAWT shaft system to the best of our knowledge. Using the asymptotic growth value Kc in 0-1 test, when the turbine operates at the low tip speeds and high pitch angles for low incoming wind speeds, the system behaves periodic (Kc ≈ 0). However, when the incoming wind speed increases further the system's dynamics shift from periodic to chaotic vibrations through quasi-periodic and SNA. This phenomenon is due to the dynamic stalling of blades which induces chaotic vibration in the VAWT shaft system. Further, the singular continuous spectrum method validates the presence of SNA and differentiates the SNA from chaotic vibrations.

Electronic Structures and Magnetism of MgCCo3(001) (MgCCo3(001)표면의 전자구조와 자성)

  • Jin, Ying-Jiu;Lee, Jae-Il
    • Journal of the Korean Magnetics Society
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    • v.14 no.3
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    • pp.94-98
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    • 2004
  • The electronic structures and magnetism of MgCCo$_3$(001) surface terminated by the plane with the MgCo-Term (Mg, Co terminated) and the CCo-Term (C, Co terminated) were investigated using the all-electron full-potential linearized augmented Plane-wave method. For the MgCo-Term, the magnetic moment of Co atom of the surface is strongly enhanced to 1.00$\mu$$_{B}$, while the magnetic moment of Co atom of the subsurface is similar to that of the center layers. For the CCo-Term, the magnetic moments of Co atoms are enhanced to 0.75 and 0.80$\mu$$_{B}$ for the surface and subsurface layers, respectively. The magnetic moments of C and Mg atoms are coupled antiferromagnetically to that of the neighbour Co atoms. From the calculated density of states, we see that the enhancements of magnetic moments of Co atoms are closely related to localization of the Co-3d states.

A Study on the Substantial impact of US high rate tariff policy on the Korean companies -Based on analysis of Article 301 of the US Trade Law -

  • Nam, Seon Mo
    • International Journal of Advanced Culture Technology
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    • v.7 no.4
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    • pp.63-68
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    • 2019
  • Recently, the United States and China have declared a 25% retaliatory tariff for the partner country products of 50 billion dollar scale. "Trade war" is getting full swing. Such conflicts between economic powers may spread to Japan like the domino phenomenon following the EU (European Union) and become bigger in the global trade war. As a result, Korea has an economic system with a high degree of external dependence, and there is an expert's analysis that it will become the largest victim of the global trade war. If the WTO Dispute Settlement Authority approves this US 301 retaliation measure in the same way as the past case (US-EU hormone-treated beef imports), the United States will not import any Chinese imported products Chinese products) can be imposed. If the US launches a special 301 or super 301, which is stronger than the regular 301, then China is very likely to enforce US retaliation against it, and the trade war between the two countries could become a reality. This phenomenon is likely to have a negative impact on Korean companies. In particular, Korea, which is highly reliant on intermediate goods exports to China, is expected to suffer a great deal of damage. Therefore, Korea needs flexible response at home and abroad, it is necessary to enhance the autonomy of companies and protect export industries. Adjusting corporate tax rate as well as domestic industry height will be one way. The long-term (21 months) trade war between the United States and China has resulted in economic uncertainty. The resulting damage must be compensated. It is necessary to prepare the compensation through the economic council between countries. In the future, the punitive damage compensation system should be introduced.

AN EXPERIMENTAL STUDY ON THE ACCURACY OF INTEROCCLUSAL RECORDING MATERIALS (교합관계 기록 재료의 정확성에 관한 임상적 비교 연구)

  • Park, Hong Yul;Chang, Ik Tae;Kim, Kwang Nam
    • The Journal of Korean Academy of Prosthodontics
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    • v.28 no.2
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    • pp.91-108
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    • 1990
  • The purpose of this study was to compare the maganitude of the discrepancies of the mounting errors in according to the states of dentitions, and to the superoinferior, anteroposterior, and rightleft driecetions. GROUP I. : Fourteen patients 22 to 26 years of age with a full complement of teeth, were used in the study. The criteria fro patient selection were a complete dentition, sparse restorarive treatment, and adequate posterior and anterior occlusan stops. And they had no sign and sympton at TMG area. GROUPII. : Eigth patients 37 to 62 years of age with bilateral free ends. The criteria for patient selection were Kennedy classification class 1 cases, and adequate posterior and anterior stops. And the opposite dentitions were a full complement of teeth. Irreversible hydrocolloid impresiion of each arch was taken of each patient. These were immediatel poured in stone and mounted on a Denar Mark II. Articulator with the arbitrary slidematic face-bow. With hand articulation th e mandibular cast was mounted to the maxillary cast in centric occlusion. Five types of interocclusal records were taken of each patient : (1) aluwax (2) baseplate wax; (3) znic oxide-eugenol pasts; (4) polyether (Ramitec); (5) modeling compound. All measurement of the five selected recording materials were compared with those of the hand-articulated full arch models in centric occlusion or maximum interdigitation. The results were as follows; 1. There were statistical differences in amount of devitation in according to the materials and the states of dentition. The amount of deviation of compound was the largest. 2. There were statistical differences in amount of deviation in complete dentition at all directions. The amount of diviation of compound was the largest. And at the right-left direction the amount of znic oxide-eugenol paste was larger than that of baseplate wax. 3. There was a statistical difference in amount of diviations in partial edentulous dentition at the superoinferior direction. The amount of deviation of compound was larger than that of znic oxide-eugenol paste. 4. There was as statistical difference in amount of deviations in partial edentulous dentition at the right-left direction. The amount of deviation of baseplate wax was larger that tnat of polyether. 5. There was not a statistical difference in amount of diviation in partial edentulous dentition at the anteroposterior direction.

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A Study on Flooding·Sinking Simulation for Cause Analysis of No. 501 Oryong Sinking Accident (제501 오룡호 침몰사고 원인분석을 위한 침수·침몰 시뮬레이션 연구)

  • Lee, Jae-Seok;Jung, Hyun-Sub;Oh, Jai-Ho;Lee, Sang-Gab
    • Journal of Navigation and Port Research
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    • v.41 no.6
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    • pp.451-466
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    • 2017
  • Deep-sea fishing vessel No. 501 Oryong was fully flooded through its openings and sunk to the bottom of the sea due to the very rough sea weather on the way of evasion after a fishing operation in the Bearing Sea. As a result, many crew members died and/or were missing. In this study, a full-scale ship flooding sinking simulation was conducted, and the sinking process was analyzed for the precise and scientific investigation of the sinking accident using highly advanced Modeling & Simulation (M&S) system of Fluid-Structure Interaction (FSI) analysis technique. To objectively secure the weather and sea states during the sinking accident in the Bering Sea, time-based wind and wave simulation at the region of the sinking accident was carried out and analyzed, and the weather and sea states were realized by simulating the irregular strong wave and wind spectrums. Simulation scenarios were developed and full-scale ship and fluid (air & seawater) modeling was performed for the flooding sinking simulation, by investigating the hull form, structural arrangement & weight distribution, and exterior inflow openings and interior flooding paths through its drawings, and by estimating the main tank capacities and their loading status. It was confirmed that the flooding and sinking accident was slightly different from a general capsize and sinking accident according to the simple loss of stability.

Reduced Order Luenberger State Observer Design for the Jackknifing Phenomenon Prevention of Articulated Vehicles using GPS (위성항법시스템을 이용한 연결식 차량의 잭나이핑 현상 예방을 위한 축소차수 상태관측기 설계)

  • Lee, Byung-Seok;Heo, Moon-Beom
    • Journal of Institute of Control, Robotics and Systems
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    • v.18 no.7
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    • pp.688-698
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    • 2012
  • This paper deals with ROLSO (Reduced Order Luenberger State Observer) design to prevent jackknifing phenomenon of articulated vehicles consisting of the tractor and semi-trailer by using GPS. In addition, by applying the regulator system using ROLSO feedback system, simulation's result presents that articulated vehicle's states are stabilized than the human's PR time (Preception Response time) rapidly. This simulation verifies that the automatic control of articulated vehicle's can be applied for the accident prevention for the time that the driver is unable to manage with the sudden accident. For this simulation, by using the equation of planar motion, the modeling of the articulated vehicle was performed. This modeling was expressed in the state space model. And FOLSO (Full Order Luenberger State Observer), ROLSO were designed by using the state space model of an articulated vehicle's dynamics.

A Preliminary Study of Korean Dual-Frequency SBAS

  • Yun, Ho;Han, Deokhwa;Kee, Changdon
    • Journal of Positioning, Navigation, and Timing
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    • v.3 no.1
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    • pp.11-16
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    • 2014
  • A Satellite Based Augmentation System (SBAS) is a representative differential GNSS system, which is used for the navigation performance improvement of Global Navigation Satellite System (GNSS) users. SBAS has been developed focusing on the securement of user integrity so that it can be used for the navigation in aviation fields. Accordingly, the development of SBAS has been completed, and it has been actively used in the United States, Europe, and Japan. As the new satellite of Global Positioning System (GPS) recently started to broadcast new civil signals (L5 frequency), the methods for improving user navigation performance in SBAS using this signal have also been studied. In Korea, to keep pace with these circumstances, full-scale SBAS development is expected to start in 2014, and studies on dual-frequency SBAS using L1/L5 frequencies will also be performed. In this study, before the full-scale development of dual-frequency SBAS in Korea, a simulation was performed to predict the performance and analyze the expected effects.