• Journal of Plant Biotechnology
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• 제29권2호
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• pp.129-134
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• 2002

포장에서 자란 '청양'의 상부 잎 절편체 유래의 캘러스에서 200개체의 유식물체를 분화시켜 194개체를 포장에 정식한 후 2년 동안 특성을 조사하였다. 잎의 형태는 모본과 같은 타원형이 66.1%였고, 모본과 다른 난형 22.2%, 도란형 7.2%, 장타원형 2.6%그리고 피침형이 2.1%였다. 또한 재분화된 구기자의 포장생육특성을 검정한 후 열매의 크기가 커지고 수량이 증대된 계통군 5계통 (99741, 99781, 99854, 99870, 99886)과 열매가 커진 계통군 4계통 (99774, 99831, 99840, 99849), 잎이 두꺼운 특징을 보인 2계통 (99747, 99783), 그리고 액아에서 신초가 많이 발생되는 다분지성의 초형변이 계통군 2계통 (99797, 99892) 등 13계통을 선발하였다. 열매의 변이를 나타낸 체세포 변이체간의 생산성을 비교한 결과, 대과와 다수확 계통으로 선발한 5계통들은 '청양'에 비해 과장이 2.1∼2.7 mm 길었으며, 10a당 생산량은 15∼30%가 증수되었다. 초형변이를 나타낸 개체간의 엽수량을 비교한 결과 대소계통인 CL1-48에 비해 99892계통이 7.2% 증수되었고 가시가 발생되지 않았으며 잎이 유연한 특징을 보였다.

• The Korean Journal of Ecology
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• 제26권4호
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• pp.173-180
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• 2003

To clarify whether the closely related species living in the same area is a population or populations ecologically, leaf morphology, specific leaf area, and fruit and seed production were studied in the natural group of sect. Pes-gallinaceua of Corydalis of Namhansansung area from 1999 to 2000. There were 352 plants in one square meter and total eight species or varieties were identified. Of the 352 plants, the number of C. turtschaninovii was the most with 103(29.3%), and that of C. ambigua was the next with 78(22.2%), and that of C. turtschaninovii var. fumariaefolia was the smallest with 9(2.6%). In the 28 plants having spotted leaves, central leaflet did not parted or again parted. The extent of partition with the plant was various from non-parted type to perfectly two-parted type (three leaflet). Between two extreme types, there were diverse types so that this character formed a gradient. The rate of length/breadth was in the range of 0.79~2.17. This character was related to the extent of leaflet partition but did not well expressed the distinguishing trait along a species. The number and the type of serration were diverse and there was no sharp borderline among the species or varieties. Ecological properties, specific leaf area, the number of fruit per plant, and the number of seed per fruit, varied with a wide range in a species or variety but differences between species or variety were not significant. Therefore, the Corydalis group studied was regarded as a population on the three criteria: (1) possibility of interbreeding, (2) continuity of leaf morphology, (3) irrelevance between character and species, (4) similarity of several ecological properties.

• The Plant Pathology Journal
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• 제39권3호
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• pp.235-244
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• 2023

Acidovorax citrulli (Ac) is a phytopathogenic bacterium that causes bacterial fruit blotch (BFB) in cucurbit crops, including watermelon. However, there are no effective methods to control this disease. YggS family pyridoxal phosphate-dependent enzyme acts as a coenzyme in all transamination reactions, but its function in Ac is poorly understood. Therefore, this study uses proteomic and phenotypic analyses to characterize the functions. The Ac strain lacking the YggS family pyridoxal phosphate-dependent enzyme, AcΔyppAc(EV), virulence was wholly eradicated in geminated seed inoculation and leaf infiltration. AcΔyppAc(EV) propagation was inhibited when exposed to L-homoserine but not pyridoxine. Wild-type and mutant growth were comparable in the liquid media but not in the solid media in the minimal condition. The comparative proteomic analysis revealed that YppAc is primarily involved in cell motility and wall/membrane/envelop biogenesis. In addition, AcΔyppAc(EV) reduced biofilm formation and twitching halo production, indicating that YppAc is involved in various cellular mechanisms and possesses pleiotropic effects. Therefore, this identified protein is a potential target for developing an efficient anti-virulence reagent to control BFB.

• 한국약용작물학회지
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• 제28권6호
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• pp.435-444
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• 2020

Background: As the Positive List System (PLS) is implemented in broad application to agricultural products, attention to the correct use of pesticides is also needed in the production of mulberry leaves and fruit. In this regard, three types of pesticides against mulberry popcorn disease were applied 2 - 3 times both in the field and greenhouses to prepare safety standards. Residual pesticide analysis was conducted on mulberry fruits and leaves. Methods and results: Three pesticieds, thiophanate-methyl, thiophanate-methyl·triflumizole and fluopyram registered as PLS pesticides for mulberry popcorn disease, were sprayed in the Wanju and in Buan regions, after which residual pesticide analysis was conducted using high-performance liquid chromatography (HPLC). As three pesticieds were either undetected or below the permissible level in mulberry fruit, demonstrating that they were suitable for safe spraying. However, 5.6 mg/kg of thiophanate-methyl was detected in the greenhouse after three application, which was slightly above maximum residue limit (MRL). Furthermore the level of thiophanate-methyl·triflumizole was higher than 0.2 mg/kg ("Gwasang No. 2" variety, spraying twice) or similar to 0.09 mg/kg ("Daesim" variety, spraying thrice) the permissible level (0.1T mg/kg) as the thiophanate-methyl was detected in mulberry leaves in the greenhouse. Conclusions: The spraying frequency for controlling mulberry popcorn disease in greenhouses should be limited to two times or less, especially when mulberry leaves are treated with thiophanate-methyl·triflumizole careful consideration is required if the leaves are to be used as food materials.

• 생물환경조절학회지
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• 제3권1호
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• pp.20-27
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• 1994

본 실험은 시설오이의 상품성 향상 및 생산성 증대를 위하여 주지형인 만능청장마디오이와 측지형인 사엽오이를 유인방법을 달리하여 재배하였으며 그 결과는 다음과 같다. 1. 유인방법 개선을 위한 오이의 저절위 착과특성을 조사하였던 바, 만능청장마디오이의 경우는 3마디까지, 사엽오이의 경우는 4마디까지가 수량 및 상품성이 불량하여 적과재배하는 것이 효과적이었다. 2. 오이의 유인방법 별 초기생육 및 과실의 개화시 자방중, 곡과도, 생장해석면에서 만능청장마디 오이는 횡유인, 종유인, 사면유인 순으로 좋았고 사엽오이는 횡유인, 사면유인, 종유인 순으로 좋게 나타났다. 3. 오이의 유인방법에 따라 초고별 상대조도와 적산엽면적지수로 본 작물의 수광태세는 만능청장 마디오이에서는 종유인과 횡유인이 사면유인보다 양호하였고 사엽오이는 횡유인, 종유인, 사면유인 순으로 나타났다. 4. 수량 및 품질은 만능청장마디오이와 사엽오이 모두에게 횡유인이 가장 우수하게 나타났다.

• 한국토양비료학회지
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• 제49권4호
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• pp.355-367
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• 2016

Soil salinization refers to the buildup of salts in soil to a level toxic to plants. The major factors that contribute to soil salinity are the quality, the amount and the type of irrigation water used. The presented review discusses the different sources and causes of soil salinity. The effect of soil salinity on biological processes of plants is also discussed in detail. This is followed by a debate on the influence of salt on the nutrient uptake and growth of plants. Salinity decreases the soil osmotic potential and hinders water uptake by the plants. Soil salinity affects the plants K uptake, which plays a critical role in plant metabolism due to the high concentration of soluble sodium ($Na^+$) ions. Visual symptoms that appear in the plants as a result of salinity include stunted plant growth, marginal leaf necrosis and fruit distortions. Different strategies to ameliorate salt stress globally include breeding of salt tolerant cultivars, irrigation to leach excessive salt to improve soil physical and chemical properties. As part of an ecofriendly means to alleviate salt stress and an increasing considerable attention on this area, the review then focuses on the different plant growth promoting bacteria (PGPB) mediated mechanisms with a special emphasis on ACC deaminase producing bacteria. The various strategies adopted by PGPB to alleviate various stresses in plants include the production of different osmolytes, stress related phytohormones and production of molecules related to stress signaling such as bacterial 1-aminocyclopropane-1-carboxylate (ACC) derivatives. The use of PGPB with ACC deaminase producing trait could be effective in promoting plant growth in agricultural areas affected by different stresses including salt stress. Finally, the review ends with a discussion on the various PGPB activities and the potentiality of facultative halophilic/halotolerant PGPB in alleviating salt stress.