• Journal of Plant Biotechnology
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• v.44 no.4
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• pp.379-387
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• 2017

Various sizes (0.2 ~ 1.2 mm) and developmental stages (referred to as Stage 1 ~ 3) of apical and lateral meristems were excised, together or separately, directly from dormant buds of apple 'Hongro'. They were mixed infected by Apple scar skin viroid (ASSVd), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV), which are major viruses attacking apples. A total of 31 callus lines (> 10 mm in diameter) were obtained by culturing the explants on Murashige and Skoog (MS) medium supplemented with 3% sucrose, 3.0 mg/L benzyladenine (BA) and 0.1 mg/L indole-3-butyric acid (IBA), and they were subjected to RT-PCR analysis for virus detection. A high rate of virus elimination (expressed as the percentage of calli that did not amplify during RT-PCR, i.e., RT-PCR negative calli per total number of calli obtained) was achieved for ACLSV (100%), ASSVd (93.7%), and ASPV (93.7%), whereas it was only 25.8% for ASGV. ASPV was detected in the presence of 2 ~ 3 bracts. Simultaneous virus elimination of ASSVd, ASPV, ACLSV, and ASGV occurred during the meristem culture, in which the early stages of the dormant buds (Stage 1) were used, because ASGV was mostly eliminated during that stage. The results of the present study will be valuable for the production of virus-free apple trees.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1512-1512
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• 2001

The University of Cordoba conducts since 1991 a breeding program to obtain new olive cultivars from intraspecific crosses. The objective is to obtain new early bearing and high-quality cultivars. In plant breeding, many seedlings must be tested to increased the chance of getting desirable genotypes. Therefore, fast, cheap and accurate methods of analysis are necessary. The conventional laboratory techniques are costly and time-consuming. Near Infrared Spectroscopy (NIRS) can satisfy the characteristics requested by plant breeders and offers many advantages such as the simultaneous analysis of many traits and cheap cost. The objective of this work was to asses the performance of NIRS to estimate oil fruit components (fruit weight, flesh moisture, flesh/stone ratio and oil flesh content in dry weight basis) and fatty acid composition in olive fruit. Genotypes from reciprocal crosses between ‘Arbequina’, ‘Frantoio’ and ‘Picual’ cultivars have been used in this study. A total of 287 samples, each from a single plant, were scanned using a DA-7000 Diode Array VIS/NIR Analysis System (Perten Instruments), which covers the visible and NIR range from 400-1700 nm. All samples were analysed for fatty acid composition (gas chromatography) and 220 for oil fruit components (oil content by nuclear magnetic resonance), 70% and 30% of samples were randomly assign for the calibration and validation sets respectively. The preliminary results shows that calibration for palmitic, oleic and linoleic acids were highly accurate with calibration and validation values of $r^2$ from 0.85 to 0.95 and 0.76 to 0.91 respectively. Calibration for palmitoleic and estearic acids were less accurate, probably because of the narrow range of variability available for these fatty acids. For the oil fruit components, calibration were high accurate for flesh moisture and oil flesh content in dry weight basis ($r^2$ higher than 0.90 in both calibration and validation sets) and less accurate for the other characteristics evaluated. The first results obtained indicate that NIRS analysis could be an ideal technique to reduce the cost, time and chemical wasted necessary to evaluate a large number of genotypes and it is accurate enough to use for pre-selecting genotypes in a breeding program.

• Journal of Plant Biotechnology
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• v.45 no.2
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• pp.90-101
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• 2018

Strawberry (Fragaria ${\times}$ ananassa) is a globally-cultivated and popular fruit crop, prized for its flavor and nutritional value. Sweetness, a key determinant of fruit quality, depends on the sugar composition and concentration. We selected eight strawberry cultivars based on the fruit soluble solids content to represent high and low sugar content groups. The average soluble solid content was $13.6^{\circ}Brix$ (Okmae, Geumsil, Aram, and Maehyang) and $2.9^{\circ}Brix$ (Missionary, Camino Real, Portola, and Gilgyung53), for the high and low sugar content groups, respectively. Sucrose was the main sugar in the cultivars with high sugar content, whereas fructose was the main component in the low sugar content cultivars. Fruit starch concentration ranged from $3.247{\pm}0.056$ to $3.850{\pm}0.055g/100g$, with a 12% higher concentration in the high sugar content cultivars. Additionally, we identified 41 sugar metabolism-related genes in Fragaria ${\times}$ ananassa and analyzed the relationship between their transcripts and the sugar accumulation in fruit. FaGPT1, FaTMT1, FaHXK1, FaPHS1, FaINVA-3, and FacxINV2-1 were highly expressed in the high sugar content cultivars, while FapGlcT, FaTMT2-1, FaPHS2-1, FaSUSY1-1, and FaSUSY1-2 were highly expressed in the low sugar content cultivars. In general, a greater number of genes encoding sugar transporters or involved in sugar synthesis were highly expressed in the high sugar content cultivars. Contrarily, genes involved in sugar degradation were preferentially transcribed in the low sugar content cultivars. Although gene expression was not perfectly proportional to sugar content or concentration, our analysis of the genes involved in sugar metabolism and accumulation in strawberries provides a framework for further studies and for the subsequent engineering of sugar metabolism to enhance fruit quality.

• Journal of Biosystems Engineering
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• v.37 no.3
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• pp.177-183
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• 2012

Purpose: In near infrared spectroscopy, interactance configuration of a light source and a spectrometer probe can provide more information regarding fruit internal attributes, compared to reflectance and transmittance configuration. However, there is no through study on the parameters of interactance measurement setup. The objective of this study was to investigate the effect of the parameters on the estimation of soluble solids content (SSC) and firmness of muskmelons. Methods: Melon samples were taken from greenhouses at three different harvesting seasons. The prediction models were developed at three distances of 2, 5, and 8 cm between the light source and the spectrometer probe, three measurement points of 2, 3, and 6 evenly distributed on each sample, and different number of fruit samples for calibration models. The performance of the models was compared. Results: In the test at the three distances, the best results were found at a 5 cm distance. The coefficient of determination ($R_{cv}{^2}$) values of the cross-validation were 0.717 (standard error of prediction, SEP=$1.16^{\circ}Brix$) and 0.504 (SEP=4.31 N) for the estimation of SSC and firmness, respectively. The minimum measurement point required to fully represent the spectral characteristics of each fruit sample was 3. The highest $R_{cv}{^2}$ values were 0.736 (SEP=$0.87^{\circ}Brix$) and 0.644 (SEP=4.16 N) for the estimation of SSC and firmness, respectively. The performance of the models began to be saturated when 60 fruit samples were used for developing calibration models. The highest $R_{cv}{^2}$ of 0.713 (SEP=$0.88^{\circ}Brix$) and 0.750 (SEP=3.30 N) for the estimation of SSC and firmness, respectively, were achieved. Conclusions: The performance of the prediction models was quite different according to the condition of interactance measurement setup. In designing a fruit grading machine with interactance configuration, the parameters for interactance measurement setup should be chosen carefully.

• Journal of Bio-Environment Control
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• v.10 no.4
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• pp.237-243
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• 2001

The experiment was conducted to investigate the effect of different mulching materials on the growth and quality of oriental melon in protected cultivation. Soil temperature, plant growth and fruit quality were affected by different mulching materials, clear polyethylene (P.E.) film (control), clear inset between black P.E. film, green P.E. film. and recycled ethylene vinyl acetate (E.V.A.) film. The highest night soil temperature was at 20 cm depth under green P.E. film, but was at 5 cm depth under other materials. Difference of soil temperature as affected by mulching materials decreased with increasing soil depth. Plant height, number of nodes, leaf area index (LAI), crop growth rate (CGR), and relative growth rate (RGR) at 30 days after transplanting were significantly greater in the recycled E.V.A. film treatment than in the other treatments. Fresh weight of weeds growth under the mulching materials was not affected by mulching material at 30 days after transplanting. However, it was the greatest under clear P.E. film mulching at 90 days after transplanting. Harvesting time of recycled E.V.A. film was 2 days earlier than that of clear P.E. film. Difference in fruit weight and length, and soluble solid content were not affected by the mulching materials. Marketable yield was 2,426 kg.10a$^{-1}$ in recycled E.V.A. film treatment, which was 6% greater than in clear P.E. film treatment.

• Korean journal of applied entomology
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• v.25 no.2 s.67
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• pp.63-70
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• 1986

Severity of incidence of white rot on apple fruit ranged from 5 to 16% and averaged 9% over major apple growing area in 1981. An isolate of Botryosphaeria ribis obtained from rotted apples developed lesions on leaves, branches and fruits of apple, pear, peach and grape in a series of wound inoculation test. B. ribis grew well on both potato sucrose agar and oatmeal agar. The best condition for vegetative growth on these two media was at $25{\sim}30^{\circ}C$ pH 4 and $10{\sim}15%$ sucrose content under light illumination. Rot development on fruit was first observed in the orchard at early August when sugar content in fruit reached 9.0%. Thereafter, number of rotted apples increased as sugar content increased. There was no correlation between the pH of juice of fruit and rot incidence. Infection on fruit began to occur as early as mid-June when young fruits were formed and infections were continued until harvest. When apple fruits were collected at 10-day intervals from the orchard beginning from early June and were wound-inoculated with B. ribis, rot lesion developed regardless of the stage of fruit growth. Incidence of white rot in the orchard was severe on Golden-delicious and Yukou, intermediate on Aoli, Fugi and Indo, and least on Jonathan and Red-delicious.

• Journal of Bio-Environment Control
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• v.12 no.1
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• pp.17-21
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• 2003

This experiment was conducted to find out the method of preventing decrease in the marketable yield of oriental melon (Cucumis melo L. var. makuwa MAKINO) under low light intensity. By maintaining low light of 400 $\mu$mol$.$m$^{[-10]}$ 2$.$S$^{-1}$ from 10 days after fruit set to fruit enlargement period, the photosynthetic rate and chlorophyll contents of leaf were reduced. Leaves which had no urea application showed largely decreased photosynthetic rate The content of soluble solids was lower$.$ in the low light than natural light treatment. Regardless of foliar application of urea, % fermentation fruits was under 4% in the natural light treatment and over 10% in the low light treatment. The less the fruit thinning, the greater was % fermentation fruits under low light condition. The % fermentation fruits were 39% and 40% in no fruit thinning treatment. The harvest was delayed under low light condition regardless of foliar fertilization. As the number of thinned fruits was decreased, the harvest time was delayed more. Marketable yield per plant sharply decreased under low light intensity. Compared with natural light, the yield under low light treatment was 16∼34%. The treatment fertilized with 0.5% urea on leaf had 34% greater harvest index of marketable yield than other treatments. In conclusion, when the long low light condition from 10th day after fruiting was forecasted, thinning two fruits out of six fruits and two times foliar fertilization with 0.5% urea should be applied.

• The Korean Journal of Mycology
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• v.34 no.2
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• pp.79-83
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• 2006

This study was made to investigate the difference of the shiitake productivity according to the use of sawdust, plug-shaped and liquid spawn. Contamination of medium was confirmed by eye. There was no contamination in the liquid spawn treatment. And, contamination rates in the sawdust spawn and plug-shaped spawn were 6.7%, respectively. Productivity of fruit-bodies was 725.1 g from sawdust spawn, 726.2 g from plug-shaped spawn and 573.9 g from liquid spawn until 3rd flushing of fruit-bodies. The number of fruit-bodies was 57.4 per medium of sawdust spawn, 64.3 plug-shaped spawn and 45.1 liquid spawn. At yield per medium, the percentage of media producing over 1,000 g was 13% in plug-shaped spawn and 7% in liquid spawn. However, there was no medium producing over 1,000 g of fruit-bodies in sawdust spawn. Fruit-bodies of $600{\sim}900g$ were yielded from 79% of sawdust spawn treatment, 53% plug-shaped spawn and 33% liquid spawn treatment. When compared the productivity of fruit-bodies, liquid spawn treatment appeared lower than sawdust spawn and plug-shaped spawn treatment at 1st flushing, but appeared higher than the others at 2nd and 3rd flushing.

• Korean Journal of Breeding Science
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• v.50 no.4
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• pp.497-503
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• 2018

A strawberry variety 'Arihyang' was derived as an artificial cross between 'Tochiotome' and 'Seolhyang' in 2014. The seedling and line selections were conducted from 2014 to 2015. Preliminary and advanced yield trials of '14-5-5,' which was the final selected line, were conducted from 2015 to 2017. 'Arihyang' is suitable for forced cultivation and has strong plant vigor, uniformly large-sized fruit, and a high yield compared to those of the check variety, 'Seolhyang' and 'Maehyang.' Especially, vitamin C was at a significant level, which was approximately 15% higher than that of 'Seolhyang.' The average number of flowers per first flower cluster was 10.5, which could reduce the labor of thinning fruit. Its fruit has a conical shape, dark red color, and glossy skin. The fruit was of good quality but has recommendations for harvest at the fully ripened stage. 'Arihyang' has intermediate resistant to phytophthora crown rot, but is susceptible to powdery mildew, gray mold, anthracnose, and fusarium wilt. It is reguired to manage major diseases and pests using optimum cultivation techniques and chemical control.

• Korean Journal of Agricultural and Forest Meteorology
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• v.23 no.4
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• pp.349-355
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• 2021

The effect of night temperature was investigated on mango (Mangifera indica cv. Irwin) for setting appropriate night temperature and managing mango cultivation in a greenhouse. Different night temperatures (10, 15, and 20℃) were treated from budding to fruit development in mango trees. As night temperature increased, the initiation of flowering, full blooming, fruiting, and fruit development tended to be accelerated. There were no significant differences in the growth of shoots flushed after the fruit harvest among trees treated with different night temperatures. The lengths of the flower stalk were the shortest at 10℃ of night temperature; however, there were no significant differences between 15℃ and 20℃ of the night temperature. The length, diameter, and weight of mango fruits were higher at 15℃ and 20℃ than 10℃. Soluble solid contents and acidities showed no difference; the firmness and skin color were better as the night temperature increased. Accordingly, setting the minimum night temperatures to 15℃ or 20℃ can increase the number of fruits and produce high-quality fruits when cultivating mango in the greenhouse. Even the minimum night temperature to 15℃ can reduce heating costs in the greenhouse.