• 생약학회지
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• 제36권2호통권141호
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• pp.102-108
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• 2005

We have studied the physico-chemical change and pharmacological transformation of traditional herbal medicines with processing. Evodia fruit (EF) has processed for the purpose of detoxification and reduction of its bitter taste. There are various methods of processing EF in Oriental medicinal reference. Among them, we processed EF according to the method of Sang-han theory, the most famous medicinal reference. We processed EF with washing in hot water, and then dried. Processed EFs(PEFs) were prepared according to the above procedure through repetition of 1,3,5 and 7 times respectively. The contents of weight loss, water extract, diluted ethanol extract, ether extract, total ash, acid insoluble ash, alkaloids and limonin in non-processed EF (NPEF) and PEFs were examined. The weight loss, and contents of water extract and dilute ethanol extract in PEF showed decrease in proportion to increase of washing time, while the contents of ether extract, total ash and acid insoluble ash showed little change as compared with those of NPEF. And the content of evodiamine and rutaecarpine was not changed distinctly. However, the content of limonin decreased in the final processed material in proportion to increase of washing times with hot water. And the intense of bitter taste in PEF was also reduced. These results were ascribed to the flowing-out of the water-soluble portion. The biological activities of NPEF and PEF were also investigated. In the test of DPPH scavenging effect, xanthine oxidase inhibition effect and TBA-Rs effect, PEF was more effective than NPEF in vitro. Also, both NPEF and PEF showed potent analgesic and anti-inflammatory effects against in mice. Especially, PEF by 3 times washing with hot water was more effective than other PEFs. From these results, it is considered that PEF should be prepared by 3 times washing with hot water.

• 한국작물학회지
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• 제60권3호
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• pp.366-373
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• 2015

This study was conducted to clarify the antimicrobial effect, antioxidant and tyrosinase inhibitory activities of the biological composition having the Phytolacca americana, and to enhance the natural materials utilization of foods and cosmetics. The antimicrobial activities of the different parts of P. americana were evaluated using the agar diffusion test. The antimicrobial activity of P. americana was relatively high in Malassezia furfur known as a skin fungi and Vibrio parahaemolyticus compared to Escherichia coli and Staphy-lococcus epidermidis. However, the antimicrobial activity in Vibrio parahaemolyticus did not show at all parts of P. americana. Both the DPPH radical scavenging activity and ABTS radical scavenging activity have been increased with the higher concentration of methanol extract. In particular, leaf extract of P. americana exhibited the highest activity both ABTS radical scavenging activity and DPPH radical scavenging activity. The nitrite scavenging activity was decreased when the pH was changed from pH 1.2 to pH 6.0. The highest nitrite scavenging activity was exhibited from the methanol extract of fruit, followed by root, stem, and leaf at pH 1.2. However, the nitrite scavenging activity at pH of 6.0 was not almost detected. All plant parts of P. americana showed tyrosinase inhibitory activity. The highest activity was found in the stem, and followed by root, leaf, and fruit in order. These tyrosinase inhibitory activity was progressively increased in a concentration-dependent manner. In this experiment on the methanol extracts of different organ from P. americana, we confirmed that the extract of P. americana showed potent tyrosinase inhibitory activity. Taken together, we conjectured that the P. americana had the potent biological activities, therefore this plant having various functional components could be a good material for development into source of natural food additives and cosmetics.

• 한국식품영양과학회지
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• 제46권1호
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• pp.140-145
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• 2017

본 연구에서는 식품으로 활용하기 위해 제조된 잣송이 추출물의 이화학적 품질 특성과 항산화 활성을 탐색하였다. 잣송이 추출물은 수분과 탄수화물이 구성성분 대부분을 차지하고 있었으며, 특히 대조군으로 사용된 매실 추출물보다 수분함량은 낮고 당 함량은 높아 유의적으로 높은 점도를 나타내었다. 또한, 갈색도와 색도는 모두 낮아 매실 추출물과 비교해 숙성 중 Maillard 갈변 반응의 정도가 상대적으로 낮았음을 시사해주었다. 잣송이 추출물은 매실 추출물보다 플라보노이드 함량은 유의적으로 다르지 않았으나 유기산 함량과 갈변도는 1/10 수준으로 유의적으로 낮았고, 총환원력은 매실 추출물의 60% 수준으로 측정되었다. 그런데도 잣송이 추출물에서 매실 추출물과 유사한 높은 금속 소거능과 라디칼 소거 활성이 관찰되었다. 또한, 잣송이 추출물 속 플라보노이드 총량보다도 많은 양인 6.6 mM quercetin과 비교할 때 2배나 높은 SOD 유사 활성을 나타내었다. 이는 잣송이 추출물에 quercetin보다 항산화 활성이 높은 플라보노이드가 존재할 가능성을 보여주며, 플라보노이드 조성에 대한 후속연구의 필요성을 시사해주었다. 본 연구는 잣송이 추출물이 제조법과 용도가 유사한 매실 추출물에 버금가는 항산화 활성을 부여하면서도 상대적으로 높은 당 함량과 낮은 유기산 함량으로 매실 추출물과는 관능적 측면에서 차별화될 수 있는 식품으로서의 활용 가능성을 보여주었다.

• 한국식품영양학회지
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• 제22권4호
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• pp.485-491
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• 2009

In order to examine the nutritional value of prickly pear(Opuntia humifusa), contents of ash, protein, fat, minerals and vitamins were determined on freeze-dried stem, fruit, seed and root from plants harvested in autumn. The average moisture contents for stem, fruit, seed and root were 67~87%. Crude ash content determined on dry weight basis was 2~3%. Crude protein existed mostly in seed(2.95%) and root(2.37%). Crude fat was detected mainly in seed(4.49%). Contents of major minerals(mg/100 mg dry weight) was generally higher in stem. Ca in stem(4,142.30) and fruit(2,790.86) were much higher than in seed(43.37). P in stem, seed and fruit were 448.19, 263.20 and 161.59, respectively. Stem also displayed more abundant Mg(1,110.86), Zn(35.62) and Mn(37.07). However, fruit contained higher amounts of Fe(13.38) and Se(0.15). Vitamin A was negligible in all plant parts. Vitamin E contents in fruit and stem were 1.78 mg and 1.22 mg/mg dry weight, respectively. Vitamin C was detected mostly in fruit(445.40) and stem(260.94). Use of a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-based microtiter assay of cell viability demonstrated an anti-proliferative effect of O. humifusa extract on the MCF-7 estrogen-dependent human breast cancer cell line.

• Preventive Nutrition and Food Science
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• 제15권1호
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• pp.57-66
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• 2010

Passion fruit fiber pectin gels represent a new alternative pectin source with potential for food and non-food applications on a commercial scale. Pectic polysaccharides were extracted from passion fruit (Passiflora edulis) fiber using citric acid as a clean catalyst and autoclaved for 20 to 60 min at $121^{\circ}C$. The best condition of pectin yield with the highest molecular weight was obtained with 1.0% of citric acid (250 mg/g dry passion fruit fiber pectin) for 20 min of autoclaving. Spectroscopic analyses by Fourier transform infrared, enzymatic degradation reactions, and ion-exchange chromatography assays showed that passion fruit pectin extracted for 20 min was homogeneous high methoxylated pectin (70%). Gel permeation analysis confirmed that the pectin extract obtained by autoclaving by 20 min showed higher molecular weights than those autoclaved for 40 and 60 min. Passion fruit pectin extracted for 20 min was enzymatically modified with fungal pectinmethylesterase to create restructured gels. Short autoclave treatment (20 min) with citric acid as extractant resulted in a significant increase of gel strength, improving pectin extraction in terms of functionality. The treatment of solubilized material (pectic polysaccharides) in the presence of insoluble material (cellulose and hemicellulose) with pectinmethylesterase and calcium led to the creation of a stiffer passion fruit fiber pectin gel, while syneresis was not observed.

• 한국식품과학회지
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• 제29권5호
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• pp.847-853
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• 1997

손바닥 선인장(Opuntia ficus-indica var. saboten)의 성분특성을 조사하여 식품으로의 이용 가능성을 검토하고자 하였다. 선인장과 알로에의 주성분은 가용성 무질소물이었다. 총무기질 함량은 선인장 줄기, 열매와 씨가 각각 9400.8, 6151.2와 1096.8 mg%이며, 주요 무기질은 Ca, P, Mg이었다. 주요 유리 아미노산은 선인장 열매인 경우 tyrosine, proline과 arginine이, 줄기는 glycine과 arginine이 씨의 경우 glutamic acid이었다. 총 아미노산중 주요 아미노산은 열매의 경우는 glutamic acid가, 줄기는 glycine과 arginine이었으며, 씨는 glutmic acid와 arginine이었다. 비타민 C는 열매와 줄기가 각각 163.8과 71.2 mg% 존재하나 씨에는 존재하지 않았으며, 비타민 A는 모든시료에서 검출되지 않았다. 총 폴리페놀 화합물과 플라보노이드 함량은 추출방법에 따라 다르며, 총 폴리페놀 화합물의 경우 선인장 열매가 줄기와 씨보다 함량이 높았으며, 총 플라보노이드 함량은 줄기와 열매에서 비슷하였다.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2006년도 제47회 학술심포지움 및 추계국제학술대회
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• pp.85.1-85.1
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• 2006

• 한국자원식물학회지
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• 제28권2호
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• pp.145-152
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• 2015

본 연구에서는 까마귀쪽나무 잎과 열매 추출물의 항염증 활성을 비교하고자 마우스 대식세포주인 RAW 264.7 cell에서 LPS에 의해 유도된 COX-2/PGE2와 iNOS/NO 및 염증성 cytokine 인 TNF-${\alpha}$, IL-$1{\beta}$, IL-6의 생성 억제 정도를 확인하였다. 그 결과, 동일한 농도 조건($10{\mu}g/ml$)에서 까마귀쪽나무의 열매 추출물이 잎 추출물에 비해 NO, $PGE_2$, iNOS, 염증성 cytokine인 TNF-${\alpha}$, IL-$1{\beta}$, IL-6의 생성량 모두에서 억제 효능이 우수하게 나타났다. 따라서 까마귀쪽나무가 보여주는 항염증 효과적 측면과 안전성의 두가지 측면을 고려할 때, 까마귀쪽나무의 열매 추출물이 저농도에서 잎 추출물보다 항염증 효과가 우수하거나 동등하며, 그 안전성이 크다고 판단됨으로 까마귀쪽나무의 열매추출물이 항염증 효능을 가진 기능성 식품 소재로써 개발 가능성이 있음을 제시한다.

• 한국식품저장유통학회지
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• 제3권2호
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• pp.121-129
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• 1996

Plum highlighted as a health food is needed to diversify the processed products because labor storage is big problem since the fruit was producted massively in June. The Plum was extracted by the pressing type extractor after washing, drying and removing the seed by seed separator. The crude extract was concentrated with stainless steel vessel at different temperature and stirring speed. This study was obtained as follows. The sugar content of fresh plum concentrated extract was 55.3~58.3$^{\circ}$Brix, and of the freezing plum concentrated extract was 75.5~70.3$^{\circ}$Brix. In color difference, the freezing plum concentrated extract was more deep black than fresh plum. In change patten of pH, it was decreased as concentration was proceed. The final pH was 2.3~2.2 in fresh plum, and 1.8~2.2 in freezing plum. The total acid content of fresh plum concentrated extract and the freezing plum was 45.4~47.8, 60.3~60.9%, respectively. The content of evaporation at 85$\pm$5$^{\circ}C$ was constant irrespective of stirring speed. The yield of extraction of fresh plum was higher than freezing plum. According to this results, the use of stainless vessel, 50rpm, which gave a highly qualified plum concentrated extract.

• 한국식품영양과학회지
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• 제38권2호
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• pp.154-159
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• 2009

알코올에 의해 유도된 간 손상에 대한 지구자 추출물의 보호효과를 연구하였다. HepG2/2E1 세포에서 알코올로 유도된 ROS 생성과 산화적 손상에 대한 지구자 추출물 보호효과를 확인하였다. C57BL/6마우스를 대조군(NC), 알코올군(ET), 알코올과 지구자 추출물 1 g/kg body weight 투여군(ET-HD)으로 나누었다. 5 g/kg body weight의 알코올을 1주일간 ET와 ET-HD군에 투여하였다. 알코올 투여는 혈청 alanine amintransferase(ALT), aspartate aminotransferase(AST) 및 alkaline phosphatase(ALP)를 증가시키고, 지구자 추출물은 이러한 간 기능 지표효소의 증가를 억제시켰다. 간조직의 항산화 효소 활성은 알코올 투여에 의해 감소되었고, ET-HD군에서 SOD 및 GST 활성은 ET군과 비교하여 통계적으로 유의하게 높아졌다. GSH 함량은 ET군에서 NC군에 비하여 유의적으로 낮아졌고, ET-HD군에서 ET군과 비교하여 통계적으로 유의하게 높아졌으며, NC군과 유사한 함량을 나타내어 간 보호 효과를 확인할 수 있었다. 지질과산화물 함량은 ET-HD군과 NC군이 유사한 함량을 나타냄으로써 알코올에 의해 유도된 지질과산화물 증가에 의한 간손상으로부터 지구자 추출물의 보호 효과를 보여 주었다. 이상의 결과로부터, 지구자 추출물은 세포 및 동물 모델에서 알코올로 유도된 간 손상으로부터 항산화 방어 대사의 증가와 지질과산화율의 감소에 의해 간세포 보호 활성을 나타냄을 확인하였다. 이에 지구자 추출물은 알코올성 간 손상으로부터 보호 효과를 갖는 소재로 활용될 수 있을 것으로 사료된다.