Rubus coreanus Miquel (RCM), a type of red raspberry, grows wild in Korea and China and its unripe fruit is used as a folk medicine for the treatment of impotence and as a diuretic. RCM was extracted with methanol and then further fractionated it into for different types. In this study, we investigated the antioxidant activity of a RCM extract (ext.) and its fraction (fr.). DPPH free radical scavenging activity assay, total polyphenols contents, total flavonoids contents assay were used to analyze antioxidant activity. The DPPH free radical scavenging activity $(RC_{50}:1.67{\mu}g/ml)$ and total polyphenols contents $(546.25{\mu}g/mg)$ were higher in butanol fraction than in other fr. And total flavonoids contents was higher in ethylacetate fr. $(141.78{\mu}g/mg)$. We applied comet assay to measure the DNA damage in the individual cells and exposed time course at $IC_{50}$. Comet assay is a rapid and sensitive fluorescent microscopic method to examine DNA damage and repair at individual cell level. The butanol fro from RCM significantly induced 54.12%, 57.95% of DNA damage after treated RCM for 8 hr. In conclus
This study was performed to investigate the effect of fermented Hovenia dulcis Thunb fruit water extract on biomarkers for acute (a) ethanol-induced hangover and chronic (c) ethanol-induced liver injury in rats. For acute ethanol-induced hangover, the rats were administered distilled water (D.W., 10 mL/kg body wt.), Hovenia dulcis Thunb fruit water extract (HWE, 400 mg/10 mL/kg body wt.) and fermented HWE (FHWE, 400 mg/10 mL/kg body wt.), respectively, before 40% ethanol (5 g/kg body wt.) was administered. For chronic ethanol-induced liver injury, the rats were randomly divided into the normal control (cNC), ethanol (cET), cET-HWE and cET-FHWE groups. The cNC and cET groups were administered D.W. (10 mL/kg body wt.) before 40% alcohol (5 g/kg body wt.) was administrered for 21 days. The cET-HWE and cET-FHWE groups were administered HWE (400 mg/10 mL/kg body wt.) and FHWE (400 mg/10 mL/kg body wt.), respectively before 40% ethanol (5 g/kg body wt.) administration for 21 days. For acute ethanol-induced hangover, the serum alcohol and acetaldehyde concentrations were more significantly reduced in the aHWE and aFHWE groups than in the aET group. Moreover, the effect of FHWE was greater than that of HWE. For chronic ethanol-induced liver injury, the serum ethanol, acetaldehyde, ${\gamma}$-glutamyl transpeptidase (${\gamma}$-GTP) levels and the hepatic lipids concentration more significantly dropped in the cET-HWE and cET-FHWE groups than in the cET group. The FHWE administration showed faster recovery of the serum glucose concentration than in the cET and cET-HWE groups. The body weight reduction tended to normalize in the cET-HWE and cET-FHWE groups, which is ideal for chronic ethanol administration. These results suggest that FHWE has a protective effect against ethanol-induced liver damage, as evidenced by its ability to lower the serum ethanol and acetaldehyde concentrations after alcohol administration, and by its ability to decrease the level of ${\gamma}$-GTP and hepatic lipids. FHWE also elevated serum glucose concentration. Therefore, FHWE is effective in reducing ethanol-induced hangover and can play a beneficial role in the treatment of ethanol-induced liver damage as well as body weight reduction.
In Yesan-gun, Korea's main apple-producing region, the area of apple cultivation and yield are declining. In particular, the worsening quality of fruits due to unusually high temperatures amid recent climate change has also become a major challenge for apple orchards located on flatlands. The objective of this research is to investigate quality changes of apples according to different growing environments, depending on the shade of the sun, by covering the trees with different colors of wind nets. A white and blue wind nets with a hole size of 2 × 2 mm is installed on two experimental trees, 17-year-old 'Fuji' and 'Hongro', which are planted 1.5 m × 3.5 m in the north-south direction. Treatment of wind nets effectively lowered fruit surface temperature regardless of apple variety. When measuring the temperature of the fruit surface at 2 pm, the temperature of the air was 34.8℃, but the 'Fuji' of the untreated blocks was the highest at 40.0℃, while the blue wind net and the white wind net were significantly lower at 34.9℃ and 36.6℃, respectively. In 'Hongro', the results showed that the surface temperature was effectively lowered by recording 38.3℃ for the blue wind net and 38.5℃ for the white wind net treatment when the untreated one was 44.2℃. According to the color difference in 'Fuji', the skin redness (a⁎) was the lowest with untreated control at 16.5, but the blue and white wind net treatment higher at 18.0 and 19.3, respectively. In 'Hongro', the white wind net treated fruit also showed a much higher skin redness than the untreated control of 28.1, showing much higher a⁎ of 34.9. Sunburn damage in 'Fuji' apples amounted to 9.4% in untreated control. However, the blue and white wind net treatment revealed to 3.8% and 4.2%, respectively. In 'Hongro', those damage in the fruits treated with blue or white wind net, accounted for only 8.8% and 12.4%, respectively, significantly lower than 28.8% occurrence of untreated one. And, these results were understood to be the result of low UV radiation being blocked by the treatment of wind nets.
A wax-typed pheromone dispenser has been developed and applied to control outbreak of the oriental fruit moth, Grapholita molesta, in apple orchard. To optimize its application technique, this study analyzed effect of different amounts of the pheromone dispenser on mating disruption('MD') of G. molesta. Different pheromone dispenser amounts significantly influenced the MD effect assessed by cumulative male adult catches monitored respectively by sticky delta trap and food trap, and resulted in differential damage on host plants. In a field test during entire growing season, a standard amount(120 g per 0.117 ha) of wax-typed pheromone dispenser was proved to be effective to suppress outbreak of G. molesta adults and to prevent host plant damage as much as a current commercial MD product($Isomate^{(R)}$). This study also demonstrated an effectiveness of deployment of food trap barriers around MD-treated area to prevent immigration of mated females from outside untreated areas. These results indicate that the wax-typed pheromone dispenser can be applied to control field G. molesta populations and its co-application with fuod trap barriers would be optimal to maximize MD efficacy.
Kim, Min-Gi;An, Min-Sil;Park, Jong-Ho;Lee, Cho-Rong;Lee, Sang-Beom;Park, Kwang-Lai;Hong, Seung-Gil
Korean Journal of Organic Agriculture
/
v.25
no.3
/
pp.603-617
/
2017
The occurrence and management of disease and pests in six organic peach orchards were surveyed from March 2015 to March 2017. In this period, the number of certified organic and non-chemical peach farms increased to 65.5% and 31.7%, respectively. Certified organic peach farms were selected based on more than $4,000m^2$ of cultivation area and three tons of production, and their cultivation status was examined. All of the farms were either cultivated green manure crop or sod, and limited vegetation control to a minimum. For the management of soil nutrients, many farmers used livestock manure, oilcake and self-manufacturing liquid fertilizer. It was surveyed that bordeaux mixture, lime sulfur, pheromone for mating disruption of moths and plant extract were used for disease and pest control. The damage caused by the pests and diseases were 31.6% and 24.1%, respectively. The oriental fruit moth showed the highest damage rate (13.5%) in the organic peach orchards, followed by the brown rot (13.0%), peach fruit moth (7.3%) and bacterial shot hole (7.3%).
Biological control of thrips with a predatory bug species, Orius sauteri Poppius, was evaluated in an eggplant greenhouse, To study the biological control strategy of thrips. three Plots were established: Orius sauteri released plot without pesticide treatment (NRT), pesticide treated plot (PAT), and natural enemy removed plot (RNT) The nymphs of O. sauteri were found on the upper side of leaves in NRT at 21 days after the first release of O. sauteri. The density of thrips were dropped and maintained at lower level in NRT than in RNT. Average damage index of NRT, PAT and RNT was 1.35, 1.21 and 2.90 and the percentage of damaged fruit ratio of those was 70.3, 68.6 and 99.0%, respectively. The damage index and percentage of damaged fruit in NRT were slightly higher than those of PAT, but much lower than in RNT. Taylor's band Iwao's $\beta$ were greater than 1 for both populations. indicating aggregated distribution. Spatial coincidence index of O. sauteri was high most of the time even at low densities of both populations. The refuge index for thrips decreased as O. sauteri density increased. Results of this study suggest that O. sauteri is a promising natural enemy for thrips control on eggplant.
Korean Journal of Agricultural and Forest Meteorology
/
v.23
no.3
/
pp.149-155
/
2021
Early warning systems for weather risk management in the agricultural sector have been developed to predict potential wind damage to crops. These systems take into account the daily maximum wind speed to determine the critical wind speed that causes fruit drops and provide the weather risk information to farmers. In an effort to increase the accuracy of wind risk predictions, an artificial neural network for binary classification was implemented. In the present study, the daily wind speed and other weather data, which were measured at weather stations at sites of interest in Jeollabuk-do and Jeollanam-do as well as Gyeongsangbuk- do and part of Gyeongsangnam- do provinces in 2019, were used for training the neural network. These weather stations include 210 synoptic and automated weather stations operated by the Korean Meteorological Administration (KMA). The wind speed data collected at the same locations between January 1 and December 12, 2020 were used to validate the neural network model. The data collected from December 13, 2020 to February 18, 2021 were used to evaluate the wind risk prediction performance before and after the use of the artificial neural network. The critical wind speed of damage risk was determined to be 11 m/s, which is the wind speed reported to cause fruit drops and damages. Furthermore, the maximum wind speeds were expressed using Weibull distribution probability density function for warning of wind damage. It was found that the accuracy of wind damage risk prediction was improved from 65.36% to 93.62% after re-classification using the artificial neural network. Nevertheless, the error rate also increased from 13.46% to 37.64%, as well. It is likely that the machine learning approach used in the present study would benefit case studies where no prediction by risk warning systems becomes a relatively serious issue.
Sung, Gyoo Byung;Kim, Yong Soon;Kim, Kee Young;Ji, Sang Duk;Kim, Nam Sook
Journal of Sericultural and Entomological Science
/
v.53
no.1
/
pp.19-28
/
2015
Though mulberry fruit is known to a by-product that was produced from mulberry tree after harvesting leaves for silkworm rearing, as a yield and consumption of mulberry fruit was increased, it has been fixing to a new income crop. But, a stable production of mulberry was issued on damage by the weather disaster, so it is requires that agricultural disaster insurance import. So, in this study as a basic research for a importation of agricultural disaster insurance, the cultivation of mulberry for the production of mulberry fruits, the shape of tree, the number of a bearing fruit from each cultivar, the occurrence of mulberry fruit sclerotic disease, and a yield and the market price of mulberry fruits were investigated and that results obtained were as follows; The number of mulberry tree each 10a was abundant in Buan and Jeongeup region that cultivated a suitable cultivar on low cut shape, however, a large cultivated Gochang region was little. The number of a bearing fruit per 1m on the branch was highest in the variety of Cheongilppong at Gochang. Yield of mulberry fruits each 10a was Cheongilppong at Gochang, followed by Iksuppong at Sangju. Average sale price of the mulberry fruits was high in the order of Gwasang No. 2, Suwonppong, Iksuppong, and Cheongilppong.
Kim, JunHyeok;Park, YoSup;Kwon, YongHee;Jung, MyungHee;Park, Hee-Seung
Horticultural Science & Technology
/
v.35
no.2
/
pp.170-177
/
2017
This study was conducted to estimate the light pollution damage caused by night-break lighting in 'Kyoho' grapes. Night irradiation was performed every night for four hours (10 p.m. to 2 a.m.) from the full bloom to the harvest stage using red, blue, and white lamps as artificial light. Fruit yield, soluble solid content, anthocyanin content, and chlorophyll a content were affected by night irradiation. The soluble solid content of the control was $17.5^{\circ}Brix$, approximately $1^{\circ}Brix$ higher than the red ($16.4^{\circ}Brix$), blue ($16.2^{\circ}Brix$), and white light treated grapes ($16.3^{\circ}Brix$). The anthocyanin content of the skin was also higher in the control at $4.08{\mu}g{\cdot}cm^{-2}$ compared to the red ($3.14{\mu}g{\cdot}cm^{-2}$), blue ($2.47{\mu}g{\cdot}cm^{-2}$), and white ($2.82{\mu}g{\cdot}cm^{-2}$) light treated samples. On the other hand, the chlorophyll a content of the control was the lowest at $0.268{\mu}g{\cdot}cm^{-2}$ as compared with the red ($0.339{\mu}g{\cdot}cm^{-2}$), blue ($0.345{\mu}g{\cdot}cm^{-2}$), and white ($0.372{\mu}g{\cdot}cm^{-2}$) light treated samples. Considering that higher soluble solid contents, higher accumulation of anthocyanin, and lower chlorophyll a contents are factors involved in fruit maturation, night irradiation may delay fruit maturation and red light treatment may result in decreased yield. Our results confirmed that night-break lighting regardless of the wavelength provoked light pollution in 'Kyoho' grapes. Therefore, fruit maturation may be poor in the presence of artificial light, including streetlamps, in neighboring vineyards.
We prepared extracts from bark, leaf and fruit of Sorbus commixta Hedl using by water and ethanol. To test for mutagenicity and antimutagenicity on extracts, we used Rec assay and Ames test. The result of Rec assay, the extracts of the Sorbus commixta Hedl did not show a DNA-damage activity. The results of Ames test were provided that extracts of Sorbus commixta Hedl showed $43{\sim}73\;%$ antimutagenic activities. In the inhibition ratio of the growth of several human cancer cells (A549, HepG2, MCF7), 91% of MCF7 cell growth, 94% of A549 cell growth and 91% HepG2 cell growth were inhibited by adding 1mg/ml of fruit ethanol extracts, bark ethanol extracts and fruit ethanol extracts, respectively. There was a little cytotoxicity on human normal hephatocyte (WRL68), extracts(1mg/ml) showed over the 70% survival.
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