• Korean Journal of Agricultural Science
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• v.46 no.4
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• pp.897-905
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• 2019

To compare the composting characteristics of food waste supplemented with various bulking agents, aerated composting was performed by mixing sawdust, ginkgo leaves, insect feces, and mushroom waste at ratios of 6 : 4 (w/w). The initial temperatures (day after treatment [DAT] 3) of the sawdust, ginkgo leaves, insect feces and the mushroom waste mixtures were 39, 58, 65, and 51℃, respectively. The DAT 3 temperature was the highest in the food waste-insect feces mixture (65℃) and the lowest in the sawdust one (39℃). However, the insect feces treatment was terminated at DAT 21 because of a high water content (70.92%). The water content (DAT 56) of the composted food waste supplemented with sawdust, mushroom waste, and ginkgo leaves stood at 51.28, 39.81, and 44.92%, respectively. Therefore, the fully mature composts satisfied the water content requirement of less than 55% as recommended in the fertilizer standards of the RDA of Korea. The results of the CoMMe-101, Solvita and seed germination index methods indicate that the mushroom waste and ginkgo leaves treatments matured relatively quicker than that of the sawdust one. Based on the above observations, it is concluded that the mushroom waste and ginkgo leaves are more effective bulking agents compared to sawdust and as such, are recommended as suitable replacements for sawdust in food waste composting.

• Korean Journal of Agricultural Science
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• v.46 no.4
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• pp.931-939
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• 2019

Biochar is a solid carbon material made by pyrolyzing a biomass under limited oxygen conditions. Biochar has been reported to confer various benefits, such as increased soil productivity, pollutant absorption, and reduced greenhouse gas. In this study, oak pyrolyzed at 600℃ for 3 hours was either powdered or pelleted. Each of the biochar types was added to the soil at a rate of 2%. The control did not receive any biochar while a combination of the biochar and NPK treatment (biochar 2% + NPK) was also included. The cherry tomatoes were grown in greenhouse pots for 50 days to compare the growth characteristics of the different treatments. The cherry tomato with the powdered biochar 2% + NPK treatment had the heaviest plant fresh shoot weight of 276.4 g and the highest chlorophyll content of 59.3 SPAD. The control had the lightest plant fresh shoot weight of 44.2 g and a chlorophyll content of 26.5 SPAD. Both forms of biochar affected the chemical properties of the soil, increased the pH, electrical conductivity, available phosphate, total carbon and total nitrogen and positively influenced the cherry tomato growth and productivity. From the above results, therefore, both biochar forms are suited for use as soil amendments.

• Korean Journal of Agricultural Science
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• v.47 no.1
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• pp.11-18
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• 2020

Eggs are likely to be used in agriculture because they can provide enough nutrients for crop growth. Statistics show that a large number of eggs are lost due to breakage before reaching the final consumer. The purpose of this study was to make a natural liquid fertilizer as a substitute for chemical fertilizers using broken eggs as a resource and to evaluate the efficiency of the formulated fertilizer. To make the liquid fertilizer, the broken eggs and distilled water were mixed at ratios of 6 : 4 and 4 : 6. Then, effective micro-organisms (EM) and sugar were added, and the mixture was fermented. The temperature and electrical conductivity (EC) increased gradually with the fermentation while the pH decreased. When evaluated following the seed germination index method of the compost, it was found that the fertilizer matured 10 days after the beginning of the experiment. The growth experiment was conducted with lettuce in which the fermented liquid fertilizer was compared with a commercial liquid fertilizer. The 6 : 4 treatment produced plants with the densest fresh shoot and roots weighing 41.6 and 4.6 g, respectively. The number of leaves (12.3 per plant) was also the highest for the 6 : 4 treatment. Soil analysis showed that the soil pH was improved, and the soil organic matter was increased in the fermented liquid fertilizer treatment.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.6
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• pp.876-887
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• 2015

Dicer, an ribonuclease type III type endonuclease, is the key enzyme involved in biogenesis of microRNAs (miRNAs) and small interfering RNAs (siRNAs), and thus plays a critical role in RNA interference through post transcriptional regulation of gene expression. This enzyme has not been well studied in the Indian water buffalo, an important species known for disease resistance and high milk production. In this study, the primary coding sequence (5,778 bp) of bubaline dicer (GenBank: AB969677.1) was determined and the bubaline Dicer1 biocomputationally characterized to determine the phylogenetic signature among higher eukaryotes. The evolutionary tree revealed that all the transcript variants of Dicer1 belonging to a specific species were within the same node and the sequences belonging to primates, rodents and lagomorphs, avians and reptiles formed independent clusters. The bubaline dicer1 is closely related to that of cattle and other ruminants and significantly divergent from dicer of lower species such as tapeworm, sea urchin and fruit fly. Evolutionary divergence analysis conducted using MEGA6 software indicated that dicer has undergone purifying selection over the time. Seventeen divergent sequences, representing each of the families/taxa were selected to study the specific regions of positive vis-$\grave{a}$-vis negative selection using different models like single likelihood ancestor counting, fixed effects likelihood, and random effects likelihood of Datamonkey server. Comparative analysis of the domain structure revealed that Dicer1 is conserved across mammalian species while variation both in terms of length of Dicer enzyme and presence or absence of domain is evident in the lower organisms.

• The Plant Pathology Journal
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• v.20 no.2
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• pp.127-130
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• 2004

Grapevine leaf roll-associated virus 3 (GLRaV-3) is one of the most important viral diseases of grapevine in the world. In this study, GLRaV-3 Ampelovirus was identi-fied from grapevines in Korea by analyzing viral coat protein size, nucleotide, and amino acid sequences. The molecular weight of viral coat protein from virus-infected in vitro plantlets was determined by western blot using a commercial GLRaV-3 polyclonal antibody. Western blot analysis showed a coat protein of about 43 kDa. RT-PCR product of about 942 bp which encoded the coat protein (CP) gene was amplified with specific primers. When the viruses existed at low titers in the host plant, the dsRNA had very specific template in RT- PCR amplification of fruit tree viruses. Especially, small-scale dsRNA extraction method was very reliable and rapid. Sequence analysis revealed that the CP of the GLRaV-3 Ko consisted of 942 bp nucleotide, which encoded 314 amino acid residues. The CP gene of GLRaV-3 Ko had 98.9% nucleotide sequence and 98.7% amino acid sequence identities with earlier reported GLRaV-3. This is the first report on molecular assay of GLRaV-3 Ampelovirus identified from Korea. The GLRaV-3 Ko CP clone would be very useful for breeding of virus resistant grapevines.

• Korean Journal of Medicinal Crop Science
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• v.11 no.2
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• pp.122-126
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• 2003

This study was conducted to investigate plant growth characteristics and anatomical studies of Korean mistel (Viscum album var. coloratum (Kom.) Ohwi). Parasite position was upper part that has a plenty of sunshine area 5 to 10m apart from the ground, regardless host tree. Stem developed two branch stems from the each basal stem, leaf was grown from the end stems as opposite. Fruit has a light yellow color and berry size was 7 to 8 mm long diameter and it contains seed. Floral bud differentiation initiated with forming rounded dome and arch-shaped floral apex at the beginning stage, and produced heart-shaped sepal following the differentiation of leaf primordia. Floral bud formation processed centeral part first and following bilateral sides were developed in order. Leaf was thick and stoma and guard cell were observed from the two or three inner layers from the epidermis. Mistletoe penetrated cork cambium of host plant and was growing by rooting on xylem. Korean mistel connected to host plant through parasitic organ, haustorium structurally and physiologically.

• Journal of Digital Convergence
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• v.18 no.1
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• pp.199-209
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• 2020

This study was done to explore deeply the essence and meaning of gratitude experience of mothers who cares child with schizophrenia. This study was analysed through the use of the phenomenological method of Giorgi. Data was collected through in-depth interviews, observation, and field records with five motheres who cares child with schizophrenia. Data analysis revealed 18 sub-components, 5 components: 'I'm like fallen seed on barren soil without gratitude', 'gratitude coming into bud like sprout,' 'gratitude grown up into a tree through tolerance', 'gratitude bearing fruit of self-sufficiency and desire', and 'gratitude getting much richer with giving. The results from the study contribute to promoting understanding of the gratitude experience of mothers who cares child with schizophrenia and will be helpful for development of positive family nursing intervention.

• Korean journal of applied entomology
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• v.41 no.1
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• pp.33-41
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• 2002

The mirid bug damages on grapevine were observed not only on young shoot and leaves (decoloration, deforming, and perforation) but also on fruits (black spot, corky scar, and young fruit shattering). Among 159 fields surveyed throughout the country, 113 fields (71%) were damaged, and the damages were relatively severer in the chief producing districts of grape vine, such as Gimpo, Anseong, Naju, Gimcheon, Buyeo, Daejeon, Yesan than other districts where grapevine yards were not concentrated. The severest shoot damages were observed in two grape vine yards of Naju (85%) and Gimpo (65%) in 1999. Three species of virid bugs were recognized on grape vine tree, Apoygus spinolae (Meyer-Dur), Apolygus lucorum (Meyer-Dur), and Orthotylus (Melanotrichus) flavosparsus(Sahlberg). Among them, A. spinolae was dominant species in the number of specimens collected. A. spinolae were collected on 10 host plants including Vitis vinifera L., Prunus armeniaca var. ansu Max., and prunus serrulata var. spontanea(Max.) Wils. and etc., and A. lucorum were collected on 10 species of plants, including Artemisia iwayomogi Kitamura, Artemisia princeps var. orientalis(Pampan) Hara and etc. Both A. spinolae and A. lucorum distributed widely in Korean grapevine yards.

• Korean journal of applied entomology
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• v.50 no.4
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• pp.379-382
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• 2011

The damage rates of peach pyralid moth(Dichocrocis punctiferalis), which damages the chestnut fruits, were investigated annually from 1995 to 2010 according to year, region, and maturity. The damage rate by D. punctiferalis was the highest (34.5%) in 1998 and was the lowest (17.9%) in 2000. The pattern of the damage rate showed a repeating three-year cycle. There was a negative correlation ($-0.6261^*$) between rainfall and damage rate. There was a positive correlation ($0.5826^*$) between temperature and damage rate. The average damage rate of all surveyed regions was 22.2%. Of the surveyed areas, Hapcheon had the highest at 27% and Hamyang had the lowest at 15.0%. The damage rate of chestnut fruit depending on the maturity was 26.1% in an early maturing cultivar (Dantaek), and 19.3% and 21.1% in a late maturing cultivar (Eungi) and a medium maturing cultivar (Chukpa), respectively.

• Korean journal of applied entomology
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• v.51 no.4
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• pp.443-447
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• 2012

Clearwing moths (Lepidoptera: Sesiidae) are economically important pests of fruit trees, such as apple, peach, and grape in Korea. Larvae feed on the phloem and cambium within tree branches of host plants. In this study, the seasonal fluctuation in male catches of four clearwing moths, Synanthedon haitangvora, S. bicingulata, Nokona regalis, and Glossosphecia romanovi, were monitored with the respective sex pheromone traps in Suwon for three years. In apple orchards, S. haitangvora males were captured from May to October, with two peaks in early June to middle June and late August to early September. Similarly, S. bicingulata males were trapped from May to October, with two peaks in late May and late August to middle September in peach orchards. In vineyards, N. regalis males were captured from late May to middle June with one peak in late May to early June, while G. romanovi males were trapped from early June to late July with one peak in late June to early July.