• Clinical and Experimental Reproductive Medicine
• /
• 제30권3호
• /
• pp.241-248
• /
• 2003

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

• 한국추진공학회:학술대회논문집
• /
• 한국추진공학회 2004년도 제22회 춘계학술대회논문집
• /
• pp.327-334
• /
• 2004

Spectroscopic and electrostatic probe measurements were made to examine plasma characteristics with or without a metal plate for a 10-㎾-class direct-current arcjet Heat fluxes into the plate from the plasma were also evaluated with a Nickel slug and thermocouple arrangement. Ammonia and mixtures of nitrogen and hydrogen were used. The NH$_3$ and $N_2$+3H$_2$ plasmas in the nozzle and in the downstream plume without a plate were in thermodynamical nonequilibrium states. As a result, the H-atom electronic excitation temperature and the $N_2$ molecule-rotational excitation temperature intensively decreased downstream in the nozzle although the NH molecule-rotational excitation temperature did not show an axial decrease. Each temperature was kept in a small range in the plume without a plate except for the NH rotational temperature for NH$_3$ gas. On the other hand, as approaching the plate, the thermodynamical nonequilibrium plasma came to be a temperature-equilibrium one because the plasma flow tended to stagnate in front of the plate. The electron temperature had a small radial variation near the plate. Both the electron number density and the heat flux decreased radially outward, and an increase in H$_2$ mole fraction raised them at a constant radial position. In cases with NH$_3$ and $N_2$+3H$_2$ a large number of NH radical with a radially wide distribution was considered to cause a large amount of energy loss, i.e., frozen flow loss, for arcjet thrusters.

• 한국항공우주학회지
• /
• 제36권9호
• /
• pp.907-915
• /
• 2008

하이드라진(N2H4) 아크젯 추력기의 열화학 유동장 해석을 위한 전산유체해석을 수행하였다. 열복사와 전기장이 고려된 압축성 유동해석을 위해서 RANS 방정식을 수정하여 사용하였다. 로렌츠 힘과 Ohm 가열효과를 고려한 Maxwell 방정식이 유동방정식과 결합되어 전기 방전으로 인한 전기장해석을 위해 이용되었다. 아크젯 추력기 내부에서 유동장은 충분히 고온상태이기에 화학평형 해석이 이용되었으며, 광학 두께를 이용한 열복사 모델이 유동방정식에 적용되었다. 계산 결과들은 아크젯 추력기유동이 동결유동에 비해 추력은 180%증가되며, 비추력은 200%가까이 상승됨을 보여준다. 또한 유동장 해석 결과들은 아크젯 추력기 내부의 열적 물리적 특성에 대한 이해를 돕는다.

• Bulletin of the Korean Chemical Society
• /
• 제29권9호
• /
• pp.1711-1716
• /
• 2008

The reaction of copper(II) chloride with phenyl-N-[(pyridine-2-yl)methylene]methaneamide (ppmma) leads to a new $\mu$ -chloro bridged dimeric [Cu(ppmma)$Cl_2$]$_2$ complex, whereas a reaction of copper(II) bromide with ppmma affords a monomeric Cu(ppmma)$Br_2$ complex. Both complexes have been characterized by X-ray crystallography and electronic absorption spectroscopy. The crystal structural analysis of [Cu(ppmma)$Cl_2$]$_2$ shows that the two Cu(II) atoms are bridged by two chloride ligands, forming a dimeric copper(II) complex and the copper ion has a distorted square-pyramidal geometry ($\tau$ = 0.2). The dimer units are held through a strong intermolecular $\pi-\pi$ interactions between the nearest benzyl rings. On the other hand, Cu(ppmma)Br2 displayed a distorted square planar geometry with two types of strong intermolecular π-π interaction. EPR spectrum of [Cu(ppmma)$Cl_2$]$_2$ in frozen glas s at 77 K revealed an equilibrium between the mononuclear and binuclear species. The magnetic susceptibilities data of [Cu(ppmma)$Cl_2$]$_2$ and Cu(ppmma)$Br_2$ follow the Curie-Weiss law. No significant intermolecular magnetic interactions were examined in both complexes, and magnetic exchange interactions are discussed on the basis of the structural features.

• 농업생명과학연구
• /
• 제43권2호
• /
• pp.55-63
• /
• 2009

본 논문에서는 선행연구와 달리 냉장육 돼지고기와 냉동육 돼지고기에 대한 서로 다른 관세철폐 일정을 명시적으로 고려하여 한?미 FTA가 국내 돼지고기 시장에 미치는 영향을 분석하였다. 국산 돼지고기의 균형가격은 최소 2.69%에서 최대 15.96%까지 감소하고, 물량은 최소 1.35%에서 최대 5.6%까지 감소할 수 있는 것으로 시뮬레이션 된다. 또한 부위별로 보면 관세철폐 이행 이후 돼지고기의 도매가격은 kg당 최소 100원에서 최대 1300원까지 하락할 수 있는 것으로 분석된다. 이러한 분석결과는 한 EU의 협상결과나 DDA 협상 타결로 인한 다른 나라 돼지고기의 수입증가와 같은 돼지고기 시장의 다른 측면과 돼지고기가 타 육류와 가지는 대체관계 등은 고려하지 않은 것이기 때문에, 본 논문의 분석결과는 다른 모든 조건이 일정하다는 가정 하에 미국산 돼지고기의 수입가격이 하락한 상황이 가져오는 효과만을 분석했다는 전제하에 이해되어야 한다.

• 농업과학연구
• /
• 제49권4호
• /
• pp.949-961
• /
• 2022

The purpose of this study is to build an oyster outlook model. In particular, by limiting oyster items, it was designed as a partial equilibrium model based on a panel analysis of a fixed effect model on aquaculture facilities. The model was built with a dynamic ecological equation (DEEM) system that considers aquaculture and harvesting processes. As a result of the estimation of the initial aquaculture facilities based on the panel analysis, the elasticity of the remaining facility volume in the previous month was estimated to be 0.63. According to Nerlove's model, the adjustment coefficient was interpreted as 0.31 and the adjustment speed was analyzed to be very slow. Also, the relative income coefficient was estimated to be 2.41. In terms of elasticity, it was estimated as 0.08% in Gyeongnam, 0.32% in Jeonnam, and 1.98% in other regions. It was analyzed that the elasticity of relative income was accordingly higher in non-main production area. In case of the estimation of the monthly harvest facility volume, the elasticity of the remaining facility volume in the previous month was estimated as 0.53, and the elasticity of the farm-gate price was estimated as 0.23. Both fresh and chilled and frozen oysters' exports were estimated to be sensitive to fluctuations in domestic prices and exchange rates, while Japanese wholesale prices were estimated to be relatively low in sensitivity, especially to the exchange rate with Japan. In estimating the farm-gate price, the price elasticity coefficient of monthly production was estimated to be inelastic at 0.25.

• 분석과학
• /
• 제7권2호
• /
• pp.213-224
• /
• 1994

선택적으로 중수소를 치환시킨 싸이클로옥타논의 여러 동위원소 이성질체들을 합성하였다. 고유동위원소 효과에 의해 영향을 받는 $^{13}C$ NMR 화학적 이동값들을 각 이성질체에 대해 저온에서 계통적으로 관찰하였다. 특히 싸이클로옥타논이 선호하는 안정한 형태 이성질체인 클 boat-chair 형과 연관시켜 이 효과들을 논의하였다.