• Journal of Embryo Transfer
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• v.23 no.4
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• pp.275-281
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• 2008

The ability to preselect the sex of piglets is advantageous in the pig industry. The objective of this study was to examine the feasibility of using intracytoplasmic sperm injection (ICSI) with sorted spermatozoa to produce piglets with a preselected sex. Pig embryos were produced by ICSI of frozen X- and Y-sperm that had been separated by flow cytometry. The developmental competence of the embryos was investigated in vitro and in vivo. The populations of X- and Y-spermatozoa were 52.7% and 47.3%, respectively in our samples. The in vitro development of ICSI embryos was enhanced by longer of in vitro maturation of oocytes ($44{\sim}48\;h$ vs. $40{\sim}43\;h$). Their cleavage ($65{\sim}70%$) and blastocyst formation ($9{\sim}12%$) rates were not significantly different between male and female ICSI embryos, or between sorted and unsorted sperm-derived embryos. One pregnancy was established in a recipient that was transferred with 110 female ICSI embryos, but the pregnancy was terminated on Day 89 of gestation. Our results suggest that the separation X- and Y-spermatozoa by flow cytometric sorting can be a useful tool in combination with ICSI for the production of pig embryos and piglets of preselected sex.

• Journal of the Korean Society of Visualization
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• v.15 no.2
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• pp.26-32
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• 2017

Hypochlorous acid(HOCl) is a chemical that is a safe sanitizer and disinfectant approved by the Food and Drug Administration as a food additive, exhibiting strong sterilizing power with low effective chlorine concentration of pH 5.0-6.5 and effective chlorine concentration 10-80 ppm. To apply to fishery industries, we develope the HOCl ice for store or delivery of fishery products. However when HOCl is being frozen, the contained HOCl are expelled out from the ice due to the molecular structures of ice; there is no space to contain HOCl inside. To increase chlorine containing amount in ice, we develop the alginate particles containing HOCl which is bio comparable since alginate is a natural polymer extracted from the brown algae and it is widely used for drug delivery and containing substances, etc. We produce HOCl with water as base solution suppressing osmotic flow from fishery products, and mix it with the developed alginate particles and made HOCl-alginate ice and checked the remaining amount of HOCl. We measure the change of pH and chlorine concentration optimizing the best concentration of alginate particles. Finally, we produce the alginate particle HOCl ices with respect to the alginate's optimal concentration.

• Journal of the Korean Society of Propulsion Engineers
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• v.13 no.1
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• pp.34-42
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• 2009

High-speed air turbine handpieces have been used as a dental cutting tool in clinical dentistry for over 50 years, but little study has been reported on their performance analysis. Therefore, the effect of turbine blade shape on performance characteristics of dental air turbine handpiece were studied using CFD in this paper. Computations have been performed for five different positions of turbine blade by using frozen rotor method that is one of steady-state method. The characteristics of turbine blade for shapes and reflection angles were analyzed. As a result of the computation, torque is increased by increasing the reflection angle of turbine blade.

• Polymer(Korea)
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• v.34 no.3
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• pp.261-268
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• 2010

Phase change materials based on polypropylene blended with n-eicosane were studied in this paper. In addition, this paper reviews recent studies on the preparation of shape stabilized phase change materials (SSPCM), such as SSPCM from polypropylenes and n-eicosane, their basic properties and possible applications to latent heat storage. The preparation methods used were the melting method and absorption methods. Shape stabilized PCM(SSPCM) prepared for DSC, WAXD, FTIR spectroscopy, ARES, results of the analysis of shape stability heat capacity to improve were identified.

• Magazine of the Korean Society of Agricultural Engineers
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• v.31 no.2
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• pp.43-53
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• 1989

In this research programs, a series test was conducted to show the effects of freeze/thaw process on the various soil properties. The tests were carried out taken from the west sea shore of Korean peninsular and the west sea shore of Scotland, and their results are as follows; 1. There was a positive total heave in a freezing run, although water may he expelled for the sample initially. The water flow must he reverse' from expulsion to intake. 2. The confining pressure had an overriding influence on the heave and frost penetration, a sudden change of the axial strain at failure with strain rate was observed occuring at a strain rate between 10-5 and 10-6, and the initial friction angle of frozen clay was appeared zero. 3. There was shown a significant decrease in liquid limit of soil which was subjected to freeze/thaw process for the initial value of about 20% because of soil particles aggregation. 4. The cyclic freeze/thaw caused a sinificant reduction in shear strength and its thixotropic regain. The frozen/thawed soil exibited negative strength regain, particularly at high freeze/thaw cycles. 5. The freezing temperature greatly influenced on the failure strength of soils and this. Trend was more pronounced the lower the freezing temperature and shown the ductile failure with indistinct peaks.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.30 no.11 s.254
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• pp.1131-1138
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• 2006

Aerodynamic shape design of a partial admission turbine using CFD has been performed. Two step approaches are adopted in this study. Firstly, two-dimensional blade shape is optimized using CFD and genetic algorithm. Initially, the turbine cascade shape is represented by four design parameters. By controlling the design parameters as variables, the non-gradient search is analyzed for obtaining the maximum efficiency. The final two-dimensional blade proved to have a more blade power than the initial blade. Secondly, the three-dimensional CFD analysis including the nozzle, rotor and stator has been conducted. To avoid a heavy computational load due to an unsteady calculation, the frozen rotor method is implemented in steady calculation. The frozen rotor method can detect a variation of the flow-field dependent upon the blade's circumferential position relative to the nozzle. It gives a better idea of wake loss mechanism starting from the lip of the nozzle than the mixing plane concept. Finally, the combination of two and three dimensional design method of the partial admission turbine in this study has proven to be a robust tool in development phase.

• Fisheries and Aquatic Sciences
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• v.13 no.3
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• pp.231-235
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• 2010

The effects of various natural antioxidant sources on oxidation of olive flounder (Paralichthys olivaceus) and fish diet during storage was determined. Juvenile fish were distributed among 18 flow-through tanks (40 fish per tank). Six experimental diets were prepared in triplicate: control (CT), antitox (AT), green tea extract (GE), fig extract (FE), Haeroc product (HP) and by-product of green tea (BG). The experimental diets were stored at two temperatures: room temperature ($26.8^{\circ}C$) for 14 days and frozen ($-30^{\circ}C$) for 16 weeks. Thirty fish were sampled from each tank at the end of the 8-week feeding trial. Whole bodies of fish were homogenized and stored in a home freezer ($-9.6^{\circ}C$) for 24 weeks. Acid values (AVs) and peroxide values (POVs) of the diets and frozen fish during storage were monitored. AVs of the experimental diets tended to increase with the storage period except for that of the HP diet at room temperature. POVs from FE, CT, and BG diets peaked at day 7 and then decreased through the remainder of the experiment. AVs of the experimental diets and fish increased with time at $-30^{\circ}C$ and $-9.6^{\circ}C$. Results of this study show that by-products of green tea and Haeroc product seem to have potential as antioxidants in fish feed to inhibit oxidation of both the feed and fish during storage.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.30 no.1
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• pp.114-123
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• 2002

A supersonic rocket plum flowfield of kerosene/liquid-oxygen based propulsion system has been analysed using the Reynolds-averaged Navier-Stokes equations coupled with a 9-species 14-reaction finite-chemistry model. The result were compared with chemically frozen flow solution to investigate the effect of finite-rate chemistry on the plume flowfield. The computations were performed using a commercial CFD software, FLUENT 5. The finite-rate chemistry solution exhibited higher temperature caused by the reactions within the nozzle. All the chemical reactions within the plum were dominated only in the shear layer and behind the barrel shock reflection region where the temperatures are high and the effect of finite-rate chemical reactions on the flowfield was found to be insignificant. However, the present plume computation including the finite-rate chemical reaction within the plume has revealed major reactions occurring in the plum and their reaction mechanisms.

• 한국전산유체공학회:학술대회논문집
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• 1999.11a
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• pp.71-76
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• 1999

The base flow regions of a three-body sounding rocket containing multiple exhaust plumes were numerically investigated in three dimensions for a free stream Mach number of 2.7 at flight altitude 18.5 km. The flowfields were calculated using the full compressible Navier-Stokes equations with an one-equation turbulence model of Baldwin-Earth. The present calculations were executed based upon a chemically frozen, single perfect gas model assumption. Due to the symmetry of the three-body rocket of each single nozzle, only one fourth of the computational domain was considered for the analysis. The results indicate that a babe heating effect is not considerable due to the small expansion of the plumes. In the base, however, a low speed recirculating flow dominates the region.

• 한국전산유체공학회:학술대회논문집
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• 2008.03b
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• pp.152-155
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• 2008

In this paper, the effect of modification of geometric variables on the performance of a centrifugal compressor blade has been studied numerically. The compressor contains six main blades and six splitter blades. Reynolds averaged Navier-Stokes (RANS) equations with shear stress turbulence (SST) model are discretized by finite volume approximations and solved on hexahedral grids for flow analysis. The design variables from blade lean angle at tip and middle of the blade have been modified. The isentropic blade efficiency and pressure have been predicted with the variation of the variables. Frozen rotor simulation is performed and adiabatic wall condition has been used. One of the six blades of compressor has been used for simulation to reduce the computational load. Optimum number of meshes has been selected by grid-dependency test, and this is used for all the simulations with changing geometric variables. The detailed flow analysis results have been reported as well as the effects of the variables.