• Journal of Microbiology and Biotechnology
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• 제31권2호
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• pp.240-249
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• 2021

Phenotypic plasticity is a rapid response mechanism that enables organisms to acclimate and survive in changing environments. The Chinese mitten crab (Eriocheir sinensis) survives and thrives in different and even introduced habitats, thereby indicating its high phenotypic plasticity. However, the underpinnings of the high plasticity of E. sinensis have not been comprehensively investigated. In this study, we conducted an integrated gut microbiome and muscle metabolome analysis on E. sinensis collected from three different environments, namely, an artificial pond, Yangcheng Lake, and Yangtze River, to uncover the mechanism of its high phenotypic plasticity. Our study presents three divergent gut microbiotas and muscle metabolic profiles that corresponded to the three environments. The composition and diversity of the core gut microbiota (Proteobacteria, Bacteroidetes, Tenericutes, and Firmicutes) varied among the different environments while the metabolites associated with amino acids, fatty acids, and terpene compounds displayed significantly different concentration levels. The results revealed that the gut microbiome community and muscle metabolome were significantly affected by the habitat environments. Our findings indicate the high phenotypic plasticity in terms of gut microbiome and muscle metabolome of E. sinensis when it faces environmental changes, which would also facilitate its acclimation and adaptation to diverse and even introduced environments.

• 한국발생생물학회지:발생과생식
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• 제13권2호
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• pp.97-103
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• 2009

Genomic DNA samples isolated from four geographical freshwater crab (Eriocheir sinensis) populations collected in the inland of the Korean Peninsula (Gunsan, Paju, and Nampo) and a Chinese site, were used for PCR amplification. Seven decamer primers generated 19 specific loci (19/243 loci, 7.81%) in the Gunsan population, 32 (32/215 loci, 14.88%) in the Paju population, 19 (19/231 loci, 8.23%) in the Nampo population and 62 (62/340 loci, 18.24%) in a Chinese population. The average 8.9 specific loci exhibited inter-individual-specific characteristics, thus revealing DNA polymorphisms in the Chinese population. The number of unique shared loci to each population and number of shared loci by the four populations were generated by molecular analysis using seven primers in four populations. 35 unique shared loci to each population, with an average of 5.0 per primer, were observed in the Gunsan population, and 50 loci, with an average of 7.1 per primer, were observed in the Chinese population. The hierarchical dendrogram indicates three main branches: cluster 1 (GUNSAN 01$\sim$GUNSAN 05, PAJU 06$\sim$PAJU 10 and NAMPO 11$\sim$NAMPO 15) and cluster 2 (CHINESE 16, 17, 18, 19 and 20). Conclusively individual no. 20 of the PAJU 10 freshwater crab was most distantly related to CHINESE no. 20 (genetic distance = 0.667). Taken together, these results demonstrate the potential of RAPD analysis to identify diagnostic markers for the identification of four freshwater crab populations.

• 환경생물
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• 제30권1호
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• pp.26-30
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• 2012

본 연구는 금강하구둑과 관련하여 금강에 서식하는 참게($Eriocheir$ $sinensis$)의 이동과 산란을 알아보기 위해 2007년 5월부터 2009년 1월까지 금강 수계에서 조사하였다. 참게의 유생은 5월에 기수역에서 채집되었으나, 이후 치해로 변태하여 소상하는 개체들은 채집되지 않았다. 산란을 위해 참게들이 금강하구에 도달하는 시기는 9월 초순이었고, 3월부터 4월 사이에 산란을 하는 것으로 확인되었다. 연구 결과 기수에서 부화한 참게의 유생이 치해로 변태하여 담수로 이동하는 통로를 만들어 준다면, 금강 수계의 자연산 참게 복원이 제한적이나마 가능할 것으로 판단된다.

• 한국발생생물학회지:발생과생식
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• 제12권3호
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• pp.231-241
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• 2008

본 실험은 동남참게, Eriocheir japonicus의 명확한 생식년주기를 밝히기 위하여, 그들의 자연 서식처인 섬진강에서 채집한 성체 참게의 생식소 조직의 계절적인 변화 그리고, 암 참게 생식소의 성숙과 산란에 미치는 환경요인 즉, 온도, 광주기 그리고, 염분 농도에 대하여 조사하였다. 암 참게의 생식소 발달과 GSI의 계절적 변화를 기초로하여 생식년주기를 다음과 같이 4단계로 구분하였다: 난황형성전기$9{\sim}10$월), GSI는 낮았으며, 난소내에는 난황형성전기와 감수분열전기의 난모세포들을 가지고 있었다; 성숙기(11월$\sim$다음해 3월), GSI는 점차적로 증가하여 난모세포내에는 난황구들이 축적되었다;에는 영향을 받지 않았다. 광주기 조건(12L12D, 9L15D)에 관계없이, $10^{\circ}C$ 실험군의 암 참게보다, $18^{\circ}C$ 실험군의 암 참게의 GSI가 더욱더 증가하였다. 그리고, $26^{\circ}C$ 실험군에서 두 개의 광주기 조건(12L12D, 9L15D)에서 암 참게의 GSI는 변화하지 않았으며, 난소내에는 난황이 축적되고 있는 난모세포들은 없었다. 산란은 수온과 염분 농도에 의하여 많은 영향을 받으며, 난황형성중인 암 참게는 두 달간 사육하여도, $10^{\circ}C$에서는 전혀 산란을 하지 않았으며, $18^{\circ}C$와 $26^{\circ}C$에서 사육한 암 참게의 반 이상은 염분 농도 9.6%o와 19.2%o의 조건하에서 산란하였다. 그러나, 염분 농도 0.0%o의 조건에서는 산란한 암 참게가 한 마리도 없었다.

• 한국수산과학회지
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• 제51권1호
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• pp.47-53
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• 2018

The presence of pathogens in wild fish is a potential threat to the fish being raised nearby fish farm. Surveillance of these pathogens in the wild is, thus, highly important to keep fish in the farm safe from serious communicable diseases. Fish and crustacean were sampled 4 times at Soyang Lake in 2016. Pathogens for five reportable communicable diseases including KHVD (koi herpesvirus disease), SVC (spring viraemia of carp), EUS (epizootic ulcerative syndrome) and WSD (white spot disease), and parasites were investigated. In Soyang Lake, pale chub Zacco platypus was the most abundant. Forty seven pale chub, 8 Korea piscivorous chub Opsarichthys uncirostris, 3 oily shiner Sarcocheilichthys variegatus wakiyae and 2 crucian carp Cyprinus carassius were investigated for detection of target diseases of fish. Seventeen Caridina denticulata denticulata, 12 Oriental river prawn Macrobrachium nipponense and 1 Chinese mitten crab Eriocheir sinensis were used for detection of WSD. As the result, reportable communicable diseases were not detected in all the freshwater animals. Dactylogyrus was detected in Korea piscivorous chub. Copepoda was also detected in pale chub and Korea piscivorous chub. Metacercaria was detected in the pale chub sampled in September and October. Those were not metacercaria of liver fluke Clonorchis sinensis.

• 한국수산과학회지
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• 제51권3호
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• pp.248-253
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• 2018

Freshwater farms are primarily located adjacent to rivers and lakes, facilitating the introduction and spread of pathogens into natural systems. Therefore, it is necessary to continuously monitor natural aquatic organisms, the breeding environment, and infection rates by pathogenic organisms. Fish and crustaceans were sampled 4 times in the Geum River estuary in 2016. The samples were analyzed for the presence of pathogens for reportable communicable diseases, including KHVD (koi herpesvirus disease), SVC (spring viraemia of carp), EUS (epizootic ulcerative syndrome) and WSD (white spot disease); parasite abundance was also examined. The dominant fish species were deep body bitterling Acanthorhodes macropterus (21.4%), followed by skygager Erythroculter erythropterus (12.7%). For crustaceans, Palaemon paucidens and Chinese mitten crab Eriocheir sinensis were dominant. Sixty fish and 36 crustacean species were examined for reportable communicable diseases. When using a specific primer set for each disease, PCR analysis did not detect any reportable communicable diseases in the samples. Some instances of Dactylogyrus, copepods, nematodes and metacercaria were detected. However, the PCR results indicated that the metacercaria were not Clonorchis sinensis.

• 한국어병학회지
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• 제26권2호
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• pp.99-110
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• 2013

수산종묘는 인류에게 중요한 동물성 단백질을 제공할 뿐만 아니라, 방류를 통한 수산자원 관리의 측면에서도 매우 중요하다. 수산자원의 증강을 목적으로 2009년에는 33품종, 2010년에는 44품종, 2011년에는 43품종, 2012년에는 46품종에 대해 수산생물전염병에 대한 감염 여부를 조사하였다. 검사품종 중에서 해면품종으로는 전복이 가장 많았으며, 그 다음 해삼, 넙치, 조피볼락, 꽃게 순으로 나타났다. 내수면품종 중에서는 붕어가 가장 많았으며, 그 다음으로 쏘가리, 대농갱이, 다슬기, 참게의 순으로 나타났다. 감성돔, 돌돔, 붉은쏨뱅이, 뱀장어는 4년 동안 지속적으로 검사 횟수가 감소하였다. 숭어에 대해서는 2009년에만 전염병 검사가 수행되었다. 8개의 검사 항목에 대해 총 8,476건의 검사가 실시되었으며, 56건 (0.67%)에서 koi herpesvirus, white spot syndrome virus, red sea bream iridovirus 또는 viral haemorrhagic septicemia virus와 같은 병원체가 검출되어 불합격 처리되었다.