• Title/Summary/Keyword: Frequency Doubling

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High Frequency Production of Doubled-haploid Plants by Colchicine Application in Anther Cultures of Rice (벼 약배양에서 콜히친이 식물체 배수성에 미치는 영양)

  • 권용삼;이형규;박규환;손재근
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.1
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    • pp.37-40
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    • 2001
  • To optimize the in uitro chromosome doubling procedure in anther cultures of rice, anthers were cultured on callus induction medium with 0.001 to 0.1 mg/L colchicine for 30 days. The addition of colchicine slightly reduced callus formation and plant regeneration in comparison to the colchicine-free medium (control medium). This reduction was greater with higher concentration colchicine. Microspore-derived rice plants in control medium were found to be mainly haploid (50 to 58.8%) and doubled-haploid (31 to 40%) in anther culture of 3 Japonica and 1 Tonsil type cultivars. The application of 0.001 mg/L colchicine was increased to 54.3∼60.0% in the frequency of fertile doubled-haploid plants. These results indicate that the addition of colchicine to the callus induction medium is an efficient means to obtain doubled-haploid plants in anther cultures of rice.

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A Study on a KTP Crystal Laser System for a Cancer Using P.D.T. (KTP 크리스탈을 이용한 PDT용 레이저 시스템 개발)

  • Kim, Byoung-Mun;Nam, Hyo-Duk;Kim, Byoung-Chul
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2004.07b
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    • pp.631-634
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    • 2004
  • The method that exists in Photodynamic Therapy uses Photosensibility drug strongly Influencing tumour accumulation together with photochemical laser effect and makes the structure of tumour be localized and become extinct. The intracavity transformation of the Nd :YAP main radiation 1079 nm was Raman converted in barium nitrate crystal and the Stokes frequency (1216 nm) was doubled using KTP or RTA crystals. The LiF or Cr:YAG crystals are used for the Q-switch. The radiation Parameters were obtained at 100 Hz pump repetition frequency. The average power at 608 nm radiation with LiF and KTP was 700 mW at multi-mode generation. The 3-6 single 10-15 ns pulses were generated during one cycle of pumping. The doubling efficiency with RTA was two times more than with KTP. The cells of Ehrlich adenocarcinoma (0.1 ml) were i.m. implanted in hind thighs of ICR white non-imbred mice. The cells were preliminarily diluted in medium 199 in the ratio of 1 to 5. HpD was intravenous administered in a dose of 10 mg/kg. The left clean-shaven hind leg was irradiated with laser light 21-27 hours after the administration of the preparation. The right non-Irradiated leg of each animal served as a control. The animals with the transplanted tumor that were not injected with HpD sewed as a control to estimate the complex effect (HpD+ irradiation). Before the administration of HpD and on 3 and 4 days after irradiation the tumor size was measured and the percent of the tumor growth inhibition was calculated. The results of animal treatments has shown high efficiency of PDT method for cancer treatment by means 0.608 m high power pulse solid state laser.

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Effects of Gelling Agents and Growth Regulation on Rice Anther Culture (배지 응고제와 생장조절제가 벼 약배양에 미치는 영향)

  • 이중호;이승엽
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.1
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    • pp.35-39
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    • 1995
  • In order to investigate the effects of gelling agent on rice anther culture, anthers of rice (Japonica cv Daecheongbyeo) were cultured on N$_{6}$ media supplemented with 0.8, 1.2 or 1.6% Junsei agar and 05, 0.4, 0.6, 0.8 or 1.0% Gelrite (Phytagel, Sigma). On Junsei agar media, the frequency of callus induction was decreased in proportion to agar concentration. The frequency of callus induction was more increased as 67.6% and 54.8% in media containing 0.4 and 0.6% Gelrite than in agar media. The frequency of plant regeneration and spontaneous doubled-diploid was directly proportional to Junsei agar and Gelrite concentration. The number of green and spontaneous doubled diploid plant was highest on 0.6% Gelrite medium. In order to optimize the concentration of growth regulators for the callus induction medium containing 0.6% Gelrite, anthers were cultured on N$_{6}$ media supplemented with 2mg/L NAA, 2 mg/L 2,4-D, 1mg/L NAA and 1mg/L 2, 4-D, or 1mg/L NAA, 1mg/L 2,4-D and 0.5mg/L kinetin. The maximum frequency of callus induction and plant regeneration was obtained from the medium supplemented with 2 mg/L NAA and 0.6% Gelrite. In conclusion the induction of embryogenic callus, the frequency of plant regeneration and in vivo chromosome doubling was more effective in Gelrite media than in Junsei agar media.dia.

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A Multipath Delay Time Detection Method For $\frac{\pi}{4}$ Shift QPSK Modulation Under The Frequency Selective Fading Environment (주파수 선택성 페이딩 환경하에서 $\frac{\pi}{4}$ shift QPSK 변조방식에 대한 다중파의 시간지역 검출법 제안)

  • 조병진;김대영
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.16 no.10
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    • pp.941-950
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    • 1991
  • channel is severely degraded by multipath delay time spread. In this paper. We propose a simple multipath delay time detection method, which has a merit of in serviceable, yet simple H/W realizability for $\pi/4$ shift QPSK by detecting cross channel interference. A $\pi/4$ shift QPSK signal originally has quadrature channel(Q-ch) component. Thus in order to measure CCI between in-phase channel(I-ch) and quadrature channel(Q-ch), which closely related to multipath delay time, Frequency doubling scheme(frequency doubler) and differential detector is proposed, which makes $\pi/4$ shift QPSK signal look like BPSK and also makes it possible for CCI to be detected at I-ch detector output. To get an information from time varying I-ch output signal under the multipath lading environment, a method for obtaining the mean of the absolute value$(V_{MABS}(t))$ and another one for obtaining the root mean square value$(V_{RMS}(t))$ of CCI are proposed. Furthermore, a relationship between delay spread and CCI is also analyzed. In order to confirm theoretical results, computer simulation has been carried out under the quasi-static and Reyleigh distributed two ray multipath fading environments. A fairly good result was obtained. However it was also shown that this method is sensitive to bandwidth restriction to some extent. In addition, some idea for a simple hardware realization for the frequency doubler are given.

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$KTiOPO_4 (KTP)$ Single Crystal Growth by TSSG Technique (TSSG법에 의한 $KTiOPO_4 (KTP)$ 단결정 육성)

  • 김정환;강진기
    • Korean Journal of Crystallography
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    • v.3 no.1
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    • pp.37-43
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    • 1992
  • KTiOP04 is a nonlinear optical crystal which is most widely used for frequency doubling of the radiation of Nd : YAG laser. In the experiment, sin ale crystals of KTiOP04 were grown by TSSG technique using 3K2W04·P2O5 flux. Low temperature gradient furnace suitable for KTP single crystal growth was used. Seed crystal was placed at the surface of the solution for the purpose of better observation of the growing crystals and the possibility of diameter control. Solution included 66.7mol% KTiOP04 for all experiments and its saturation temperature was 1020℃. The conditions of single crystal growth were as follows: cooling rate 0.2℃/h, crystal rotation rate 50rpm, c -axis seed. Using these conditions, single crystals up to 23 ×25×25mm3 have been groan from about 100cc solution. We have also observed a change in the crystal growth habit which resulted in the formation of large (201) faces and small (100) faces. And some crystals have (101) faces.

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Liquid crystal effects on poling behaviour of NLO chromophore dispersed in organically modified sol-gel materials (유/무기 졸-겔 재료에 비선형광학 물질의 배향특성에 대한 액정효과)

  • Baek, In-Chan;Seok, Sang-Il;Jin, Moon-Young;Lee, Chang-Jin
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2003.11a
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    • pp.132-132
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    • 2003
  • Second-order nonlinear optical(NLO) materials have been extensively studied for applications in photonic devices, such as frequency doubling and electro-optical(EO) modulation, because of their large optical nonlinearity, excellent processibility, low dielectric constant, and high laser damage thresholds. The poling behaviour of NLO chromophore in organic/inorganic matrixes showed the randomization of poled NLO chromophore in the absence of poling Held. The liquid crystal molecules in a droplet showed a long-range orientational order along a director. Therefore, liquid crystal effects on poling behaviour of NLO chromophore dispersed in organically modified inorganic sol-gel materials were investigated. Using sol-gel process for the development of NLO material has received increasing attention, Organically modifked inorganic NLO sol-Eel materials are obtained via incorporation of the organic NLO active chromophore into an alkoxysilane based inorganic network. One of the most important thing in this works was that tetraethoxysilane(TEOS) and methyltrimathoxysilane(HTMS) were used as precursor followed by hydrolysis and condensation without using any acidic catalyst during the process. The NLO chromophores in the liquid crystal nanodomains were well mixed with I/O hybrid matrix, deposited on transparent ITO-coated glasses. The poling behaviour of liquid crystal effects of NLO chromophore dispersed in I/O hybrid matrix were investigated by UV-vis spectroscopy. Size distribution and morphology of the NLO chromophores doped in the liquid crystal nanodomains dispersed in I/O hybrid matrix were investigated by SEM.

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Phenomenology of nonlinear aeroelastic responses of highly deformable joined wings

  • Cavallaro, Rauno;Iannelli, Andrea;Demasi, Luciano;Razon, Alan M.
    • Advances in aircraft and spacecraft science
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    • v.2 no.2
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    • pp.125-168
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    • 2015
  • Dynamic aeroelastic behavior of structurally nonlinear Joined Wings is presented. Three configurations, two characterized by a different location of the joint and one presenting a direct connection between the two wings (SensorCraft-like layout) are investigated. The snap-divergence is studied from a dynamic perspective in order to assess the real response of the configuration. The investigations also focus on the flutter occurrence (critical state) and postcritical phenomena. Limit Cycle Oscillations (LCOs) are observed, possibly followed by a loss of periodicity of the solution as speed is further increased. In some cases, it is also possible to ascertain the presence of period doubling (flip-) bifurcations. Differences between flutter (Hopf's bifurcation) speed evaluated with linear and nonlinear analyses are discussed in depth in order to understand if a linear (and thus computationally less intense) representation provides an acceptable estimate of the instability properties. Both frequency- and time-domain approaches are compared. Moreover, aerodynamic solvers based on the potential flow are critically examined. In particular, it is assessed in what measure more sophisticated aerodynamic and interface models impact the aeroelastic predictions. When the use of the tools gives different results, a physical interpretation of the leading mechanism generating the mismatch is provided. In particular, for PrandtlPlane-like configurations the aeroelastic response is very sensitive to the wake's shape. As a consequence, it is suggested that a more sophisticate modeling of the wake positively impacts the reliability of aerodynamic and aeroelastic analysis. For SensorCraft-like configurations some LCOs are characterized by a non-synchronous motion of the inner and outer portion of the lower wing: the wing's tip exhibits a small oscillation during the descending or ascending phase, whereas the mid-span station describes a sinusoidal-like trajectory in the time-domain.

Sea Ice Extents and global warming in Okhotsk Sea and surrounding Ocean - sea ice concentration using airborne microwave radiometer -

  • Nishio, Fumihiko
    • Proceedings of the KSRS Conference
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    • 1998.09a
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    • pp.76-82
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    • 1998
  • Increase of greenhouse gas due to $CO_2$ and CH$_4$ gases would cause the global warming in the atmosphere. According to the global circulation model, it is pointed out in the Okhotsk Sea that the large increase of atmospheric temperature might be occurredin this region by global warming due to the doubling of greenhouse effectgases. Therefore, it is very important to monitor the sea ice extents in the Okhotsk Sea. To improve the sea ice extents and concentration with more highly accuracy, the field experiments have begun to comparewith Airborne Microwave Radiometer (AMR) and video images installed on the aircraft (Beach-200). The sea ice concentration is generally proportional to the brightness temperature and accurate retrieval of sea ice concentration from the brightness temperature is important because of the sensitivity of multi-channel data with the amount of open water in the sea ice pack. During the field experiments of airborned AMR the multi-frequency data suggest that the sea ice concentration is slightly dependending on the sea ice types since the brightness temperature is different between the thin and small piece of sea ice floes, and a large ice flow with different surface signatures. On the basis of classification of two sea ice types, it is cleary distinguished between the thin ice and the large ice floe in the scatter plot of 36.5 and 89.0GHz, but it does not become to make clear of the scatter plot of 18.7 and 36.5GHz Two algorithms that have been used for deriving sea ice concentrations from airbomed multi-channel data are compared. One is the NASA Team Algorithm and the other is the Bootstrap Algorithm. Intrercomparison on both algorithms with the airborned data and sea ice concentration derived from video images bas shown that the Bootstrap Algorithm is more consistent with the binary maps of video images.

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VLSI Architecture for High Speed Implementation of Elliptic Curve Cryptographic Systems (타원곡선 암호 시스템의 고속 구현을 위한 VLSI 구조)

  • Kim, Chang-Hoon
    • The KIPS Transactions:PartC
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    • v.15C no.2
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    • pp.133-140
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    • 2008
  • In this paper, we propose a high performance elliptic curve cryptographic processor over $GF(2^{163})$. The proposed architecture is based on a modified Lopez-Dahab elliptic curve point multiplication algorithm and uses Gaussian normal basis for $GF(2^{163})$ field arithmetic. To achieve a high throughput rates, we design two new word-level arithmetic units over $GF(2^{163})$ and derive a parallelized elliptic curve point doubling and point addition algorithm with uniform addressing based on the Lopez-Dahab method. We implement our design using Xilinx XC4VLX80 FPGA device which uses 24,263 slices and has a maximum frequency of 143MHz. Our design is roughly 4.8 times faster with 2 times increased hardware complexity compared with the previous hardware implementation proposed by Shu. et. al. Therefore, the proposed elliptic curve cryptographic processor is well suited to elliptic curve cryptosystems requiring high throughput rates such as network processors and web servers.

A study on the lasing characteristics of diode-pumped, single-mode Nd:YVO4 green laser (다이오드 여기 Nd:YVO4 단일모드 녹색광 레이저의 출력 특성 연구)

  • 이용우;이종훈
    • Korean Journal of Optics and Photonics
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    • v.14 no.3
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    • pp.292-297
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    • 2003
  • A diode-pumped single-mode Nd:YV $O_4$ green laser was developed. Frequency doubling of the laser was achieved by using an intracavity KTP generated green beam (532 nm). By comparing the diode laser spectrum with absorption spectrum of the Nd:YV $O_4$ crystal, we found optimal operating temperature of the diode laser. From output power measured for various mirror curvatures and cavity lengths, we found the optimal matching of TE $M_{00}$ mode with the pump beam gives the best efficiency. When the pump power was 1.9 W, 80 ㎽ of TE $M_{00}$ mode green beam was obtained. We tried to get a single longitudinal mode lasing as the fluctuation of the laser power was caused by the shift of longitudinal modes and the beating between the modes. We tested the intracavity etalon method and birefringent filter method for single mode operation. The etalon method resulted in the best single mode output power of 60 ㎽. The power fluctuation of the single-mode laser was reduced to 1/10 of that of the multi-mode laser.