• Korean Journal of Food Science and Technology
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• v.40 no.1
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• pp.115-117
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• 2008

Liposomes are artificial membranes prepared by phospholipid. In this study, liposomes were prepared by dehydration-rehydration method, and then nano-sized by sonication and pressure. The sizes of the prepared multilamellar vesicles (MLV) were greater than 10 μm. Sonication with a tip-type sonifier or pressurization with a French press on MLV were carried out to reduce size. Sonication with an output of 112.5 W for 10 min on MLV resulted in sizes less than 450 nm. French press with 6,000 psi of pressure was able to manufacture liposomes of approximately 100 nm uniformly. Also, the sonication or pressure clarified the color of the liposome solutions. The results indicate that sonication and pressure via French press can be applied to obtain nano-sized liposomes.

• Structural Engineering and Mechanics
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• v.31 no.4
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• pp.439-451
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• 2009

The paper deals with the problem related to the modelling of riveted assemblies for crashworthiness analysis of full-scale complete aircraft structures. Comparisons between experiments and standard FE computations on high-energy accidental situations onto aluminium riveted panels show that macroscopic plastic strains are not sufficiently localised in the FE shells connected to rivet elements. The main reason is related to the structural embrittlement caused by holes, which are currently not modelled. Consequently, standard displacement FE models do not succeed in initialising and propagating the rupture in sheet metal plates and along rivet rows as observed in the experiments. However, the literature survey show that it is possible to formulate super-elements featuring defects that both give accurate singular strain fields and are compatible with standard displacement finite elements. These super-elements can be related to the displacement model of the hybrid-Trefftz principle of the finite element method, which is a kind of domain decomposition method. A feature of hybrid-Trefftz finite elements is that they are mainly used for elastic computations. It is thus proposed to investigate the possibility of formulating a hybrid displacement finite element, including the effects of a hole, dedicated to crashworthiness analysis of full-scale aeronautic structures.

• Geomechanics and Engineering
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• v.33 no.1
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• pp.65-75
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• 2023

Recycled concrete aggregate (RCA) is widely used as a construction material in road construction, concrete structures, embankments, etc. However, it has been reported that calcite (CaCO₃) precipitation from RCA can be a cause of clogging when used in drainage applications. An accelerated calcite precipitation (ACP) procedure has been devised to evaluate the long-term geochemical performance of RCA in subsurface drainage systems. While the ACP procedure was useful for the French Drain application, there remained opportunities for improvement. In this study, key factors that control the formation of calcite precipitation were quantitatively evaluated, and the results were used to improve the current prototype ACP method. A laboratory parametric study was carried out by investigating the effects of reaction temperature and time on the formation of calcite precipitation of RCA, with determining an optimum reaction temperature and time which maximizes calcite precipitation. The improved ACP procedure was then applied to RCA samples that were graded for Type I Underdrain application, to compare the calcite precipitation. Two key findings are (1) that calcite precipitation can be maximized with the optimum heating temperature (75℃) and time (17 hours), and (2) the potential for calcite precipitation from RCA is not as significant as for limestone. With the improved ACP procedure, the total amount of calcite precipitation from RCAs within the life cycle of a drain system can be determined when RCAs from different sources are used as pipe backfill materials in a drain system.

• Steel and Composite Structures
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• v.3 no.6
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• pp.383-401
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• 2003

Two new formulae for the design of beam-columns at room temperature have been proposed into Eurocode 3, prEN 1993-1-1 (2002), and are the result of great efforts made by two working groups that followed different approaches, a French-Belgian team and an Austrian-German one. Under fire conditions the prEN 1993-1-2 (structural fire design) presents formulae, for the design of beam-columns based on the prENV 1993-1-1 (1992). In order to study the possibility of having, in part 1-1 and part 1-2 of the Eurocode 3, the same approach, a numerical research was made using the finite element program SAFIR, developed at the University of Liege for the study of structures subjected to fire.

• Steel and Composite Structures
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• v.17 no.2
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• pp.185-198
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• 2014

Carbon-manganese steel A42 (French standards) is used in steam generator pipes of nuclear center and subject to low cycle fatigue (LCF) loads. In order to obtain the material LCF behavior, the tests are implemented in a hydraulic fatigue machine. The LCF plastic deformation and cyclic stress in macroscope have been influenced by the accumulated low cycle fatigue damage. The constitutive kinematic and isotropic hardening modeling is modified with coupling fatigue damage to describe the fatigue behavior. The improved model seems to be good agreement with the test results.

• Preventive Nutrition and Food Science
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• v.5 no.2
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• pp.81-85
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• 2000

Bromelain has been used as a meat-tenderizing agent in food processing. To increase the availability of bromelain, microencapsulation into liposome was carried out by the dehydration and rehydration method. Small unilamellar vesicles prepared by sonication treatment showed higher encapsulation efficiency (EE) than by the French press method. In the preparation of liposome, the effect of pH and centrifugal force on EE was also investigated and it showed a higher EE at acidic pH than at alkaline pH with centrifugation at 80, 000$\times$g. The actual EEs except unencapsulated bromelain which bound on the outside surface of liposome by electrostatic interaction also were investigated, and the optimal EE was at pH 4.6, at 0.6 of a ratio of bromelain to phosholipid(18.2%, w/w). Release of bromelain from liposomes was stimulated as the temperature increased at neutral pH.

• Steel and Composite Structures
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• v.34 no.3
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• pp.441-452
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• 2020

Within the French CIFRE research project COMINO, an innovative type of composite beam was developed for buildings that need fire resistance with no additional supports in construction stage. The developed solution is composed of a steel U-shaped beam acting as a formwork in construction stage for a reinforced concrete part that provides the fire resistance. In the exploitation stage, the steel and the reinforced concrete are acting together as a composite beam. This paper presents the investigation made on the load bearing capacity of this new developed steel-concrete composite section. A full-scale test has been carried out at the Laboratory of Structural Engineering of the University of Luxembourg. The paper presents the configuration of the specimen, the fabrication process and the obtained test results. The beam behaved compositely and exhibited high ductility and bending resistance. The shear connection in the tension zone was effective. The beam failed by a separation between the slab and the beam at high deformations, excessive shear forces conducted to a failure of the stirrups in this zone. The test results are then compared with good agreement to analytical methods of design based on EN 1994 and design guidelines are given.

• Korean Journal of Microbiology
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• v.23 no.1
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• pp.13-19
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• 1985

Acc I endonuclease has been isolated from 300g (wet weight) cells of Acinetobacter calcoaceticus. The cells were broken by using French press at 20, 000p.s.i. After ammonium sulfate fractionation, the enzyme was further purified by heparin agarose, DEAE-sephades, Affi.-gel Blue, phosphocellulose, and hydroxylapatite column chromatography. The purified Acc I endonudlease has a single polypeptide species and its subunit molecular weight was 45,000 ${\pm}$ 1,000 daltons as judged by 10% SDS-polyacrylamide gel electrophoresis. The isolated enzyme was essentially free of contaminating nucleases as judged by homochromatography by using a $^{32}P-labeled$ oligonucleotide. The enzyme showed maximum activity at pH values between 8.0 and 11.0 and in the presence of $MgCl_2$. Acc I endonuclease was maximally active in the absence of NaCl and was completely inhibited at 200 mM NaCl.

• Advances in Computational Design
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• v.4 no.1
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• pp.1-13
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• 2019

Recent generations of smartphones offer accelerometer sensors as a standard feature. While this has led to the development of a number of related applications (apps), there has been no study on their comparative or individual performance against a benchmark. This paper investigates the comparative performance of a number of smartphone accelerometer apps amongst themselves and to a calibrated benchmark accelerometer. A total of 12 apps were selected for testing out of 90 following an initial review. The selected apps were subjected to sinusoidal vibration testing of varying frequency and the response of each compared against the calibrated baseline accelerometer. The performance of apps was quantified using analysis of variance (ANOVA) and test of significance was carried out. The apps were then compared for a realistic dynamic scenario of measuring the acceleration response of a bridge due to the passage of a French Train $\grave{a}$ Grande Vitesse (TGV) in a laboratory environment.

• Geomechanics and Engineering
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• v.28 no.4
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• pp.359-374
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• 2022

Geology conditions are crucial in decision-making during the planning and design phase of a tunnel project. Estimation of the geology conditions of road tunnels is subject to significant uncertainties. In this work, the effectiveness of a novel regression method in estimating geological or geotechnical parameters of road tunnel projects was explored. This method, called Gaussian process regression (GPR), formulates the learning of the regressor within a Bayesian framework. The GPR model was trained with data of old tunnel projects. To verify its feasibility, the GPR technique was applied to a road tunnel to predict the state of three geological/geomechanical parameters of Rock Mass Rating (RMR), Rock Structure Rating (RSR) and Q-value. Finally, in order to validate the GPR approach, the forecasted results were compared to the field-observed results. From this comparison, it was concluded that, the GPR is presented very good predictions. The R-squared values between the predicted results of the GPR vs. field-observed results for the RMR, RSR and Q-value were obtained equal to 0.8581, 0.8148 and 0.8788, respectively.