• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.3
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• pp.172-178
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• 1998

Freezing-resistant plants can survive subzero temperatures by withstanding extracellular ice formation. During cold acclimation, their leaves accumulate antifreeze proteins (AFPs) that are secreted into the apoplast and have the ability to modify the normal growth of ice crystals. Three barley, two wheat and two rye cultivars were grown under two different temperature regimes (20/16$^{\circ}C$ and 5/2$^{\circ}C$, day/night). Apoplastic proteins from winter cereals were separated by SDS-PAGE and detected with antisera to AFPs from winter rye. Apoplastic proteins accumulated to much higher levels in cold-acclimated (CA) leaves compared with nonacclimated (NA) ones in winter cereals. After cold acclimation, the protein concentration of apoplastic extracts increased significantly from 0.088 $mgmL^{-1}$ to 0.448 $mgmL^{-1}$, with about 5-fold increment. Also, the apoplastic protein content per gram leaf fresh weight in CA leaves ranged from 31 $\mu\textrm{g}$ $(gFW)^{-1}$ to 120 $\mu\textrm{g}$ $(gFW)^{-1}$ with an averaged value of 77 $\mu\textrm{g}$ $(gFW)^{-1}$, and coefficients of variation of 54.9%. The CA leaves in Musketeer (a Canadian winter rye cultivar) showed the greatest AFPs and antifreeze activity followed by 'Geurumil' (a Korean winter wheat cultivar), and 'Dongbori l' (Korean facultative barley cultivar). The proteins secreted into the wheat leaf apoplast at CA condition were more numerous than those observed in winter rye, where two $\beta$-1,3-glucanase-like proteins (GLPs), two chitinase-like proteins (CLPs) and two thaumatin-like proteins (TLPs) accumulated during cold acclimation. The proteins in barley leaf apoplast at CA conditions were a little different from those in wheat leaves. The AFPs were various among and within species. More freezing-resistant cultivars had more clear and numerous bands than less freezing-resistant ones. The high determination coefficient ($R^2$ =91 %) between freezing resistance and AFPs per gram leaf fresh weight indicated that the amount of AFPs was highly related to freezing resistance in winter cereal crops.

• Geotechnical Engineering
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• v.5 no.4
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• pp.5-16
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• 1989

This paper statistically analyses the freezing soil by frost group and frost depth in Korea with data from soil testing in the Korea National Housing Corporation, the climate data provided by the Central Weather Office and the data on the frost depth from the National Construction Laboratory Institute. In this paper, freezing variable are analysed such as percentage finer than 0.02 m by weight, plasticity index, freezing index, water contents of soil and frost depth etc‥‥ The result of the analysis is as follows. 1) The frost depth of Korea depends on the properties of soil rather thank the characte fistic of area. 2) The distribution map of design freezing index in 57 cities is drawn up with the maxi- mum freezing index, during past 14 years, calculated by the average of the air temperature observed four times(03 : 00.09 00, 15 : 00, 21 : 00) a day. 3) By correcting the OLS line estimated from the relationship between freezing index and frost depth, a method of utlizing the line with the upper confidence limit of 99.9% int-distribution as predicted maximum frost depth is newly introduced.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.31 no.3D
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• pp.433-439
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• 2011

The frost depth(frost penetration) is used to install anti-frost heave layers in pavement designs. The freezing index is calculated by an annual accumulated value of multiplying the period of time with temperatures below zero, and the corresponding temperature. Therefore, the DAAT(daily average air temperature) calculation method may play an effect on the FI(freezing index). The Weather Observatory used to supply 4 average air temperatures per day, but currently supplies 8 per day. With this study, we divided the southern part(below FI=$350^{\circ}C{\cdot}day$) of the Korean peninsula into 6 areas according to site conditions(low embankment, embankment-cutting slope, and the cutting slope) and established a field measurement system for 15 positions to check the effects on the result of FI according to differing DAAT calculation methods. The air temperatures obtained by the field measurement system was used to calculate and compare the FI. As a result, the freezing index calculated based on the $DAAT_4(T_4)$ is normally greater by 3% than the one on $DAAT_8(T_8)$. In addition, the calibration equation for the freezing index using air temperatures was proposed through the research.

• Korean Journal of Agricultural and Forest Meteorology
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• v.11 no.4
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• pp.206-212
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• 2009

We investigated the patterns of freeze injury in dormant 'Changhowon Hwangdo' peach fruit by observing the extent of browning and germination of the branches that were treated with freezing temperature sets simulating the process of natural freezing incidences in orchards. Under the treatment of freezing temperature of $-15^{\circ}C$, the browning ratios were 15% for flower bud and less than 3% for both leaf bud and cambium. Under the $-20^{\circ}C$ treatment, the browning ratios were 40% for both flower and leaf buds and 1% for cambium. The browning ratios were 86%, 68% and 40% respectively for flower bud, leaf bud, and cambium under the $-25^{\circ}C$ treatment. All the samples showed 100% browning ratio under the $-30^{\circ}C$ treatment. The budburst ratios of leaf buds were 85%, 66%, 32%, and 0% under the -15, -20, -25 and $-30^{\circ}C$ treatments, respectively. The branches of peach fruit treated with the same freezing temperature showed different responses depending on the sampling date. In January the browning ratio was low and the budburst ratio was high whereas in February the opposite was the case, showing vulnerability of peach trees to low temperature after endo-dormancy release.

• Magazine of the Korean Society of Agricultural Engineers
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• v.31 no.2
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• pp.43-53
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• 1989

In this research programs, a series test was conducted to show the effects of freeze/thaw process on the various soil properties. The tests were carried out taken from the west sea shore of Korean peninsular and the west sea shore of Scotland, and their results are as follows; 1. There was a positive total heave in a freezing run, although water may he expelled for the sample initially. The water flow must he reverse' from expulsion to intake. 2. The confining pressure had an overriding influence on the heave and frost penetration, a sudden change of the axial strain at failure with strain rate was observed occuring at a strain rate between 10-5 and 10-6, and the initial friction angle of frozen clay was appeared zero. 3. There was shown a significant decrease in liquid limit of soil which was subjected to freeze/thaw process for the initial value of about 20% because of soil particles aggregation. 4. The cyclic freeze/thaw caused a sinificant reduction in shear strength and its thixotropic regain. The frozen/thawed soil exibited negative strength regain, particularly at high freeze/thaw cycles. 5. The freezing temperature greatly influenced on the failure strength of soils and this. Trend was more pronounced the lower the freezing temperature and shown the ductile failure with indistinct peaks.

• International Journal of Highway Engineering
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• v.15 no.3
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• pp.53-63
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• 2013

PURPOSES : This study is to evaluate moisture susceptibility of asphalt mixtures by using non-destructive impact wave and to determine durability so as to decrease the gap between before and after freezing in the future. METHODS : Using non-destructive impact wave, this study is to determine the dynamic modulus of asphalt specimen. Furthermore, the results obtained from two experiment accelerometers are used for the dynamic modulus determination. The dynamic moduli of specimens are compared with those of the freezing-thawing specimens. RESULTS : Test results showed that the dynamic modulus before freezing and thawing environment loads at each temperature dropped about 3.7% after the environmental loads. Furthermore, correlation analysis indicates that transition of dynamic modulus at each point is about 89.59%. CONCLUSIONS: Evaluation of asphalt mixtures using non-destructive impact wave has excellent repeatability and simple equipment for the test. Consequently, the method in the study will be useful for evaluating the characteristics of a various asphalt mixtures.

• Solar Energy
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• v.18 no.3
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• pp.95-105
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• 1998

In this study, basic design data which were required for development of highly efficient ice storage system with low temperature latent heat were experimentally obtained. The ice storage system considered in this study was the one that has been widly used in the developed country and called the ice-on-coil type. Using the system, the ice storage performance for various design parameters which were the flow direction and the inlet temperature of the secondary fluid was tested. In addition, the timewise variation of the interface profiles between the solid and the liquid were visualized, and the heat transfer characteristics of the Phase Change Material(PCM) in the ice storage tank were Investigated. During the freezing processes in the ice storage tank with several vertical tubes, decrease of the heat transfer area and the heat resistance of the ice layer made the increasing rate of ice packing factor(IPF) less. The total freezing energy for the upward flow of the secondary fluid was higher than that for the downward flow. The average ice storage efficiency for the upward flow of the secondary fluid was higher than that for the downward flow.

• Journal of the Korean Geosynthetics Society
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• v.13 no.4
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• pp.1-10
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• 2014

This paper presents the results of a field pilot test about deformation of water supply pipelines due to freezing temperature. There is a difference between for frost heaving load to act on the water supply pipelines. If the Marston-Spangler theory is only considered for the frost heaving load to act on the water supply pipeline, it is likely to deviate from the safety of the water supply pipeline, strains of the water supply pipeline show a tendency of smaller value than the value of numerical analysis.

• Food Science and Preservation
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• v.23 no.3
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• pp.438-444
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• 2016

This study was performed to determine the optimal freezing point for the reliable cold storage of Korean agricultural products, and to provide basic data for determining the storage temperature based on the quality characteristics. Additional supercooling temperature analysis was conducted to explore the possibility of supercooling storage. To determine the effects of quality characteristics on the freezing point, the hardness, acidity, moisture and sugar content were analyzed. The crops were frozen using customized cooling unit and their freezing and supercooling points were determined based on their heat release points. The freezing temperatures of garlic, leek, cucumber, hot pepper, grape, oriental melon, netted melon, peach, cherry tomato, plum, daikon, sweet persimmon, apple, sweet potato, mandarin, pear, and strawberry were -1.6, -0.5, -0.5, -0.7, -1.6, -1.6, -1.3, -0.8, -0.3, -1.1, -0.3, -1.7, -1.5, -1.5, -0.8, -1.5, and -$0.9^{\circ}C$, respectively; otherwise, supercooling points were -7.8, -3.7, -3.3, -4.9, -5.7, -4.6, -2.8, -3.3, -5.9, -4.2, -0.8, -4.7, -3.2, -3.7, -4.7, -4.2, and -$3.4^{\circ}C$, respectively. These results suggest that the ideal freezing temperature of crops could be estimated through freezing point depression because of their sugar content, and this technique should be used to maintain an optimum storage temperature. However, cold storage is complicated and further study is required because of the effects of long-term cold storage on the crops.

• Proceedings of the Korea Concrete Institute Conference
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• 1999.10a
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• pp.795-798
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• 1999

Deterioration induced by the freezing and thawing in concrete often leads to the reduction in concrete durability by the cracking or surface spalling. In this paper, the deterioration prediction model for concrete structures subjected to the irregular freeze-thaw was proposed from the results of accelerated laboratory test using the constant temperature condition and acceleration factor from the in-situ weather data.