• Title/Summary/Keyword: Freezing of water

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Experimental Study on the Freezing Conditions in a Ice-making Tube Using Flowing Subcooled Water (유동과 냉각수 이용 제빙관의 동결조건에 5관한 실험적 연구)

  • 박영하;현명택;이동원
    • Journal of Energy Engineering
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    • v.9 no.3
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    • pp.178-183
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    • 2000
  • 본 연구에서는 유동과 냉각수를 이용하는 아이슬러리 제조관정을 관찰하고, 이 시스템의 가장 큰 문제점인 관내동결 특성을 파악하였다. 시험수로는 수도수(city water)를 이용하였다. 시스템을 순환하는 시험수가 제빙관내에서 $0^{\circ}C$ 이하의 과냉각 조건이 형성되어 제빙관을 빠져 나오면서 축냉조에 설치된 충격판상에서 과냉각 해소가 일어났다. 이때 유동과냉각수는 내부에너지를 방출하면서 그 일부가 얼음 입자로 상변화되었고, 나머지는 실험장치를 계속순환하였다. 제빙관에서 시험수와 브라인의 열교환 방식을 대향류와 평행류 두 조건에서 시험수의 과냉각도는 Reynolds 수가 증가함에 따라 감소하는 경향을 보였고, 이들 결과 값으로부터 각각의 관계식을 유도하였다. 또한 브라인의 냉각속도가 0.29~0.53[$^{\circ}C$/min] 범위에서 시험수의 과냉각도는 브라인 냉각속도에 종속되지 않았다.

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Studies on the Cryopreservation and Survival after Thawing of Hamster Embryos (Hamster 수정란(受精卵)의 동결보존(凍結保存) 및 생존성(生存性)에 관한 연구(硏究))

  • Oh, Won Jin;Lee, Kyu Seung;Kim, Young Mook
    • Korean Journal of Agricultural Science
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    • v.13 no.1
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    • pp.82-89
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    • 1986
  • This experiment was carried out to determine the optimum freezing and thawing rates of the hamster embryos. The female hamsters were induced to superovulate by intraperitoneal injections of 30 i.u. PMSG and mated with males of the same strain of 4 days the PMSG injection. They were killed and embryos were flushed from the oviduct and uterine horn on 3 days after mating. Embryos were flushed with a modified Dulbecco's phosphate-buffered saline and equilibrated with 1.5 M-dimethylsulphoxide by a 3-step procedure. The freezing rates of the samples were $1^{\circ}C/min$ from room temperature to $-6^{\circ}C$ and the samples were seeded at $-6^{\circ}C$. After being held for 3 min at the seeding temperature, the rates were $0.3^{\circ}C/min$ from $-6^{\circ}C$ to $-35^{\circ}C$. From $-35^{\circ}C$ to $-70^{\circ}C$, the rates were divided into $0.1^{\circ}C/min$, $1^{\circ}C/min$ and $10^{\circ}C/min$, respectively. At $-70^{\circ}C$ the samples were plunged directly into liquid nitrogen. The samples were thawed at $4^{\circ}C/min$ and $12^{\circ}C/min$ from $-196^{\circ}C$ to $37^{\circ}C$, and for 2 min in $37^{\circ}C$ water bath, respectively. The average numbers of ovulation points and embryos recovered were 35.1 and 27.0 appearing 77.0% recovery rates. Eight cell embryos in the embryos recovered were 24.8. The survival rates of embryos according to the freezing rates were 55.5~67.7% at $0.1^{\circ}C/min$, 58.8~64.9% at $1^{\circ}C/min$ and 40.5~44.7% at $10^{\circ}C/min$, respectively. The survival rates at $10^{\circ}C/min$ were significantly low. The survival rates of embryos according to the thawing rates were 53.5% at $4^{\circ}C/min$, 53.7% at $12^{\circ}C/min$ and 59.1% in $37^{\circ}C$ water bath. The survival rates, in $37^{\circ}C$ water bath were slightly higher, but we did not find any differences among them. In conclusion, the best freezing rates of hamster embryos were $1^{\circ}C/min$ from the room temperature to $-6^{\circ}C/min$, $0.3^{\circ}C/min$ from $-6^{\circ}C/min$ to $-35^{\circ}C$ and $-0.1^{\circ}C/min$ or $1^{\circ}C/min$ from $-35^{\circ}C$ to $-70^{\circ}C$. The hamster embryos thawed for 2 min in $37^{\circ}C$ water bath showed the best survival rates.

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A comparative experimental study on the mechanical properties of cast-in-place and precast concrete-frozen soil interfaces

  • Guo Zheng;Ke Xue;Jian Hu;Mingli Zhang;Desheng Li;Ping Yang;Jun Xie
    • Geomechanics and Engineering
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    • v.36 no.2
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    • pp.145-156
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    • 2024
  • The mechanical properties of the concrete-frozen soil interface play a significant role in the stability and service performance of construction projects in cold regions. Current research mainly focuses on the precast concrete-frozen soil interface, with limited consideration for the more realistic cast-in-place concrete-frozen soil interface. The two construction methods result in completely different contact surface morphologies and exhibit significant differences in mechanical properties. Therefore, this study selects silty clay as the research object and conducts direct shear tests on the concrete-frozen soil interface under conditions of initial water content ranging from 12% to 24%, normal stress from 50 kPa to 300 kPa, and freezing temperature of -3℃. The results indicate that (1) both interface shear stress-displacement curves can be divided into three stages: rapid growth of shear stress, softening of shear stress after peak, and residual stability; (2) the peak strength of both interfaces increases initially and then decreases with an increase in water content, while residual strength is relatively less affected by water content; (3) peak strength and residual strength are linearly positively correlated with normal stress, and the strength of ice bonding is less affected by normal stress; (4) the mechanical properties of the cast-in-place concrete-frozen soil interface are significantly better than those of the precast concrete-frozen soil interface. However, when the water content is high, the former's mechanical performance deteriorates much more than the latter, leading to severe strength loss. Therefore, in practical engineering, cast-in-place concrete construction is preferred in cases of higher negative temperatures and lower water content, while precast concrete construction is considered in cases of lower negative temperatures and higher water content. This study provides reference for the construction of frozen soil-structure interface in cold regions and basic data support for improving the stability and service performance of cold region engineering.

A Study on Comparison of Phycocyanin Extraction Methods for Hyperspectral Remote Sensing of Cyanobacteria in Turbid Inland Waters (국내 담수역 남조류 원격탐사를 위한 피코시아닌 추출법 비교 연구)

  • Ha, Rim;Shin, Hyunjoo;Nam, Gibeom;Park, Sanghyun;Kang, Taegu;Song, Hyunoh;Lee, Hyuk
    • Journal of Korean Society on Water Environment
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    • v.32 no.6
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    • pp.520-527
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    • 2016
  • Phycocyanin (PC) is one of the water-soluble accessory pigments of cyanobacteria species, and its concentration is used to estimate the presence and relative abundance of cyanobacteria. In laboratory experiments, PC content of field data were determined using Sarada's freeze-thaw method in algal bloom season. The effectiveness of three selected extraction methods (repeated freeze-thaw method, homogenization, power control) for PC were determined. The extraction efficiency of phycocyanin was the highest (of the methods compared) when a single freezing-thawing cycle was followed by pre-sonication. Applying this optimized method to surface water of Korean inland waters, the average concentration distribution was estimated at $2.9{\sim}51.9mg/m^3$. It has been shown that the optimized pre-sonication method is suitable to measure cyanobacteria PC content for the characterization of inland waters. The approach and results of this study indicates the potential of effective methods for remote monitoring and management of water quality in turbid inland waters using hyperspectral remote sensing.

Pond System for Further Polishing of Constructed Wetland Effluent during Winter Season (연못을 이용한 동절기 인공습지 오수처리수의 추가 처리)

  • Yoon, Chun-Gyeong;Jeon, Ji-Hong;Kim, Min-Hee;Ham, Jong-Hwa
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.44 no.4
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    • pp.139-148
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    • 2002
  • Pilot study was performed to examine the feasibility of the pond system for further polishing of treatment wetland effluent from December 2000 to June 2001. The wetland system used for the experiment was highly effective to treat the sewage during the growing season, but it was less effective and its effluent was still high to discharge to the receiving water body. Therefore, the wetland effluent may need further treatment to prevent water quality degradation. Pond system could be used to hold and further polish the wetland effluent during the winter season and ots feasibility was evaluated in this study. Additional water quality improvement was apparent in the pond system during winter season, and the pond effluent could be good enough to meet the effluent water quality standards if it is properly managed. Timing of the pond effluent discharge appears to be critical for pond system management because it is a closed system and whole water quality constituents are affected by physical, chemical, and biological pond environments. Once algae started to grow in mid-April, constituents in the pond water column interact each other actively and its control becomes more complicated. Therefore, upper layer of the pond water column which is clearer than the lower layer my need be discharged in March right after ice cover melted. In the experiment, water quality of the upper water column was markedly clear in March than ant other times probably because of freezing-thawing effect. The remaining lower water column could be further treated by natural purification as temperature goes up or diluted with better quality of wetland effluent for appropriate water uses. This study demonstrated the feasibility of pond system for subsequent management of wetland effluent during the winter season, however, more study is needed for field application.

LNG-Vessels Hybrid Engine Seawater Desalination Complex System (LNG 선박 하이브리드 엔진 및 해수 담수화 복합 시스템)

  • Lim, Jae Jun;Lee, Dong-Heon;Byun, Gi-Sik;Kim, Gwan-Hyung
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2016.10a
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    • pp.663-664
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    • 2016
  • Temperature difference power generation using sea water is a method repeatedly closed liquefaction and gasification by using the ammonia (refrigerant) of the deep sea water and surface water with a temperature difference between turning the turbine. The larger the temperature difference between the nature of the temperature characteristic energy generation development, the better. This is the story that the surface waters of the deep-water temperature difference is large. But the winter is not large temperature difference between surface water and deep water has lowered energy efficiency. And desalination technologies accounted for 97% of the earth, but we can not eat the technology to convert sea water into fresh water, fresh water produced by the desalination technology that is available for various industries such as irrigation, drinking water in the vessel.In this paper, LNG transport vessels, based on the LNG transport ship to the temperature difference power generation using cold energy of thermal energy and LNG marine diesel engines, which use the existing order to improve the temperature of the surface waters of the season that is the current problem we propose that a complex development of desalination and desalination of seawater freezing research into hybrid research and utilizing the cold energy of the engine.

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Anti-stress Effect of Pyroligneous Liquid in SD Rats and ICR Mice

  • Kim, Mi Kang;Yu, Gu Yong;Tan-Lee, Blendyl Saguan;Oh, Hyun Jin;Dong, Kyung Woo;Jeong, Seung Hwa;Han, Seong Wook;Cheong, Jae Hoon
    • Biomolecules & Therapeutics
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    • v.11 no.4
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    • pp.249-256
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    • 2003
  • Pyroligneous liquid(PL) is produced by carbonizing Oak in 350-40$0^{\circ}C$. It is traditionally used for treating stress-related disorder, hepatic disease, immune disorder, G-I disorder and inflammatory disease. The aim of this study is to investigate anti-stress effects of PL. The experiments were performed with the use of young(9 weeks of age) male rats of SD strain and the male ICR mice (20-25 g). Animals of the normal group were not exposed to any stress and the control group were exposed to stress. The rats of the Ginseng, diazepam(BZ) and PL supplementary group were orally administered once a day 100 g of Ginseng extract-kg body weight, 5 mg of BZ/kg body weight and 1 ml of PL100 g body weight and then exposed to stress. The mice of the Ginseng, BZ and PL supplementary group were given water containing 100 g of Ginseng extract/100 ml potable water, 5 mg of BZ/kg 100 ml of drinking water and 10 ml of PL/100 ml of drinking water and exposed to stress. Animals were given materials for 7 days after stabilizing them, and then were given supplementary materials for 5 days with stress. They were stressed by immobilization for 30 minutes and then the animals were exposed to electroshocks for 5 minutes. We recorded stress-related behavioral changes of experimental animals by stressing them using the Etho-vision system and measured the levels of corticosterone in blood While stress suppressed locomotor activity of animals, PL-supplementation partially blocked the stress effect of locomotion in rats and mice, and also partially blocked stress-induced behavioral changes such as freezing, burrowing, smelling and rearing activity in rats and freezing, grooming, tailing and rearing in mice. The staying time of stressed rats and mice in open area decreased and in closed area it increased relatively in elevated plus maze test. However, these changes also partially were blocked by PL-supplementation. PL-supplementation decreased levels of blood corticosterone increased by stress in rats. These results suggest that PL protects partially the living organism from stress attack in some cases.

Durability Characteristics of Concrete Containing Lightly Burnt MgO Powder (저온 소성한 MgO 분말을 혼입한 콘크리트의 내구 특성)

  • Choi, Seul-Woo;Kim, Joo-Hyung;Lee, Kwang-Myong;Kwon, Yong-Gil;Jang, Bong-Seok
    • Journal of the Korea Concrete Institute
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    • v.23 no.5
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    • pp.609-615
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    • 2011
  • MgO concrete containing lightly burnt MgO powder at $850{\sim}1,000^{\circ}C$ may have a long-term expansibility characteristic. Such expansibility of MgO concrete can compensate the shrinkage at later ages since the hydration of the MgO is very slow. However, the addition of MgO delays the initial hydration of cement and increases the setting time of cement. Also, the porosity and pore-size distribution of the MgO concrete are different from OPC concrete. Therefore, in order to use MgO in practice, both mechanical and durability properties of MgO concrete should be carefully examined. In this study, durability tests on carbonation, freezing-thawing, and diffusion of chloride were carried out after 56 days of underwater curing at $20^{\circ}C$ to compare durability characteristics of 5% MgO-mixed concrete with those of OPC concrete. The results showed that MgO concrete shows a greater durability than the concrete with no MgO, because the micro structure in the MgO concrete is much denser due to its expansibility characteristic.

A Study of Heat Transfer during Freezing Process of Water in a Vertical Cylinder - Comparison of thermal storage performance on the working fluid direction - (수직원통형 축열조내 물의 응고과정시 열전달에 관한 실험적연구 - 작동유체의 유동방향에 따른 열저장성능 비교 -)

  • Heo, K.;Kim, Y.K.;Kim, Y.J.;Kim, J.K.;Yim, C.S.
    • Solar Energy
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    • v.15 no.2
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    • pp.77-90
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    • 1995
  • An effective heat transfer during freezing process was proposed in the vertical cylinder to improve the effectiveness of the heat storage. Vertical cylinder was filled with pure water in order to investigate ice-shape, temperature distribution of the liquid, temperature distribution of the cylinder tube wall, total heat storage per unit mass in the test section under the two experimental conditions; inlet temperature of working fluid is constant($-10^{\circ}C$) and inlet direction of working fluid is either upward flow or downward. Both the mean temperature of the liquid and temperature difference of cylinder tube wall in the upward were lower than those in the downward. In case that the initial temperature of water was $7^{\circ}C$ and $4^{\circ}C$, the shape of ice layer in the upward was more uniform than that in the downward. In case of $1^{\circ}C$, the shape of ice layer is formed by inlet direction of working fluid. Finally, time-varying total heat energy stored in the water in the upward was higher than that in the downward.

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Numerical Analysis of the Melting Process of Ice Using Plate Heaters with Constant Heat Flux (일정 열유속 조건의 판형 히터에 의한 해빙과정의 수치해석)

  • Kim, Hark-Koo;Jeong, Si-Young;Hur, Nahm-Keon;Lim, Tae-Won;Park, Yong-Sun
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.19 no.6
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    • pp.434-440
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    • 2007
  • One of the cold start problems of a FCV is the freezing of the water in the water tank when a FCV is not in operation and the surrounding temperature drops below $0^{\circ}C$. The ice in the tank should be melted as quickly as possible for a satisfactory operation of fuel cell vehicles. In this study, the melting process for the constant heat fluxes of the plate heaters was numerically calculated in the 2-D model of the tank and plate heaters. The enthalpy method and FVM code was used for this analysis. The changes of the temperature with heat fluxes and the heat transfer area could be investigated. The energy balance error was found to increase with the heat flux. From this numerical analysis, the proper heat flux value and some important design factors relating local overheating and pressurization of the water tank could be examined.