• Journal of the Korean Society for Precision Engineering
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• v.25 no.3
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• pp.165-175
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• 2008

An ankle-foot orthosis(AFO) is a brace for persons with gait disabilities to support or replace the function of ankle joint. Ankle-foot orthoses(AFO's) are usually prescribed to alleviate the drop-foot by constraining the excessive plantar flexion. The shape and the strength of the AFO are often based on 'trial and error' due to a lack of knowledge of the stress distribution in the AFO. In this study, an improved stress-freezing method was proposed to measure the stress distribution characteristics in the AFO. As a result, a photoelastic material with low freezing temperature was developed to measure the stresses under a person's direct contact loading condition. The three-dimensional stress-1rozen photoelastic models of AFO's for five stages of stance phase such as heel contact, foot flat, mid stance, heel off, and toe off were produced. The results of photoelastic analysis revealed that the stresses developed in the AFO were varied considerably from tensile to compressive or vice versa, during walking. At the posterior part of ankle joint in the AFO, the maximum compressive stress of 1.81MPa was observed in the mid stance, and the maximum tensile stress of 0.74MPa was observed during heel contact. The overall stress levels in the AFO's were low in the toe off phase. The results suggested that the posterior part of ankle joint might be the most fragile part in the AFO.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.2
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• pp.181-189
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• 2011

Cryopreservation protocols induce partially irreversible damage to mammalian sperm plasma membranes. Previous studies have indicated that adding cholesterol to the plasma membrane, as cholesterol-loaded-cyclodextrins, improves cryosurvival of sperm. Therefore, the purpose of this study was to determine if treating sperm of Markhoz bucks with cholesterol-loaded-cyclodextrins (CLC) (0, 0.75, 1.5, 2.25 and 3 mg/ml diluted $240{\times}10^6$ sperm/ml) in Tris-citric acid-glucose diluents with and without egg yolk (containing 5% glycerol) would improve the post-thaw sperm quality. The motion characteristics were evaluated with a Computer Assisted System Analyzer (CASA); acrosome integrity and vitality were measured with the triple-stain technique. Samples were recovered before and after freezing by means of putting straws into $37^{\circ}C$ water for 30 sec and then parameters were assessed. The results showed that the treatments significantly affected motility, progressive motility, recovery rate, curvilinear velocity, beat cross frequency, live sperm with reacted acrosome, live sperm with unreacted acrosome, dead sperm with reacted acrosorne, and dead sperm with unreacted acrosome during freezing (p<0.05). However; no significant differences were found for average path velocity, straight line velocity, amplitude of lateral head displacement, straightness and linearity (p>0.05). The best results were observed for extender containing 2.25 mg/ml ($240{\times}10^6$ sperm/ml) CLC supplemented with 2.6% egg yolk. In conclusion, the findings of this study indicate improved Markhoz sperm viability and motility following treatment in the presence of egg yolk.

• Journal of the Korean Geosynthetics Society
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• v.12 no.3
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• pp.23-29
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• 2013

The mechanical behavior of frozen soils is different from that of unfrozen soils due to the phase change between water and ice. The strength characteristics of frozen soils are governed by the intrinsic material properties such as grain size, ice and water content, air bubbles, and by externally imposed testing conditions such as temperature, freezing time, and strain rate. Especially, the strength of the frozen soils is generally higher than that of unfrozen soils due to ice binding capacity with soil particles, and is strongly affected by a highly complex interaction between the solid soil skeleton and the pore matrix, composed of ice and unfrozen water. In this study, the direct shear test and unconfined compression test are carried out inside of a large-scaled freezing chamber, and the relationships between cohesion and unconfined compression strength under various freezing temperature conditions are discussed.

• Food Science of Animal Resources
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• v.39 no.6
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• pp.1008-1014
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• 2019

This study investigated the effects of pressure-mediated protein changes on the ice nucleation temperature of pork loins. To variate chemical state of meat proteins, pork loin was pressurized at varying pressure levels (100-500 MPa) for 3 min, and moisture content, expressible moisture (EM) and differential scanning calorimetry (DSC) were analyzed. Although, all treatments showed similar moisture content, EM and degree of protein unfolding of pork loin showed different features as of 300 MPa. At moderate pressure treatments (100-200 MPa), all protein fractions were detected in DSC experiments, and pork loin had lower EM than control (p<0.05). Meanwhile, myosin and actin of pork loin treated at greater than 300 MPa were completely unfolded, and the treatments showed high EM compared to control (p<0.05). Unfolding of meat proteins was a factor suppressing ice nucleation, and the ice nucleation temperature tended to decrease with increasing applied pressure level. The ice nucleation characteristics of pressurized pork loin exhibited a potential application in freezing storage of pressurized meat with less tissue damage comparing to freeze fresh meat, and further exploration regarding the quality change after freezing of fresh and pressurized meat was warranted.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.1
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• pp.58-64
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• 2020

This present study was conducted to investigate protective effect of discontinuous Percoll gradient containing alpha-linolenic acid (ALA) before freezing process on viability, acrosome damage, mitochondrial activity, and oxidative stress of frozen-thawed boar spermatozoa. The separation of spermatozoa by discontinuous Percoll gradient was performed by different concentration of Percoll solution (45/90%) containing ALA combined with bovine serum albumin (BSA), and collected sperm in each Percoll layer was cryopreserved. To evaluate viability, acrosome damage, mitochondrial activity, and reactive oxygen species (ROS) level of frozen-thawed sperm, flow cytometry was used. Morphological abnormalities were observed under light microscope. In results, viability of sperm from 90% Percoll layer was higher than control and 45% Percoll group (p < 0.05). Separated sperm in 90% Percoll layer had lower acrosome damage and morphological abnormalities than control as well as viability, whereas 45% Percoll group was higher (p < 0.05). Similar with acrosome damage and abnormalities, mitochondrial activity was slightly enhanced and the population of live sperm with high ROS level was decreased by 90% Percoll separation, however, there was no significant difference. Supplementation of 3 ng/mL ALA into Percoll solution increased sperm viability and decreased population of live sperm with high ROS compared to control (p < 0.05). In conclusion, discontinuous Percoll gradient before freezing process could improve efficiency of cryopreservation of boar sperm through selection of sperm with high freezing resistance, and supplement of ALA during Percoll gradient might contribute suppression of ROS generation via stabilizing of plasma membrane during cryopreservation.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2022.11a
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• pp.175-176
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• 2022

By studying the characteristics of matrix insulated through heat generated through oxidation of iron powder, the basic research results on the possibility of buffering and applicability of Cold weather concrete as a curing method are presented. In order to prevent freezing due to a sharp decrease in temperature in the initial stage of curing, iron powder (Fe), powder activated carbon, which is a small amount of porous carbonaceous adsorbent, and salt (NaCl) as an oxidizing agent are replaced with iron powder admixture. As the curing temperature increases, the strength tends to increase, and when replacing the admixture at the same curing temperature, the strength slightly decreases. This is determined as a result of generating iron oxide through an oxidation reaction of iron powder, activated carbon, and NaCl generating a large amount of pores in the matrix. In addition, the internal temperature tends to increase as the mixing substitution rate increases, and it is judged that the oxidation heat of the iron powder mixture affects the increase of the internal temperature during curing. The higher the replacement rate of the iron powder mixture, the slightly lower the strength, but it is determined that freezing and melting that may occur in the early stage of curing can be prevented due to an increase in the initial internal temperature.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.41 no.6
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• pp.419-426
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• 2008

Physicochemical characteristics of gelatin extracted from abdominal skin of yellowfin tuna (Thunnus albacares), were investigated by comparing its proximate composition, pH, amino acid composition, viscoelastic properties, gel strength and SDS-PAGE patterns, with those of bovine and porcine gelatins. The effects of gelatin concentration, maturation time, heat and freeze treatments on the gel strength of yellowfin tuna abdominal skin gelatin were studied. Amounts of $\alpha$-chains, $\beta$- and $\gamma$-components of yellowfin tuna abdominal skin gelatin were higher than those of the two mammailan gelatins. Yellowfin tuna abdominal skin gelatin had the lowest imino acids (proline and hydroxyproline) content, which was consistent with that of other fishes. However, yellowfin tuna abdominal skin gelatin was highest in glycine, alanine, and lysine. The gel strengths of all gelatins were proportional to the concentration of gelatin, but yellowfin tuna abdominal skin gelatin exhibited the greatest gel strength at each concentration. Yellowfin tuna abdominal skin gelatin required a longer maturation time than the two mammalian gelatins to form a firm gel. Higher heating temperature decreased the gel strength of yellow fin tuna abdominal skin gelatin more than in the two mammalian gelatins. Freezing decreased the gel strength of bovine gelatin only slightly, but longer freezing times resulted in greater reductions in gel strength in the yellowfin tuna abdominal skin and porcine gelatins.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.29 no.12 s.243
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• pp.1291-1298
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• 2005

Since the interior shape of a pressure regulator is complex and the change of fluid resistance at each operation condition is rapid and big, the pressure regulator can become the major factor that causes big loss in pipelines. So the suitable pressure regulator modeling by each operation condition is important to obtain reliable results especially in small scale pipeline network analysis. And in order to prevent the condensation and freezing problems, it is needed to confirm both whether temperature recovery is achieved after passing by the pressure regulator's narrow neck and how much amount of low temperature area that can cause condensate accumulation is distributed by various PCV models at every inlet-outlet pressure ratio. In this research, the numerical model resembling P company pressure regulator that is used widely for high pressure range in commercial, is adopted as the base model of CFD analysis to investigate pressure regulator's flow characteristics at each pressure ratio. Additionally it is also introduced to examine pressure regulator's critical flow characteristics and possibility of condensation or freezing at each pressure ratio. Furthermore, the comparison between the results of CFD analysis and the results of analytic solution obtained by compressible fluid-dynamics theory is attempted to validate the results of CFD modeling in this study and to estimate the accuracy of theoretical approach at each pressure ratio too.

• Journal of Korean Society of Rural Planning
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• v.26 no.1
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• pp.89-99
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• 2020

This study was conducted as a basic research to create a sound and vital ecological environment in the city compared to the appearance characteristics of Gap stream(G-stream), which are the main stream of Banseok stream(B-stream), by analyzing the characteristics of waterbirds, including Wild Spot-billed Duck (S-Duck), that live in B-stream built as ecological stream. The waterbird survey was conducted by the line census for 3.2km, Jukdong-bridge to Jamiseon-bridge, from January to August 2018. The analysis of the survey was conducted with Relative species density(RD) of the emerging waterbird species, the Species diversity and Density Per Unit area for 100㎡(DPU). The waterbird survey results of B-stream was conducted 65 times. The five types of water birds that appeared during the survey were Spot-billed Duck(Anas poecilorhyncha), Teal(Anas crecca), Little Egret(Egretta garzetta), Great Egret(Egretta alba), and Grey Heron(Ardea cinerea). As a result, for S-Duck at B-stream, RD was 89.9%, monthly species diversity was simple as 0.3801 in January, 0.5943 in February and 0.3501 in August. The DPU of the S-Duck was 0.165/100㎡ in the B-Stream survey section which was 4.9 times higher than the main stream section, G-stream. The non-freezing zone of the city's small stream is expected to play an important role as a winter stop for wild birds such as S-Duck during the freezing period of the huge stream. For this reason, considering the ecological characteristics of wild waterbirds such as S-Duck when creating ecological stream, a: space and linear selection of waterways which can minimize the impact of natural enemy and increasing the number of walkers, b: management water-friendly plants in the low flow channel, c: arrangement walking-bicycle road will be necessary.

• Journal of the Korea Concrete Institute
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• v.15 no.2
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• pp.197-208
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• 2003

This paper is to determine the possibility of re-using waste concrete from Incheon city area. The strength test was conducted with five aggregate compounds which was replaced a natural aggregate with recycled aggregate. After checking the physical characteristics of recycled aggregate compounds, the mix design of recycled concrete was conducted. For the relatively comparison between natural and recycled compounds, while the unit aggregate weight was changed, other conditions were fixed. The freezing and thawing test which included fly-ash and super-plastezer were performed to check the durability and workability when recycling waste concrete. In the physical characteristics of recycled aggregate, it was found that the specific gravity of recycled coarse aggregate and recycled fine aggregate satisfied the first grade of recycle specification(KS), and all compounds of recycled aggregate also satisfied the second grade of absorption specification, Especially up to the 50% substitution of recycled aggregate is equal to or a bit lower than that of convention aggregate. In comparison with conventional concrete, the recycled concrete is lower than maximum by 7% in compressive strength decreasing rate after freezing-thawing test. From now, although most of recycled concrete was used to the building lot, subgrade, asphalt admixture, through the result. It was proved that possibility of re-using recycled aggregate as the substructure of bridge, retaining wall, tunnel lining and concrete structure which is not attacked the drying shrinkage severely.