• Journal of The Korean Society of Agricultural Engineers
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• v.47 no.6
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• pp.27-34
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• 2005

The purpose of this study is to improve the durability performance of polymer modified cement composites for repair of concrete under combined aging conditions. The experimental procedure was divided into three parts. First, the replacement level of mineral admixtures in polymer modified cement composites were determined in an experimental study based on a Box Behnken design. Second, the flow value, compressive strength and chloride permeability test of sixteen types of mixtures were conducted. Test results show that the polymer modified cement composites were effected on the improvement of the compressive strength and permeability performance. Third, the effects on the replacement level of silica fume mixture was evaluated by the compressive strength, chloride permeability, chemical resistance and repeated freezing and thawing cycles test. They demonstrated that the polymer modified cement composites using mixture of silica fume, fly ash, and blast furnace slag improved the durability performance.

• Archives of Pharmacal Research
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• v.19 no.6
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• pp.502-506
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• 1996

Protein X is one of the subunits of pyruvate dehydrogenase complex. The biological role of this protein has not been fully elucidated, mainly because of the difficulty in its dissociation from the tightly bound dihydrolipoamide acetyltransferase-protein X subcomplex. We have found that the detachment of protein X from acetyltransferase subunit can be easily accomplished by the cycles of freezing and thawing proces. Several lines of evidence including sodium dodecyl sulfate-polyacrylamide gel electrophoresis, N-terminal amino acid sequence analysis and acetylation with $[2^{14}C]$ pyruvate confirmed that the purified protein is protein X. The purified intact form of protein X was acetylated by $[2^{14}C]$ pyruvate in the presence of py-ruvate dehydrogenase subunit.The acetylation efficiency of this protein was lower than that of acetyltransferase and was not affected by the presence of acetyltransferase.

• Geomechanics and Engineering
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• v.25 no.1
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• pp.41-48
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• 2021

In cold regions, the integrity of the infrastructures built on weak soils can be extensively damaged by weathering actions due to the cyclic freezing and thawing. This damage can be mitigated by exploiting soil stabilization techniques. Generally, ordinary Portland cement (OPC) is the most commonly used binding material for investigating the chemo-hydromechanical behavior. However, due to the environmental issue of OPC producing a significant amount of carbon dioxide emission, calcium sulfoaluminate (CSA) cement can be used as one of the eco-sustainable alternatives. Although recently several studies have examined the strength development of CSA treated sand, no research has been concerned about CSA cement-stabilized sand affected by cyclic freeze and thaw. This study aims to conduct a comprehensive laboratory work to assess the effect of the cyclic freeze-thaw action on strength and durability of CSA cement-treated sand. For this purpose, unconfined compressive strength (UCS) and ultrasonic pulse velocity (UPV) tests were performed on the stabilized soil specimens cured for 7 and 14 days which are subjected to 0, 1, 3, 5, and 7 freeze-thaw cycles. The test results show that the strength and durability index of the samples decrease with the increase of the freeze-thaw cycles. The loss of the strength and durability considerably decreases for all soil samples subjected to the freeze-thaw cycles. Overall, the use of CSA as a stabilizer for sandy soils would be an eco-friendly option to achieve sufficient strength and durability against the freeze-thaw action in cold regions.

• International Journal of Highway Engineering
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• v.18 no.6
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• pp.123-130
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• 2016

OBJECTIVES : The objective of this research is to determine the moisture resistance of the freeze-thaw process occurring in low-noise porous pavement using either hydrated-lime or anti-freezing agent. Various additives were applied to low-noise porous asphalt, which is actively paved in South Korea, to overcome its disadvantages. Moreover, the optimum contents of hydrated-lime and anti-freezing agent and behavior properties of low-noise porous asphalt layer are determined using dynamic moduli via the freeze-thaw test. METHODS : The low-noise porous asphalt mixtures were made using gyratory compacters to investigate its properties with either hydrated-lime or anti-freezing agent. To determine the dynamic moduli of each mixture, impact resonance test was conducted. The applied standard for the freeze-thaw test of asphalt mixture is ASTM D 6857. The freeze-thaw and impact resonance tests were performed twice at each stage. The behavior properties were defined using finite element method, which was performed using the dynamic modulus data obtained from the freeze-thaw test and resonance frequencies obtained from non-destructive impact test. RESULTS : The results show that the coherence and strength of the low-noise porous asphalt mixture decreased continuously with the increase in the temperature of the mixture. The dynamic modulus of the normal low-noise porous asphalt mixture dramatically decreased after one cycle of freezing and thawing stages, which is more than that of other mixtures containing additives. The damage rate was higher when the freeze-thaw test was repeated. CONCLUSIONS : From the root mean squared error (RMSE) and mean percentage error (MPE) analyses, the addition rates of 1.5% hydrated-lime and 0.5% anti-freezing agent resulted in the strongest mixture having the highest moisture resistance compared to other specimens with each additive in 1 cycle freeze-thaw test. Moreover, the freeze-thaw resistance significantly improved when a hydrated-lime content of 0.5% was applied for the two cycles of the freeze-thaw test. Hence, the optimum contents of both hydrated-lime and anti-freezing agent are 0.5%.

• The Journal of Engineering Geology
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• v.24 no.2
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• pp.161-169
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• 2014

We present the results of an experimental physical weathering study that focuses on fresh and slightly weathered gneiss samples from the Wonju area of Korea. The study investigated changes in the physico-mechanical properties of these samples during accelerated laboratory-based weathering, including analyses of microfracture formation. The deteriorated samples used in the study were subjected to 100-150 freeze-thaw cycles, with index properties and microfracture geometries measured between each cycle. Each complete freeze-thaw cycle lasted 24 hours, and consisted of 2 hours of saturation in a vacuum chamber, 8 hours of freezing at $-21^{\circ}C{\pm}1^{\circ}C$, and 14 hours of thawing at room temperature. Specific gravity and seismic velocity values were negatively correlated with the number of freeze-thaw cycles, whereas absorption values tended to increase. The amount of deterioration of the rock samples was dependent on the degree of weathering of the rock prior to the start of the analysis. Absorption, specific gravity, and seismic velocity values can be used to infer the amount of physical weathering experienced by a gneiss in the study area. The sizes and density of microfracture in the rock specimens varied with the number of freeze-thaw cycles. We found that box fractal dimensions can be used to quantify the formation and propagation of microfracture in the samples. In addition, these box fractal dimensions can be used as a weathering index for the mid-and long-term prediction of rock weathering. The present results indicate that accelerated-weathering analysis can provide a detailed overview of the weathering characteristics of deteriorated rocks.

• Tunnel and Underground Space
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• v.21 no.2
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• pp.145-150
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• 2011

Variations of physical properties such as weight loss rate, wave velocity and uniaxial compressive strength after performing freeze-thaw cyclic test were measured in order to define weathering characteristics of sandstone and andesite. Weight change in specimens of the two rocks decreased with increasing the repetition number of freeze-thaw cyclic test. In particular, weight loss of andesite specimens was very irregular. P-wave velocity of sandstone specimens decreased more than 5%. On the other hand, P-wave velocity of andesite specimens do not vary up to 500 cycles and decreased more than 5% after 1000 cycles. This implies that the sandstone are easily weakened and loosened by weathering processes, while the andesite are relatively strong. In addition, the wave velocity changes of the andesite specimens coincident with the weight change. Uniaxial compressive strengths of the sandstone specimens slightly decreased at the early stage of the freezing-thawing cyclic test, then tended to be irregular after 64 cycles. In conclusion, the rock specimens showed smaller weight loss, less had lower strength reduction rate.

• Journal of Embryo Transfer
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• v.32 no.1
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• pp.1-8
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• 2017

Cryopreservation of germ cells from genetically proven animals could be a source of restoration tools from the risk of extinction or disappearance of wanted characteristics. Using frozen semen, the genetic gains of Korean native cattle have been increased greatly for 70 years. The preservation of genetic resources as a form of frozen semen straw has limited availability due to the numbers. To circumvent this weakness of frozen semen, we tested two re-freezing methods with different initial thawing temperatures using frozen Korean proven semen and rare breed semen from albino, black and chikso breeders. It has been known that human sperm could resist to cryo-damages by repeated freeze-thaw cycles, but not for Korean proven bulls number (KPN) or for rare breeds. Total 7 frozen semem from brindled(2), black(1), Korean Albino(2) and KPN(1) bulls were used for our research. After thawing straws under $5^{\circ}C/2min$ or $37^{\circ}C/40sec$ with low temperature water bath and thermo jug, spermatozoa were re-diluted with triladyl diluents after first thawing and re-frozen. Sperm motilities were compared between animals and treated groups after re-thawing. Mean values of motility and viability of refrozen/thawed sperm for expansion of the number of straws were significantly higher in $5^{\circ}C$ than in $37^{\circ}C$ (P < 0.05). However, the activity of viable sperm thawed at $5^{\circ}C$ was significantly decreased before refreezing. It is estimated that re-freezing of frozen semen from rare Korean native cattle is possible with resistant properties of survived spermatozoa.

• Journal of The Korean Society of Agricultural Engineers
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• v.52 no.1
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• pp.69-77
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• 2010

Research on permeable pavement like asphalt and concrete pavement with porous structure has been increasing due to environmental and functional need such as reduction of run off and flood, and increase and purification of underwater resource. This study was performed to evaluate permeability, strengths and durability of permeable polymer concrete (PPC) using recycled aggregate that is obtained from waste concrete. Also, 6mm length of polypropylene fiber was used to increase toughness and interlocking between aggregate and aggregate surrounded by binder. In the test results, regardless of kinds of aggregates and fiber contents, the compressive strength and permeability coefficient of all types of PPC showed the higher than the criterion of porous concrete that is used in permeable pavement in Korea. Also, strengths of PPC with increase polypropylene fiber volume fraction showed slightly increased tendency due to increase binder with increase of fiber volume fraction. The weight reduction ratios for PPC after 300 cycles of freezing and thawing were in the range of 1.6~3.8 % and 2.2~5.6 %, respectively. The weight change ratio was very low regardless of the fiber volume fraction and aggregates. The weight reduction ratios of PPC with fiber and aggregate were in the range of 1.3~2.7 % and 2.2~3.2 % after 13 weeks and was very low regardless of the fiber volume fraction and aggregates.

• Journal of Pharmaceutical Investigation
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• v.37 no.5
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• pp.281-286
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• 2007

For transdermal delivery of porcine placenta extract (PPE), various emulsion formulations were prepared and evaluated. Polysorbate surfactants were used as emulsifiers and various C-18 unsaturated fatty acids as enhancers. The skin permeation of PPE was tested using a cellulose nitrate membrane-loaded Franz cell apparatus. Among emulsifiers, Tween 20 provided higher penetration effect than did Tween 80. Meanwhile, of various fatty acids, linolenic acid (18:3) revealed the highest skin permeation of PPE than the other C-18 unsaturated fatty acids. Stability of PPE emulsions was determined by cycles of freezing and thawing processes. The stability of emulsions depended on the percentage of Tween 20. Minimum 20% of Tween 20 was required to stabilize emulsions at room temperature for several days. Taken together, our results suggest that Tween 20 and linolenic acids might be key components to formulate PPE emulsion to provide the desirable skin permeability and stability.

• Corrosion Science and Technology
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• v.3 no.3
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• pp.87-97
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• 2004

This paper presents a new approach with meso scale structure models to express mechanical property, such as stress - strain relationships, of concrete. This approach is successful to represent both uniaxial tension and uniaxial compression stress - strain relationship, which is in macro scale. The meso scale approach is also applied to predict degraded mechanical properties of frost-damaged concrete. The degradation of mechanical properties with frost-damaged concrete was carefully observed. Strength and stiffness in both tension and compression decrease with freezing and thawing cycles (FTC), while stress-free crack opening in tension softening increases. First attempt shows that the numerical simulation can express the experimentally observed degradation by introducing changes in the meso scale structure in concrete, which are assumed based on observed damages in the concrete subjected to FTC. At the end applicability of the meso scale approach to prediction of the degradation by combined effects of salt attack and FTC is discussed. It is shown that clarification of effects of frost damage in concrete on corrosion progress and on crack development in the damaged cover concrete due to corrosion is one of the issues for which the meso scale approach is useful.