• Asian-Australasian Journal of Animal Sciences
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• v.19 no.4
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• pp.486-494
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• 2006

In order to protect the spermatozoa against cold shock, hen egg yolk is widely used as a cryoprotective agent in semen freezing extenders for domestic animals. The protective action of yolk is largely presumed to be due to low density lipoproteins (LDL). The effects of LDL on sperm quality of bull and northern pike (Esox lucius) after freezing-thawing have been reported, but no study has been made to evaluate the effect of LDL on boar sperm motility and other characteristics. The experiment was carried out to investigate the effect of LDL on the freezing of boar sperm in 0.25 ml straws. The aim was to evaluate the quality of boar spermatozoa cryopreserved in the presence of LDL. Motility of semen cryopreserved in LDL was analyzed and compared to semen cryopreserved with Tris-citric acid-glucose (TCG) and Tris-citric acid-fructose (TCF), two basic freezing extenders containing egg yolk. Similarly, acrosome and plasma membrane integrity were also evaluated and compared to semen cryopreserved with TCG and TCF. Analysis of sperm quality after freeze-thaw showed that the motility, acrosome and plasma membrane integrity were improved with LDL in the extender, as compared to the TCG and TCF. The highest post-thaw integrity of acrosome and plasma membrane and motility were obtained with 9% LDL (w/v). Consequently, the optimum LDL concentration in the extender was 9%. It is also suggested that the concentration of LDL addition is important for the effect on boar sperm protection during freezing and thawing. The percentage of motile spermatozoa was significantly higher after freezing in 9% LDL than in TCG and TCF 54.4% versus 30.4% and 30.1% (p<0.05), respectively. The integrity of acrosome and plasma membrane were also significantly higher at 70.3% and 50.5% respectively with semen frozen in 9% LDL extender compared to TCG at 37.8% and 30.3% and TCF at 36.4% and 29.9%, respectively (p<0.05),. In conclusion, we propose that extender containing LDL extracted from hen egg yolk could be used as a cryoprotective media with a better efficiency than TCG and TCF. LDL improved boar semen quality, allowing better spermatozoa motility, acrosome and plasma membrane integrity after the freeze-thaw process. Furthermore, we found out that the extender with 9% LDL concentration significantly enhanced motility, acrosome and plasma membrane integrity of boar sperm after freezing and thawing.

• Journal of the Korea Concrete Institute
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• v.17 no.3 s.87
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• pp.385-392
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• 2005

The object of this study is to prove the quality and reliability of recycled aggregate concrete by finding a way to improve the durability of the material through the experiment on the accelerated carbonation, freezing and thaw, and drying shrinkage, analysing the influence on the durability to Promote more active use of recycled aggregate concrete. The result of study as follows. (1) Resistibility to the freeze and thaw of the recycled aggregate concrete showed relative dynamic modulus of elasticity over $90\%$ which is very good, and all cycles show $99.2{\~}91.0\%$ dynamic modulus of elasticity which is improved compared with the $97.5{\~}90.6\%$ relative dynamic modulus of elasticity of ordinary concrete made of broken stone. (2) Carbonated thickness of the recycled aggregate concrete and the normal concrete was similar or it appeared with the tendency which it diminishes more or less. (3) Length change rate in drying contraction of the recycled aggregate concrete made of the recycled aggregate was lower than the ordinary concrete made of the broken stone by $18.5{\~}3.9\%$ in all blending.

• Journal of the Korea institute for structural maintenance and inspection
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• v.20 no.1
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• pp.56-63
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• 2016

This experiment was compared and analyzed between the original surface paint through chloride penetration, neutralization, freeze-thaw and chemical corrosion resistance measuring internal structure and volume of voids in order to evaluate the effect of increase in durability of the newly modeled nano synthesized polymer paint painted on concrete surface which results improvement on air permeability to increase the durability of concrete structures. The test result of measuring volume of void and inner structure, concrete, spreaded with nano synthesized polymer paint, showed decreasing trend of pore volume in the range of less than $0.1{\mu}m$ and more than $0.3{\mu}m$. Also, using an electron microscope inside showed tightness of hydration texture. Chloride penetration depth of concrete, painted with nano synthesized polymer paint, was decreased more than 92% compared to non-painted concrete and 70% with water-based epoxy painted concrete. Especially, chemical corrosion resistance test set with aqueous solution of 5% sulfuric acid, non-painted concrete and water-based epoxy painted concrete showed weight loss of 4% after dipping for 12 days. On the other hand, concrete painted with nano synthesized polymer paint showed 1.7% weight loss under the same condition. Also, it showed great result of appearance under the criteria of Tsivilis et al.

• Asian-Australasian Journal of Animal Sciences
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• v.3 no.2
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• pp.91-96
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• 1990

This experiment was designed to determine whether ${\alpha}$-aminoisobutyric acid (AIB) can be used to predict membrane function of spermatozoa by measuring the uptake of AIB by fresh, stored and frozen-thawed rooster spermatozoa. When spermatozoa were stored at low temperature ($0{\sim}3^{\circ}C$) for 24 h. no difference was found in AIB uptake compared with fresh spermatozoa, whereas storage for 48 h resulted in a slight increase in AIB uptake by spermatozoa. On the one hand, the uptake of AIB by frozen-thawed spermatozoa was less than that by fresh spermatozoa. This suggests possibility of a different membrane transport system between spermatozoa preserved at low temperature ($0{\sim}3^{\circ}C$) and those frozen-thawed. Glycerol used as cryoprotectant may modify rooster sperm membrane in a different manner from cold preservation. Ouabaine ($10^{-4}M$) caused a slight decrease in AIB uptake, but caffeine ($10^{-2}M$) did not influence spermatozoal AIB uptake. These results indicate a successful application of AIB to rooster spermatozoa as a mean for measuring sperm membrane function and suggest a possible alteration of membrane transport system in rooster spermatozoa between cold ($0{\sim}3^{\circ}C$) and cryopreservation ($-196^{\circ}C$).

• Proceedings of the Korea Concrete Institute Conference
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• 1998.04a
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• pp.117-122
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• 1998

Concrete structures has been deteriorated by the freezing and thawing due to temperature gap. This study was conducted to evaluate durabilite of concrete which are increasingly demanded recently. Therefore the research of durability must be executed for application of waste foundry sand concrete real structures. Concrete durability must be executed for application of waste foundry sand concrete real structures. Concrete durability properties incorporating waste foundry sand was performed with the variable of W/C ratio, Sand/Waste foundry sand ratio and Air entrainment-Non air entrainment. Cylinder specimens were made and subjected to freezing and thawing cycle at $-18^{\cire}C$ and $4^{\cire}C$. Dynamic modulus of elasticity were evaluated as F/T cycle increase. The results show that strength of concrete is increased the W/C ratio decrease, the Sand/Waste foundry sand ratio increase when the concrete contains AE agent and decreasing WC ratio and AE concrete makes improved resistance of freezing and thawing improved. Especially, resistance of freezing and thawing is improved by Fine aggregate/Waste foundry sand ratio which is 50%, 25%, 0% in a row. Therefore it is turn out the waste foundry sand could be applied to concrete from the experiment.

• Proceedings of the Korea Concrete Institute Conference
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• 2004.11a
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• pp.537-540
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• 2004

Recently, for industrial development period, concrete structures in domestics have been increased. They were deteriorated by attack of carbonation, freeze-thaw and corrosion etc. In hence they were demolished and reconstructed, resulted in waste concrete particles. In this paper, waste concrete particles (WCP) by product from different crushing and selecting process were used in soil cement-based pavement in the various recycling. For using WCP in soil cement-based pavement, the Qualities, physical and chemical properties, of WCP should be researched. In the first step, the specified compressive strength of mortar for two types of clay sand soil and clay soil respectively was experimented to be 15 Mpa and then optimum mixing ratio of chemical solidification agent were decided in the range of $1.5\~3.0\%$ in the replacement with cement weight content. In the second step, based on the prior experimental results, recycling possibility of WCP in soil cement-based pavement was studied. In the result of experiment the mixing ratio of WCP were 5, 10, 15 and $20\%$ in the replacement with soil weight and the compressive strength of mortar was somewhat decreased according to the increase of the mixing ratio of WCP.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.4
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• pp.507-515
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• 2009

A series of experiments were conducted to determine the suitable freezing and thawing temperatures for the freezing of boar semen in 5 ml maxi-straws. The ultrastructure, in vitro fertilization (IVF) and artificial insemination (AI) of frozen-thawed semen were also be evaluated. The 5 cm freezing height gave the best results not only in post-thaw motility rate (54.00%), but also in normal acrosome morphology rate (NAR) (80.23%). There was no significant difference in the post-thaw motility between different thawing temperatures and corresponding thawing times (p>0.05); the group of $52^{\circ}C$ and 25 s gave the highest motility rate (45.00%). As a whole, not only from the motility but also the NAR, thawing at $42^{\circ}C$ was better than the other two treatments. In the freezing packages, 5 ml maxi-straw gave a little lower mobility (40%), viability rate (49.58%), plasma membrane integrity rate (53.91%) and NAR (52.65%) than the 0.25 ml straw, but there was no significant difference between the two straw volumes (p>0.05). The IVF capacity of frozen-thawed semen in this experiment was similar to fresh semen. From ultrastructure observation, the main damage to boar spermatozoa after freezing was seen in the acrosome, such as swelling and formation of vesicles. After AI in recipient Shanghai White sows, frozen-thawed semen from 5 ml maxi-straws and pellets produced 72.2% and 80% conception rate and 7.8 and 8 litter sizes, respectively, and there was no significant difference between the 5 ml maxi-straw and the pellet (p>0.05).

• Tunnel and Underground Space
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• v.22 no.1
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• pp.32-42
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• 2012

It is very difficult to capture the weathering characteristics of rock because of limitation caused by time and space. A new scheme of experiment that includes physical and chemical weathering processes was implemented on Cretaceous granitic rock samples from Kimhae area to investigate the variations of geomechanical properties of deteriorated rocks due to artificial weathering processes. The seismic velocity was found to decrease with increasing artificial weathering cycle. Effective porosity and absorption tend to increase with artificial weathering processes. The amount of deterioration of rock samples depend on pre-test degree of weathering. Effective porosity, absorption and seismic velocity can be used as the measure of weathering characteristics of granite in the study area. Weathering is accelerated by combined effect of physical and chemical weathering processes. The new experimental methodology conducted in this study has strong capability to analyze the weathering characteristics of rocks.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.13 no.3
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• pp.13-24
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• 1993

The influence of the bubble structure and strength characteristics on the freeze-thaw resistance of high strength concrete is investigated by the laboratory experiment. The test conditions are formed in the manner that water is continueusly supplied externally and the specimens were received severe weather actions from ordinary to significantly low temperatures. The experiments are performed in two stages. In the first stage, the relation between the durability to frost action and the bubble structure is analyzed especially with respect to the water-cement ratio and the amount of air. The AE and non-AE concrete specimens made of ordinary portland cement are used in the test. In the second stage, the non-AE concrete specimens using vibratory compaction to improve the durability to frost action, and the high watertight specimens of rapid hardening portland cement to increase their initial strength are produced and tested. The degree of watertightness of the specimens is determined by measuring the permeability of the specimens and the bubble structure of the high watertight concrete is also estimated.

• Journal of the Korea Institute of Building Construction
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• v.21 no.4
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• pp.281-291
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• 2021

To reduce the environmental load of the construction industry, there is a need to minimize construction and demolition by strengthening the eco-friendliness of building materials and extending the durable lifespan. Therefore, while many Hanok roof finishing methods have been proposed to address these problems, the current trend is to use the existing method due to issues such as economic feasibility, weight, and durability. The manufacturing method of clay roof tiles used as roofing materials for Hanok buildings is optimized by using a mixture of 64.5% Gyeongju clay, 15.0% kaolin, 15.0% FNS(Ferro Nickel Slag), and 5.5% MAS(Magnesia Aluminum Silicate) under optimal conditions. The results of the experiment involving firing at 1,125℃ showed that flexural strength of 12,102N, which is higher than the standard of KS F 3510, an absorption rate of 6.08%, a volume specific gravity of 2.15g/cm³, and the freeze-thaw properties were satisfied. A method for securing stable quality was studied.