• Title/Summary/Keyword: Free Fatty Acids

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Effect of the Extracting Condition on the Crude Fat and Free Fatty Acids of Red Ginseng Extract (추출조건이 홍삼엑기스의 지방과 유리지방당 조성에 미치는 영향)

  • 성형순;윤석권
    • Journal of Ginseng Research
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    • v.9 no.2
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    • pp.179-185
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    • 1985
  • The effect of temperature and ethanol concentration on lipids yield and free fatty acids were investigated during 1-5 times of 8 hours extraction. The crude lipids recovered was generally increased as the ethanol concentration increased while it was decreased at higher extraction temperature. Fatty acids composition in free fatty acids showed linoleum and plasmatic acids to be the major fatty acids, accounting 60% of 16 fatty acids identified. Fatty acids pattern was little affected by the changes in ethanol concentration while increase in temperature resulted significant changes in fatty acids composition.

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$H_2O_2$-Bis(2,4,6-trichlorophenyl) Oxalate Chemiluminescence Detection of Monodansyl Cadaverine Derivatives of Free Fatty Acids in High Performance Liquid Chromatography (Monodansyl Cadaverine유도체화된 유리지방산류의 $H_2O_2$-Bis(2,4,6-trichlorophenyl) Oxalate에 의한 고속액체크로마토그라피)

  • 이용문;문동철
    • YAKHAK HOEJI
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    • v.37 no.4
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    • pp.362-364
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    • 1993
  • The sensitive detection of free fatty acids was investigated by using H$_{2}$O$_{2}$-bis(2,4,6-trichlorophenyl) oxalate chemiluminescence system after monodansyl cadaverine labeling. Because dansvl moiety is well excited by this chemiluminescence system, monodansyl cadaverine was a prominent reagent to this system for the determination of free fatty acids. The cluent of 50mM tris-HCI buffer (pH 7.7)-acetonitrile (1:4, v/v) was run through TSK gel ODS 80 TM column. The reagent solutions were mixed with the eluent containing the monodansyl cadaverine derivative of fatty acids from the column. By this system, linolic acid was detected 50 fmol by injected amount.

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The Metabolites of a Marine Mollusk Mytilus edulis:- Isolation of Taurine and Compositions of Free Fatty Acids and Free Amino acids-

  • Cho, Yong-Jin;Son, Byeng-Wha;Choi, Hong-Dae
    • Natural Product Sciences
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    • v.1 no.1
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    • pp.5-9
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    • 1995
  • The metabolites of marine mollusk Mytilus edulis were isolated and characterized, revealing the presence of the rare free fatty acids and proteinogenic amino acids together with a non-proteinogenic free amino acid, taurine. The free fatty acids in this organism were particularly interesting for the presence of both unusual n-6 acid [20:4 (n-6)] and triple bond containing acid (2,5-octadecadiynoic acid). In addition to the proteinogenic amino acids commonly found in proteins, non-proteinogenic free amino acid taurine was isolated and the structure was determined by its physicochemical properties. Recently taurine has been given much interest in the molecular level because of diverse biological activities and the medicinal properties. Furthermore, the result of the analyses of analyses of free amino acids showed that glycine, glutamic acid, serine and alanine, which were considered to be related to the taste of this organism, are predominantly present.

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Studies on the Components in the Ethereal Extract of Panax ginseng(I) -Studies on the Free Fatty Acids- (인삼(人蔘)의 Ether추출물(抽出物)의 성분(成分)에 관한 연구(硏究)(I) -유리지방산(遊離脂肪酸)에 관한 연구(硏究)-)

  • Cook, Chae-Ho;An, Seung-Ho
    • Korean Journal of Pharmacognosy
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    • v.6 no.1
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    • pp.15-21
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    • 1975
  • The free fatty acids were prepared from the ethereal fraction of Panax ginseng. The prepared acids were methylated with diazomethzne. The methyl esters of saturated and unsaturated fatty acids were separated by the means of mercuric acetate method and column chromatography. The separated methyl esters were gaschromatographed and analyzed. The obtained conclusions were as follows. 1. The root of six-year old Korean Panax ginseng contains 0.28% of free fatty acids. 2. It was found that 24 kinds of free fatty acids existed in Panax ginseng. Among them, 22 kinds of free fatty acids were indentified by the gas chromatogram and the graphical method but the rest, 2 kinds of them were not identified by the only gas chromatographical data. The amount of each free fatty acid which was not identified was predominant and they were supposed to be unusual free fatty acids which would not commonly exist in nature. These results were shown in Table III. 3. $L_{EE}$ and $L_{EE}$ reported that n18:3 existed in Panax ginseng. However, in this experiment, n18:3 did not exist in Panax ginseng, and instead, peak XVI appeared between n18:2 and n18:3 as shown in Fig. 9.

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A Simple Colorimetric Method of Determining Free Fatty Acids in Serum (혈청내 유리지방산의 비색정량법)

  • Kim Sung Hoon;Park Hang Kyun;Mo Ki Choul;Huh Rhin Sou
    • Journal of the korean veterinary medical association
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    • v.19 no.1
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    • pp.54-61
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    • 1983
  • The present paper describes a simple colorimetric determination method of serum or plasma free fatty acids in various animal for those who are in the laboratories unequiped with high-cost newer analysing equipments and for those clinicians with low-cost a

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Thermal Acclimative Changes in the Different Lipid Fractions Composition of Fat Body of Eri-Silkworm, Philosamia Ricini (Ward.)

  • Singh, G.B.;Singh, M.K.
    • International Journal of Industrial Entomology
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    • v.4 no.1
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    • pp.13-17
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    • 2002
  • Present communication deals with quantitative determination of total lipid, triglycerides, total free fatty acids, phospholipids and total cholesterol in the fat body tissue of the silkworm adapted to low and high temperatures. At the end of spinning process is characterized by a marked cellular reorganization of the different lipid fraction of the fat body irrespective of thermal acclimation. Accordingly, the per cent composition of triglycerides of the total lipid is increased accompanied by a corresponding decrease in free fatty acids, phospholipids and cholesterol.

Esterification of Free Fatty Acids by Strong Acidic Ionic Liquids (강산성 이온성 액체에 의한 유리지방산의 에스테르화 연구)

  • Kim, Young-Joo;Lee, Jin-Suk;Kim, Deog-Keun;Rhee, Young-Woo;Han, Jeong-Sik
    • Korean Chemical Engineering Research
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    • v.45 no.3
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    • pp.286-290
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    • 2007
  • The esterification efficiency of several ionic liquids has been investigated to determine the feasibility for the conversion of free fatty acids to alkylester. Five ionic liquid catalysts having strong acidity, BPC[$AlCl_3$], BMIM[$Bf_4$], BMIM[$Pf_6$], EMIM[$Ntf_2$], BMIM[Otf], have been employed in this work. BPC[$AlCl_3$] has the highest esterification efficiency among the ionic liquid catalysts. Over 90% conversion efficiency has been achieved in the esterification of the simulated used cooking oil by BPC[$AlCl_3$] with two hours reaction time. Since BPC[$AlCl_3$] has several advantages such as high esterification activity, ease of separation from reaction mixture and reusability after treatment procedure, it will be a promising catalyst for the conversion of free fatty acids to esters in waste fats.

Influences of Free Fatty Acid on the Albumin Binding of Warfarin and Dansylsarcosine (Warfarin, Dansylsarcosine의 알부민결합에 미치는 유리지방산의 영향)

  • Bae, Jin-Woo
    • The Korean Journal of Pharmacology
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    • v.30 no.2
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    • pp.255-260
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    • 1994
  • There are some reports about the influences of free fatty acids on the albumin binding of drugs. But they were concerned to the limited free fatty acids, mostly of azapropazone-warfarin bidning site bound drugs and determination of dissociation and association constants by stopped flow technique. These data were not enough to make conculsions for the general tendency of free fatty acid to albumin binding. Therefore the influence of various saturated fatty acids of $C_{10{\sim}20}$, oleic acid and linoleic acid as unsaturated fatty acids to albumin binding of warfarin and dansylsarcosine were studied by equilibrium dialysis. The concentration of free drug was determined by spectrophotometer according to the molar ratios of 0, 0.5, 1, 2 and 4 between free fatty acid and albumin. There were significant increasing in the free durg concentration of warfarin and dansylsarcosine when the molar ratio for capric acid, lauric acid and palmitic acid was 4. The free warfarin concentration was increased significantly at a molar ratio of 4 between oleic acid and albumin. Therefore the albumin binding of durgs can be variated significantly by increased free fatty acid of diabetics and cause to the pharmacokinetic variation between healthy and diabetics.

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Changes in pasting properties and free fatty acids of different brown rice cultivar during storage

  • Choi, Induck;Kwak, Jieun;Yoon, Mi-Ra;Chun, Areum;Choi, Dong-Soo
    • Korean Journal of Food Preservation
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    • v.24 no.4
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    • pp.491-496
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    • 2017
  • Paddy rice is typically stored during postharvest until rice grain is processed into brown rice and milled rice by hulling and milling procedure, respectively. Recently, instead of storing paddy rice, storage of brown rice has been in the spotlight because it is more convenient and economically feasible. Different brown rice cultivars with varying amylose contents including waxy rice, medium-waxy rice, and non-glutinous rice were stored in room temperature storage for four months, and the changes in grain qualities of brown rice were evaluated. Amylose content significantly affected pasting properties in which rice cultivar with higher amylose content showed longer pasting time and higher peak viscosity. Storage also affected pasting viscosities, showing an increase in peak viscosity, but a decrease in breakdown viscosity. The changes in pasting viscosity during storage could be an important starch property for aged brown rice utilization. Waxy brown rice showed the weakest aging property in terms of free fatty acids (FFA) accumulation, whereas non-glutinous rice was more substantial grain quality against aging. The FFA values of two months storage were not significantly different from the initial FFA contents, suggesting that brown rice stored in room temperature for two months could be feasible for direct consumption of brown rice.

The Effects of Wolbiga-banha-tang on Obese Rats Fed a High-fat Diet (월비가반하탕이 고지방식이로 유도된 비만 쥐에 미치는 영향)

  • Na, Ga-young;Moon, Young-ho;Lee, Hye-in;Kim, Eun-ji
    • The Journal of Internal Korean Medicine
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    • v.37 no.6
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    • pp.1012-1021
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    • 2016
  • This study investigated the effects of Wolbiga-banha-tang (WBBT) on high fat diet-induced rats. We divided the rats into 4 groups: normal (fed a general-fat diet), control (fed a high-fat diet and no treatment), $WBBT{\times}1$ (fed a high-fat diet and 250 mg/kg of Wolbiga-banha-tang extracts), and $WBBT{\times}2$ (fed a high-fat diet and 500 mg/kg of Wolbiga-banha-tang extracts). We measured the body weight, total cholesterol, HDL cholesterol, LDL cholesterol, triglycerides, free fatty acids, total lipids, AST, ALT, and CBC in the serum of the obese rats for 7 weeks. There was a significant decrease in the control group, $WBBT{\times}1$, and $WBBT{\times}2$ in triglycerides and free fatty acids. The results suggest that WBBT is effective for lowering triglycerides and free fatty acids. Therefore, WBBT may be applied to the treatment of lipid metabolism in obesity and further clinical studies are needed.