• 제목/요약/키워드: Free Amino Acids

검색결과 1,670건 처리시간 0.034초

새우젓의 정미성분에 관한 연구 (THE TASTE COMPOUNDS FERMENTED ACETES CHINENSIS)

  • 정승용;이응호
    • 한국수산과학회지
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    • 제9권2호
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    • pp.79-110
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    • 1976
  • In Korea fermented fish and shellfish have traditionally been favored and consumed as seasonings or further processed for fish sauce. Three major items in production quantity among more than thirty kinds which are presently available in the market are fermented anchovy, oyster and small shrimp. They are usually used as a seasoning mixture of Kimchi in order to provide a distinctive flavor. Fermented small shrimp, Acetes chinensis is most widely and largely used ana occupies an important position in food industry of this country. But no study on its taste compounds has been reported. This study was attempted to establish the basic data for evaluating taste compounds of fermented small shrimp. The changes of such compounds during fermentation as free amino acids, nucleotides and their related compounds, TMAO, TMA, and betaine were analysed. In addition, change in microflora during the fermentation under the halophilic circumstance was also investigated. The samples were prepared with three different salt contents of 20, 30 and $40\%$ to obtain the proper degree of fermentation at a controlled tempeature of $20{\pm}2^{\circ}C$. The results are summarized as follows: Volatile basic nitrogen increased rapidly until 108 days of fermentation and afterwards it tended to increase slowly. Amino nitrogen also increased rapidly until 43 days of fermentation and then increased slowly. Extract nitrogen increased and marked the maximum value at 72 day fermentation and then decreased slowly. ADP, AMP and IMP tended to degrade rapidly while hypoxanthine increased remarkably at 27 day fermentation but slightly decreased at 72 day fermentation. It is presumed that the characteristic flavor of fermented small shrimp might be attributed to the relatively higher content of hypoxanthine. In the free amino acid composition of fresh small shrimp abundant amino acids were proline, arginine, alanine, glycine, lysine, glutamic acid, leucine, valine and threonine in order. Such amino acids like serine, methionine, isoleucine, phenylalanine, aspartic acid, tyrosine and histidine were poor. In small shrimp extract, proline, arginine, alanine, glycine, lysine and glutamic acid were dominant holding $18.5\%,\;14.6\%,\;10.8\%,\;8.7\%,\;8.1\%\;and\;7.7\%$ of total free amino acids respectively. The total free amino acid nitrogen in fresh small shrimp was $63.9\%$ of its extract nitrogen. The change of free amino acid composition in the extract of small shrimp during fermentation was not observed. Lysine, alanine glutamic acid, proline, glycine and leucine were abundant in both fresh sample and fermented products. The increase of total free amino acids during 72 day fermentation reached approximately more than 2 times as compared with that of fresh sample and then decreased slowly. Fermented small shrimp with $40\%$ of salt was too salty to be commercial quality as the results of organoleptic test showed. It is found that 72 day fermentation with $20\%\;and\;30\%$ of salt gave the most favorable flavor. It is convinced that the characteristic flavor of fermented small shrimp was also attributed to such amino acids as lysine, proline, alanine, glycine and serine known as sweet compounds, as glutamic acid with meaty taste, and as leucine known as bitter taste. The amount of betaine increased during fermentation and reached the maximum at 72 day fermentation and then decreased slowly TMA increased while TMAO decreased during fermentation. The amount of TMAO nitrogen in fermented small shrimp was $200mg\%$ on moisture and salt free base. Betaine and TMAO known as sweet compounds were abundant in fermented small shrimp. It is supposed that these compounds could also play a role as important taste compounds of fermented small shrimp. At the initial stage of fermentation, Achromobacter, Pseudomonas, Micrococcus denitrificans which belong to marine bacteria were isolated. After 40 day fermentation, they disappeared rapidly while Halabacterium, Pediococcus, Sarcian, Micrococcus morrhuae and the yeasts such as Saccharomyces sp. and Torulopsis sp. dominated. It is concluded that the most important taste compounds of fermented small shrimp were amino acids such as lysine, proline, alanine, glycine, serine, glutamic acid, and leucine, betaine, TMAO and hypoxanthine.

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자화지정(Viola mandshurica)의 아미노산과 유용성분 비교 분석 (Comparative Analysis of the Amino Acids and Effective Components from Viola mandshurica)

  • 이양숙;김남우
    • 한국식품저장유통학회지
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    • 제15권5호
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    • pp.699-705
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    • 2008
  • 본 연구는 한방생약재로 사용되고 있는 자화지정의 식품학적 가치와 식품소재로서의 개발 가능성을 검토하기 위하여 국내에서 채집된 자화지정의 기원식물인 제비꽃과 한방재료로 유통, 판매되고 있는 중국산 자화지정을 비교, 분석하였다. 분석 결과 자화지정 꽃의 환원당과 유리당이 각각 10,485.11 mg%와 118.85 mg%로 가장 많았으며, 수용성 단백질과 폴리페놀의 함량도 각각 1,259.26 mg%와 2366.67 mg%로 함량이 높은 것으로 확인되었다. 플라보노이드 함량은 중국산 잎에서 2,370.75 mg%이었으며, 잎은 1,844.43 mg%을 함유하였다. 유리아미노산은 잎(5,345.21 mg%) > 뿌리(4,692.24 mg%) > 꽃(3,807.87 mg%) > 중국산 잎 (3,613.81 mg%)의 순으로 특히 뿌리에는 arginine의 함량이 전체의 55 %이상 차지하였으며 모든 부위에서 valine, aspartic acid, glutamic acid, alanine, arginine 등의 함량이 비교적 높은 것으로 나타났다. 아미노산 유도체는 잎에서 9,058.80 mg%로 가장 많이 함유하였으며 $\alpha$-aminoadipic acid는 모든 부위에서 50% 이상을 함유하였으며 특히 잎에서는 아미노산 유도체(7,124.23 mg%) 중 약 80%를 차지하였다. 무기질 함량은 중국산 잎이 5,917.57 mg%로 가장 높았으며, 모든 부위에서 K, Ca와 Mg의 함량이 매우 높았으며, 특히 잎과 꽃의 95% 이상을 차지하였다. 이상의 결과 국내에서 채집된 자화지정의 잎이 중국산 잎보다는 환원당과 폴리페놀, 플라보노이드 그리고 무기질의 함량은 낮았으나 수용성 단백질과 아미노산을 다량 함유하였으며, 국내에서 채집된 자화지정의 뿌리와 꽃에서도 유용한 유용성분들을 다량 함유하므로 음료나 건강보조식품 등 다양한 기능성 제품 개발에 이용될 수 있는 한방약용자원인 것으로 사료된다.

한국산(韓國産) 개암 종실(種實)의 아미노산 조성(組成)에 관한 연구 (Studies on the Amino Acid Components of Korean Hazel Nut)

  • 김미란;고영수
    • 한국식품과학회지
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    • 제13권1호
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    • pp.1-5
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    • 1981
  • 한국산 개암종실의 유리 및 전(全)아미노산의 종류 및 함량을 아미노산 자동분석기 (Technicon PNC-1 type)에 의해서 정량한 결과 유리아미노산의 전체량은 4.14mg/g이고 전(全)아미노산의 총함량은 180.64 mg/g이며 유리 아미노산 중 아르기닌의 함량이 가장 많고 글루탐산, 프롤린, 알라닌 및 발린의 순이었고 전(全)아미노산의 경우 역시 아르기닌의 함량이 가장 높고 그 다음이 글루탐산, 프롤린, 로이신 및 아스파르트산으로서 개암중에는 영양학적으로 중요한 필수아미노산을 모두 함유하고 있어서 영양상 뿐만 아니라 풍미도 나쁘지 않다고 생각된다.

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Experimental Measurement and Correlation of two α-Amino Acids Solubility in Aqueous Salts Solutions from 298.15 to 323.15 K

  • Abualreish, Mustafa Jaipallah;Noubigh, Adel
    • Korean Chemical Engineering Research
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    • 제58권1호
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    • pp.98-105
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    • 2020
  • By the gravimetric method at atmospheric pressure, the solubility of two α-amino acids was resolved over temperatures from (293.15 to 323.15) K. The α-amino acids studied were L-arginine and L-histidine. Results showed a salting-out effect on the solubility of the tested amino compounds. It is obvious that there was an increase in the solubility, in aqueous chloride solutions, with the increasing temperature. Results were translated regarding the salt hydration shells and the ability of the solute to form hydrogen-bond with water. The solubility data was precisely associated with a semi-empirical equation. The standard molar Gibbs free energies of transfer of selected α-amino compounds (ΔtrGo) from pure water to aqueous solutions of the chloride salts have been calculated from the solubility data. The decrease in solubility is correlated to the positive (ΔtrGo) value which is most part of the enthalpic origin.

백미(白米)와 교맥분(蕎麥粉) 혼식(混食)의 영양가개선(營養價改善)에 대(對)한 연구(硏究)(2) - 백미(白米)와 교맥분(蕎麥粉)의 혼합사료(混合飼料)로 사육(飼育)한 흰쥐의 Growth Gain과 혈장(血漿) 및 간장중(肝臟中)의 유리(遊離)아미노산(酸) Level에 대(對)하여 - (Improving the Nutritional Value of Mixed Diet of Rice and Buckwheat(2) - Growth Gain and the Level of Free Amino Acids in Plasma and Liver of Rats Fed on Rice Diet Adding Buckwheat -)

  • 이현기;이숙희;전영수
    • 한국식품영양과학회지
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    • 제2권1호
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    • pp.23-30
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    • 1973
  • Wistar strain male rats were divided into four different diet groups and the control group was fed on 7-percent casein diet (C), the second with a mixture of 80% of rice and 20% of buckwheat (RW), the third with rice only (R), and the fourth with a mixture of rice and buckwheat of equal nitrogen level (RWS). Each diet group consisted of 6 rats was fed for three weeks by the ad libitum feeding method. The results of the experiment, i.e., the growth gain and change in the level of free amino acids in the plasma and liver determined by the micro-bioassay method were as follows. 1. The group C showed the largest growth gain and the rest did in the order of RW group, R group. and RWS group. 2. Rats fed on diet of high protein score showed high level of free amino acids tryptophan, lysine, and threonine in the plasma and liver except for the case of lysine in the liver. 3. Protein score would be estimated by the level of free amino acids in the liver and plasma.

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천연꿀과 사양꿀의 성분 분석 (Chemical Composition of Korean Natural Honeys and Sugar Fed Honeys)

  • 김세건;홍인표;우순옥;장혜리;장재선;한상미
    • 한국식품영양학회지
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    • 제30권1호
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    • pp.112-119
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    • 2017
  • In this study, we investigated and compared some chemical properties of Korean natural honeys and sugar-fed honeys for their quality characteristics. The natural honey samples were monofloral from chestnut and acacia flowers, and the sugar-fed honey samples were collected from honeybees feeding on sugar cane and sugar beet. The chemical properties of the honey samples, such as moisture, total protein, total lipids, ash, carbohydrate, minerals, vitamins, and free amino acids were determined. The moisture content was $18.5{\pm}0.9%$ in natural acacia honey, $17.2{\pm}0.9%$ in natural chestnut honey, $19.6{\pm}0.9%$ in sugar cane-fed honey, and $24.8{\pm}%$ in sugar beet-fed honey. The total protein and ash contents were the highest in natural chestnut honey. Maltose and sucrose were not detected in natural honeys but were detected at 2~7% in sugar-fed honeys. The vitamin, mineral, and free amino acids contents of natural honeys were higher than sugar-fed honeys. The natural chestnut honey is the highest in honeys. These results confirmed that the quality of natural honey was better than that of sugar-fed honey. Also, the vitamin, mineral, and free amino acids contents are potential characteristics for distinguishing between natural and sugar-fed honeys.

Chemical Characteristics of Commercial Korean Soy Sauce Produced by Alkali Treatment Following Acidic Hydrolysis

  • Cho, Woo-Jin;Kim, Hun;Jeong, Eun-Jeong;Lee, Young-Mi;Kim, Hyoun-Jin;Lee, Jung-Suck;Cha, Woung-Jun
    • Preventive Nutrition and Food Science
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    • 제7권4호
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    • pp.427-431
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    • 2002
  • Alkali treatment following acidic hydrolysis (ATAH) is a commonly used practice for reducing the levels of monochloropropanediol in commercial Korean soy sauce. This study investigated the chemical compounds produced in commercial Korean soy sauce made by ATAB. The levels of amino-N, total acidity, pH, salinity and Brix of the soy sauce were 6.66%, 2.52%, 19.81%, 4.57 and 35.01, respectively. The major fatty acids were C18:2n-6, C16:0, Cl8:ln-9 and C18:3n-6. The concentration of polyunsaturated fatty acids were especially high, with of C18:2n-6 (49.8%) being the highest followed by C18:3n-6 (3.8%) and C18:3n-3 (2.4%) in that order. Among the non-volatile organic acids, the concentration of levulinic acid (1,206.28 mg/100 g) was the highest, while the taste value of citric acid was the highest. Among the ATP related compounds, IMP concentration (31.19 mg/100 g) was highest followed by AMP, hypoxanthine and GMP in that order. The concentrations of free and total amino acids in soy sauce were 6,136.94 mg/100 g and 8,702.76 mg/100 g, respectively. On the other hand, the taste value of glutamic acid, a major amino acid flavor determinant in soy sauce, was highest of all the amino acids, which is desirable since most free amino acids such as methionine, histidine and phenylalanine have a bitter taste that detracts from the flavor of soy sauce.

인공재배 복령(茯笭)의 성분조성 (The Components of Cultivated Poria cocos)

  • 최옥법;조덕봉;김동필
    • 한국식품영양학회지
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    • 제9권4호
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    • pp.438-440
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    • 1996
  • 인공재배한 백복령의 성분을 분석한 결과 일반성분은 수분 58%, 회분 1.8%, 지방 0.9%, 단백질 0.6% 섬유소 0.3%로 나타났으며 재배장소와 방법, 수확시기, 건조조건에 따라 차이가 난다. 부기성분은 ICP로 측정한 결과 Zn 0.63mg%, Cu 0.18mg%, Fe 1.56mg%, Mn 1.62mg%, Mg 5.28mg%, Ca 12.59mg%, Na 10.12mg%, K 52.39mg%로 나타나 K, Ca, Na 등의 양이온 무기성분이 상당량 함유되어 있었다. 복령 물추출물의 유리당은 glucose만 검출되었고 함량은 1.16mg%였다. 아미노산은 구성아미노산 13종, 유리아미노산 9종이 확인되었고 함량은 각각 1247.17mg%, 174.44mg%였으며, Phe, Iso, Leu, Tyr, His 함량이 높게 나타났다.

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Rapid Enzymatic Fermentation of Anchovy Sauce by Protease

  • Jeong, Yong-Jin;Seo, Ji-Hyung
    • Preventive Nutrition and Food Science
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    • 제9권3호
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    • pp.213-217
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    • 2004
  • We evaluated the possibility of rapid fermentation of anchovy sauce using a commercial protease. The fermentation characteristics were monitored by response surface analysis. The content of total nitrogen was high (around 1 %) with fermentation at 51.7~57.5$^{\circ}C$ after 10.2~16.4 hours, but rapidly decreased at higher temperatures (6$0^{\circ}C$ or over), while the $R^2$ of polynomial equation was 0.9185 (p<0.05). The amino acid content rapidly decreased to approximately 600 mg% and less at high temperature (6$0^{\circ}C$ and over), and the $R^2$ was 0.9578 (p<0.01). The free amino acids were affected more by fermentation time when fermentation temperature was lower, and the $R^2$ for total free amino acids was 0.8496 (p<0.10). The $R^2$ for sweet free amino acids was 0.9144 (p<0.05). According to the results of this study, the optimal conditions for anchovy sauce fermentation were predicted to be 52.5~56.9$^{\circ}C$ and 13.3~16.4 hours, and the predicted values and actual values of each response variable were similar to each other when the fermentation was performed at a random point within the optimal range. Also, the comparison of the quality between the quick anchovy sauce and sauces currently on the market showed that the content of sweet amino acids was higher in the former than in the latter.