• Title/Summary/Keyword: Free Amino Acid Fatty Acid

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Effects of Dietary Garlic Extracts on Whole Body Amino Acid and Fatty Acid Composition, Muscle Free Amino Acid Profiles and Blood Plasma Changes in Juvenile Sterlet Sturgeon, Acipenser ruthenus

  • Lee, Dong-Hoon;Lim, Seong-Ryul;Ra, Chang-Six;Kim, Jeong-Dae
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.10
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    • pp.1419-1429
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    • 2012
  • A series of studies were carried out to investigate the supplemental effects of dietary garlic extracts (GE) on whole body amino acids, whole body and muscle free amino acids, fatty acid composition and blood plasma changes in 6 month old juvenile sterlet sturgeon (Acipenser ruthenus). In the first experiment, fish with an average body weight of 59.6 g were randomly allotted to each of 10 tanks (two groups of five replicates, 20 fish/tank) and fed diets with (0.5%) or without (control) GE respectively, at the level of 2% of fish body weight per day for 5 wks. Whole body amino acid composition between the GE and control groups were not different (p>0.05). Among free amino acids in muscle, L-glutamic acid, L-alanine, L-valine, L-leucine and L-phenylalanine were significantly (p<0.05) higher in GE than in control. However, total whole body free amino acids were significantly lower in GE than in control (p<0.05). GE group showed higher EPA (C22:6n3) and DHA (C22:5n3) in their whole body than the other group (p<0.05). In the second experiment, the effects of dietary garlic extracts on blood plasma changes were investigated using 6 month old juvenile sterlet sturgeon averaging 56.5 g. Fish were randomly allotted to each of 2 tanks (300 fish/tank) and fed diets with (0.5%) or without (control) GE respectively, at the rate of 2% of body weight per day for 23 d. At the end of the feeding trial, blood was taken from the tail vein (n = 5, per group) at 1, 12, and 24 h after feeding, respectively. Blood plasma glucose, insulin and the other serological characteristics were also measured to assess postprandial status of the fish. Plasma glucose concentrations (mg/dl) between two groups (GE vs control) were significantly (p< 0.05) different at 1 (50.8 vs 62.4) and 24 h (57.6 vs 73.6) after feeding, respectively, while no significant difference (p>0.05) were noticed at 12 h (74.6 vs 73.0). Plasma insulin concentrations (${\mu}IU$/ml) between the two groups were significantly (p<0.05) different at 1 (10.56 vs 5.06) and 24 h (32.56 vs 2.96) after feeding. The present results suggested that dietary garlic extracts could increase dietary glucose utilization through the insulin secretion, which result in improved fish body quality and feed utilization by juvenile sterlet sturgeon.

Quality Characteristics of Jeju Traditional Doenjang (제주 전통된장의 품질 특성)

  • Oh, Hyun-Jeong;Lim, Ja-Hun;Lee, Ju-Yeon;Jeon, Si-Bum;Kang, Hye-Young;Oh, You-Sung;Oh, Young-Ju;Lim, Sang-Bin
    • Culinary science and hospitality research
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    • v.15 no.2
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    • pp.298-308
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    • 2009
  • In order to establish the quality standard of Jeju traditional Doenjang(soybean paste), general components, free sugar, organic acid, fatty acids, amino acids, and isoflavones contents were analyzed. Moisture, crude ash, crude protein, crude lipid, acid value, pH, and salinity were 37.33~47.43%, 12.75~15.97%, 11.06~14.28%, 0.08~0.53%, 17.83~27.55 mg/g, 4.82~5.12, and 10.66~13.91% respectively. The content of amino type nitrogen was 301.06~573.89 mg%. The compositions of free sugar were glucose (0.81~10.22 mg/g), maltose(0.64~5.28 mg/g), sucrose(0.78~3.86 mg/g), and fructose(0.72~1.11 mg/g). The major organic acid was lactic acid (89.30~180.39 mg/g). Total free amino acids were from 13.89 to 15.51%, which makes no difference between the test samples. Among amino acids, glutamic acid was abundant (2.12~2.55%). The contents of isoflavones were 274.87~431.96 mg/kg, less than those of commercial soybean paste. In fatty acid composition of soybean paste, the most abundant fatty acid was found to be linoleic acid as 61.54~66.79% in Jeju traditional Doenjang. This results might be caused by the differences of brewed periods, microflora, processing methods and used soybean cultivars.

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Physicochemical Composition of Petasites japonicus S. et Z. Max. (머위 (Petasites japonicus S. et Z. Max.)의 이화학적 성분)

  • Cho, Bae-Sick;Lee, Jae-Joon;Ha, Jin-Ok;Lee, Myung-Yul
    • Food Science and Preservation
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    • v.13 no.5
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    • pp.661-667
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    • 2006
  • This study was carried out to analyze the major chemical component of the leaf and stem of dried Petasites japonicus S. et Z. Max.. Comparing proximate composition of leaf and stem of Petasites japonicus as dry matter basis, stem contained higher moisture, crude fat, crude ash and carbohydrate, with less crude protein. The main component of free sugar and disaccharide in both leaf and stem were fructose and sucrose, respectively. Total amino acids of leaf and stem were 6,853.32 mg% and 2,350.61 mg% respectively. Although the amino acid composition of leaf and stem were different glutamic acid and aspartic acid were the major amino acids in samples. The major fatty acids of total lipids were linolenic acid in leaf and linoleic acid in stem. The ratios of unsaturated fatty acids to saturated fatty acid were 3.93 in leaf and 3.44 in stem. The unsaturated fatty acid content of samples were 3 times higher than those of saturated fatty acid contents. Oxalic acid was the major organic acids in leaf and stem. The contents of vitamin A, C and E were higher in leaf than in stem. The mined compositions of both leaf and stem were composed in order of K, Mg, Ca, Fe, Na, and Zn.

Studies on the Nutritional Value of Loach Misgurnus mizolepis (미꾸라지의 영양성분에 대한 연구)

  • Kim, Hee-Sook;Lee, Hyun-Ki
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.14 no.3
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    • pp.296-300
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    • 1985
  • This study was carried out to obtain an information for the nutritional value of loach Misgurnus mizolepis, as proximate compositions, contribution of nitrogen, amino acids, three lipid classes and their fatty acid compositions. In total nitrogen contents, protein-N was 81.3% and free amino-N was 15.2%. In amino acid compositions of loach, glutamic acid, lysine, arginine and aspartic acid were the main amino acids in quantity. Total lipids of loach consisted of 57.85% of neutral lipids, 15.95% of glycolipids and 26.20.% of phospholipids. In the neutral lipids, the major fatty acids were palmitic acid, palmitoleic acid and oleic acid. The fatty acid composition in the glycolipids was similar to the pattern in tho neutral lipid, but phospholipids contained higher contents of palmitic acid, oleic acid and arachidonic acid than other lipid classes.

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Physicochemical Properties of Domestic Cherry Tomato Varieties (국내산 방울토마토의 이화학적 특성)

  • Ahn, Jun-Bae
    • Culinary science and hospitality research
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    • v.23 no.7
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    • pp.42-49
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    • 2017
  • This study was conducted to determine the nutritional value of domestic cherry tomato varieties (Summerking, Qutiquti, and Minichal). The levels of amino acids, amino acid derivatives, and ${\gamma}-aminobutyric-acid$ (GABA) were analyzed using ion chromatography. In domestic cherry tomatoes, eighteen free amino acids were found including L-glutamic acid (L-Glu), L-glutamine (L-Gln), and L-aspartic acid (L-Asp). L-Glu was the most abundant amino acid, ranging from 1,533.17 mg/100 g to 1,920.65 mg/100 g (dry weight). The next abundant amino acids were L-Gln, ranging from 784.68 mg/100 g to 1,164.36 mg/100 g and L-Asp, ranging from 320.73 mg/100 g to 387.22 mg/100 g. Domestic cherry tomatoes contained eight essential amino acids except tryptophan and the total essential amino acid content was 297.30~432.43 mg/100 g (dry weight), which was 8.92~10.61% of total free amino acid. Several amino acid derivatives were found: L-carnitine (L-Car), hydroxylysine (Hyl), o-phosphoethanolamine (o-Pea), phosphoserine (p-Ser), ${\beta}-alanine$ (${\beta}-Ala$), N-methyl-histidine (Me-His), ethanolamine ($EtNH_2$), and L-citrulline (L-Cit). L-Car, transporting long-chain fatty acid into mitocondrial matrix, was the most abundant amino acid derivative in all domestic cherry tomatoes. A high level of GABA (313.18~638.57 mg/100 g), known as a neurotransmitter, was also found in all three domestic cherry tomatoes. These results revealed that domestic cherry tomatoes have a good balance of nutrient and bioactive compounds. Therefore, cherry tomatoes can be used as a functional food material.

Organoleptic Characteristics of Frankfurter Sausage Made with Venison (사슴육으로 제조한 프랑크푸르터 소시지의 관능적 특성)

  • Kim, Jong-Uk;Baek, Gyung-Gyu;Lee, Nan-Hee;Choi, Won-Seok;Choi, Ung-Kyu
    • The Korean Journal of Food And Nutrition
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    • v.33 no.6
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    • pp.639-644
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    • 2020
  • The purpose of this study was to investigate the quality characteristics of frankfurter sausage made with venison. The crude protein content of sausage showed a significant increase pattern in the proportion to the addition of the venison. The crude fat content showed a decrease pattern. The saturated fatty acid significantly increased in proportion to the content of the venison. The monounsaturated fatty acids and the polyunsaturated fatty acids decreased with increasing the venison content. The content of the free amino acids increased in proportion to the amount of the venison added. The amino acid content of the pork sausage was in the order of Alanine > glycine > glutamic acid, and was similar in the sausages made from venison. The essential amino acids were 36.4% in FSV, higher than 34.0% in the pork sausages. In the sensory test, it was confirmed that the texture, taste, and overall acceptability increased with the addition of the venison. Because the sausages made from the venison were superior to the pork sausages in all of the measured sensory characteristics, it is considered that industrialization is sufficiently feasible.

Physicochemical Characteristics of Traditional Doenjang with added Lentinus edodes (표고버섯을 첨가한 전통된장의 이화학적 특성)

  • Choi Sun-Young;Sung Nak-Ju;Kim Haeng-Ja
    • Korean journal of food and cookery science
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    • v.22 no.1 s.91
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    • pp.69-79
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    • 2006
  • Traditional doenjang with Lentinus edodes added at a concentration of 0, 3, 5 and 10% (w/w) was prepared and its physicochemical characteristics were analyzed during fermentation for 6 months. The moisture content, salt level and pH for doenjang were decreased with increasing addition of Lentinus edodes powder, but crude protein content was increased. Among the free sugars, glucose, galactose, arabinose, sucrose and maltose were identified and quantified, with the first two being the major free sugar. Minerals were detected in the order of Na> K> P> Ca> Mg> Fe while the content of Cu, Mn and Zn exhibited little change. The contents of each amino acid of traditional doenjang with added Lentinus edodes, glutamic, leucine and aspartic acids, had a much higher level. In fatty acid compositions, palmitic, stearic, oleic and linoleic acids were analyzed, with oleic acid being the most abundant fatty acid at $56.4{\pm}0.8\sim57.5{\pm}0.5%$ in all the tested samples. GMP and IMP contents of nucleotides and their related compounds were extremely high. It was thought that these substances contributed to the sweet taste of traditional doenjang with added Lentinus edodes. These results showed the applicability and high quality of traditional doenjang with added Lentinus edodes.

Chemical compositions of Lentinula in growth stage - A study on application plan of Lentinula I - (표고버섯의 생육시기별 성분분석 - 표고버섯의 활용방안 연구 I -)

  • 조덕봉;현규환;나광출;최지호;서재신;강성구;김용두
    • Korean Journal of Plant Resources
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    • v.15 no.2
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    • pp.128-134
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    • 2002
  • Content of crude protein in both pileus and stalk increased progresively as growth period became longer and then decreased at old stage. Contents of ash and reducing sugars followed the same trend as that of the crude protein. However, content of crude lipid decreased as growth progressed more. Seventeen kinds of amino acid were detected in Lentinula. Content of total amino acid increased as mushroom grew more, but decreased after old stage. The amino acid such as Glu, His, Gly and Ala were contained with relatively large amount in Lentinula. Content of free amino acid showed the similar trends as that of total amino acid. Contents of most of the mineral increased as growth period progressed, but K content decreased as growth period became longer. Contents of K and P were much higher than contents of other mineral. The major fatty acid contained in Lentinula were linoleicacid, palmitic acid and oleic acid. The large amount of volatile component in Lentinula was 1-octen-3-ol, so that this material was speculated to be major source of perfume. Other perfumic materials such ethyl acetate, 3-octanone, ethanol, (E)-2-octenal, 4-octen-3-one, acetaldehyde, ethyl formate were also contained in Lentinula, especially in pilous.

Studies on the Chemical Compoments of Wild Korean Lettuce(Youngia sonchifolia Max.) (야생(野生) 고들빼기의 화학성분(化學成分)에 관한 연구(硏究))

  • Shin, Soo-Chull
    • Applied Biological Chemistry
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    • v.31 no.3
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    • pp.261-266
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    • 1988
  • For the effective utilization of Wild Korean lettuce(Youngia sonchifolia Max.), the chemical characteristics, contents of free sugar, amino acid composition, fatty acid composition and minerals were analysed. Free sugars determined from the roots were rhamnose, fructose, glucose, sucrose maltose, melibiose and raffinose, whereas raffinose was not detected from the leaves. Of amino acid composition in the roots glutamic acid content was largest followed by histidine aspartic acid, leucine and proline. As for leaves glutamic acid was highest fellowed by aspartic acid, leucine and histidine. Mtehionine and cystine were found in low content in both roots and leaves. Major fatty acid composition in total lipid(free and bound) were linoleic, linolenic and palmitic acid in both and leaves. P, K and Ca were rich minerals contained in the roots and leaves of Wild Youngia sonchifolia Max.

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The Nutritional Components of Olive Flounder (Paralichthys olivaceus) Fed Diets with Yuza (Citrus junos Sieb ex Tanaka) (유자 첨가 사료로 사육된 넙치의 영양성분)

  • Kim, Heung-Yun;Kim, Eun-Heui;Kim, Do-Hyung;Oh, Myung-Joo;Shin, Tai-Sun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.3
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    • pp.215-223
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    • 2009
  • This study was conducted to investigate the effect of diets supplemented with different levels (0, 2.5, 5.0, and 7.5%) of yuza (Citrus junas Sieb ex Tanaka) on nutritional composition of olive flounder. Four groups of fish (242.2$\pm$14.2 g) were fed to apparent satiation twice daily for 4 months. There were no significant differences in proximate composition among the treatment groups (P<0.05). Vitamin C content in flounder muscle was higher in the yuza-added groups than in the control group, and the content among the treatment groups increased as amount of yuza added to diets increased (P<0.05). Of the eight organic acids in flounder muscle, lactic acid was predominant, followed by oxalic acid, succinic-acid, tartaric acid, and acetic acid. Flounders fed 2.5% yuza diet had the highest lactic acid content of all treatments. Four sugars were found in all groups and glucose was the major sugar. Glucose and ribose were detected as the highest sugars in the 2.5% treatment, while maltose and galactose were the dominant sugars in the 5.0% treatment. The abundant fatty acids in fed flounders were 22:6n-3 (DHA), 16:0, and l8:1n-9, which were composed of over 60% of total fatty acids. The control and the 7.5% treatment group had higher 22:6n-3 (DHA) content than the other groups. Major amino acids in samples were glutamic acid, aspartic acid, lysine, leucine, valine, arginine, and alanine. The 2.5% yuza treatment had the highest content of total amino acids and essential amino acids. There were little differences in the free amino acid compositions among the treatments. However, taurine was the predominant amino acid and made up over 47% of total free amino acids. The 2.5% added yuza group contained higher amount of sweet amino acids such as alanine, serine, proline, glycine than the other groups. The addition of yuza to diet of olive flounder had no or little effect on the nutritional components of olive flounder except for vitamin C. However, the 2.5% yuza added group had the highest nutritional values of the treatment groups.