• Asian-Australasian Journal of Animal Sciences
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• v.32 no.8
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• pp.1095-1103
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• 2019

Objective: Among stress responses, the unfolded protein response (UPR) is a well-known mechanism related to endoplasmic reticulum (ER) stress. ER stress is induced by a variety of external and environmental factors such as starvation, ischemia, hypoxia, oxidative stress, and heat stress. Inositol requiring enzyme $1{\alpha}$ ($IRE1{\alpha}$)-X-box protein 1 (XBP1) is the most conserved pathway involved in the UPR and is the main component that mediates $IRE1{\alpha}$ signalling to downstream ER-associated degradation (ERAD)- or UPR-related genes. XBP1 is a transcription factor synthesised via a novel mechanism called 'frame switch splicing', and this process has not yet been studied in the horse XBP1 gene. Therefore, the aim of this study was to confirm the frame switch splicing of horse XBP1 and characterise its dynamics using Thoroughbred muscle cells exposed to heat stress. Methods: Primary horse muscle cells were used to investigate heat stress-induced frame switch splicing of horse XBP1. Frame switch splicing was confirmed by sequencing analysis. XBP1 amino acid sequences and promoter sequences of various species were aligned to confirm the sequence homology and to find conserved cis-acting elements, respectively. The expression of the potential XBP1 downstream genes were analysed by quantitative real-time polymerase chain reaction. Results: We confirmed that splicing of horse XBP1 mRNA was affected by the duration of thermal stress. Twenty-six nucleotides in the mRNA of XBP1 were deleted after heat stress. The protein sequence and the cis-regulatory elements on the promoter of horse XBP1 are highly conserved among the mammals. Induction of putative downstream genes of horse XBP1 was dependent on the duration of heat stress. We confirmed that both the mechanisms of XBP1 frame switch splicing and various binding elements found in downstream gene promoters are highly evolutionarily conserved. Conclusion: The frame switch splicing of horse XBP1 and its dynamics were highly conserved among species. These results facilitate studies of ER-stress in horse.

• Computational Structural Engineering
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• v.10 no.4
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• pp.165-175
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• 1997

In this paper, the stress, buckling and vibration analyses have been performed for several case with the spot weld stiffened rear side frame, the unstiffened rear side frame and the dual thickness laser weld rear side frame. For stress and vibration analyses, the clamped boundary condition with spring supports are used. But for the buckling analyses, the both ends simply supported boundary conditions are used. For the nummerical analyses, ANSYS 5.0 code is adopted. Maximum stress of the spot weld stiffened rear side frame occurs in the main frame and is 80.9 MPa. Maximum strain is 501 .mu.. The maximum stress of the dual thickness laser weld rear side frame of 1.8mm thickness structure is equal with the stress of spot weld stiffened frame. The weight of dual thickness laser weld frame can be reduced about 17.2%. For the stiffened spot weld rear side frame with both ends simply supported boundary conditon, the bucking load is 52.54 kN. When the thickness of the dual thickness laser weld rear side frame become 1.9mm thickness structure, the buckling load of the stiffenerd rear side frame is equal to that of dual thickness laser weld frame. The reduction of the structure weight is about 5%. The fundamental natural frequency of the stiffened spot weld rear side frame for bending mode is 163.6 Hz and that of the dual thickness laser weld rear side frame is 179.8 Hz.

• Steel and Composite Structures
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• v.2 no.1
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• pp.35-50
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• 2002

A series of tests on simple-welded plate specimens (SWPS) and T-stub tension specimens simulating some of the joint details in moment frame connections were conducted in this investigation. The effects of weld strength mismatch and weld metal toughness on structural behavior of these specimens were considered under both static and dynamic loading conditions. Finite element analyses were performed by taking into account typical weld residual stress distributions and weld metal strength mismatch conditions to facilitate the interpretation of the test results. The major findings are as follows: (a) Sufficient specimen size requirements are essential in simulating both load transfer and constraint conditions that are relevant to moment frame connections, (b) Weld residual stresses can significantly elevate stress triaxiality in addition to structural constraint effects, both of which can significantly reduce the plastic deformation capacity in moment frame connections, (c) Based on the test results, dynamic loading within a loading rate of 0.02 in/in/sec, as used in this study, premature brittle fractures were not seen, although a significant elevation of the yield strength can be clearly observed. However, brittle fracture features can be clearly identified in T-stub specimens in which severe constraint effects (stress triaxiality) are considered as the primary cause, (d) Based on both the test and FEA results, T-stub specimens provide a reasonable representation of the joint conditions in moment frame connections in simulating both complex load transfer mode and constraint conditions.

• Journal of the Korean Magnetics Society
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• v.11 no.2
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• pp.50-57
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• 2001

Magnetic inductive probe was designed and assembled for sensing the residual stress developed in the ferromagnetic thin sheet. The residual stress measurement system with this probe could resolve the residual stresses developed in the sheet in terms of principal stress orientation, and magnitude of the principal stress. It was consumed that the obtained probe output voltage from the qualified ferromagnetic Fe-42Ni lead frame sheet and quality-rejected sheet is effectively determined using the developed device. The lead frame sheet which has accumulated a high level of residual stress always showed a distinctive stress distribution and magnitude compared with those of qualified lead frame sheet. Those differences were well resolved as functions of input current or used frequency.

• Journal of The Korean Digital Architecture Interior Association
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• v.12 no.4
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• pp.67-75
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• 2012

The damage of greenhouse has been increasing due to frequent collapse of frame in greenhouse caused by the heavy snow and strong wind. But, greenhouses are constructed by steel tube members of pipe style and pin connection of them, so these greenhouses are very weak. Therefore, this study was carried out to find the type of member section and structural frame system in stress tolerant greenhouses. The modeling types for analysis were designed in accordance with structural frame configuration and member section in greenhouse. These types of models, which are existing type, diagrid type, symmetric and asymmetric section type of frame member in greenhouse were classified. Displacement analysis varying the vertical and horizontal loads for a series of models was carried out. As a result of this paper, it was verified that the structural frame configuration of diagrid type and asymmetric type of member section is better than existing type in the frame of greenhouses against snow loads and wind loads.

• International journal of advanced smart convergence
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• v.12 no.4
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• pp.443-450
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• 2023

One of the basic tasks in the automobile manufacturing process is to design a bare chassis, which is the basic frame of a vehicle, and a bracket is a member connecting various devices to the frame. Bracket, which is a member connecting the engine, transmission, and suspension, which are the core devices of driving and operating the vehicle, to the frame, must maintain safety during vehicle operation. If the bracket connecting the various devices constituting the vehicle to the frame does not have durability, serious accidents may occur during operation of the vehicle. In this study, we performed stress analysis on the brackets installed in the bare chassis of the 25-passenger bus in the development stage. Based on the stress analysis performed, an improved bracket dimension was proposed.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.8 no.1
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• pp.142-149
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• 1999

A machine frame has been manufactured by casting. However, due to the development of the industrial society, 3-D duties was refused. Especially, the declination of the casting industry makes it difficult to produce the frame. Many companies still manufacture the small casting products. The large casting products are extremely limited and manufactured for their own use. Therefore, it is difficult to keep the term of order. In this study, the characteristics of steel structure which is produced by welding were identified in the view of mechanical strength of steel structure which is produced by welding were identified in the view of mechanical strength to substitute steel structure for casting frame. But welding structure has the residual stress, HAZ and welding deformation. Residual stress and HAZ especially cause crack growth. The proposed steel structure, based on the simulation and experiment(tensile curve and S-N curve), can reduce not only the producting term but also the weight of the frame.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.7 no.3
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• pp.104-109
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• 1998

This document is a experimental study on fatigue life of brace mounting weld joint. A brace is used to put sub-frame together on the main frame with high strength bolts. It has low fatigue life so a patch is in need for improving a fatigue characteristics of welded joint in brace mounting This paper presents the most pertinent patch size for truck. For this, a critical stress is computed at the point of fatigue crack occurred on truck frame by finite Element Analysis. Using by this critical stress. Designers are able to determin whether fatigue crack is occurred and are able to select a pertinent patch type. And then, with a selected patch type, structural joint stiffness was estimated to compare to the conventional and other patch type or brace mounting Finally, fatigue test were performed to prove a suitability of selected prototype compare with the conventional and other patch type or brace mounting.

• Journal of the Korean Society of Safety
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• v.26 no.2
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• pp.1-5
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• 2011

In order to evaluate fatigue endurance for an ultra-light weight inline skate frame, FEM analysis was performed. Tensile properties and a S-N curve were determined through tensile and fatigue tests on a modified Al-7075+$S_c$ alloy. The yield and ultimate tensile strengths were 553.3 MPa and 705.5 MPa, respectively. The fatigue endurance limit of this alloy was 201.2 MPa. For evaluating the fatigue endurance of the inline skate frame, the S-N data were compared with the stress analysis results through FEM analysis of the frame. The maximum Von-Mises stress of the frame was determined 106 MPa through FEM analysis of the frame, assuming that the rider weight is 75 Kg. Conclusively, on the basis of fatigue limit, the inline skate frame has a safety factor of approximately 2.0.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.1
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• pp.57-62
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• 2013

Because there is no suspension and differential devices at cart body, the deformation of the frame happened during kart driving affects the driving performance caused by the elastic deformation and the fatigue life of kart frame resulted from the permanent deformation. The dynamic behavior of kart caused by the torsional deformation during circular driving is the important factor of these two kinds of deformations. In order to analyze the dynamic behavior of kart at this curved section, GPS is used to trace the track of kart and the torsional stress at kart-frame has been measured with real time. The mechanical properties of kart-frames for leisure and racing are investigated through material property analysis and tensile test. Torsional stress concentration and frame distortion are investigated through stress analysis on frame on the basis of study result. The real karts for leisure and racing kart are also tested in each driving condition by using the driving analysis equipment. The driving behavior of kart at the curved section are investigated through this test. As the phenomenon of load movement due to centrifugal force at car is happened during circular driving, the torsional stress occurs at cart steel frame.