• The Journal of Korean Institute of Communications and Information Sciences
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• v.26 no.12A
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• pp.2027-2035
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• 2001

In this paper, we present an audio stream delivery using the AMR (Adaptive Multi-Rate) coder that was adopted by ETSI and 3GPP as a standard vocoder for next generation IMT-2000 service in which includes combined sender (FEC) and receiver reconstruction technique in the Internet. By use of the media-specific FEC scheme, the possibility to recover lost packets can be much increased due to the addition of repair data to a main data stream, by which the contents of lost packets can be recovered. The AMR codec is based on the code-excited linear predictive (CELP) coding model. So we use a frame erasure concealment for CELP-based coders. The proposed scheme is evaluated with ITU-T G.729 (CS-ACELP) coder and AMR - 12.2 kbit/s through the SNR (Signal to Noise Ratio) and the MOS (Mean Opinion Score) test. The proposed scheme provides 1.1 higher in Mean Opinion Score value and 5.61 dB higher than AMR - 12.2 kbit/s in terms of SNR in 10% packet loss, and maintains the communicab1e quality speech at frame erasure rates lop to 20%.

• Journal of Broadcast Engineering
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• v.13 no.3
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• pp.310-318
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• 2008

The H.264/AVC standard achieves higher coding efficiency than previous video coding standards with the rate-distortion optimization (RDO) technique which selects the best coding mode and reference frame for each macroblock. As a result, the complexity of the encoder have been significantly increased. In this paper, a fast intra-mode decision algorithm is proposed to reduce the computational load of intra-mode search, which is based on the inverse tree-structure edge prediction algorithm. First, we obtained the dominant edge for each $4{\times}4$ block from local edge information, then the RDO process is only performed by the mode which corresponds to dominant edge direction. Then, for the $8{\times}8$ (or $16{\times}16$) block stage, the dominant edge is calculated from its four $4{\times}4$ (or $16{\times}16$) blocks' dominant edges without additional calculation and the RDO process is also performed by the mode which is related to dominant edge direction. Experimental results show that proposed scheme can significantly improve the speed of the intra prediction with a negligible loss in the peak signal to noise ratio (PSNR) and a little increase of bits.

• The KIPS Transactions:PartB
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• v.9B no.3
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• pp.351-358
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• 2002

Video compression based on DCT (Discrete Cosine Transform) has weakpoints of blocking artifacts and pixel loss when the resolution is changed. DWT (Discrete Wavelet Transform) based method can overcome such problems. In SAMCoW (Scalable Adaptive Motion Compensation Wavelet), one of wavelet based video coding algorithm, both intra frames and motion compensated error frames are encoded using EZW(Embedded Zerotree Wavelet) algorithm. However the property of wavelets transform coefficients of motion compensated error frames are different from that of still images. Signal energy is not highly concentrated in the lower bands which is true for most still image cases. Signal energy is rather evenly distributed over all frequency bands. This paper suggests a new video coding algorithm utilizing these properties. Spatial band coding which is known to be very effective for encoding images with relative1y high frequency components and not utilizing the interband coefficients correlation is applied instead of EZW to encode both intra and inter frames. In spatial band coding, the position and value of significant wavelet coefficients in each band are progressively transmitted. Unlike EZW, inter band coefficients correlations are not utilized in spatial band coding. It has been shown that spatial band coding gives better performance than EZW when applied to wavelet based video compression.

• Journal of Sericultural and Entomological Science
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• v.52 no.1
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• pp.45-51
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• 2014

A cecropin-A gene was isolated from the immunized larvae of the Japanese oak silkworm, Antheraea yamamai and designed Ay-CecA. The complete Ay-CecA cDNA consists of 419 nucleotides with 195 bp open reading frame encoding a 64 amino acid precursor that contains a putative 22-residue signal peptide, a 4-residue propetide and a 37-residue mature peptide with a theoretical mass of 4046.81. The deduced amino acid sequence of the peptide evidenced a significant degree of identity (62 ~ 78% identity) with other lepidopteran cecropins. Like many insect cecropin, Ay-CecA also harbored a glycine residue for C-terminal amidation at the C-end, which suggests potential amidation. To understand this peptide better, we successfully expressed bioactive recombinant Ay-CecA in Escherichia coli that are highly sensitive to the mature peptide. For this, we fused mature Ay-CecA gene with insoluble protein ketosteroid isomerase (KSI) gene to avoid the cell death during induction. The fusion KSI-CecA protein was expressed as inclusion body. The expressed fusion protein was purified by Ni-NTA immobilized metal affinity chromatography (IMAC), and cleaved by cyanogen bromide (CNBr) to release recombinant Ay-CecA. The purified recombinant Ay-CecA showed considerably antibacterial activity against Gram-negative bacteria, E. cori ML 35, Klebsiella pneumonia and Pseudomonas aeruginosa. Our results proved that this peptide with a potent antibacterial activity may play a role in the immune response of Japanese oak silkworm.

• Korean journal of applied entomology
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• v.45 no.1 s.142
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• pp.15-24
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• 2006

Inhibitor kB (IkB)-like gene has been found in the genome of Cotesia plutellae bracovirus (CpBV), which is the obligatory symbiont of an endoparsitoid wasp, C. plutellae. The open reading frame of CpBV-IkB was 417 bp and encoded 138 amino acids. Four ankyrin repeat domains were found in CpBV-IkB, which shared high homology with other known polydnavirus IkBs. Considering a presumptive cellular IkB based on Drosophila Cactus, CpBV-IkB exhibited a truncated structure with deletion of signal-receiving domains, which suggested its irreversible inhibitory role in NFkB signal transduction pathway of the parasitized host in response to the wasp parasitization. CpBV-IkB was expressed only in the parasitized diamondback moth, Plutella flostella. Its expression was estimated by quantitative RT-PCR during parasitization period, showing a constitutive expression pattern from the first day of parasitization. An indirect functional analysis of CpBV-IkB was conducted and suggested a hypothesis of host antivirus inhibition.

• Journal of the Institute of Electronics and Information Engineers
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• v.51 no.4
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• pp.173-180
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• 2014

Compressive sensing is a new signal acquisition paradigm that enables sparse/compressible signal to be sampled under the Nyquist-rate. To fully benefit from its much simplified acquisition process, huge efforts have been made on improving the performance of compressive sensing recovery. However, concerning color images, compressive sensing recovery lacks in addressing image characteristics like energy distribution or human visual system. In order to overcome the problem, this paper proposes a new group-sparsity hard thresholding process by preserving some RGB-grouped coefficients important in both terms of energy and perceptual sensitivity. Moreover, a smoothed group-sparsity iterative hard thresholding algorithm for compressive sensing of color images is proposed by incorporating a frame-based filter with group-sparsity hard thresholding process. In this way, our proposed method not only pursues sparsity of image in transform domain but also pursues smoothness of image in spatial domain. Experimental results show average PSNR gains up to 2.7dB over the state-of-the-art group-sparsity smoothed recovery method.

• Parasites, Hosts and Diseases
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• v.34 no.2
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• pp.135-142
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• 1996

Antigenic domain of jai or surface protein (p30) of Toxoplosmc Sondii was analyzed after polymerase chain reaction (PCR) of its gene fragments. Hydrophilic or hydrophobic moiety of amino acid sequences were expressed as glutathione S-transferase (G57) fusion proteins. Fragments of p30 gene were as follows: 737, total p30 open reading frame (ORF) ; S28, total ORF excluding N-terminal signal sequence and C-terminal hydrophobic sequence; Al9, N-terminal 2/3 parts of A28; A19, N-terminal 2/3 of S28; P9, C-terminal 2/3 part of S28; Z9. middle 1/3 of S28; and 29, C-terminal 1/3 of S28. respectively. Primer of each fragment was synthesized to include clamp sequence of EcoR I restriction site. PCR amplified DNA was inserted info GST (26 kDa) expression vector, PGEX-47-1 to transform into Escheri,hia coei (.JM105 strain). G57 fusion proteins were expressed with IPTG induction as 63. 54, 45, 45, 35, 36. and 35 kDa proteins measured by SDS-PAGE. Each fusion protein was confirmed with G57 detection kit. Western blot analysis with the serum of a toxoplasmosis patient revealed antigenicity in proteins expressed by T37. S28, and Al9 but not those by Pl8. X9, Y10, and Z9. Antigenicity of p30 seems to be located either in N-terminal 115 part in the presence of middle 1/3 part or in the oligopeptides between margins of the first and second 1/3 parts.

• BMB Reports
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• v.41 no.2
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• pp.170-175
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• 2008

In protein therapy, it is important for exogenous protein to be delivered into the target subcellular localization. To transduce a therapeutic protein into its specific subcellular localization, we synthesized nuclear localization signal (NLS) and membrane translocation sequence signal (MTS) peptides and produced a genetic in-frame SOD fusion protein. The purified SOD fusion proteins were efficiently transduced into mammalian cells with enzymatic activities. Immunofluorescence and Western blot analysis revealed that the SOD fusion proteins successfully transduced into the nucleus and the cytosol in the cells. The viability of cells treated with paraquat was markedly increased by the transduced fusion proteins. Thus, our results suggest that these peptides should be useful for targeting the specific localization of therapeutic proteins in various human diseases.

• Plant Biotechnology Reports
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• v.3 no.2
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• pp.147-155
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• 2009

Oryza grandiglumis Chitinase IVa (OgChitIVa) cDNA encoding a class IV chitinase was cloned from wild rice (Oryza grandiglumis). OgChitIVa cDNA contains an open reading frame of 867 nucleotides encoding 288 amino acid residues with a predicted molecular weight of 30.4 kDa and isoelectric point of 8.48. Deduced amino acid sequences of OgChitIVa include the signal peptide and chitin-binding domain in the N-terminal domain and conserved catalytic domain. OgChitIVa showed significant similarity at the amino acid level with related monocotyledonous rice and maize chitinase, but low similarity with dicotyledoneous chitinase. Southern blot analysis showed that OgChitIVa genes are present as two copies in the wild rice genome. It was shown that RNA expression of OgChitIVa was induced by defense/stress signaling chemicals, such as jasmonic acid, salicylic acid, and ethephon or cantharidin and endothall or wounding, and yeast extract. It was demonstrated that overexpression of OgChitIVa in Arabidopsis resulted in mild resistance against the fungal pathogen, Botrytis cinerea, by lowering disease rate and necrosis size. RT-PCR analysis showed that PR-1 and PR-2 RNA expression was induced in the transgenic lines. Here, we suggest that a novel OgChitIVa gene may play a role in signal transduction process in defense response against B. cinerea in plants.

• Journal of the Institute of Convergence Signal Processing
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• v.17 no.1
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• pp.10-17
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• 2016

GPS location information storage included in the HD black box is using a unique manner for each manufacturer does not have a specific standard. In this paper, in order to overcome the limitations of the storage space and thereby the image quality according to store GPS position information deteriorate to solve the problems that cause, we propose the location information concealment method included in the HDTV video content using a essential hidden region. HDTV video content is a Border Extender of 8 lines in the frame to the bottom of the compression will be required. This was inserted into the image of a gray scale used in block form in order to space the current position information is concealed to prevent image degradation. The proposed method was confirmed using real HD black box, there are more difficult to interpret the format of the ASCII code re-edit the location information when the compression effect disappears with the existing security zones added. Therefore, the proposed method is suitable for location-based services, such as Facebook or Youtube videos.