• Asian-Australasian Journal of Animal Sciences
• /
• v.18 no.5
• /
• pp.626-631
• /
• 2005

Nitric oxide (NO) derived from inducible nitric oxide synthase (iNOS) is involved in cell apoptosis, which contributes to luteal regression and luteolysis in some species. In large domestic animals, no direct evidence for the relationship between NO and cell apoptosis in the process of corpus luteum regression is reported. The present study was conducted to investigate the localization of iNOS on porcine corpora lutea (CL) during the oestrus cycle and its relation to cell DNA fragmentation and CL regression. According to morphology, four luteal phases throughout the estrous cycle were defined as CL1, CL2, CL3 and CL4. By isoform-specific antibody against iNOS, the immunochemial staining was determined. Luteal cell DNA fragmentation was determined by flow cytometry. The results showed that no positive staining for iNOS was in CL1 and that iNOS was produced but limited to the periphery of CL2, while in the CL3, the spreading immunochemical staining was found inside the CL. No iNOS positive staining was detected in CL4. Meanwhile, DNA fragmentation increased dramatically when CL developed from CL2 to CL3 (p<0.05). In CL4, higher proportion of luteal cells still had fragmented DNA than that of luteal cells from CL1 or CL2 (p<0.05). These results indicate that iNOS expression is closely related to luteal cell apoptosis and then to luteal regression.

• Tunnel and Underground Space
• /
• v.15 no.4 s.57
• /
• pp.264-274
• /
• 2005

The optimum blasting pattern to excavate a quarry efficiently and economically can be determined based on the minimum production cost which is generally estimated according to rock fragmentation. Therefore it is a critical problem to predict fragment size distribution of blasted rocks over an entire quarry. By comparing various prediction models, it can be ascertained that the result obtained from Kuz-Ram model relatively coincides with that of field measurements. Kuz-Ram model uses the concept of rock factor to signify conditions of rock mass such as block size, rock jointing, strength and others. For the evaluation of total production cost, it is imperative to estimate 3-D spatial distribution of rock factor for the entire quarry. In this study, a sequential indicator simulation technique is adopted for estimation of spatial distribution of rock factor due to its higher reproducibility of spatial variability and distribution models than Kriging methods. Further, this can reduce the uncertainty of predictor using distribution information of sample data The entire quarry is classified into three types of rock mass and optimum blasting pattern is proposed for each type based on 3-D spatial distribution of rock factor. In addition, plane maps of rock factor distribution for each ground levels is provided to estimate production costs for each process and to make a plan for an optimum blasting pattern.

• Journal of the Korean Association of Geographic Information Studies
• /
• v.22 no.1
• /
• pp.168-182
• /
• 2019

The objectives of this study were to examine the production processes and methods of "Forest Type Map Actualization Production (Database (DB) Construction Work Manual)" (Work Manual) identify issues associated with the production processes and methods, and suggest solutions for them by applying evaluation items to a 1:5k digital forest type map. The evaluation items applied to a forest type map were divided into zoning and attributes, and the issues associated with the production processes and methods of Work Manual were derived through analyzing the characteristics of the stand structure and fragmentation by administrative districts. Korea is divided into five divisions, where one is set as the area changed naturally and the other four areas set as the area changed artificially. The area changed naturally has been updated every five years, and those changed artificially have been updated annually. The fragmentation of South Korea was analyzed in order to examine the consistency of the DB established for each region. The results showed that, in South Korea, the number of patches increased and the mean patch size decreased. As a result, the degree of fragmentation and the complexity of shapes increased. The degree of fragmentation and the complexity of shapes decreased in four regions out of 17 regions (metropolitan cities and provinces). The results indicated that there were spatial variations. The "Forest Classification" defines the minimum area of a zoning as 0.1ha. This study examined the criteria for the minimum area of a zoning by estimating the divided object (polygon unit) in a forest type map. The results of this study revealed that approximately 26% of objects were smaller than the minimum area of a zoning. The results implied that it would be necessary to establish the definition and the regeneration interval of "Areas Changed Artificially and Areas Changed Naturally", and improve the standard for the minimum area of a zoning. Among the attributes of Work Manual, "Species Change" item classifies terrain features into 52 types, and 43 types of them belong to stocking land. This study examined distribution ratios by extracting species information from the forest type map. It was found that each of 23 species, approximately 53% of species, occupied less than 0.1% of Forested land. The top three species were pine and other species. Although undergrowth on unstocked forest land are classified in the terrain feature system, their definition and classification criteria are not established in the "Forest Classification" item. Therefore, it will be needed to reestablish the terrain feature system and set the definitions of undergrowth.

• Journal of the Economic Geographical Society of Korea
• /
• v.19 no.3
• /
• pp.491-511
• /
• 2016

This paper attempts to understand the nature and dynamics of global value chains in the auto industry using the OECD TiVA 2015 edition on the bilateral foreign value added in exports over the period 1995-2011 and employing the techniques of social network analysis such as the computation of network measures and visualization of value added trade flows. It is shown that there has been a tendency towards increasing production fragmentation both within and across regions. The automotive value-added network is found to have small-world properties with a hierarchical, clustered and dense structure. The differences among the US, Germany and China as major suppliers of foreign value added in global automotive value chains are remarkably revealed. Although the fragmentation of production has been developed on a global scale, a dichotomous tension between center and periphery and domestic and foreign capital lies behind it.

• Journal of the Economic Geographical Society of Korea
• /
• v.27 no.1
• /
• pp.53-73
• /
• 2024

While global production supply chains are expected to shift toward friendly or allied countries, pandemic-shoring such as nearshoring and friendshoring, due to increased protectionism and the reorganization of regional value chains in the wake of the global pandemic, the study found that economic rather than political factors are still at play in consumer supply chains. In the case of Chinese e-commerce market (C-commerce), the study attributes the rapid growth of the market to (1) related regulatory relaxations introduced to stimulate consumption after the end of COVID-19, (2) an increasing pattern of wanting to consume more for a limited income and (3) unconventional business activities to increase their share of the global consumption market. Through these phenomena, the production-consumption network is likely to develop into a more fragmented form, and the consumption network in particular is expected to become more fragmented, influenced by the digital technology war, a phenomenon of re-globalization.

• The Korea Journal of Herbology
• /
• v.29 no.6
• /
• pp.63-71
• /
• 2014

Objectives : Lonicerae japonicae Flos(LJF) has been reported to exhibit anti-oxidant, anti-inflammatory, anti-viral, anti-rheumatoid properties. However, it is still largely unknown whether LJF inhibits the ultraviolet(UV)B-induced oxidative damage in human HaCaT keratinocytes. Therefore in this paper, we investigated the anti-oxidative capacity and protective effect of LJF against UVB-induced oxidative demage in human HaCaT keratinocytes. Methods : To evaluate the anti-oxidative activity of LJF extracts, we measured total phenolic contents, total flavonoid contents, antioxidant capacity, and superoxide scavenging activity. To give an oxidative stress to HaCaT cells, UVB was irradiated with $200mJ/cm^2$ to HaCaT cells. To detect the protective effect of LJF against UVB, we measured cell viability, DNA fragmentation and reactive oxygen species (ROS) production. In addition, we performed high-performance liquid chromatography (HPLC) analysis to find a major component of LJF. Results : LJF contained phenolic and flavonoid contents, and showed the anti-oxidant and superoxide scavenging activity. The UVB-induced oxidative conditions led to the cell death, DNA fragmentation and reactive oxygen species (ROS) production. However, pretreatment with LJF reduced oxidative conditions, including inhibition of cell death, DNA fragmentation and ROS production. In addition, we found out chlorogenic acid as major component of LJF. Conclusions : These results could suggest that LJF contained anti-oxidative contents and exhibited protective effects against UVB on human HaCaT keratinocytes. And the effective compound of LJF which could show protective activities against UVB is chlorogenic acid. Thus, LJF and chlorogenic acid would be useful for the development of drug or cosmetics treating skin troubles.

• 한국해양학회지
• /
• v.3 no.1
• /
• pp.8-15
• /
• 1968

An interesting bacterium (#271) was isolated from the abyssal plain of the Sigsbee Deep of the Gulf of Mexico. The organism exhibits marked polymorphism (baciloid, coryneform and myceloid morphologies) in response to certain cultural conditions. the organism has been observed undergoing reproduction by transverse fission, fragmentation and arthrospore production. The presence of arthrospores indicates the bacterium is a member of the genus Arthrobacter; however, computed affinity coefficients do not confirm this genus. Until further studies have been completed on this isolate the authors are reluctant to place it in a generic group.

• Asian-Australasian Journal of Animal Sciences
• /
• v.32 no.10
• /
• pp.1611-1620
• /
• 2019

Objective: The aim was to investigate the influence of ultrasound and adenosine 5'-monophosphate (AMP) marination (UAMP) on tenderness and structure of myofibrillar proteins of beef. Methods: Five groups, the untreated meat (Control), deionized water marination (DW), ultrasound followed by DW (UDW), AMP marination (AMP), and ultrasound followed by AMP (UAMP) were studied. Myofibrillar fragmentation, cooking loss, shear force, thermograms, histological observation of meats and myofibrillar proteins properties were investigated in these different treatments. Results: The results showed that UAMP significantly increased myofibrillar fragmentation index from 152 (Control), 231 (AMP), and 307 (UDW) to 355 (p<0.05), respectively. The lowest cooking loss, shear force and peak denaturation temperature were observed in UAMP. In histological observation, UDW and UAMP had more fragmented muscular bundles than the others. Furthermore, a drastic increase in ${\alpha}$-helix and decrease in ${\beta}$-sheet of myofibrillar proteins was observed in UAMP, implying the disaggregation of protein samples. The synchronous fluorescence spectra of myofibrillar proteins in UAMP suggested the combination of ultrasound and AMP could accelerate the unfolding molecular structure and destroying hydrophobic interactions. The results of circular dichroism and synchronous fluorescence spectra for myofibrillar proteins coincided with the microstructures of beef. Conclusion: The results indicate that ultrasound combined with AMP improved meat tenderness not only by disruption in muscle integrity, increasing water retention, but also altering their spatial structure of myofibrillar proteins.

• Asian-Australasian Journal of Animal Sciences
• /
• v.22 no.4
• /
• pp.483-491
• /
• 2009

In vitro produced porcine embryos have potential application in reproductive biotechnology. However, their development potential has been very low. This study evaluated the in vitro developmental ability and quality of cloned and parthenogenetic porcine embryos having 2-4 cells or 5-8 cells on Day 2 of in vitro culture. Analysis of results showed that 2 to 4 cell embryos had higher ability to form blastocysts than 5 to 8 cell embryos (p<0.05). Blastocysts produced from culture of 2 to 4 cell embryos also contained higher cell numbers and had lower BAX:BCLxL transcript ratio than those produced from 5 to 8 cell embryos (p<0.05), thereby suggesting 2 to 4 cell embryos have higher development potential. Further investigation revealed that 5 to 8 cell embryos had higher incidence (100${\pm}$0.0%) of blastomeric fragmentation than 2 to 4 cell embryos (15.2${\pm}$5.5% for parthenogenetic and 27.7${\pm}$7.1% for cloned embryos). This suggests that low development potential of 5 to 8 cell embryos was associated with blastomeric fragmentation. In conclusion, we have shown that morphological selection of embryos based on cell number on Day 2 of in vitro culture could offer a practical and valuable non-invasive means to select good quality porcine embryos.

• Korean Journal of Animal Reproduction
• /
• v.26 no.4
• /
• pp.385-394
• /
• 2002

This study was constructed the correlations of the embryonic developmental rates and the frequency of chromosome aberration using ear-skin-fibroblast cell in nuclear transfer (NT) derived embryos. Karyoplast-oocyte complexes were fused and activated simultaneously, then cultured for seven days to assess development. The developmental rates of NT and in vitro fertilization (IVF) embryos were 55.4% vs 63.5%, 31.7% vs 33% and 13.4% vs 16.8% in 2 cell, 8 cell and blastocyst, respectively. Firstly, the frequency of chromosome aberrations were evaluated using fluorescent in situ hybridization (FISH) technique with porcine chromosome 1 submetacentric specific probe. Chromosome aberration was detected at day 3 on the embryo culture, the percentages of chromosomal aneuploidy in NT and IVF embryos at 4-cell stage were 40%, 31.3%, respectively. Secondly, embryonic fragmentation was evaluated at 4-cell stage embryo. Frequency of embryonic fragmentations was in 51.3% of NT, 61.3% of IVF, 28.9% of parthenogenetic activation at 4-cell stage. The proportion of fragmentation in NT embryos was higher than activation embryos. This result indicates that chromosomal abnormalities and embryonic fragments are associated with low developmental rate in porcine NT embryo. It is also suggest that abnormal porcine embryos produced by NT related with lower implantation rate, increased abortion rate and production of abnormal fetuses.