• Food Science and Biotechnology
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• v.17 no.5
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• pp.976-982
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• 2008

The 10 species of marine green algae was collected from Jejudo(Island) in Korea. Methanolic and aqueous extracts were prepared and screened for inhibition activities against tumor cell growth. Of the tested samples, the sea staghorn (Codium fragile) aqueous extract (CFAE) showed the highest activity on CT-26 cell growth. Therefore, CFAE was selected for further experiments and the possibility to induce apoptosis by the CFAE was investigated. Flow cytometric analysis revealed that it dose-dependently increased apoptotic cells with hypodiploid DNA contents in CT-26 cell line. These results indicated that CFAE can suppress the growth of CT-26 cells through apoptosis. The CFAE decreased the protein expression of anti-apoptotic Bcl-xL and led to the activation of caspase-3 and -7. A crude polysaccharide was separated from CFAE and it mainly constituted with 61.2% galactose and 30.5% arabinose as analyzed by high performance liquid chromatography (HPLC).

• Journal of Korea Multimedia Society
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• v.7 no.9
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• pp.1294-1303
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• 2004

This paper proposes new block fragile watermarking for image authentication and integrity by using the existing pixel-based scheme and block-based scheme. The proposed scheme is performed as fellows. First, we choose LUT(Look Up Table) from each pixel of original image and information of the corresponding block. Next, we insert a watermark, modifying original image with values to compare binary original image with the watermark to be embedded. As a result, we provide the means to overcome some weakness of the existing scheme. Binary logo as watermark can be detected from watermarked image and altered location can also be detected by the unit of pixel or that of block in our scheme.

• Journal of Information Processing Systems
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• v.13 no.2
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• pp.385-399
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• 2017

Nowadays, with the development of signal processing technique, the protection to the integrity and authenticity of images has become a topic of great concern. A blind image authentication technology with high tamper detection accuracy for different common attacks is urgently needed. In this paper, an improved fragile watermarking method based on local binary pattern (LBP) is presented for blind tamper location in images. In this method, a binary watermark is generated by LBP operator which is often utilized in face identification and texture analysis. In order to guarantee the safety of the proposed algorithm, Arnold transform and logistic map are used to scramble the authentication watermark. Then, the least significant bits (LSBs) of original pixels are substituted by the encrypted watermark. Since the authentication data is constructed from the image itself, no original image is needed in tamper detection. The LBP map of watermarked image is compared to the extracted authentication data to determine whether it is tampered or not. In comparison with other state-of-the-art schemes, various experiments prove that the proposed algorithm achieves better performance in forgery detection and location for baleful attacks.

• Proceedings of the IEEK Conference
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• 2003.07e
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• pp.2411-2414
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• 2003

In this paper, we present a digital image authentication using semi-fragile watermarking techniques. The algorithm is robust to innocuous manipulations while detecting malicious manipulations. Specifically, the proposed method is designed for the purpose of the real time authentication of an image frame captured from a digital camera due to its easy H/W implementation, security and visible verification. To achieve the semi-fragile characteristics that survive a certain amount of compression, we employ the invariant property of DCT coefficients' quantization proposed by Lin and Chang [1]. The binary watermark bits are generated by exclusive ORing the binary logo with pseudo random binary sequences. Then watermark bits are embedded into the LSBs of pre-quantized DCT coefficients in the medium frequency range. Verification is carried out easily due to visually recognizable pattern of the logo extracted by exclusive ORing the LSBs of the embedded DCT coefficient with pseudo random number seeded by a secret key. By the experiment results, this method is not only robust to JPEG compression but also it detects powerfully alterations of the original image, such as the tempering of images.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.45 no.5
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• pp.1-7
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• 2008

This paper presents not only the robust stabilization technique but also robust non-fragile controller design method for discrete-time singular systems and static state feedback controller with multiplicative uncertainty. The condition for the existence of robust stabilization controller, the admissible controller design method, and the measure of non-fragility in controller are proposed via LMI(linear matrix inequality) approach. In order to get the maximum measure of non-fragility, the obtained sufficient condition can be rewritten as LMI optimization form in terms of transformed variable. Therefore, the presented robust non-fragile controller for discrete-time singular systems guarantees robust stability in spite of parameter uncertainty and controller fragility. Finally, a numerical example is given to show the validity of the design method.

• BMB Reports
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• v.39 no.6
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• pp.766-773
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• 2006

Fragile-X-syndrome (FXS) is the most common type of inherited cognitive impairment. The underlying molecular alteration consists of a CGG-repeat amplification within the FMR-1 gene. The phenotype is only apparent once a threshold in the number of repeats has been exceeded (full mutation). The aim of this study was to characterize the FMR-1 CGG-repeat status in Argentine patients exhibiting mental retardation. A total of 330 blood samples from patients were analyzed by PCR and Southern blot analysis. Initially, DNA from 78 affected individuals were studied by PCR. Since this method is unable to detect high molecular weight alleles, however, we undertook a second approach using the Southern blotting technique to analyze the CGG repeat number and methylation status. Southern blot analysis showed an altered pattern in 14 out of 240 (6%) unrelated patients, with half of them presenting a mosaic pattern. Eight out of 17 families (47%) showed a (suggest deleting highlight). The characteristic FXS pattern was identified in 8/17 families (47%), and in 4 of these families 25% of the individuals presented with a mosaic model. The expansion from pre-mutation to full mutation was shown to occur both at the pre and post zygotic levels. The detection of FXS mutations has allowed us to offer more informed genetic counseling, prenatal diagnosis and reliable patient follow-up.

• Journal of the Korean Society of Tobacco Science
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• v.8 no.1
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• pp.13-21
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• 1986

The fragility, which express the fineness index, increased linearly with the moisture content for various tobacco shreds and showed a high correlation to the moisture content. Then, fineness index (FIs) at reference moisture content(l2.5%) was obtained by following equation. FIs=FIm+A(12.5-Mm) A" denote the gradient of simple linear regression equation between the fineness index and moisture content. FIm; the fineness index measured at the standard moisture content(12.5%). The characteristics of fragility concerning with some kinds of tobacco raw materials, stalk position, moisture content and storaged time were investigated. The following results were obtained. 1. The moisture content range of less fragile of tobacco raw material was from 16% to 20%. whereas the rolled process sheet tobacco was from 12% to 16%. 2. The Bright yellow and the paper process sheet tobacco were less fragile than that of the other tobacco raw materials. 3. The leaves were more distant from middle stalk position, and its become more fragile. 4. Physical properties of the storaged tobacco leaves were determined as a function of each storageunction of each storage time. The long-term storaged leaves(more than 3 years) were slightly lighter weight, more fragility and lower equilbrium moisture content than thos of the mid-term storaged leaves.(less than 3 years). 5. Contents of chemical component of the storaged leaves were determined every storage time. The long-term storaged leaves were slightly lower content of nicotine and chlorine than those of mid-term storaged leaves, but contents of crude fiber, total ash and calcium were increased significantly.

• Journal of Information Processing Systems
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• v.14 no.3
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• pp.666-679
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• 2018

The discrete wavelet transform (DWT) has good multi-resolution decomposition characteristic and its low frequency component contains the basic information of an image. Based on this, a fragile watermarking using the local binary pattern (LBP) and DWT is proposed for image authentication. In this method, the LBP pattern of low frequency wavelet coefficients is adopted as a feature watermark, and it is inserted into the least significant bit (LSB) of the maximum pixel value in each block of host image. To guarantee the safety of the proposed algorithm, the logistic map is applied to encrypt the watermark. In addition, the locations of the maximum pixel values are stored in advance, which will be used to extract watermark on the receiving side. Due to the use of DWT, the watermarked image generated by the proposed scheme has high visual quality. Compared with other state-of-the-art watermarking methods, experimental results manifest that the proposed algorithm not only has lower watermark payloads, but also achieves good performance in tamper identification and localization for various attacks.

• Earthquakes and Structures
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• v.22 no.1
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• pp.15-23
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• 2022

One of the key tools in assessing the seismic vulnerability of the structures is the use of fragile functions, which is the possibility of damage from a particular damage surface for several levels of risk from the seismic movements of the earth. The aim of this study is to investigate the effect of two categories of earthquake events on the fragile curve (FRC) of the steel construction system. In this study, the relative lateral displacement of the structures is considered as a damage criterion. The limits set for modifying the relative lateral position in the HAZUS instruction are used to determine the failure modes, which include: slight, moderate, extensive and complete. The results show, as time strong-motion increases, the probability of exceeding (PoE) increases (for Peak ground acceleration (PGA) less than 0.5). The increase in seismic demand increases the probability of exceeding. In other words, it increases the probability of exceeding, if the maximum earthquake acceleration increases. Also, 7-storey model in extensive mode has 20 and 26.5% PoE larger than 5- and 3-storey models, respectively.

• Journal of Life Science
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• v.26 no.4
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• pp.484-489
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• 2016

Living organisms can maintain or extend their territories by producing allelochemicals that influence the growth, survival, and reproduction of other organisms. To identify natural biostimulants of positive allelochemicals, we screened 18 common seaweed extracts for enhancement of rhizoid and blade production in a convenient Porphyra suborbiculata monospore assay. By addition of methanolic extract from the most potent green seaweed, Codium fragile, 100% and 50% enhancement doses reflecting the amount of C. fragile extract required to enhance rhizoid formation (in terms of number of spores with rhizoids per total spores tested) were approximately 100 and 50 μg/ml, respectively, in the P. suborbiculata monospore culture. The C. fragile extract quickly enhanced rhizoid formation, rhizoid numbers per rhizoid-holding spore, rhizoid length, blade formation (in number of spores with blade per total spores tested), and blade length from most monospores at early culture days. The extract enhanced rhizoid formation after 2 days of culture significantly, rhizoid numbers per rhizoid-holding spore after 3 days, rhizoid length after 3 days, blade formation after 2 days, and blade length after 1 day, respectively, from most monospores. The allelochemicals that enhanced favorite seaweed species may be efficacious for new seaweed management technologies, including the development of biostimulant agents based on natural products.