• 한국식품영양과학회지
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• 제31권5호
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• pp.899-904
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• 2002

알로에 및 프로폴리스 추출물과 그 혼합물의 구강 내 병원균에 대한 항균효과 및 상호작용효과를 검색하였다. 알로에 ethanol 추출물 (AE)의 구강 병원균에 대한 최소저해농도는 S. mutans, C. albicans, E. coli에 대해서는 250$\mu\textrm{g}$/mL, E. fae-calis는 270$\mu\textrm{g}$/mL로 나타났다. 프로폴리스 ethanol 추출물(PE)의 경우에는 S. mutans에 대해서는 20 $\mu\textrm{g}$/mL, E. hirae, C. albicans, E. coli에 대해서는 20$\mu\textrm{g}$/mL, E. faecalis에 대해서는 40$\mu\textrm{g}$/mL로 나타났다. 수지 및 밀납성분을 제거한 분획(PW)의 S. mutans, E. hirae, C. albicans, E. coli의 최소저해농도는 70$\mu\textrm{g}$/mL로, PE 분획보다 낮은 항균활성을 보였으며, E.faecalis의 경우에는 18$\mu\textrm{g}$/mL로 PE분획보다 높은 항균활성을 보였다. AE 및 PE의 혼합물의 항균효과를 측정한 결과, S. mutans, E. faecalis, E. hirae, C. albicans에 대하여는 상승효과 (FICI=0.375)가 있었으며, AE 및 PE의 각각의 MIC의 1/2 이하의 모든 농도범위에서 상승효과(FICI$\leq$0.5)가 있었다. 그러나 E. coli에 대하여는 AE 및 PE의 혼합에 의한 상승효과가 없었다(FICI=1.0). 또한, AE 및 PW의 혼합물의 항균효과를 측정한 결과, S. mutans, E. faecalis, E. hirae, C. albicans에 대하여는 상승효과가 적은 것 (FICI=0.75)으로 나타났다. 즉, 사용된 구강 병원균주 중에서 E. coli를 제외한 모든 균주들에 대하여 PE 및 PW는 AE의 항균효과를 강화시키는 것으로 나타났다.

• 대한본초학회지
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• 제30권6호
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• pp.55-61
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• 2015

Objectives : Yongdamgosam-hwan(YGH) has been used as a traditional medicine from old times for antiinflammatory effects. Streptococcus mutans(S. mutans) is known as a prime bacteria responsible for causing caries by forming a biofilm referred to as dental plaque on the tooth surface. But antimicrobial activity of YGH with dental disease is not sufficiently understood. This study was designed to investigate the effects of YGH ethanol extract on antimicrobial effect against Streptococcus mutans.Methods : The antimicrobial effect of YGH ethanol extract was assessed by the paper disk diffusion method and optical density method to determine minimum inhibition concentration(MIC), also observed by fractional inhibitory concentration index(FICI) and time-kill assay to figure out the synergic effect on the combination of YGH ethanol extract with antibiotics.Results : The YGH ethanol extract 500 μg was 7.5-8.5 mm diameter of clear zone of inhibition against Streptococcus mutans in a concentration-dependent manner and MIC was 250 μg/mL. The administration of the ethanol extract in combination with gentamicin and streptomycin induced a reduction of ≥4-8-fold in all tested bacteria. Furthermore, time-kill study was found that a combination of YGH ethanol extract with oxacillin and streptomycin produced a more rapid decrease in the concentration of bacteria CFU/mL than the YGH ethanol extract or antibiotics alone.Conclusions : As a result, the YGH ethanol extract has good antimicrobial effects. And the results suggest that YGH could be employed as a natural antibacterial agent in dental care products.

• 대한수의학회지
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• 제42권1호
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• pp.45-54
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• 2002

The study was carried out to characterize the pharmacokinetics after intravenous (iv, 20 mg/kg) and oral (p.o. 100 mg/kg) administration as oxytetracycline (OTC) and tiamulin (TIA) mixture in swine and to determine interaction between OTC and TIA against various pig pathogenic bacteria. The antibacterial effects of OTC in combination with TIA in vitro showed synergistic effect against Salmonella typhimurium 1925, Pasteurella multocida Type A, P. multocida Type D, Krebsiella Pneumoniae 2001, K. Pneumoniae 1560, K. Pneumoniae 2208, Haemophillus pleuropneumonia S 2, and H. pleuropneumonia S 5, but against additive effect E. coli K88ab and S. choleraesuis on the basis of fractional inhibitory concentration (FIC) index. On the while, after i.v. and p.o. administration of OTC and TIA mixture, each OTC and TIA concentrations in plasma were fitted to an open two-compartment model. After i.v. administration of OTC-TIA mixture, the mean distribution half-life ($T_{1/2{\alpha}}$) of OTC and TIA in plasma showed 0.29 h and 0.17 h, and the mean elimination half-life ($T_{1/2{\beta}}$) of those was 4.36 h and 6.64 h, respectively. The mean volume of distribution at steady state ($Vd_{ss}$) of OTC and TIA was $0.85{\ell}/kg$ and $2.44{\ell}/kg$, respectively. After oral administration of OTC and TIA mixture, the mean maximal absorption concentrations ($C_{max}$) of OTC and TIA were $0.60{\mu}g/m{\ell}$ at 1.07 h ($T_{max}$) and $1.68{\mu}g/m{\ell}$ at 1.85 h ($T_{max}$), respectively. The mean elimination half-life ($T_{1/2{\beta}}$) of those showed 6.84 h and 6.36 h. In conclusion, we could suggest in this study that the combination of OTC and TIA may be recommended for the antibacterial therapy against polymicrobial infections, and both OTC and TIA showed large distribution to tissues and high $C_{max}$ after p.o. administration.