• Korean Journal of Agricultural Science
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• v.49 no.4
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• pp.795-807
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• 2022

The development and commercialization of industrial genetically modified (GM) organisms is actively progressing worldwide, highlighting an increased need for improved safety management protocols. We sought to establish an environmental monitoring method, using real-time polymerase chain reaction (PCR) and propidium monoazide (PMA) treatment to develop a quantitative detection protocol for living GM microorganisms. We developed a duplex TaqMan quantitative PCR (qPCR) assay to simultaneously detect the selectable antibiotic gene, ampicillin (AmpR), and the single-copy Escherichia coli taxon-specific gene, D-1-deoxyxylulose 5-phosphate synthase (dxs), using a direct cell suspension culture. We identified viable engineered E. coli cells by performing qPCR on PMA-treated cells. The theoretical cell density (true copy numbers) calculated from mean quantification cycle (Cq) values of PMA-qPCR showed a bias of 7.71% from the colony-forming unit (CFU), which was within ±25% of the acceptance criteria of the European Network of GMO Laboratories (ENGL). PMA-qPCR to detect AmpR and dxs was highly sensitive and was able to detect target genes from a 10,000-fold (10^-4) diluted cell suspension, with a limit of detection at 95% confidence (LOD_95%) of 134 viable E. coli cells. Compared to DNA-based qPCR methods, the cell suspension direct PMA-qPCR analysis provides reliable results and is a quick and accurate method to monitor living GM E. coli cells that can potentially be released into the environment.

• Journal of the Korean Society for Precision Engineering
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• v.10 no.3
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• pp.198-204
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• 1993

The study is concerned with finite element analysis and design of axisymmetric deep drawing by local heating. When the bottom shape of a cup is not flat but in complex-shaped, i.e., hemispherical, the cup cannot be drawn in one or two processes in the conventional deep drawing process and the limit drawing ratio is limited as well. By introducing local heating selectively with regards to the heating position, the formability of the sheet metal can be greatly increased with the reduced number of processes. In the Process analysisthe rigid- viscoplastic finite element method is employed and the temperature effect is incorporated. Bishop's step-wise decoupled method is employed to analyze the thermomechanical interaction between deformation and heat transfer. Axisymmetric deep drawing of a hemisphere-bottomed cup has been analyzed for various combinations of heat application in the punch and the die. At the first stage of deep drawing stretch forming is practically carried out by firmly pressing the blankholder with the punch and the die heated at various levels of temperature. Then at the second stage the same cup is drawn for the saame or different combination of temperature. From the computation, it has thus been shown that the fromability of a cup is greatly increased in two-stage deep drawing with increased limet drawing ratio.

• Microbiology and Biotechnology Letters
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• v.29 no.4
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• pp.212-220
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• 2001

Rare mating was used to select a respiratory deficient mutant of Saccharomyces cerevisiae HDC52 strain. Glucoamylase gene of S. diastaticus K114 was developed into the RD mutant which could uptake maximum amount of non-fermentable sugars through the expression of glu- coamplyase gene and the fermentation characteristics of the developed strain HCS were investigated. The size of HCS yeast and HBD52 yeast strain were 13 $\mu\textrm{m}$ and 10$\mu\textrm{m}$ respectively. HCS strain which can uptake maximum amount of non-fermentable sugar through the expression of glucoamylase gene was developed. By karyotype anal- ysis. HCS stain but not RD mutant HBC52 showed a band of 1150 kb chromosome DNA This band should include glcoamylase gene from Saccharomyces diataticus K114 THis strain has glucoamylase which can degrade starch By transduction and contrnuance of glucoamylase gene HCS strain gegraded strach and formed halo. Also, HCS strain maintained the character after 50 generations. Glucoamylase activities of Saccharomyces diastaticus K114 and HCS yeast strains are 9.5 and 2.7~3.4(unit/ml) HCS and HBC52 strain showed similar sugar fermentation patterns and low flocculation In spore and film forming test, HCS and HBC52 strain formed neither spores nor films. In the limit fermentation test, HBC52 strain showed fermentation level of 68% and HCS strain showed 76~78% As the limit attenuation of HBC52 and HCS were ($2.00^{\circ}$P) and ($0.7~0.93^{\circ}$P) This study demon- strates and HCS strain may be used for low carbohydrate beer fermentation.

• Proceedings of the KSR Conference
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• 2011.10a
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• pp.3268-3277
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• 2011

Uiwang ICD, which is the only inland container depot in the capital area, is equipped with modern facilities, and loading and unloading equipment, and can handle 1,000,000 TEU annually.It has many advantages such as advantageous conditions of location, the role of foothold for distribution transportation in the capital area, so it plays an important role of vitalizing the railroad transport. However, Uiwang ICD has reached the limit in the vitalization of railroad transport of containerized cargo due to the current problems in facilities and operations. This research analyzed the Uiwang ICD's problems by dividing them into facilities problems and operational problems, and presented short-term and medium and long-term improvement measures with the year 2023 when the private use permission expires as the basic year. However, the vitalization of railroad transport of Uiwang ICD can be attained only when it has support and cooperation from the government, Uiwang-si, Korea Rail Network Authority, and other related organizations together with efforts of Korea Railroad Corporation, and occupant companies. Such improvement measures for Uiwang ICD are expected to contribute to establishing combined transport system and forming efficient railroad distribution infrastructures and vitalizing railroad distribution, and also contribute to the government's low-carbon green growth policy by building environment-friendly transport system.

• Journal of Microbiology and Biotechnology
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• v.11 no.1
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• pp.21-28
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• 2001

Enzyme linked-immunosorbent assay (ELISA) for a rapid detection of fungi, Aspergillus and Penicillium genera in food, were developed and their efficiencies were approved by detecting artificially contaminated agricultural commodities. Mice were immunized with partially purified Aspergillus flavus extracellualr polysaccharide (EPS) and lymph node cells of the mice were fused with the myeloma cells for production of monoclonal antibodies. Mab 1G11, one of the antibodies, was selected and purified. A sandwich ELISA was established and its detection limit toward A. flavus EPS was 1mg/ml. Among the 59 strains tested (including 18 species of Aspergillus, 16 of Penicillium, 11 of Fusarium, 1 of Absidia, 2 of Alternaria, 2 of Candida, 2 of Cladosporium, 2 of Geotrichum, 2 of Mucor, 2 of Rhizopus, 1 of Trichoderma), species of Aspergillus and penicillium had a high reactivity with Mab 1G11 even up to 10,000 times dilution of culture broths. The other genera except Cladosporium resinae showed no reactivity, thus Mab 1G11 was specific to the genera of Aspergillus and Penicillium. The epitope of A. flavus EPS against monoclonal Mab 1G11 was on the carbohydrate moiety when 1 to 100$\mu g/g$ A. flavus EPS were put into rice, potato, and mandarin orange, the average recoveries detected by sandwich ELIA were 123, 59, and 76%, respectively. Correlation was found to be linear between the EPS, and mycelium of A. flavus and Penicillium citrinum grown in a liquid medium (r=0.87 and 0.96), and also between the EPS and colony forming unit in solid media of rice of potato (r=0.91-0.99).

• Transactions of Materials Processing
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• v.16 no.7
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• pp.509-515
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• 2007

As the springback of sheet metal during unloading may cause deviation from a desired shape, accurate prediction of springback is essential for the design of sheet stamping operations. When considering the case of a sheet metal being bent to radius $\rho$ that is such that the maximum stress induced exceed the elastic limit of the material, plastic strain in the outer surface will occur and the material will take a permanent set: but since, on removing the bending moment, the recovery of the material is not uniform across the thickness, springback will occur and the radius $\rho$ will not be maintained. Furthermore, when a tensile load being applied to each end of specimen, the tensile stress due to bending is increased and the compressive stress is decreased or cancelled and eventually the whole specimen may be in varying degree of tension. On the removal of the applied load the specimen loses its elastic strain by contracting around the contour of the block, the radius $\rho$ will be determined by the residual differential strain. Therefore in this study the springback is analytically estimated by the residual differential strains between upper and lower surfaces of greatest radius after elastic recovery, and a springback model based on the bending moment is also analytically derived for comparison purpose.

• Journal of Environmental Health Sciences
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• v.22 no.2
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• pp.43-57
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• 1996

To characterize worker's exposure to glass fibers, to find the correlation between airborne total dust concentrations and fiber concentrations and to recommend an appropriate evaluation method for worker's exposure to fibrous dusts in glass wool industry, we carried out this study. Average respirable fiber levels at five factories were 0.013-0.056 f/cc, and fairly below the OSHA PEL, 1 f/cc. A factory showed the lowest airborne fiber level, 0.013 f/cc, which was different significantly from those of other factories of which average fiber concentration was 0.046 f/cc. The cutting and grinding operations of insulation products resulted in higher airborne fiber cocentrations than any other processes(p<0.05). To characterize airborne fiber dimension, fiber length and diamter were determined using phase contrast microscope. The geometric means of airborne fiber lengths were $42-105 \mu m$. One factory had airborne fibers whose length distribution(GM = $105 \mu m$) was different from those of other factories(GM = $42-50 \mu m$). The percentages of respirable fibers less thinner than 3 gm were 38.9-90.9% at four factories, and two factories of them had the higher percentages than others. The findings explain for variation of airborne fiber diameters between factories. On the other hand, between the processes were the difference of fiber-length distributions observed. The cutting and grinding operations showed shorter fiber-length distributions than the fiber forming one. However, fiber-diameter distributions or respirable fiber contents were similar in all processes. The airborne fiber concentrations and the dust concentrations had relatively weak correlation(r=0.25), thus number of fibers couldn't be expected reliably from dust amount. Fiber count is appropriate for assessing accurate exposures and health effects caused by fibrous dusts including glass fibers. Ministry of Labor have established occupational exposure limit to glass fibers as nuisiance dust, but should establish it on the basis of respirable fiber concentration to provide adequate protection for worker's health

• ALGAE
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• v.19 no.4
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• pp.339-347
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• 2004

Seaweed community of the sub tidal rocky habitats along the coast of Geumo Archipelago in the central South Sea of Korea is described. This area is characterized by archipelago in which islets are separated by shallow bottom sediments (primary, muddy sand), and turbidity is generally high due to the resuspension of bottom sediments. The hard substrata available for algal attachment are limited to less than 10 m in depth. Thirty sites were randomly chosen along the coast from August 2003 to September 2003 and a 50 m long transect was established at each site. The transect began at a depth of 1 m and ended at the depth of 9 m. The percent cover of all species other than crustose coralline algae was estimated at 2 m depth intervals along the transect using a 0.25 m$^2$ PVC quadrat with 25 squares. Thirty-six species were identified including 6 Chlorophyta, 10 Phaeophyta and 20 Rhodophyta. Species with more than 5% mean bottom cover were Gelidium amansii, Corallina pilulifera, Amphiroa dilatata and Carpopeltis cornea, which formed dense turf-forming algal assemblages at 1-5 m depth. At all sites except S11-S15 located in the western coast of Sorido, bottom covers of seaweed species at the depth deeper than 7 m were less than 6%. The lower limit of algal assemblages was 9 m in depth. We speculate that the limited water clarity and vertical extent of hard substrata available for the settlement of seaweed species are the direct cause of reducing the diversity, abundance and distributional extent of algal assemblages in the area.

• Journal of Dairy Science and Biotechnology
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• v.33 no.4
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• pp.271-275
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• 2015

Colloidal semiconductor CdSe-ZnS core-shell nanocrystal quantum dot (Qdot) are luminescent inorganic fluorophores that show potential to overcome some of the functional limitations encountered with organic dyes in fluorescence labeling applications. Salmonella Enteritidis has emerged as a major cause of human salmonellosis worldwide since the 1980s. A rapid, specific, and sensitive method for the detection of Salmonella Enteritidis was developed using Qdot as a fluorescence marker coupled with immunomagnetic separation. Magnetic beads coated with anti-Salmonella Enteritidis antibodies were employed to selectively capture the target bacteria, and biotin-conjugated anti-Salmonella antibodies were added to form sandwich immune complexes. After magnetic separation, the immune complexes were labeled with Qdot via biotin-streptavidin conjugation, and fluorescence measurement was carried out using a fluorescence measurement system. The detection limit of the Qdot method was a Salmonella Enteritidis concentration of $10^3$ colony-forming units (CFU)/mL, whereas the conventional fluorescein isothiocyanate-based method required over $10^5CFU/mL$. The total detection time was within 2 h. In addition to the potential for general nanotechnology development, these results suggest a new rapid detection method of various pathogenic bacteria from a complex food matrix.

• Journal of Food Hygiene and Safety
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• v.22 no.4
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• pp.248-256
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• 2007

In this study, the competitive polymerase chain reaction (cPCR) was used to develop a direct enumeration method of Salmonella spp. in pork meat. After comparing three DNA extraction methods, the modified guanidine thiocyanate-phenol-chloroform method was chosen for Salmonella DNA extraction in artificially inoculated pork meat. The previously reported 284-bp invA gene (Rahn et al. Mol. Cell. Probes 1992) was tested for specificity, and 57 Salmonella strains and 24 non-Salmonella strains were evaluated. All Salmonella strains tested were invA positive, and all non-Salmonella strains produced no false positive amplification products. The detection limit achieved was as low as 1,460 colony-forming units (cfu) per 0.1g of pork meat. For cPCR, the invA gene, which features a 82 bp-deletion, was cloned in the pGEM-4Z vector. A known amount of competitor DNA, which has the same primer binding sites, was co-amplified with Salmonella chromosomal DNA from the artificially inoculated pork meat. The cell-number determined by cPCR was approximately equal to the cfu from the most probable number (MPN) method. Finally, the whole procedure took only 5 hr.