• 제목/요약/키워드: Forming Length

검색결과 354건 처리시간 0.024초

AFM을 이용한 스트렙타비딘-바이오틴 단백질 복합체의 흡착 분석 (Absorption analysis of streptavidin-biotin complexes using AFM)

  • 박지은;김동선;최호진;신장규;김판겸;임근배
    • 센서학회지
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    • 제15권4호
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    • pp.237-244
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    • 2006
  • Atomic force microscope (AFM) has become a common tool for the structural and physical studies of biological macromolecules, mainly because it provides the ability to perform experiments with samples in a buffer solution. In this study, structure of proteins and nucleic acids has been studied in their physiological environment that allows native intermolecular complexes to be formed. Cr and Au were deposited on p-Si (100) substrate by thermal evaporation method in sequence with the thickness of $200{\AA}$ and $500{\AA}$, respectively, since Au is adequate for immobilizing biomolecules by forming a self-assembled monolayer (SAM) with semiconductor-based biosensors. The SAM, streptavidin and biotin interacted each other with their specific binding energy and their adsorption was analyzed using the Bio-AFM both in a solution and under air environment. A silicon nitride tip was used as a contact tip of Bio-AFM measurement in a solution and an antimony doped silicon tip as a tapping tip under air environment. Actual morphology could also be obtained by 3-dimensional AFM images. The length and agglomerate size of biomolecules was measured in stages. Furthermore, $R_{a}$ (average of surface roughness) and $R_{ms}$ (mean square of surface roughness) and surface density for the adsorbed surface were also calculated from the AFM image.

다공막 주형에 의한 전도성 고분자 나노와이어의 합성 (Synthesis of Conductive Polymer Nano-wires by Porous Membrane Template)

  • 신화섭;염경호
    • 멤브레인
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    • 제22권1호
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    • pp.35-45
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    • 2012
  • 양극산화 알루미나(AAO)막의 나노 사이즈 미세공(세공 크기 20 nm, 10 nm 및 200 nm)을 주형으로 사용하여 전도성 고분자인 폴리피롤, 폴리아닐린 중합체 및 폴리피롤/폴리아닐린 공중합체 나노와이어를 제조하였다. 미세공 주형 내에서 전도성 고분자의 성장은 세공의 벽면을 따라 튜브 형태로서 성장하였으며, 3시간 이후에는 내부가 완전히 채워진 나노와이어가 형성되었다. AAO 막을 수산화나트륨 용액으로 퍼리하여 세공 내에 형성된 전도성 고분자 나노와이어를 회수 하였으며. 회수된 나노와이어�l 직경과 길이는 주형 막의 세공 형상과 일치하였다. 통상의 용액 중합법으로 제조된 전도성 고분자 분말과 비교하여 주형 합성법으로 제조된 전도성 고분자 나노와이어는 결정성과 열적 안정성이 향상되었으며, 전기 저항은 4~60% 감소하였다.

Multiple shoot induction and plant regeneration from axillary buds of Magnolia 'Vulcan'

  • Kim, Tae-Dong;Kim, Ji-Ah;Lee, Na-Nyum;Choi, Chang-Ho
    • Journal of Plant Biotechnology
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    • 제47권1호
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    • pp.40-45
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    • 2020
  • An efficient protocol for multiple shoot induction and plant regeneration from axillary bud culture of Magnolia 'Vulcan' was developed in the present study. Primary shoots were obtained from axillary bud explants cultured on Murashige and Skoog (MS) medium containing 1.0 mg/L 6-benzylaminopurine (BA). To induce multiple shoots effectively, primary shoot tips were cultured on MS medium supplemented with different concentrations of BA and zeatin at 0, 0.2, 0.5, and 1.0 mg/L. Of these treatments, the MS medium with 0.5 mg/L BA resulted in the highest number of shoots per explant with an average value of 5.9, and it produced the greatest shoot height at 4.8 cm after 12 weeks of culturing. In the rooting of in vitro produced shoots, the greatest percentage of explants forming roots (91.3%), number of roots per explant (9.7), and root length (2.8 cm) were obtained in half-strength MS medium supplemented with 6.0 mg/L indole-3-butyric acid (IBA). Regenerated plantlets were successfully acclimatized and hardened off inside the culture room with 87.5% survival rate. Plants were transferred to a greenhouse with a 97.2% survival rate. The highly efficient shoot multiplication and plant regeneration system reported herein can be used for large-scale clonal propagation of valuable Magnolia species or cultivars.

탄소섬유용 리오셀 전구체의 결정구조에 관한 연구 (Study of the Crystal Structure of a Lyocell Precursor for Carbon Fibers)

  • 박길영;김우성;이수오;황태경;김연철;서상규;정용식
    • 한국추진공학회지
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    • 제23권5호
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    • pp.36-42
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    • 2019
  • 본 연구에서는 리오셀 섬유를 사용하여 탄소직물을 제조함에 있어, 인계 난연제인 Phosphoric Acid(PA)와 가교제인 Melamine resin (MR)을 사용하여 섬유의 전처리를 수행하고 TGA, FT-IR, XRD, 중량 분석을 통하여 물리적, 화학적 구조 변화에 대하여 고찰하였다. 전처리를 통하여 내염화 및 흑연화된 직물의 경우 미처리 직물과 비교하여 중량 수율이 14.7%, 직물 폭과 길이의 수율이 각각 15%, 15.5% 증가함을 확인하였다. 이러한 결과는 셀룰로오스의 탈수반응을 촉진과 함께 섬유 표면에 char를 형성하고, 셀룰로오스 분자 내의 가교반응을 유도하여 내염화 시 안정한 구조 형성에 의한 효과로 설명할 수 있다.

Free vibrations of a two-cable network inter-supported by cross-links extended to ground

  • Zhou, H.J.;Wu, Y.H.;Li, L.X.;Sun, L.M.;Xing, F.
    • Smart Structures and Systems
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    • 제23권6호
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    • pp.653-667
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    • 2019
  • Using cross-ties to connect cables together when forming a cable network is regarded as an efficient method of mitigating cable vibrations. Cross-ties have been extended and fixed on bridge decks or towers in some engineering applications. However, the dynamics of this kind of system need to be further studied, and the effects of extending cross-links to bridge decks/towers on the modal response of the system should be assessed in detail. In this paper, a system of two cables connected by an inter-supported cross-link with another lower cross-link extended to the ground is proposed and analyzed. The characteristic equation of the system is derived, and some limiting solutions in closed form of the system are derived. Roots of cable system with special configurations are also discussed, attention being given to the case when the two cables are identical. A predictable mode behavior was found when the stiffness of inter-connection cross-link and the cross-link extended to the ground were the same. The vector of mode energy distribution and the degree of mode localization index are proposed so as to distinguish global and local modes. The change of mode behaviors is further discussed in the case when the two cables are not identical. Effects of cross-link stiffness, cross-link location, mass-tension ratio, cable length ratio and frequency ratio on $1^{st}$ mode frequency and mode shape are addressed.

부채꼴 영역 기반의 동적인 충돌 영역을 이용한 입자 기반 충돌 검사의 고속화 기법 (Acceleration Technique in Particle-based Collision Detection Using Cone Area Based Dynamic Collision Regions)

  • 김종현
    • 한국컴퓨터그래픽스학회논문지
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    • 제25권2호
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    • pp.11-18
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    • 2019
  • 본 논문에서는 많은 개체와의 충돌 검사를 요구하는 입자 기반 시스템에서 부채꼴 영역의 동적인 변화를 이용하여 효율적으로 충돌 검사를 가속화시킬 수 있는 프레임워크를 제안한다. 입자와 부채꼴 기반의 충돌 영역은 다음 세 가지 조건에 의해 결정된다: 1) 인접 입자의 반경 내에 부채꼴의 위치가 존재하는 경우, 2) 부채꼴 영역 내에 인접 입자의 위치가 존재하는 경우, 3) 부채꼴 영역을 형성하는 두 벡터 사이에 인접 입자가 존재하는 경우. 결과적으로 위 조건들을 모두 만족했을 때 입자와 부채꼴 영역은 충돌되었다고 정의한다. 본 논문에서는 입자의 움직임에 따라 충돌 검사 범위인 부채꼴의 영역을 자동으로 업데이트 한다. 부채꼴 영역의 동적인 변화를 계산하기 위해 입자의 위치와 속도를 기반으로 부채꼴의 방향, 길이, 각도를 조절한다. 최종적으로 계산된 부채꼴 영역 내에 있는 입자들만을 이용하여 충돌 검사를 빠르게 수행한다. 본 연구에서 제안하는 가속화 방법은 트리와 같은 자료구조를 명시적으로 만들지 않고, 닫힌 형태 방정식으로 실행되기 때문에 간단하게 구현되며 모든 결과에서 충돌 검사 성능이 개선되었다.

한복과 한푸의 차이점 분석에 관한 연구 (A Study on the Difference between the Roots of (K)Hanbok and Hanfu)

  • 김지수;나영주
    • Human Ecology Research
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    • 제60권2호
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    • pp.273-287
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    • 2022
  • (K)Hanbok, which is Korea's traditional clothing, differs from the Chinese Hanfu or Japanese Kimono. This study aims to understand the fundamental differences between (K)Hanbok and Hanfu. The Goryeo Dynasty (K)Hanbok, which was particularly popular in China, was established because the Ming Dynasty Hanfu and Chinese fashion were considerably influenced by the 'Koryo Yang'. Firstly, while (K)Hanbok is bulky, Hanfu of the Han Dynasty is characterized by forming a slim silhouette. Due to the climate of the Northern Hemisphere, (K)Hanbok shows a rich silhouette comprising multiple layers of inner pants and a pleated skirt over a voluminous underskirt. On the other hand, the Han's Hanfu creates a straight silhouette in the form of a wrap, revealing the contours of the body. The pleated skirt of the (K)Hanbok can use six to twelve width fabrics, depending on the social position; however, the Hanfu of the Han is a skirt without any pleats. Secondly, the clothing patterns, which have various shapes, are totally different in how they are made and sewn. The Korean (K)Hanbok is a two-piece separate, whereas the Chinese Hanfu style is a one-piece with a skirt. The short length of the (K)Hanbok jacket has a Sup which is cut and pasted allowing the front closure to overlap. Nevertheless, the Hanfu of the Han does not have this Sup because it is of a wrap-around, one-piece style and has an exceptionally large front, and wraps around at the waist which extends to the sides. Thirdly, the (K)Hanbok jacket has separate string Gorums for fastening, and an additional belt around the waist; however, in the case of Gorum, it is unnecessary for a wrapping style of Hanfu. Fourthly, Koreans as an agricultural horse-riding people, basically wore the trousers attached a comfortable gusset, while the Chinese Hanfu had no pants, but the Chinese wore Gaedanggo pants which exposed the hips, inevitably during the Warring States period.

Phylogenetic placement of thermophilic ammonium-tolerant bacteria and their distribution in various composts

  • Kazutaka Kuroda
    • Animal Bioscience
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    • 제36권4호
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    • pp.671-678
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    • 2023
  • Objective: Previous studies isolated the thermophilic ammonium-tolerant (TAT) bacterium Bacillus sp. TAT105 that grew in composting swine manure with the assimilation of ammonium nitrogen and reduced ammonia emissions during composting. Those studies also investigated the potential for applications of TAT105 to composting. It was observed that the concentration of TAT bacteria, phylogenetically close to TAT105, increased during composting. The objectives of this study were to identify the phylogenetic placement of these TAT bacteria and investigate their distribution in various composts. Methods: The phylogenetic placement of TAT105 was examined based on the sequence of 16S ribosomal RNA gene. The genomic DNA homology between TAT105 and the type strains of bacterial species that were phylogenetically close to TAT105 were examined by DNA-DNA hybridization. Moreover, the tolerances of these strains to NH4Cl and NaCl were analyzed using a cultivation method. Concentrations of TAT bacteria in various composts were evaluated using an agar medium specific to TAT bacteria and polymerase chain reaction followed by restriction fragment length polymorphism analysis. Results: TAT105 was most closely related to Bacillus thermolactis and Bacillus kokeshiiformis. Many variants of these species have been detected in various environments, including composts. The type strains of these species displayed TAT characteristics that were similar to those of TAT105. Among the composts examined in this study, TAT bacteria were detected at high concentrations (105 to 109 colony forming units per gram of dry matter) in most of the composts made from cattle manure, swine manure, bark, and excess sludge. Conclusion: TAT bacteria comprised B. thermolactis, B. kokeshiiformis, and their phylogenetically close relatives. They were considered to be adaptable to composting of some certain materials, and a favorable target for searching for strains with some useful function that could be applied to composting of these materials.

블루 진의 시각적 이미지에 관한 연구 - 한국과 중국 20대 여성을 중심으로 - (Research on the Visual Image of Blue Jeans - Focusing on Korean and Chinese Women in Their 20s -)

  • 덩한쉬;김정미
    • 한국의상디자인학회지
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    • 제26권1호
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    • pp.1-16
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    • 2024
  • This study aims to investigate the differences in blue jean styles and the visual images associated with them. To achieve this, literature reviews, case studies, and surveys were conducted. The results are as follows. 1) Based on previous studies, it was found that the design elements influencing the style of blue jeans and their visual images include waistline position, silhouette, details, decorations, and color. Additionally, differences in the preferences and visual evaluations of Korean and Chinese individuals regarding the style of blue jeans were confirmed. 2) Skinny blue jeans are typically characterized by a high waistline and a length that reaches the ankles, featuring a snug fit with stretchy fabric. Straight blue jeans, on the other hand, usually have a natural or high waistline and cover the instep, with a straight silhouette descending from the thigh to the hem. Bell-bottom blue jeans are primarily high-waisted and cover the instep with a silhouette forming a bell shape from the knee to the hem. 3) Korean and Chinese women in their 20s evaluated skinny, straight, and bell-bottom styles of blue jeans as having a 'casual' image. However, they also associated bell-bottom style blue jeans with 'unique' and 'attractive' images. Interestingly, Korean women described skinny-style blue jeans as making someone look 'youthful', while Chinese women perceived them as 'sophisticated', 'making the waist look thicker', and 'unassuming'. As for straight-style blue jeans, Korean women saw them as 'well-fitting', while Chinese women described them as 'making one appear taller', 'desirable', and 'free-spirited'.

미세균열이 합천지역 쥬라기 화강암의 압열인장강도에 미치는 영향 (Influence of Microcrack on Brazilian Tensile Strength of Jurassic Granite in Hapcheon)

  • 박덕원;김경수
    • 광물과 암석
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    • 제34권1호
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    • pp.41-56
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    • 2021
  • ① 미세균열의 길이(N=230), ② 미세균열의 간격(N=150) 및 ③ 압열인장강도(N=30)를 이용하여 쥬라기의 합천화강암에서 발달된 여섯 결(R1~H2)의 특성을 분석하였다. 여섯 결에 평행한 방향으로 측정한 이들 세 인자에 대한 18개의 누적그래프를 상호 대비하였다. 분석한 주요 결과를 요약하면 다음과 같다. 첫째, 9개 계급구간으로 구분한 압열인장강도값(kg/㎠)의 분포율(%)은 60~70(3.3) < 140~150(6.7) < 100~110·110~120(10.0) < 90~100(13.3) < 80~90(16.7) < 120~130·130~140(20.0)의 순으로 증가한다. 각 계급구간의 빈도수에 따른 강도의 분포곡선은 이봉 분포를 보여 준다. 둘째, 길이, 간격 및 인장강도에 대한 그래프를 H2 < H1 < G2 < G1 < R2 < R1의 순으로 배열하였다. 간격과 길이에 대한 두 그래프 사이의 지수차(λS-λL, Δλ)는 H2(-1.59) < H1(-0.02) < G2(0.25) < G1(0.63) < R2(1.59) < R1(1.96)(2 < 1)의 순으로 증가한다. 관련 도면으로부터, 상기한 지수차의 증가와 함께 인장강도에 대한 여섯 그래프는 점차 좌측 방향으로 이동한다. 조직의 균일도를 지시하는 인장강도에 대한 그래프의 음의 기울기(a)는 3번 결((H1+H2)/2, 0.116) < 2번 결((G1+G2)/2, 0.125) < 1번 결((R1+R2)/2, 0.191)의 순으로 증가한다. 셋째, 각 결(R1·R2(1번 결), G1·G2(2번 결), H1·H2(3번 결))을 구성하는 두 방향에 대한 그래프 사이의 배열순을 비교하였다. 길이와 간격에 대한 두 그래프의 배열순은 상호 역순이다. 간격과 인장강도에 대한 두 그래프는 배열순에서 서로 일관성이 있다. 길이와 간격에 대한 지수차(ΔλL 및 ΔλS)는 1번 결(R, -0.08) < 2번 결(G, 0.14) < 3번 결(H, 0.75) 및 3번 결(H, 0.16) < 2번 결(G, 0.23) < 1번 결(R, 0.45)의 순으로 각각 증가한다. 넷째, 미세균열의 길이, 미세 균열의 간격 및 인장강도의 분포 특성을 보여 주는 여섯 그래프에 대한 종합도를 작성하였다. 길이의 범위에 따라, 여섯 그래프는 G2 < H2 < H1 < R2 < G1 < R1(<7 mm) 및 G2 < H1 < H2 < R2 < G1 < R1(≦2.38 mm)의 순을 보여 준다. 간격에 대한 여섯 그래프는 누적 빈도수 12 및 간격 0.53 mm에 해당하는 지점 부근에서 병목구간을 형성하여 서로 교차한다. 다섯째, 여섯 결을 대변하는 각 파라미터의 여섯 값을 증가 및 감소하는 순으로 배열하였다. 길이와 관련된 8개 파라미터 중에서, 총 길이(Lt) 및 그래프(≦2.38 mm)는 배열순에서 상호 부합한다. 간격과 관련된 7개 파라미터 중에서, 간격의 빈도수(N), 평균 간격(Sm) 및 그래프(≦5 mm)는 배열순에서 상호 일관성이 있다. 배열순의 측면에서, 간격에 대한 상기 세 파라미터의 값은 그룹 E에 속하는 최대인장강도와 일관성이 있다. 표 8에서와 같이, 이들 파라미터 값의 배열순은 여섯 결 및 세채석면에 대한 사전 인식에 유용하다.