• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.17 no.12
• /
• pp.2853-2858
• /
• 2013

This paper proposes the ITMS(Intelligent Transport Monitoring System) which manages the route and state of freight by using the Meteorological Office, the Transportation Management Center, GPS and Sensors, etc. The ITMS consists of the CIMS(Container Inner Monitoring System) transmitting the inner temperature and humidity of a container, the TMM(Transport Management Module) computing an estimated time of arrival with Freight Vehicle location information and transmits the result to the CIMS, the FMM(Freight Management Module) checking and managing the freight freshness by using the temperature and humidity of the collected containers, and the SMM(Stevedoring Management Module) selecting the container loading and unloading places with the information transmitted from the CIMS, the TMM, and the FMM and attaching the freight formation to containers using an RFID label. The ITMS not only checks the freight condition at intervals but also acquires and manages the freight information with RFID labels rapidly and accurately.

• Molecules and Cells
• /
• v.27 no.2
• /
• pp.149-157
• /
• 2009

In both gram-positive and several gram-negative bacteria, the transcription of dnaK and groE operons is negatively regulated by HrcA; however, the mechanism modulating HrcA protein activity upon thermal stress remains elusive. Here, we demonstrate that HrcA is modulated via reduction and oligomerization in vitro. Native-PAGE analysis was used to reveal the oligomeric structure of HrcA. The oligomeric HrcA structure became monomeric following treatment with the reducing agent dithothreitol, and this process was reversed by treatment with hydrogen peroxide. Moreover, the mutant HrcA C118S exhibited reduced binding to CIRCE elements and became less oligomerized, suggesting that cysteine residue 118 is important for CIRCE element binding as well as oligomerization. Conversely, HrcA mutant C280S exhibited increased oligomerization. An HrcA double mutant (C118S, C280S) was monomeric and exhibited a level of oligomerization and CIRCE binding similar to wild type HrcA, suggesting that cysteine residues 118 and 280 may function as checks to one another during oligomer formation. Biochemical fractionation of E. coli cells overexpressing HrcA revealed the presence of HrcA in the membrane fraction. Together, these results suggest that the two HrcA cysteine residues at positions 118 and 280 function as reduction sensors in the membrane and mediate oligomerization upon stress.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.38 no.3
• /
• pp.276-283
• /
• 2011

Recently, undifferentiated stem cells which exist in dental papillae of immature permanent teeth were newly discovered and these stem cells appear to be the origin of ameloblasts associated with the formation of root dentin. When treating immature permanent teeth, the preservation of these stem cells induce the continuous formation of the root. Therefore, it is reported that minimal invasion to periapical region in immature permanent teeth with periapical inflammation resulted in good-healing pattern in clinical and radiographic examination. In this case, a 10 year-old boy(mandibular right premolar) and a 8 year-old girl(maxillary left premolar) who visited the department of pediatric dentistry at Chosun University Dental Hospital were diagnosed with pulp necrosis and periapical abscess in clinical and radiographic examination. Endodontic instrumentation to the periapical region was limited and MTA(Mineral Trioxide Aggregate) was applied into the pulp canal. The periodic checks showed healing of periapical abscess and the development and growth pattern of roots. In permanent teeth with pulp necrosis and periapical abscess, preservation of pulp and dental papillae in the periapical region showed good prognosis during the periodic examinations. Therefore, a lot of clinical examination and long-term evaluation of conservative pulp treatment in immature permanent teeth are expected to be necessary.