• Title/Summary/Keyword: Formation Parameters

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Adhesive Properties, Extracellular Protein Production, and Metabolism in the Lactobacillus rhamnosus GG Strain when Grown in the Presence of Mucin

  • Sanchez, Borja;Saad, Naima;Schmitter, Jean-Marie;Bressollier, Philippe;Urdaci, Maria C.
    • Journal of Microbiology and Biotechnology
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    • v.20 no.6
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    • pp.978-984
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    • 2010
  • This paper examines the probiotic bacterium Lactobacillus rhamnosus GG, and how it reacts to the presence of mucin in its extracellular milieu. Parameters studied included cell clustering, adhesion to mucin, extracellular protein production, and formation of final metabolites. L. rhamnosus GG was found to grow efficiently in the presence of glucose, N-acetylglucosamine, or mucin (partially purified or purified) as sole carbon sources. However, it was unable to grow using other mucin constituents, such as fucose or glucuronic acid. Mucin induced noticeable changes in all the parameters studied when compared with growth using glucose, including in the formation of cell clusters, which were easily disorganized with trypsin. Mucin increased adhesion of the bacterium, and modulated the production of extracellular proteins. SDS-PAGE revealed that mucin was not degraded during L. rhamnosus GG growth, suggesting that this bacterium is able to partially use the glucidic moiety of glycoprotein. This study goes some way towards developing an understanding of the metabolic and physiological changes that L. rhamnosus GG undergoes within the human gastrointestinal tract.

Protection against Whole Body γ-Irradiation Induced Oxidative Stress and Clastogenic Damage in Mice by Ginger Essential Oil

  • Jeena, Kottarapat;Liju, Vijayasteltar B;Ramanath, Viswanathan;Kuttan, Ramadasan
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.3
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    • pp.1325-1332
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    • 2016
  • Radioprotective effects of ginger essential oil (GEO) on mortality, body weight alteration, hematological parameters, antioxidant status and chromosomal damage were studied in irradiated mice. Regression analysis of survival data in mice exposed to radiation yielded LD50/30 as 7.12 and 10.14 Gy for control (irradiation alone) and experimental (GEO-treated irradiated) mice, respectively, with a dose reduction factor (DRF) of 1.42. In mice exposed to whole-body gamma-irradiation (6 Gy), GEO pre-treatment at 100 and 500 mg/kg b.wt (orally) significantly ameliorated decreased hematological and immunological parameters. Radiation induced reduction in intestinal tissue antioxidant enzyme levels such as superoxide dismutase, catalase, glutathione peroxidase and glutathione was also reversed following administration of GEO. Tissue architecture of small intestine which was damaged following irradiation was improved upon administration of GEO. Anticlastogenic effects of GEO were studied by micronuclei assay, chromosomal aberration and alkaline gel electrophoresis assay. GEO significantly decreased the formation of micronuclei, increased the P/N ratio, inhibited the formation of chromosomal aberrations and protected agaisnt cellular DNA damage in bone marrow cells as revealed by comet assay. These results are supportive of use of GEO as a potential radioprotective compound.

Effects of Weight-Bearing Exercise on Bone Metabolism in College Women (체중부하운동이 여대생의 골대사에 미치는 효과)

  • 김주성;김명희;신재신
    • Journal of Korean Academy of Nursing
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    • v.34 no.5
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    • pp.760-770
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    • 2004
  • Purpose: This study was done to identify the effects of weight-bearing exercise(WBE) on bone metabolism. Method: WBE was performed for 12 weeks by healthy college women. Bone-related parameters were measured four times during this period by evaluating the immunoradiometric assay and enzyme immunoassay. Bone mineral densities(BMDs) were measured by dual energy x-ray absorptiometry before and after the WBE program. Data was analyzed using t-test, paired t-test, $x^2$-test, and repeated measures ANOVA. Result: Osteocalcin, a bone formation marker, increased more in the experimental group than in the control group based on the interaction between time and group(F=3.29 p=.024). Little difference between the two groups was found for the other parameters: urinary deoxypyridinoline, insulin-like growth factorI, parathormone, serum calcium, and serum phosphorus without showing any time interaction between the groups. The femoral trochanter BMD rose in the experimental group while that of the control group fell, showing a significant difference for BMD(t=3.06 p=.005). However, there was no significant difference between the two groups for changes in BMD of the forearm, lumbar spine, femoral neck, and femoral ward's triangle. Conclusion: These findings supported the WBE is beneficial for increasing bone formation in college women and long-term application is needed to substantiate the effects of WBE as a intervention in promotion of bone-health.

EEC-FM: Energy Efficient Clustering based on Firefly and Midpoint Algorithms in Wireless Sensor Network

  • Daniel, Ravuri;Rao, Kuda Nageswara
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.12 no.8
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    • pp.3683-3703
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    • 2018
  • Wireless sensor networks (WSNs) consist of set of sensor nodes. These sensor nodes are deployed in unattended area which are able to sense, process and transmit data to the base station (BS). One of the primary issues of WSN is energy efficiency. In many existing clustering approaches, initial centroids of cluster heads (CHs) are chosen randomly and they form unbalanced clusters, results more energy consumption. In this paper, an energy efficient clustering protocol to prevent unbalanced clusters based on firefly and midpoint algorithms called EEC-FM has been proposed, where midpoint algorithm is used for initial centroid of CHs selection and firefly is used for cluster formation. Using residual energy and Euclidean distance as the parameters for appropriate cluster formation of the proposed approach produces balanced clusters to eventually balance the load of CHs and improve the network lifetime. Simulation result shows that the proposed method outperforms LEACH-B, BPK-means, Park's approach, Mk-means, and EECPK-means with respect to balancing of clusters, energy efficiency and network lifetime parameters. Simulation result also demonstrate that the proposed approach, EEC-FM protocol is 45% better than LEACH-B, 17.8% better than BPK-means protocol, 12.5% better than Park's approach, 9.1% better than Mk-means, and 5.8% better than EECPK-means protocol with respect to the parameter half energy consumption (HEC).

Evaluation of Glass-forming Ability in Ca-based Bulk Metallic Glass Systems (칼슘기 벌크 비정질 합금에서 비정질 형성능 평가)

  • Park, Eun-Soo;Kim, Do-Hyang
    • Journal of Korea Foundry Society
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    • v.29 no.4
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    • pp.181-186
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    • 2009
  • The interrelationship between new parameter ${\sigma}$ and maximum diameter $D_{max}$ is elaborated and discussed in comparison with four other glass forming ability (GFA) parameters, i.e. (1) super-cooled liquid region ${\Delta}T_x (=T_x - T_g)$, (2) reduced glass transition temperature $T_{rg} (=T_g/T_l)$, (3) K parameter $K (=[T_x-T_g]/[T_l -T_x])$, and (4) gamma parameter ${\gamma}(=[T_x]/[T_l+T_g])$ in Ca-based bulk metallic glass (BMG) systems. The ${\sigma}$ parameter, defined as ${\Delta}T^*{\times}P^'$, has a far better correlation with $D_{max}$ than the GFA parameters suggested so far, clearly indicating that the liquid phase stability and atomic size mismatch dominantly affect the GFA of Ca-based BMGs. Thus, it can be understood that the GFA of BMGs can be properly described by considering structural aspects for glass formation as well as thermodynamic and kinetic aspects for glass formation.

Laser Weldability of Sheet steels for Tailored Blank Manufacturing(1) (테일러드 블랭크용 박판 강재의 레이저 용접성 (1))

  • 김기철
    • Journal of Welding and Joining
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    • v.16 no.1
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    • pp.77-87
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    • 1998
  • This paper deals with the effect of laser welding parameters on the weld formation. Thin steels for automotive application were prepared so as to be welded with high power carbon dioxide laser system. Major process parameters were position of focus and travel speed. The effect of shielding gas was also discussed by employing the high speed photometry. Test results showed that the optimal position of focus varied in accordance with the joint configuration; bead-on-plate, butt or lap welding. It was recommended that the position of focus for the lap welding be located at slightly inner part of the material to be welded. In this case, however, it was noticeable that the weld penetration ratio, d/t$_{0}$ dropped drastically at the critical region. Results also demonstrated that both the bead width and penetration reduced as the travel speed increased. The penetration ratio showed two distinct regions; stabilized zone at the lower range of the travel spped and sudden drop zone at the higher range of travel speed. Lower limit of the penetration for acceptable weld was proved to be about 90% of the parent metal thickness based on the physical properties of the weld. Mixed gas application for both the shielding of molten metal and laser induced plasma control was recommended as far as the penetration was concerned.d.

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A Preliminary Study on the Lamination Characteristics of Inconel 718 Superalloy on S45C Structural Steel using LENS Process (LENS 공정을 이용한 Inconel 718 초합금의 S45C 구조용강 위 적층 특성 고찰에 관한 기초 연구)

  • Kim, Hyun-Sik;Lee, Hyub;Ahn, Dong-Gyu
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.20 no.1
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    • pp.16-24
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    • 2021
  • A laser-engineered net shaping (LENS) process is a representative directed energy deposition process. Deposition characteristics of the LENS process are greatly dependent on the process parameters. The present paper preliminarily investigates deposition characteristics of Inconel 718 superalloy on S45C structural steel using a LENS process. The influence of process parameters, including the laser power and powder feed rate, on the characteristics of the bead formation and the dilution in the vicinity of the deposited region is examined through repeated experiments. A processing map and feasible deposition conditions are estimated from viewpoints of the aspect ratio, defect formation, and the dilution rate of the deposited bead. Finally, an appropriate deposition condition considering side angle, deposition ratio, and buy-to-fly (BTF) is predicted.

Dissimilar Metal Welding of SM45C and STS304 by means of CW Nd:YAG Laser (CW Nd:YAG 레이저를 이용한 SM45C와 STS304의 이종금속용접)

  • 신호준;유영태;임기건;안동규
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2004.10a
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    • pp.1369-1375
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    • 2004
  • For many years and primarily for economical reasons, Dissimilar Metal Welds have been used as transition joints in a variety of equipment and applications. But Dissimilar Metal Welds have several fabrication and metallurgical drawbacks that can often lead to in-service failures. For example, the most pronounced fabrication faults are hot cracks. Laser welding techniques have been characterised for various materials. In this paper, the laser weldability of STS304 stainless steel and SM45C at dissimilar metal welds using a continuous wave Nd:YAG laser are experimentally investigated. An experimental study was conducted to determine effects of welding parameters, on eliminating or reducing the extent welding zone formation at dissimilar metal welds and to optimize those parameters that have the most influence parameters such as focus length, power, beam speed, shielding gas, and wave length of laser were tested

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On the Stochastic Stability Criteria for the Analysis and Simulation of Ocean Waves (수치실험조건에 따른 해양피낭특성의 통계적 안정한계)

  • RYU Cheong-Ro;KIM Hyeon-Ju
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.20 no.5
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    • pp.457-462
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    • 1987
  • Stochastic stability criterias for ocean wave analysis add simulation are studied using the data simulated by the linear superposition method. To clarify the criterias, the effects of the simulation parameters on the variance of stochastic properties of ocean waves are investigated, and the stable conditions of the parameters are estimated through the comparative study on the stochastic properties of simulated waves and well-known ocean waves. The simulation parameters considered are high frequency cut-off, data length, and number and phase angle of component waves. Statistical characteristics analysed are wave height, period and steepness, and the formation of groups of higher waves, resonance periods, steeper higher waves and extreme run-length of the run.

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Dissimilar Metal Welding of Austenite Stainless Steel and Low Carbon Steel using CW Nd:YAG Laser (CW Nd:YAG 레이저를 이용한 저 탄소강과 오스테나이트계 스테인레스강의 이종금속 용접)

  • Yoo Young Tae;Shin Ho Jun;Ahn Dong-Gyu;Im Kiegon
    • Journal of the Korean Society for Precision Engineering
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    • v.22 no.8 s.173
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    • pp.17-26
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    • 2005
  • Dissimilar-metal welds(DHWs) are used widely in various industrial applications due to the pratical importance from the technical and economic aspect. However, DMWs have several fabricative and metallurgical drawbacks that can often lead to in-service failures. For example, the most pronounced fabrication faults are hot cracks. Recently, DMWs have used the various of heat source to decrease such as faults. In this paper, the weldability on dissimilar metal welds of Austenite stainless steel and Low carbon steel using a continuous wave Nd:YAG laser was experimentally investigated. Experiments were conducted to determine effects of welding parameters, on eliminating or reducing the extent welding zone formation at dissimilar metal welds and to optimize those parameters that have the most influence parameters such as focus length, power, beam speed, shielding gas, and wave length of laser.