• Journal of Microbiology and Biotechnology
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• v.25 no.9
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• pp.1433-1441
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• 2015

Pollution resulting from the discharge of textile dyes into water systems has become a major global concern. Because peroxidases are known for their ability to decolorize and detoxify textile dyes, the peroxidase activity of Vitreoscilla hemoglobin (VHb) has recently been studied. It is found that VHb and variants of this enzyme show great promise for enzymatic decolorization of dyes and may play a role in achieving their successful removal from industrial wastewater. The level of VHb peroxidase activity correlates with two amino acid residues present within the conserved distal pocket, at positions 53 and 54. In this work, sitedirected mutagenesis of these residues was performed and resulted in improved VHb peroxidase activity. The double mutant, Q53H/P54C, shows the highest dye decolorization and removal efficiency, with 70% removal efficiency within 5 min. UV spectral studies of Q53H/P54C reveals a more compact structure and an altered porphyrin environment (λ_Soret = 413 nm) relative to that of wild-type VHb (λ_Soret = 406), and differential scanning calorimetry data indicate that the VHb variant protein structure is more stable. In addition, circular dichroism spectroscopic studies indicate that this variant's increased protein structural stability is due to an increase in helical structure, as deduced from the melting temperature, which is higher than 90℃. Therefore, the VHb variant Q53H/P54C shows promise as an excellent peroxidase, with excellent dye decolorization activity and a more stable structure than wild-type VHb under high-temperature conditions.

• Journal of Microbiology and Biotechnology
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• v.22 no.11
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• pp.1482-1485
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• 2012

A natural furan derivative was isolated from the methanolic extract of the fruit bodies of Fomitiporia ellipsoidea. Its chemical structure was elucidated as methyl 3,5-dioxo-1,3,5,7-tetrahydrobenzo[1,2-c:4,5-c']difuran-4-carboxylate by means of extensive NMR and MS data analysis, and named as fomitiporiaester A (1). Compound 1 showed significant antitumor activity to hepatoma $H_{22}$ in vivo, and the inhibition rates were 42.94%, 49.17%, and 58.15% at concentrations of 5, 10, and 20 mg/kg, respectively. Compound 1 showed weak cytotoxic activities against the human hepatoblastoma (HepG-2) and human oophoroma (Skov 3) cell lines with$IC_{50}$ values of more than $100{\mu}M$.

• Korean Journal of Ecology and Environment
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• v.53 no.1
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• pp.1-10
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• 2020

The Stream/River Ecosystem Survey and Health Assessment has been carried out regarding the ecological health of the streams by the Ministry of Environment (MOE), South Korea. However, the sources of impairment of the stream ecosystem and the interactions between the sources, stressors, and the responses of impaired streams have not been taken into consideration. The purpose of this study is to propose the establishment of diagnosis systems for the impaired stream ecosystem because of the need to incorporate the same in the making of the policy to enable the recovery and improvement of the health of the impaired streams or river ecosystem. First, we define the concept of a diagnosis of the impaired stream or river ecosystem through a literature review. Second, through case studies [e.g., US CADDIS (Causal Analysis/Diagnosis Decision Information System), AUS. Eco Evidence, EU WFD (Water Framework Directive)], we try to develop the diagnosis system for the making of policy. In this study, the diagnosis system that is proposed consists of eight steps including the basic data collection, detecting or suspecting impairment, defining the impaired stream reach, identifying the biological impaired cases and listing the candidate causes, illustrating the interactive conceptual diagrams between stressors and responses, investigating the stressors-responses in the field, verifying causes and identifying the probable causes of the impaired cases, and summarizing and proposing the restoration of the streams. The results of this study will support and enable efficient decision-making for sustainable stream restoration and management based on the diagnosis of the probable causes for the impaired complex and the diverse stream ecosystem.

• Korean Journal of Environment and Ecology
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• v.26 no.1
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• pp.100-112
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• 2012

Governments and related agencies have made efforts to introduce tree burials (TB) as an alternative to solve the problems involved in the traditional funeral manners. Citizens' TB selection rates are, however, still very low. It is important to understand citizens' perceptions and attitudes towards TB to make people more actively participate in TB. This study examined citizens' knowledge, behavioral belief, attitudes towards selecting TB, intentions to select TB, and their relationship with citizens' socio-demographic characteristics. A total of 320 citizens in Gwang-ju, Korea, completed the survey during May, 2011. Results showed that 65.3% of respondents were aware of TB, and many of them (47.6%) obtained information from TV/Radio. Age, education level, marital status and birth order were related with knowledge about TB. Respondents with higher age or higher education level tended to have higher knowledge rates. The married or the non-eldest sons also had higher knowledge rates. Respondents with higher age or higher education level, and the married tended to have more positive attitudes towards selecting TB and to rate 'unconventional funeral customs' high. Respondents with higher age, education level or married respondents tended to have higher intention to select TB. The knowledge level about TB was positively related with attitudes about TB selection and intention to select TB. The results were discussed and some ways to promote TB were suggested.

• The Korean Journal of Community Living Science
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• v.15 no.3
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• pp.29-43
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• 2004

The purpose of this study was to calculate the economic effect of Women-Farmer's Center. Since 2001, The Ministry of Agriculture and Forestry has run Women-Farmer's Centers in which women-farmers can receive the care for their pre-schooling children, after-school learning service, and city-farm exchange, education, and counseling. In other words, Women Farmer's Center provides not only improvement of ease and quality of life of women-farmer's, but also spreading economic effect to community and country. By calculation based on business plan of 14 centers that run centers, total economic income effects are 2,784 million won, which consist of 1,265 million won for counseling, 146 million won for the care of infants and children, 139 million won after-school learning, 1,020 million won for education, and 214 million won for city-farm exchange program. The Women-Farmer's Center should be managed reasonably with government support so that Women-Farmer's Center will become as a base camp for young women farmers to participate in agriculture and rural community and increase its economic effect for the nation in the future.

• Journal of Korean Society of Forest Science
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• v.95 no.2
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• pp.194-202
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• 2006

The purpose of this study was to provide necessary requirements on accreditation for forest culture and recreational interpretive programs in order to set up the enforcement regulations of the Forest Culture and Recreation Act. The intention of this study was to contribute to raising the qualitative standards of forest interpreters through the adoption of accreditation procedures for forest culture and recreational education programs, to establish detailed standards regarding the accreditation of forest interpreter training courses, and to provide materials and resources useful in creating forestry policies regarding forest interpreters and forest culture and recreation. This study examined five topics: 1) To establish conceptual understanding of the accreditation procedures for forest culture and recreation interpretive programs, 2) To understanding the present status of training programs and courses related to accreditation procedures for forest culture and recreation interpretive programs, and examining the status of current forest interpreter training courses, 3) To propose an operational scheme for accreditation procedures for forest culture and recreation programs, and develop the goal of an operation of the accreditation procedures for forest culture and recreation programs, 4) To propose an operational scheme for accreditation procedures for forest culture and recreational education programs, and the operational scheme for forest interpreter training courses, and 5) To establishing a commission to evaluate the accreditation procedures for forest culture and recreation programs and operating guidelines.

• Journal of Distribution Science
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• v.16 no.9
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• pp.35-41
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• 2018

Purpose - The purpose of was paper is to do a conceptual analysis of the determination of local superior commodities using the location quotient technique and an analysis of the contribution of superior products to the regional economy. The results of determining superior commodities are used to determine regional product specialization and its contribution to the regional economy. Local superior commodities are dynamic and selected based on the potential to increase regional income. Research design, data, and methodology - This study used location quotient techniques and regional economic analysis with annual data rocks, production data in the agriculture, plantation, fishery and forestry sectors. Local superior commodities are calculated to contribute to regional economists. Result - The findings obtained showed the location quotient technique can determine local superior commodities and local superior commodities contributed positively to the regional economy. The test results were carried out in Jember Regency, East Java Province, Indonesia. Conclusion - The ass well main commodities of Jember Regency are tobacco. Tobacco commodities contribute positively to the regional economy, they were not significantly due to affected by the low of commodity prices in harvest. The low prices indicate the strength of the weak bargaining position of farmers towards the market.

• Management & Information Systems Review
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• v.35 no.3
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• pp.273-287
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• 2016

This study aimed to clarify the effect of information technology (IT) education on the efficiency and effectiveness of working processes among agriculture corporations. Survey data on information levels from 222 agriculture corporations were collected from the Korean Agency of Education, Promotion, and Information Service in Food, Agriculture, Forestry, and Fisheries (EPIS) for a governmental white paper. Structural equation modeling was used for analysis. This study found that IT education increases the ratio of the use of information systems in working processes, especially given the use of data accumulated through information and communications technologies (ICT). The findings of this study suggest that the use of ICT data as an aspect of IT education is beneficial for the agricultural sector.

• The Korean Journal of Mycology
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• v.38 no.1
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• pp.1-4
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• 2010

Orchid mycorrhizal fungi(OMF) were examined in roots of the two threatened orchid species, Cypripedium japonicum and C. macranthum. The morphological characteristics of mycorrhizal colonization in the roots of two orchid species were observed. OMF colonized in the roots of two species were identified using molecular analysis. DNA from the root was extracted and amplified internal transcribed spacer(ITS) region using basidiomycete ITS primers, ITS1-OF and ITS4-OF. Four species belonging to Tulasnellaceae in roots of C. japonicum and two species of Tulasnellaceae and one basidiomycetous species was found in roots of C. macranthum.

• Journal of Forest and Environmental Science
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• v.16 no.1
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• pp.105-118
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• 2000

The study is focused on comparing the perception of forest investment by two types of forest owners, ordinary forest owners (OFOs) and forestry successors and model forest owners(FSMFOs). The results show that FSMFOs' perception of forest investment is relatively high compared with OFOs' perception. The details are as follows; 1. In the general status, the education and income levels of FSMFOs are higher than those of OFOs, and FSMFOs are engaged in various kinds of jobs while OFOs are inclined to lean toward farming. 2. Of the motives to own forests, partial-purchase and -inheritance is the most of FSMFOs(41.2%) and inheritance the most of OFOs(40.9%) 3. The number of FSMFOs who had made forest investment and applied government support is more than that of OFOs. 4. FSMFOs know government support better than OFOs do, while both show lukewarmness on weather the support is carried out according to the rules.