• Title/Summary/Keyword: Forensic service

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Sensitivity study of the Yfiler® PLUS PCR Amplification Kit in forensic casework samples (법과학 현장시료에서 Yfiler® PLUS PCR Amplification Kit의 민감도 연구)

  • Jung, Ju Yeon;Kim, Kyoung Sook;Park, Sun Wha;Lim, Si Keun;Lee, Dong Sub;Lee, Yang Han
    • Analytical Science and Technology
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    • v.29 no.1
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    • pp.43-48
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    • 2016
  • A variety of Y-STR analysis kits have been developed and used in the forensic field. Prior to the forensic application of a new kit, laboratory validation and sensitivity tests are essential processes in selecting suitable alternatives and for assuring that standard operating procedures are followed. In this paper, we have performed a sensitivity study of a new commercial kit, the Yfiler® PLUS PCR Amplification Kit (Yfiler plus kit, released in 2014) by comparing it with the AmpF/STR® YfilerTM PCR Amplification Kit (Yfiler kit, released in 2004). The Yfiler plus kit includes the 17 Y-STR loci of the Yfiler kit and has been supplemented with 10 new Y-STR loci. First, we analyzed the sensitivity difference between the two kits using commercial control DNA 2800M and 007. In addition, we compared the detection rate between the two kits from the 16 selected forensic casework samples of less than 0.5 ng concentrations. The results show that the sensitivity and detection rate of the Yfiler plus kit are higher than the corresponding rates of the Yfiler kit. In addition, we were able to obtain more Y-STR profiles with the use of the new kit. Thus, we suggest that Yfiler plus kit is a more effective forensic tool to detect Y-STR profiles from forensic casework samples of low concentrations.

Unexpected Death by Sepsis of Staphylococcus aureus with Infective Endocarditis and Paravertebral Abscess in a Fisherman during Sailing out for Fish: An Autopsy Case (심내막염과 척추 주위 농양을 동반한 황색포도알균에 의한 출어 중의 선상 패혈증 사망: 증례 보고)

  • Kim, Youn Shin;Hwang, In Kwan;Moon, Seohyun;Park, Ji Hye;Lee, Young Seok
    • The Korean Journal of Legal Medicine
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    • v.42 no.4
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    • pp.153-158
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    • 2018
  • Staphylococcus aureus is an important cause of human infections, and it is also a commensal that colonizes the nose, axillae, vagina, throat, or skin surfaces. S. aureus has increasingly been recognized as a cause of severe invasive illness, and individuals colonized with this pathogen are subsequently at increased risk of its infections. S. aureus infection is a major cause of skin, soft tissue, respiratory, bone, joint, and endovascular disorders, and staphylococcal bacteremia may cause abscess, endocarditis, pneumonia, metastatic infection, foreign body infection, or sepsis. The authors describe a case of a fisherman who died of sepsis on a fishing boat during sailing out for fish. The autopsy shows paravertebral abscess, pus in the pericardial sac, infective endocarditis with vegetation on the aortic valve cusp, myocarditis, pneumonia and nephritis with bacterial colonization, and also liver cirrhosis and multiple gastric ulcerations.

Forensic Engineering Study on Structural Integrity Evaluation of Scaffolding System Tower using ANSYS (ANSYS를 이용한 스캐폴딩 시스템 타워 구조 건전성 평가에 관한 법공학적 연구)

  • Kim, J.H.;Kim, E.S.;Park, W.S.;Moon, B.S.;Goh, J.M.;Park, N.K.;Yoon, K.B.;Cho, S.W.
    • Journal of the Korean Society of Safety
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    • v.28 no.6
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    • pp.42-48
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    • 2013
  • Forensic engineering is the application of engineering principles covering the investigation of constructed facilities and systems that fail to perform as intended, causing personal injury or damage to property, environmental, economy etc. In the year 2012, two collapsed accidents of the large scaffolding system in national thermal power station occurred one after another, causing many casualties. In this study, we had performed to investigate the collapsed accident of scaffolding system occurred in the a thermal power station of two accidents. First, the investigation about the collapsed accidents site had performed to understand collapsed state and structures of the scaffolding system. Second, reviewing the materials concerning about the applied weight on the scaffolding system had performed. The applied weight is sum of the weights of the 15 workers, additional materials for coating work and dispersed and loaded shot ball on the foothold etc. the applied weight that calculated exceed more three times than the safe working load. Third, we had confirmed the install state of the materials of the scaffolding system by reviewing the quantity of the materials on the manual and the real system. Last, structural analysis had performed to evaluate structural integrity of the scaffolding system using Ansys. Through a series of this processes, the definite accidents causes of the collapsed scaffolding system revealed. Through these studies, the collapse accident that may occur in the scaffolding system in thermal power station can be minimized by performing specialized and systematic investigation on the accidents in terms of Forensic engineering.

The Application of Paewinsky et al.'s Age Estimation Method to Periapical Radiographs (치근단 방사선 사진에서 Paewinsky 연령추정법 적용에 대한 연구)

  • Roh, Byung-Yoon;Seo, Jeong-Uk;Kim, Chang-Gyum;Choi, Chang-Un;Lee, Won-Joon;Lee, Sang-Seob
    • The Korean Journal of Legal Medicine
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    • v.42 no.4
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    • pp.141-145
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    • 2018
  • There have been many radiographic studies on age estimation that evaluate reduction in size of dental pulp cavity with secondary dentin formation. The Paewinsky method reported high accuracy in estimating ages by measuring the width of the pulp cavity in panoramic radiographs. The aim of this study was to evaluate the application of the Paewinsky method to digital periapical radiographs. This study was conducted on 103 cases that reported to the Section of Human Identification of the National Forensic Service. The age was calculated by applying the Paewinsky method that measures the root and pulp canal width at three points in a tooth. The estimation results were compared with those calculated by the Johanson method. When the Paewinsky models were applied to digital periapical radiographs, the errors were significantly greater as compared to the original study. The errors of the maxillary second premolar and mandibular lateral incisor were greater than those of the maxillary central incisor, lateral incisor, mandibular canine, and first premolar. Furthermore, errors of the age estimation models in level C were greater than those in levels A and B. This study could be a reference for the application of the Paewinsky method to digital periapical radiographs.

Method validation of detecting ethanol metabolites (EtG, EtS) in post-mortem spleen (비장 조직에서 에탄올 대사체(EtG, EtS)를 검출하는 방법과 유효성 확인)

  • Kim, Soo-Min;Jo, Young-Hoon;An, Song-Hie;Lee, Woo-Jae;Kwon, Mia
    • Analytical Science and Technology
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    • v.34 no.3
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    • pp.115-121
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    • 2021
  • Ethyl glucuronide (EtG) and ethyl sulfate (EtS), which are ethanol metabolites, are direct indicators of ethanol intake; they have been studied in a variety of biological samples in forensic science. It is necessary to analyze ethanol metabolites to determine whether the ethanol detected in autopsy cases was due to alcohol consumption before death or due to the ethanol produced from post-mortem decay. In general, EtG and EtS are detected in the blood together with ethanol; however, it may be difficult to secure blood depending on the extent of decay. Therefore, the aforementioned method should be replaced by detecting the ethanol metabolites using tissue biological samples. In this study, we determined the optimal experimental conditions for detecting EtG and EtS from spleen samples using Liquid Chromatography - Tandem Mass Spectrometry (LC-MS/MS). Herein, the test method was validated, and an analysis method was applied to the actual autopsy cases.

The Moderating Role of Binge Drinking on the Self-Assertiveness-Sexual Victimization Relationship among College Students (대학생의 자기주장과 성폭력 피해에서 폭음의 조절역할)

  • Hong, Hyeon-gi;Kim, Hee-song;Lee, Jung-eun;Jung, Sue-hyun;Ji, Hyung-ki;Kim, Ki-pyoung;Pyo, Chu-yun;Kim, Jong-han;Hyun, Myoung-Ho
    • Stress
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    • v.25 no.2
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    • pp.93-97
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    • 2017
  • Backgound: The present study was designed to investigate the moderating role of binge drinking on the relationship between self-assertiveness and sexual victimization among college students (N=393, male: 161, female: 232). We hypothesized that the relationship between self-assertiveness and sexual victimization is meaningful only when binge drinking is low, not high. Methods: Using an online site, participants filled out a packet of questionnaires for demographic information, their amount of unwanted sexual contact experience, and their rate of alcohol consumption. Results: In the result, binge drinking moderated the relationship between self-assertiveness and sexual victimization. For the high binge drinker group, high self-assertiveness was not a statistically significant predictor of sexual victimization. Conclusions: Although self-assertiveness is known to be a protective factor against sexual victimization, our results imply that self-assertiveness alone is not enough to lower sexual victimization. Binge drinking and self-assertiveness are considered together in sexual violence prevention programs.

The Study on Evaluation of Human Body Injury by Explosion of Portable Butane Gas Range (부탄연소기 폭발로 인한 인체 상해 평가에 관한 연구)

  • Kim, Eui Soo;Shim, J.H.;Kim, J.P.;Park, N.K.
    • Journal of the Korean Society of Safety
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    • v.31 no.3
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    • pp.60-67
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    • 2016
  • The gas leak and explosion accident is able to give a fatal injury to nearby people from the explosion center and interest in effect of the explosion on the human body is increased. Accidents by Portable Butane Gas Range of a gas explosion accident occupy the most share. As a result, the injury on the human body frequently occur. However, It is situation that are experiencing difficulties in consequence analysis of explosion accidents owing to shortage of explosion power data and lack of research on the effect of the human body by the gas explosion. This paper acquire human injury data by performing the actual explosion experiment with Portable Butane Gas Range and evaluate power by explosion and effect of explosion on the human body to perform explosion simulation with LS-DYNA program. It is intended to contribute to the exact cause of the accident investigation and the same type of accident prevention.

Simulation of Indoor Shooting Range Fire in Busan by Using FDS (FDS를 이용한 부산 실내사격장 화재 시뮬레이션)

  • Cho, Young-Jin;Seo, Young-Il;Moon, Byung-Sun;Kim, Sung-Jin;Jeon, Woo-Jeong
    • Proceedings of the Korea Institute of Fire Science and Engineering Conference
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    • 2011.11a
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    • pp.143-146
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    • 2011
  • 2009년 11월 14일 발생한 부산 실내사격장 화재와 관련하여 조사내용을 토대로 화재 시뮬레이션용 전선유체역학 모델인 FDS를 이용하여 재현하였으며, 시뮬레이션 결과와 화재당시 녹화된 CCTV 영상을 비교하였다. 2층 발사실에서 시작된 화재는 급격히 연소가 진행되어 약 3초 후에 휴게실로 확산되며, 약 7초 후에는 계단 및 1층 홀을 지나 사람들이 통행하는 인도에까지 연기가 분출되는 것으로 나타났다. 시뮬레이션 결과는 2층 발사실과 휴게실에 녹화된 CCTV 영상 및 1층 홀 옆의 귀금속가게에 녹화된 CCTV 영상과 거의 일치하였다.

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Application and evaluation of machine-learning model for fire accelerant classification from GC-MS data of fire residue

  • Park, Chihyun;Park, Wooyong;Jeon, Sookyung;Lee, Sumin;Lee, Joon-Bae
    • Analytical Science and Technology
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    • v.34 no.5
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    • pp.231-239
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    • 2021
  • Detection of fire accelerants from fire residues is critical to determine whether the case was arson or accidental fire. However, to develop a standardized model for determining the presence or absence of fire accelerants was not easy because of high temperature which cause disappearance or combustion of components of fire accelerants. In this study, logistic regression, random forest, and support vector machine models were trained and evaluated from a total of 728 GC-MS analysis data obtained from actual fire residues. Mean classification accuracies of the three models were 63 %, 81 %, and 84 %, respectively, and in particular, mean AU-PR values of the three models were evaluated as 0.68, 0.86, and 0.86, respectively, showing fine performances of random forest and support vector machine models.