• 한국산림과학회지
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• 제97권2호
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• pp.156-164
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• 2008

Recognizing the importance of the multi-purpose management of natural deciduous forest, this study was carried out to implement the partial cutting for stand regulation to examine agroforestry practice as well as other concurrent forest resource production, and to investigate the changes in stand characteristics and understory vegetation in a silvopasture practiced natural deciduous stand in the Research Forest of Kangwon National University, Korea. Three different partial cutting intensities (68.1%, 48.6%, and control) were performed in the unmanaged natural deciduous stand in order to improve the growing condition, especially light condition, for introducing some commercial herbaceous plants on the forest floor to establish agroforestry and/or silvopastoral system. Dominated by Quercus varibilis Blume (50.5%) and Quercus dentata Thum. ex Murray (42.6%), eight tree species were composed of the study forest, including poles of Pinus desiflora Siebold & Zucc and sapling of Pinus Koraiensis Siebold & Zucc. The total of 87 (13 tree species, 12 shrub species, 58 herbaceous species, and 4 woody climbers) vascular plant species were observed in study site after partial cutting treatments, while that of before partial cutting was 53 species (14 tree species, 8 shrubs species, 30 herbaceous species, and 1 woody climbers). The proportion of life form spectra in plot B was Mi (28.4%)-Na (23.0%)-Ge (17.5%)-Ch (10.8%)-He (9.5%)-MM (6.7%)-Th (4.1%). No statistically significant differences were observed in changes of life form spectra from before to after partial cutting treatment and among partial cutting gradients in this study. Partial cutting and scratching for forage sowing made plants invade easily on the forest floor, and light partial cutting (LPC) plot (500 stems/ha) had much higher number of undersory species than those of heavy partial cutting (HPC) plot (310 stems/ha) and control plot (1,270 stems/ha).

• 농업과학연구
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• 제4권2호
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• pp.178-190
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• 1977

충남(忠南) 부여군(扶餘郡) 은산면(恩山面) 라령리(羅嶺里) 소재(所在) 본대학(本大學) 연습림(演習林)은 온대(溫帶) 중부(中部)의 참나무류(類)의 극성상(極盛相) 임분(林分)이 대부분(大部分) 파양(破壤)되어 남면(南面)과 산봉(山峰)을 연결(連結)하는 능선(稜線) 부분(部分)은 소나무 단순림(單純林)의 준극성상(準極盛相)을 이루고 있다. 식생(植生)은 비교적(比較的) 다양(多樣)하며 주요(主要) 수종(樹種)은 소나무, 상수리나무, 굴참나무, 갈참나무, 졸참나무, 비목나무 굴피나무등이며 목본 53과 203종, 초본 49과 128 종이다. 이들 식물(植物)을 이용도(利用度)에 따라 분류(分類)하면 다음과 같다. 식용(食用) 식물(植物) 60과 154종, 약용(藥用) 식물(植物) 63과(科) 143종(種) 초자원(草資源) 식물(植物) 51과 140종, 관상(觀賞) 식물(植物) 32과(科) 78종(種), 용재수종(用材樹種) 12과 27종등이다.

• The Plant Pathology Journal
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• 제26권3호
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• pp.216-222
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• 2010

Fusarium proliferatum is the causal agent of stalk and root rot disease of maize, foot rot disease of rice, basal and root rot disease of onion and knife cut disease of sugarcane in Iran. In recent years, incidence and severity of these diseases have been increased in Iran. Fifty seven F. proliferatum single-spore isolates collected from diseased maize, rice, onion and sugarcane plants at different areas were used to study genetic diversity by determination of vegetative compatibility groups (VCGs). Chlorate-resistant nitrate non-utilizing (nit) mutants were recovered from selected isolates of F. proliferatum and used in complementation tests. All isolates in which both nit1 and NitM (or nit3) mutants were recovered, demonstrated self-compatibility. Vegetative compatibility tests by pairing nit mutants identified 30 VCGs among 57 isolates. Twenty-three isolates belonged to singlemember VCGs and the remaining 34 isolates, belonged to other seven multimember VCGs. Segregation of F. proliferatum isolates obtained from various area and host plants into different VCGs in Iran is reported for the first time. In this study, none of isolates obtained from rice complemented with any other isolates from onion and sugarcane and, non complementation occurred between onion and sugarcane isolates. Also, only one complementation occurred between one isolate of maize and one isolate of sugarcane and rice. Thus, a correlation between VCGs grouping and host preferences was founded. It is concluded that natural populations of F. proliferatum in Iran are probably genetically divergent and include isolates representing a potential risk for disease development.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.106-106
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• 2017

The soybean [Glycine max (L.) Merr.] is one of the most important crop resources worldwide as food and forage. It is also important and valuable that to hold crop resources to have high genetic diversities. Recently, a core collection has been constructed in many plants to preserve the genetic resources of various plants. A core collection is small population to represent the genetic diversity of the total collection, and is of strategic importance as they allow the use of a small part of a germplasm collection that is representative of the total collection. Here, we developed the core collection consisting of 816 accessions by using approximately 180,000 (180K) single nucleotide polymorphisms (SNPs) developed in previous study. In addition, we performed genetic diversity and population structure analysis to construct the core collection from entire 4,392 collections. there were excluded sample call rates less than 93% and duplicated samples more than 99.9% according to genotype analysis using 180K SNPs from entire collections. Furthermore, we were also excluded natural hybrid resources which Glycine max and Glycine soja are mixed in half through population structure analysis. As a result, we are constructed the core collection of genetic diversity that reflects 99% of the entire collections, including 430 cultivated soybeans (Glycine max) and 386 wild soybeans (Glycine soja). The core collection developed in this study should be to provide useful materials for both soybean breeding programs and genome-wide association studies.

• Asian-Australasian Journal of Animal Sciences
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• 제12권2호
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• pp.209-214
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• 1999

Grazing preference, voluntary dry matter intake, stocking rate and digestibility of forage were investigated or sheep grazed in mature oil palm plantations in Malaysia. Dry matter intake (DMI) was estimated using the chromium sesquioxide marker method. The sheep were allowed to graze about 5 hours/day in oil palm plantations of age ranging from 9 to 21 years old. The crude protein, crude fibre and ash contents of selected herbage were 13.1 %; 24.6 %; and 8.3 %, respectively, while the gross energy (GE) and the metabolisable energy (ME) were 16.9 and 6.0 MJ/kg DM, respectively. Broad leaf plants and grasses were normally found in plantation of all ages. Legumes and oil palm seedlings formed the smallest group in the whole mixture. Fern content increased in older plantations. Legumes, oil palm seedlings, fern, broad leaves and grasses were 0.4, 1.3, 11.4, 28.0 and 59.0 %, respectively of total herbage. Grasses showed the highest preference index followed by broad leaf plants, legumes, oil palm seedlings and ferns in that order. The DMI and the metabolisable energy intake (MEI) of sheep at 6, 8, 10, 12 and 14 months of age were 64.8, 65.0, 65.3, 65.6 and 67.4 g/kg $W^{0.75}$ (p<0.05) and 0.39, 0.40, 0.40, 0.40 and 0.41 MJ/kg $W^{0.75}$, respectively. The average stocking rate was 4.3 sheep/ha. It was concluded that the performance of the sheep could be improved by increasing the daily grazing period and also by appropriate concentrate supplementation.

• Asian-Australasian Journal of Animal Sciences
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• 제20권3호
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• pp.393-398
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• 2007

The present study was carried out to investigate the serum minerals profile in buffaloes (Bubalus bubalis) suffering from parturient haemoglobinuria (PHU) along with minerals profile of soils and fodders from the disease prone areas and their interrelationships. Serum samples were collected from 60 each of healthy and PHU affected buffaloes randomly selected from field cases. Serum samples were collected from each animal. Fifty composite soil samples were collected where PHU was prevalent. Fifty samples of fodders including leaves and stems being fed to the diseased buffaloes were collected. The difference in the levels of calcium and potassium between upper and lower soil surface of disease prone areas under study were statistically non-significant. The mean values of phosphorous, copper, iron, selenium and molybdenum in upper soil surface were significantly (p<0.05) higher than in lower soil surface. None of the fodders offered to the diseased animals met the dietary requirements of phosphorus and copper whereas none of the fodders was deficient in potassium, iron and selenium rather were having excess of potassium, iron and selenium. The concentration of calcium was adequate in lucerne, berseem, sarson and sorghum, while maize, sugarcane and wheat straw did not meet the required levels for dairy animals. Molybdenum contents in all fodders were adequate to meet the dietary requirements of the dairy buffaloes. Serum phosphorus, copper and selenium were significantly (p<0.001) lower whereas potassium, iron and molybdenum in buffaloes suffering from PHU were significantly (p<0.001) higher than in healthy buffaloes. It was concluded that phosphorous deficient soils play a major role by transferring this deficiency to plants and ultimately reaching to animals where hypophosphataemia is a consistent finding.

• 한국환경복원기술학회지
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• 제21권3호
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• pp.47-56
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• 2018

Research was initiated to investigate germination characteristics and germination pattern of 14 herbaceous plant entries used on the road slope during 30 days. An alternative germination condition for 14 herbaceous plant entries required by International Seed Testing Association(ISTA) was applied in the experiment, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$. Significant differences were observed in the first germination rate(0.3%~40.7%)and in the final germination rate(7.7%~93.3%). Days to the first germination(2~8days), days to the 30% germination(2~6days) and days to the peak germination(6~18days) were different among 14 herbaceous plant entries in the study. From this result, we could find out higher final germination rate of 14 herbaceous plant entries in the following order; First, forage crops and cool-season turfgrasses; Second, herbeceous flowers; Third, wild plants. We could also divide germination rate among 14 herbaceous plant entries as 6 groups(I;very high, II;high, III;medium high, IV;medium low, V;low, VI;very low) based on the final germination rate and divide germination speed as 5 groups(A;very fast, B;fast, C;normal, D;slow, E;very slow)based on days to the peak germination. Considering germination characteristics and pattern of 14 herbaceous plant entries Medicago sativa, Lolium perenne, Festuca arundinacea and Cosmos sulphureus were regard as dominating species while Lespedeza cuneata, Silene armeria, Lotus corniculatus var.japonicus, Coreopsis tinctoria and Centaurea cyanus as competitive species following dominating species. However, Chrysanthemum burbankii, Pennisetum alopecuroides, Chrysanthemum boreale., Artemisia princeps var. orientalis and Arundinella hirta were not almost expected to emerge.

• Asian-Australasian Journal of Animal Sciences
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• 제32권11호
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• pp.1686-1694
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• 2019

Objective: Lignin plays a relevant role in the inhibition of cell wall (CW) structural carbohydrate degradation. Thus, obtaining accurate estimates of the lignin content in tropical plants is important in order to properly characterize the mechanism of lignin action on CW degradation. Comparing conflicting results between the different methods available for commercial use will bring insight on the subject. This way, providing data to better understand the relationship between lignin concentration and implications with tropical forage degradation. Methods: Five grass species, Brachiaria brizantha cv $Marand{\acute{u}}$, Brachiaria brizantha cv $Xara{\acute{e}}s$(MG-5), Panicum maximum cv Mombaça, Pennisetum purpureum cv Cameroon, and Pennisetum purpureum cv Napier, were harvested at five maturity stages. Acid detergent lignin (ADL), Klason lignin (KL), acetyl bromide lignin (ABL), and permanganate lignin (PerL) were measured on all species. Lignin concentration was correlated with in vitro degradability. Results: Highly significant effects for maturity, lignin method and their interaction on lignin content were observed. The ADL, KL and ABL methods had similar negative correlations with degradability. The PerL method failed to reliably estimate the degradability of tropical grasses, possibly due to interference of other substances potentially soluble in the $KMnO_4$ solution. Conclusion: ADL and KL methods use strong acid ($H_2SO_4$) and require determination of ash and N content in the lignin residues, therefore, increasing time and cost of analysis. The ABL method has no need for such corrections and is a fast and a convenient method for determination of total lignin content in plants, thus, it may be a good option for routine laboratory analysis.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.234-234
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• 2022

Gene-editing is currently one of the most popular technologies in recent years. Development of the new crop using the gene editing have advantage of improved accuracy and efficiency compared with conventional breeding. Soybean (Glycine max L.) is one of the most important crops worldwide used as food and forage. We tried to establish a system for breeding improvement of soybean through gene-editing technology. For the gene-editing system of soybean, i) selection of efficiency gRNA of targeted gene, ii) efficient genetic transformation of the selected gRNA, iii) selection of trans-clean mutant is essential. First of all, we investigated the selection conditions of gRNA with high editing efficiency of targeted gene using isolated protoplast of soybean. Furthermore, we performed the Agrobacterium-mediated genetic transformation of various soybean cultivars. We identified the tissue culture ability in 23 soybean cultivars for genetic transformation of soybean. The six cultivars with high tissue culture ability were selected and confirmed the transgenic plants in four cultivars. Finally, we established a speed-breeding system as a powerful tool for the fast selection of trans-clean mutants from transgenic plants. Our laboratory will provide the valuable system for improvement of soybean by the gene-editing technology.

• 한국토양비료학회지
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• 제14권4호
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• pp.224-229
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• 1982

울릉도자생(鬱陵島自生) 섬바디의 광합성(光合成) 및 호흡(呼吸)에 대(對)한 광도(光度) 및 온도(溫度)의 영향(影響)을 조사(調査)하였다. 1. 광합성(光合成)의 광포화점(光飽和点) 및 광보상점(光補償点)은 $20^{\circ}C$에서 각각(各各) 34~38klux와 4~6klux이고 광포화점(光飽和点)에서의 광합성속도(光合成速度)(호흡량(呼吸量)을 제(除)한)는 9~12mg $CO_2/dm^2/hr.$이었다. 2. 광합성적온(光合成適溫)은 $20^{\circ}C$이고 2온도범위($20{\sim}30^{\circ}C$, $30^{\circ}C{\sim}400^{\circ}C$)에서 광합성(光合成)의 $Q_{10}$치(値)는 0.8 및 0.9이고 호흡(呼吸)의 $Q_{10}$치(値)는 1.6 및 1.7이었다. 3. 자생지(自生地)의 환경조건(環境條件)에 적응(適應)하여 해발고도(海拔高度)가 높은 장소(場所)에서 자생(自生)한 식물본의 광보상점(光補償点)이 낮았다. 이상(以上)의 결과는 우리나라의 본과 가을이 섬바디생육(生育)에 적합(適合)함을 알 수 있고 하절기(夏節期)의 생육(生育)이 저조할 것임으로 다른 목초(牧草)와의 혼식재배(混植栽培)가 이상적(理想的)이라고 생각된다.