• Advances in Traditional Medicine
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• v.5 no.4
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• pp.294-300
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• 2005

Uncaria Tomentosa and Maytenus are known to have anti-inflammatory and anti-rheumatoidal effects after either a single application or application over a short-term period. We applied these natural products to Wister rats every day for two weeks and investigated the effects of this long-term application on inflammation. This was done by measuring footpad edema, which was induced by a locally injected carrageenan. There was a dramatic reduction in edema in both U. Tomentosa- and Maytenus-treated rats; furthermore, the reduction lasted as long as three days for rats treated with U. Tomentosa. When the Balb/C mice underwent similar treatment for one month, the level of IgM in the blood of U. Tomentosa-treated mice decreased while the level of IgG in Maytenus-treated mice increased. This suggests that the long lasting effects of U. Tomentosa may be related to a low level of IgM and the subclass switch from IgM to IgG. Since the anti-inflammatory effects of U. Tomentosa lasts for three days, it may prove useful in treating rheumatoid arthritis when applied for an extended period of time, especially since this product is known to have minimal side effects.

• YAKHAK HOEJI
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• v.48 no.6
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• pp.345-351
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• 2004

ln the present work to determine effect of a Streptococcus pneumoniae conjugate vaccine, S.pneumoniae capsule attached to the surface protein (JY-Pol) was ex amined. This JY-Pol contained approximately 92% and 6% carbohydrate and protein, respectively. Gel electrophoresis revealed the presence of the surface protein in the JY-Pol. By the double immunodiffusion and isotyping ELISA analyses, administration of JY-Pol that was adsorbed to alum adjuvant (JY-Pol/Alum) into mice induced IgM, IgG, and IgA specific for the S.pneumoniae capsule. The ATCC capsular polysaccharide adsorbed to alum (ATCC-Pol/Alum) provoked only IgM in mice. In survival tests, mice that were immunized with the JY-Pol/Alum before intravenous challenge with live S.pneumoniae survived entire period of 46 day-observation, whereas all mice that received ATCC-Pol/Alum or only diluent instead of the vaccination died within 5 and 12 days, respectively. Results from footpad-edema test showed that JY-Pol/Alum formula provoked the cellular immunity as determined by swelling of the mouse footpad. These data indicate that the naturally conjugated JY- Pol enhances resistance of mice against disseminated pneumococcal disease due to S.pneumoniae by both humoral and cellular immune responses.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.6 no.1
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• pp.31-52
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• 1993

The object of thes research is to lucidate the clinical effects of Gami-Cheongshimyeonjayeum on the anti-allergic and gimmune response to rats and mice. The obtaind results of Gami-Cheongshimyeonjayeum administration are as follows : 1. The cytotoxic index increased slightly at over concentration $1{\times}10^{-2}/ml$, but it is thought that the cytotoxic effect show no significant value under the concentration $1{\times}10^{-3}/ml$. 2. Gami-Cheongshimyeonjayeum water extract seems to suppress the leakage volume of Evans blue to decrease the vascular permeability. 3. Gami-Cheongshimyeonjayeum show the inhibitory effect of footpad edema. 4. Protein leakage is inhibited by the administration of Gami-Cheongshimyeonjayeum. 5. The delayed type hypersensitivity to SRBC is suppressed at the 24hours after the administration of Gami-Cheongshimyeonjayeum on the experimental group 1, 2. 6. Hemolysin and hemagglutinin titer, and RFC increase to bulid up the immune function.

• Journal of Veterinary Clinics
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• v.27 no.4
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• pp.445-449
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• 2010

A dog with a history of long-term phenobarbital administration presented to Seoul National University Hospital for Animals with clinical signs of footpad ulceration and facial ulcerative dermatitis. Skin lesions (facial/footpad dermatitis), histopathologic signs (parakeratotic hyperkeratosis, edema and hyperplasia of the epidermis), and liver involvement (increased plasma liver enzymes and nodular lesions) suggested a diagnosis of superficial necrolytic dermatitis (SND). The patient died suddenly at home of unknown cause after 8 months medical treatment, but intravenous amino acid supplementation and supportive hepatic medication proved as effective for the skin conditions.

• YAKHAK HOEJI
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• v.58 no.5
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• pp.343-348
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• 2014

In the present studies, we examined effect of chamomile flowers extract (CH-Ex), which has traditionally been used as antiphlogistics in Europe for many centuries, against Candida albicans-caused septic arthritis. Candida albicans is a major etiological agent among fungal septic arthritis. This effect was investigated in a murine model of the septic arthritis. That is, mice that were given an emulsion form of C. albicans cell wall (CACW) via footpad route were treated intraperitoneally with the CH-Ex for 3 times every 3 days. Degrees of the footpad-swellings were measured with dial gauger. Data showed that the CH-Ex resulted in the reduction of swelling. For instance, at Day 9 when swelling reached the highest peak, there was up to app. 60% reduction of edema in mice injected with the CH-Ex, compared to that of the control mice that received no treatment (P<0.05). This therapeutic anti-arthritic activity appeared to be mediated by inhibitions of NO (nitric oxide) production from activated RAW264.7 macrophages and proliferation of Con A-treated T lymphocytes. Analysis by HPLC revealed that the CH-Ex contained eight polyphenolic compounds including chlorogenic acid (CRA) and rutin. We have reported the CRA and rutin respectively have the anti-arthritic activity. This correlation implicates that CRA and rutin in the CH-Ex may be responsible for the activity. Combined all together, the CH-Ex has anti-arthritic activity against C. albicans-caused septic arthritis, possibly by inhibiting NO production and proliferation of T cells. This activity seems to be contributed by, at least, CRA and rutin among the compounds in the CH-Ex.

• Journal of Veterinary Clinics
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• v.32 no.5
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• pp.445-448
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• 2015

A 5 month old Korean domestic short haired male kitten (weighing 1.7 kg) was presented with primary complaints of upper respiratory disease (URD) signs and skin ulceration and edema on face, feet and footpad with lameness. Diagnostic test revealed leukopenia, lymphopenia, pancreatitis and feline calicivirus (FCV) infection. Diagnosis of virulent systemic FCV (VS-FCV) was made on clinical signs, isolation of calicivirus via PCR and exclusion of other causes of acute upper respiratory disease. Therapeutic strategies were directed to lessen URD signs and to treat secondary bacterial infection and antiviral infection. One month after this therapy, skin lesions on face and feet and URD signs were much improved, although the lameness persisted mildly. To author's best knowledge, this is the first case reporting VS-FCV infection in a kitten in Korea.