• Journal of Apiculture
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• v.34 no.1
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• pp.47-55
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• 2019

Honey bees are a vital part of the food chain as the most important pollinators for a broad palette of crops and wild plants. The climate change and colony collapse disorder (CCD) phenomenon make it challenging to develop ICT solutions to predict changes in beehive and alert about potential threats. In this paper, we report the test results of the bee-counting system which stands out against the previous analogues due to its comprehensive components including an improved dual infrared sensor to detect honey bees entering and leaving the hive, environmental sensors that measure ambient and interior, a wireless network with the bluetooth low energy (BLE) to transmit the sensing data in real time to the gateway, and a cloud which accumulate and analyze data. To assess the system accuracy, 3 persons manually counted the outgoing and incoming honey bees using the video record of 360-minute length. The difference between automatic and manual measurements for outgoing and incoming scores were 3.98% and 4.43% respectively. These differences are relatively lower than previous analogues, which inspires a vision that the tested system is a good candidate to use in precise apicultural industry, scientific research and education.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.29 no.1
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• pp.27-33
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• 2023

An optimized design of the transportation insulated box must be considered to control the thermal damage in order to maintain the fresh condition for temperature-sensitive medicine and frozen food safety. The inside temperature of the insulated box is a natural convection enclosure state, thermal stratification naturally occurs as time passes in case of with outside heat load. The latent heat material (LHM) placement inside the box maintains the target temperature of the product for temperature fluctuations during transport, and LHM application is a common and efficient method. In this work, inside temperature stratification in an insulated box depending on the LHM pack position is numerically simulated and experimented. The insulated box is made up of vacuum insulation panel (VIP), and LHM modules are placed over six faces inside the box, with the same weight. The temperature curves for 72 hrs as experiment results clearly show the temperature stratification in the upper, middle, and lower at the LHM melting time region. However, the temperature stratification state is uniformly changed in accordance with the condition of the upper and lower placement weight of the LHM pack. And also, the temperature uniformity by changed placement weight of LHM has an effect on maintaining time for target air temperature inside the box. These results provide information on the optimized design of the insulated box with LHM.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.2
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• pp.123-132
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• 2008

The problems caused by new biological organisms in Ogasawara Island of Japan include : (1) loss of native species' habitats and extinction of native species due to the multiplication of invasive species; (2) reduction of native species and confusion in the food chain in result of invasive species' predation; (3) confusion in genes due to hybridization of new and native plants; and (4) destruction of ecosystem and erosion of earth due to rapid increase of invasive species. The natural ecosystem of preserved areas in Korea is constantly confused and destroyed by new animals and plants, but Korea does not has any solutions because new animals and plants rarely affect human lives. Invasive species are manually introduced by humans and are considered disasters caused by humans. Korea is in desperate need of campaigns as the general public does not understand the seriousness of the destruction and confusion of natural ecosystem caused by new biological organisms and their indirect/direct influences on humans. It is necessary to evaluate the biological characteristics, ecological influence, and harms of invasive species in preserved areas to establish a system to manage invasive species and plan the priority removal of species that are highly harmful and locally distributed. Manual removal and suppression of new biological organisms that cause problems is effective, but it takes cost, effort, and time. Therefore, we would need to research and develop the most efficient and effective techniques and measures to manage invasive species in our ecosystem.

• Korean journal of applied entomology
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• v.17 no.2 s.35
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• pp.99-103
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• 1978

Population density of the spiders (Micryphantidae and Lycosidae) in the two Paddy field at Suweon and Iri was studied following single root-zone application of carbofurans encapsulea and liquid-formulated and two to four paddy water applications of carbofuran to rice plants. In two field tests, the spider populations were reduced In all insecticide treatments. Among them, the reduction was greater in the capsule placement of carbofuran than in the liquid injection and broadcast of carbofuran. Their reduction was also enlarged with the increase of application rate of insecticide. The significant reduction of spider population in root-tone appliration of carbofuran was considered due to the food-chain toxicity on the spider-hopper system.

• Journal of Plant Biotechnology
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• v.34 no.2
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• pp.145-152
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• 2007

Lactoferrin is an 80-kDa iron-binding glycoprotein known to exert many biological activities, such as facilitating iron absorption and having antimicrobial and anti-inflammatory effects. Rice can be a useful target for edible food plants to introduce human lactoferrin, because it has lower allergenicity and is likely to be safer than microorganisms or transgenic animals. A cDNA fragment encoding human lactoferrin (HLF) driven by the maize polyubiquitin promoter, along with herbicide resistance gene (bar) driven by CaMV 35S promoter, was introduced into rice (Oryza sativa L. cv. Dong Jin) using the Agrobacterium -mediated transformation system. Putative transformants were initially selected on the medium containing bialaphos. The stable integration of the bar and HLF genes into transgenic rice plants was further confirmed through polymerase chain reaction (PCR) and Southern blot analyses. The expression of the full length HLF protein from various tissues such as grains and young leaves of transgenic rice was verified by Western blot analysis. Analysis of progeny also demonstrated that introduced genes were stably inherited to the next generation at the Mendelian fashion.

• Korean Journal of Ecology and Environment
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• v.37 no.3 s.108
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• pp.304-312
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• 2004

The transient dynamics of three-trophic populations (prey, predator, and super predator) using ratio-dependent models responding to environmental impacts is analyzed. Environmental factors were divided into two parts: periodic factor (e.g., temperature) and general noise. Periodic factor was addressed as a frequency and bias, while general noise was expressed as a Gaussian distribution. Temperature bias ${\varepsilon}$, temperature frequency ${\Omega}$, and Gaussian noise amplitude ${\`{O}}$ accordingly revealed diverse status of population dynamics in three-trophic food chain, including extinction of species. The model showed stable limit cycles and strange attractors in the long-time behavior depending upon various values of the parameters. The dynamic behavior of the system appeared to be sensitive to changes in environmental input. The parameters of environmental input play an important role in determining extinction time of super predator and predator populations.

• Journal of Korean Society on Water Environment
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• v.34 no.6
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• pp.579-590
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• 2018

The lakes' metabolism bears important information for the assessment of the carbon budget due to the accumulation or loss of carbon in the lake as well as the dynamics of the food webs through primary production. A lake-scale metabolism is evaluated by Gross Primary Production (GPP), Ecosystem Respiration (R), and Net Ecosystem Production (NEP), which is the difference between the first two values. Methods for estimating GPP and R are based on the levels carbon and oxygen. Estimation of carbon is expensive because of the use of radioactive materials which requires a high degree of proficiency. The purpose of this study was to estimate Lake Daecheong ecosystem metabolism using high frequency water temperature data and DO measurement sensor, widely utilized in the field of water quality monitoring, and to evaluate the possibility of using the application method. High frequency data was collected at intervals of 10 minutes from September to December 2017 by installing a thermistor chain and a DO sensor in downstream of Daechung Dam. The data was then used to estimate GPP, R and NEP using the R public program LakeMetabolizer, and other metabolism models (mle, ols, kalman, bookkeep). Calculations of gas exchange coefficient methods (cole, crusius, heiskanen, macIntyre, read, soloviev, vachon) were compared. According to the result, Lake Daecheong has some deviation based on the application method, but it was generally estimated that the NEP value is negative and acts as a source of atmospheric carbon in a heterotrophic system. Although the high frequency sensor data used in this study had negative and positive GPP and R values during the physical mixing process, they can be used to monitor real-time metabolic changes in the ecosystem if these problems are solved.

• Biomedical Science Letters
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• v.27 no.1
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• pp.35-43
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• 2021

Human Sapovirus (HuSaV) is one of the major causes of acute gastroenteritis in humans, and it is used as a molecular diagnostic technique based on polymerase chain reaction (PCR) from humans, food, shellfish, and aquatic environments. In this study, the HuSaV diagnosis technique was used in an aquatic environment where a number of PCR inhibitors are included and pathogens, such as viruses, are estimated to exist at low concentration levels. HuSaV-specific primers are improved to detect 38 strains registered in the National Center for Biotechnology Information (NCBI). The established optimal condition and the composition, including the RT-nested PCR primers and SL® Non-specific reaction inhibitor, were found to have 100 times higher sensitivity based on HuSaV plasmid than the previously reported methods (100 ag based on HuSaV plasmid 1 ng/μL). Through an artificial infection test, the developed method was able to detect at least 1 fg/μL of HuSaV plasmid contaminated with total nucleic acid extracted from groundwater. In addition, RT-nested PCR primer sets for HuSaV detection can react, and a positive control is developed to verify false positives. This study is expected to be used as a HuSaV monitoring method in the future and applied to the safety response to HuSaV from water environments.

• Nutrition Research and Practice
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• v.15 no.2
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• pp.160-172
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• 2021

BACKGROUND/OBJECTIVES: Nutritional status and food intake during pregnancy and lactation can affect fetal programming. In the current metabolic syndrome epidemic, high-fructose diets have been strongly implicated. This study investigated the effect of maternal high-fructose intake during pregnancy and lactation on the development of metabolic syndrome in adult offspring. SUBJECTS/METHODS: Drinking water with or without 20% fructose was administered to female C57BL/6J mice over the course of their pregnancy and lactation periods. After weaning, pups ate regular chow. Accu-Chek Performa was used to measure glucose levels, and a tail-cuff method was used to examine systolic blood pressure. Animals were sacrificed at 7 months, their livers were excised, and sections were stained with Oil Red O and hematoxylin and eosin (H&E) staining. Kidneys were collected for gene expression analysis using quantitative real-time Polymerase chain reaction. RESULTS: Adult offspring exposed to maternal high-fructose intake during pregnancy and lactation presented with heavier body weights, fattier livers, and broader areas under the curve in glucose tolerance test values than control offspring. Serum levels of alanine aminotransferase, aspartate aminotransferase, glucose, triglycerides, and total cholesterol and systolic blood pressure in the maternal high-fructose group were higher than that in controls. However, there were no significant differences in mRNA expressions of renin-angiotensin-aldosterone system genes and sodium transporter genes. CONCLUSIONS: These results suggest that maternal high-fructose intake during pregnancy and lactation induces metabolic syndrome with hyperglycemia, hypertension, and dyslipidemia in adult offspring.

• Journal of Environmental Impact Assessment
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• v.26 no.1
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• pp.27-43
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• 2017

The objectives of the study were to evaluate fish compositions, endangered species, community structure, physical habitat, and general water quality for a preliminary environmental impact assessment of Jeokbyeok River on the road construction between two regions. Total number of species and total number of individuals, based on CPUE, were 23 and 1186, respectively. The endangered species (I, II) as the legal protection species were Pseudopungtungia nigra (79 samples) Gobiobotia brevibarba) (5) Gobiobotia macrocephala (2), indicating a requiring of endangered species conservation. In the meantime, exotic species and ecological disturbing species such as Micropterus salmoides and Lepomis macrochirus, were not present, indicating a well conserved area. According to fish community analysis, values of species diversity index were high (range: 0.788 - 1.030), and the dominance index were low (range: 0.097 - 0.183), indicating that the fish community in this area was maintained well without high dominacne by specific species. Also, fish analysis on tolerance guilds and trophic guilds showed that the proportions of sensitive species were largely exceeded the proportions of the tolerant species, while the proportions of insectivore species were largely exceeded the proportions of the omnivore species. This outcome suggests that the ecosystem was well maintained in terms of tolerance and trophic compositions (food chain). Ecological health, based on the multi-metric fish model of Fish Assessment Integrity (FAI), reflected those fish conditions. In other words, values of FAI model averaged 82.4, which means a "good condition" in the criteria of ecological health by the Minstry of Environment, Korea. In addition, general water quality and physical habitat analyses showed that the system was in good condition. Under these conditions, if the road constructions between the two regions happen in the future, inorganic suspended solids may increase in the waterbody, and this may result in indirect or direct influences on the physical habitats and food chain as well as fish compositions, so the ecological protections and prevention strategy from the soil erosion are required in the system.