• 농촌지도와개발
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• 제22권1호
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• pp.31-41
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• 2015

Rural tourism village experience is proceeded quantitatively without distinct characteristic. This research aimed at analyzing the experience and utilizing in the establishment of differentiation and contents development. Type of experience activity was classified as 10 types in Level 1 and 0~4 types in Level 2. As the result of analyzing 3,007 experiences in 168 villages, types of experience activity implemented per 1 village was 17.9. Among them, ecological experience type appeared to be the most, and appeared in order of food, agriculture farming experience. In respect of agriculture farming experience, 'harvest and utilization' was analyzed to be the highest, and regarding rural farmhouse living experience displayed 'farmhouse living' experience the highest. Tradition courtesy experience displayed 'traditional culture' experience the highest, and rural food experience was analyzed to implement 'food making' experience the most. Ecological experience mainly consisted of 'hunting and collecting' and 'observation/learning', in case of play experience, 'traditional play' experience activity was analyzed to be performed the most. Considering utilization material, it appeared in order of 'rice', 'sweet potato', 'potato', 'corn', 'chili', 'agricultural implement', 'farmhouse', 'animal', 'culture', 'history', 'rice cake', 'alcoholic drink', 'tofu', 'kimchi', etc. The place of ecological experience was performed in the forest the most, and lots of experience was performed in stream, valley, and river. The researcher expects that characteristic experience activity will be developed based on this result, by avoiding doubleness of the experience activity among the regions and the villages.

• Nutrition Research and Practice
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• 제6권6호
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• pp.542-550
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• 2012

This pilot study was performed to produce data of the Children's Dietary Life Safety (CDLS) Index which is required by the Special Act on Safety Management of Children's Dietary Life and to evaluate the CDLS Index for 7 metropolitan cities and 9 provinces in Korea. To calculate the CDLS Index score, data regarding the evaluation indicators in the children's food safety domain and children's nutrition safety domain were collected from the local governments in 2009. For data regarding the indicators in the children's perception & practice domain, a survey was conducted on 2,400 5th grade children selected by stratified sampling in 16 local areas. Relative scores of indicators in each domain were calculated using the data provided by local governments and the survey, the weights are applied on relative scores, and then the CDLS Index scores of local governments were produced by adding scores of the 3 domains. The national average scores of the food safety domain, the nutrition safety domain and the perception and practice domain were 23.74 (14.67-26.50 on a 40-point scale), 16.65 (12.25-19.60 on a 40-point scale), and 14.88 (14.16-15.30 on a 20-point scale), respectively. The national average score of the CDLS Index which was produced by adding the scores of the three domains was 55.27 ranging 46.44-58.94 among local governments. The CDLS Index scores produced in this study may provide the motivation for comparing relative accomplishment and for actively achieving the goals through establishment of the target value by local governments. Also, it can be used as useful data for the establishment and improvement of children's dietary life safety policy at the national level.

• Journal of Nutrition and Health
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• 제44권6호
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• pp.527-536
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• 2011

This study was performed to analyze children's perceptions and practice levels according to gender and obesity status using a dietary life safety index. A national survey was conducted on fifth grade children (n = 2,400), who were selected using three-stage stratified cluster sampling from 16 provinces. The average height was 144.8 cm, and weight was 38.8 kg. The average body mass index was 18.4 kg/$m^2$ and underweight, overweight, and obese children were identified using the 2009 KHNANES cutoff values, which were 5.3%, 10%, and 5.9%, respectively. The perception and practice scores for hand-washing prior to eating were high and the score for willing to buy at a clean store was also high. However, students answered that the hygiene level of food stores near the school was poor. More students skipped breakfast than lunch or dinner. The frequency scores for fruit and vegetables were significantly higher for girls than those for boys. Students had a good understanding of nutrition labeling but did not frequently check the label. Seventy-five percent of the students tried to avoid high calorie foods with low nutritional value, but only 40% had the appropriate knowledge about high calorie foods with low nutritional value. Girls had better dietary life perception and practice levels than those of boys. No differences in perception or practice levels were observed based on obesity status. Nutrition education on the importance of eating breakfast and having accurate knowledge on nutrition labeling and high calorie foods with low nutritional value is needed. Behavior-centered education should be implemented to improve the perceptions and practice level of student's dietary life.

• Mycobiology
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• 제42권1호
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• pp.66-72
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• 2014

Roselle (Hibiscus sabdariffa L.) family Malvaceae is an important crop used in food, cosmetics and pharmaceutics industries. Roselle is cultivated mainly in Upper Egypt (Qena and Aswan governorates) producing 94% of total production. Root rot disease of roselle is one of the most important diseases that attack both seedlings and adult plants causing serious losses in crop productivity and quality. The main objective of the present study is to identify and characterize pathogens associated with root rot and wilt symptoms of roselle in Qena, Upper Egypt and evaluate their pathogenicity under greenhouse and field condition. Fusarium oxysporum, Macrophomina phaseolina, Fusarium solani, Fusarium equiseti and Fusarium semitectum were isolated from the natural root rot diseases in roselle. All isolated fungi were morphologically characterized and varied in their pathogenic potentialities. They could attack roselle plants causing damping-off and root rot/wilt diseases in different pathogenicity tests. The highest pathogenicity was caused by F. oxysporum and M. phaseolina followed by F. solani. The least pathogenic fungi were F. equiseti followed by F. semitectum. It obviously noted that Baladi roselle cultivar was more susceptible to infection with all tested fungi than Sobhia 17 under greenhouse and field conditions. This is the first report of fungal pathogens causing root rot and vascular wilt in roselle in Upper Egypt.

• Journal of Microbiology and Biotechnology
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• 제29권8호
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• pp.1255-1265
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• 2019

To investigate the diversity of gastrointestinal microflora and lignocellulose-degrading enzymes in wild Asian elephants, three of these animals living in the same group were selected for study from the Wild Elephant Valley in the Xishuangbanna Nature Reserve of Yunnan Province, China. Fresh fecal samples from the three wild Asian elephants were analyzed by metagenomic sequencing to study the diversity of their gastrointestinal microbes and cellulolytic enzymes. There were a high abundance of Firmicutes and a higher abundance of hemicellulose-degrading hydrolases than cellulose-degrading hydrolases in the wild Asian elephants. Furthermore, there were a high abundance and a rich diversity of carbohydrate active enzymes (CAZymes) obtained from the gene set annotation of the three samples, with the majority of them showing low identity with the CAZy database entry. About half of the CAZymes had no species source at the phylum or genus level. These indicated that the wild Asian elephants might possess greater ability to digest hemicellulose than cellulose to provide energy, and moreover, the gastrointestinal tracts of these pachyderms might be a potential source of novel efficient lignocellulose-degrading enzymes. Therefore, the exploitation and utilization of these enzyme resources could help us to alleviate the current energy crisis and ensure food security.

• Asian-Australasian Journal of Animal Sciences
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• 제26권2호
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• pp.253-259
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• 2013

Paddy rice is rarely used as a feed because of its high fiber content. In this study, two experiments were conducted to study the effects of supplementing an enzyme complex consisting of xylanase, beta-glucanase and cellulase, to paddy-based diets on the performance and nutrient digestibility in meat-type ducks. In the both experiments, meat-type ducks (Cherry Valley) were randomly assigned to four treatments. Treatment 1 was a basal diet of corn-soybean; treatment 2 was a basal diet of corn-paddy-soybean; treatment 3, had enzyme complex added to the corn-paddy-soybean basal diet at levels of 0.5 g/kg diet; and treatment 4, had enzyme complex added to the corn-paddy-soybean diet at levels of 1.0 g/kg diet. The results showed that the enzyme complex increased the ADG, and decreased the ADFI and F/G significantly (p<0.05) in the ducks, and the ADFI for the ducks fed the corn-paddy-soybean diet showed no difference compared to the ducks fed corn-soybean diets at all stages of the experiment (p<0.05). When corn was partially replaced by paddy, the digestibility of CP and NDF was decreased and increased, respectively (p<0.05), and the level of enzyme complex had a significant effect on both CP and NDF digestibility (p<0.05). As for the AME, addition of enzyme complex increased it significantly (p<0.05), but both diet types and levels of enzyme complex had no effect (p>0.05). The outcome of this research indicates that the application of enzyme complex made up of xylanase, beta-glucanase, and cellulase, in the corn-paddy-soybean diet, can improve performance and nutrition digestibility in meat-type ducks.

• Journal of Nutrition and Health
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• 제53권6호
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• pp.563-569
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• 2020

Purpose: The vascular smooth muscle cells (VSMCs) in mature animals have implicated to play a major role in the progression of cardiovascular diseases such as atherosclerosis. This study aimed at optimizing the protocol in culturing primary VSMCs (pVSMCs) from rat thoracic aorta and investigating the effect of cellular zinc (Zn) deficiency on cell proliferation of the isolated pVSMCs. Methods: The thoracic aorta from 7-month-old Sprague Dawley rats was isolated, minced and digested by the enzymatic process of collagenase I and elastase, and then inoculated with the culture Dulbecco Modified Eagle Medium (DMEM) at 37℃ in an incubator. The primary cell culture morphology was observed using phase-contrast microscopy and cellular Zn was depleted using Chelex-100 resin (extracellular zinc depletion only) or 3 µM N,N,N',N'-tetrakis(2-pyridinylmethyl)-1,2-ethanediamine (TPEN) (extracellular and intracellular zinc depletion). Western blot analysis was used for the detection of SM22α and calponin as smooth muscle cell marker proteins and von Willebrand factor as endothelial cell marker protein to detect the culture purity. Cell proliferation by Zn depletion (1 day) was measured by MTT assay. Results: A primary culture protocol for pVSMCs from rat thoracic aorta was developed and optimized. Isolated cultures exhibited hill and valley morphology as the major characteristics of pVSMCs and expressed the smooth muscle cell protein markers, SM22α and calponin, while the endothelial marker von Willebrand factor was hardly detected. Zn deprivation for 1 day culture decreased rat primary vascular smooth muscle cell proliferation and this pattern was more prominent under severe Zn depletion (3 µM TPEN), while less prominent under mild Zn depletion (Chelexing). Conclusion: Our results suggest that cellular Zn deprivation decreased pVSMC proliferation and this may be involved in phenotypic modulation of pVSMC in the aorta.

• 한국농촌건축학회논문집
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• 제26권2호
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• pp.37-46
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• 2024

The city is developing into a smart city. Smart villages and smart farms are developing in rural areas. Architectural technology needs synergy with smart cities, smart villages, and smart factories (intelligent factories) to help architectural experts understand smart farms and build facilities and equipment. Smart farms require design and construction technology with architectural structure and function. The purpose of this study was to investigate the current status and cases of smart farms in Korea and to investigate cases abroad. The conclusion is as follows. ① Smart farms are developing rapidly. The Korean government is expanding smart farms by utilizing ICT technology and infrastructure. ② 'Smart Farm Innovation Valley', which has been promoted since 2018, is a cutting-edge convergence cluster industrial complex that integrates production, education, and research functions such as start-ups and technological innovation. ③ In domestic cases, smart farms are operated in subway stations, buildings, supermarkets, and restaurants. ④ In the Japanese case, a dome-type smart farm was being operated. It utilized factory wastewater, waste heat, renewable energy, and used new materials. Otemachi Ranch raised livestock and provided a lounge on the 13th floor of the building. ⑤ In the cases of Korea and Japan, the smart farm technology is very similar. As stated earlier, since the food culture and agricultural technology of both countries are similar, we hope to promote the development of smart farms that can reduce concerns about future food by communicating and sharing mutual technologies.

• Journal of Nutrition and Health
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• 제44권1호
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• pp.49-60
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• 2011

본 연구는 특별법에 의한 어린이식생활 안전지수를 개발하여 지자체별로 비교 평가하기 위한 안전지수의 영역을 구성하고 영역별로 세부지표를 도출하는 목적으로 수행되었다.식생활 안전지수 평가지표 개발을 위해 선행연구에 대한 검토 및 식생활 안전지수 평가지표 전문가 자문회의를 실시하여 평가지표를 개발하였으며, AHP 기법과 델파이 조사 기법을 활용하여 각 평가지표에 대한 가중치를 부여하였다. 연구의 결과는 다음과 같다. 어린이 식생활 안전지수 평가지표는 수차에 걸친 식품, 영양 관련 분야 전문가 회의를 거쳐 최종적으로 정책지표 3개, 전략지표 9개, 대표 평가지표 11개 및 세부 평가지표 20개를 선정하였다. 선정된 세부 평가지표에 대해 AHP 기법과 델파이 기법을 활용하여 각 세부지표별 가중치를 선정하였다. 정책지표별 가중치는 식생활 안전 0.4, 식생활 영양 0.4, 식생활 인지 실천수준 0.2이었고, 전략지표별 가중치는 식생활 안전관리 지원수준 0.12, 기호식품 안전관리수준 0.19, 단체급식 안전관리수준 0.19, 결식 및 비만관리 수준 0.13, 기호식품 영양관리수준 0.11, 단체급식 영양관리수준 0.16, 식생활 안전 및 영양제도 인지수준 0.04, 식생활 안전관리 인지 실천 수준 0.07, 식생활 영양관리 인지 실천 수준 0.09이었다.

• 한국환경생태학회지
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• 제31권4호
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• pp.397-408
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• 2017

본 연구의 목적은 국내산 남생이와 외래종 붉은귀거북의 행동권 및 서식지 이용 패턴을 확인하고 종간 상호 경쟁관계를 분석하는 것이다. 본 연구는 2010년 8월 2일부터 2011년 1월 30일까지 경기도 과천시 청계산 계곡 상류지역에 위치한 정수역인 금붕어광장 연못에서 수행되었다. 서울동물원에서 인공증식한 남생이 3개체와 국내의 연못과 저수지에 서식하고 있던 붉은귀거북 3개체를 포획하여 위치추적발신기를 부착한 후 방사하여 모니터링을 수행하였다. 방사된 개체들의 행동권과, 서식지 이용반경을 측정하였고, 금붕어광장 연못의 온 습도 및 수온, 지온 등 환경적인 변화를 측정하였다. 본 연구 결과는 남생이와 붉은귀거북은 제한된 일광욕 장소, 먹이자원 이용, 동면지 등 여러 측면에서 중복된 생태적 지위를 가지고 있어서 상호 경쟁이 큰 것으로 분석되었다. 비교적 몸집이 작은 남생이가 몸집이 큰 붉은귀거북에게 경쟁에서 밀려나는 것을 확인할 수 있었다. 남생이가 서식하고 있는 원서식지에 붉은귀거북의 이입을 통한 종간, 개체간의 행동권과 먹이경쟁, 서식지 이용 등에 대한 연구가 추가적으로 필요할 것으로 사료되며, 추후 남생이 복원사업의 기초자료가 될 것이다.