• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2002.08a
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• pp.13-31
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• 2002

쌀은 중국에서 중요한 식량작물로 제9차 5개년 계획(1966-2000년) 동안 재배면적 31.4백만 ha이며 생산량은 단위 ha당 6,303kg으로 198백만톤에 이르며 이는 재배면적으로는 식량작물의 27.7%, 그리고 생산량으로는 전체식량작물의 40%를 각각 점하고 있다. 이러한 재배면적과 생산량은 각각 세계전체면적과 생산량의 20.7%와 33.7%를 차지하는 많은 량이다. 중국의 남부지역은 전지역의 73.5%가 이모작으로 재배되며 주품종은 Indica이다. 중국의 중부지역은 이모작과 일모작의 재배형태가 2:3으로 공존하고 있으며 양쯔강 이북은 주로 일모작의 형태이다. 중국의 쌀 재배면적은 1960년대 이후 점차 증가하다가 1980년대 후반으로 정체되었다가 최근 90년대 말에 이르러서는 재배면적의 감소가 가속화되고 있으나 단보당 생산량은 꾸준히 증가하고 있다. 2001년 중국의 쌀소비량은 138백만톤으로 이의 85.2%는 식량용으로, 5.8%는 사료용으로, 1.3%는 가공용, 1.5-2.0%는 수출용으로 그리고 1.2%는 종자용으로 소비되었다. WTO체제에 들어서도 중국의 쌀 생산에는 크게 영향을 받지않을 것으로 여겨지는데 그 이유로는 충분한 생산능려과 자급률, 쌀의 낮은(4%) 국제교역비율, 총생산량에 대한 낮은 쿼터비율 등을 들 수 있다. 그러나 WTO체제 가입에 따른 압력 또한 존재하는 것이 사실인데 그것은 낮은 품질, 국제가격보다 높은 국내가격 등을 들 수 있다. 향후 중국 쌀의 발전적 전략들로는 쌀의 안정적 발전을 지속하는 일, 쌀 재배구조 조정과 함께 높은 미질을 가지는 품종육종, 기계화를 비롯한 경작기술의 발달, 쌀과 부산물 가공기술의 개발연구, 특징 기능을 함유하는 유전공학적 기술의 적용, 토지와 도시화 그리고 식량순환에 시스템의 개혁 등 과학기술을 고양하는 일 등을 들 수 있다.TEX>$\times$10cm의 소식일수록 짧아서 재식주수와 경장은 정의상관이 인정되었다. 9 경직경은 30$\times$10cm, 40$\times$10cm의 소식일수록 크고 20$\times$10cm의 밀식일수록 작았다 10. 수량구성요소인 주근장과 수량인 건근중은 30$\times$10cm, 40$\times$10cm의 재식주수가 적을수록 높아서 재식주수와는 부의 상관이 인정되었다. 11 이상과 같은 결과로 보아 경직경이 크고 주근장이 길어서 10a당 건근중이 많은 30$\times$10cm(33주/$m^2$)가 알맞은 재식거리였다.에 대한 인식을 새롭게 하고 농약취급시의 건강장해예방행동을 촉구하는 등의 효과도 높은 것으로 예방의학적인 유용성이 크다고 볼 수 있다. 미침을 알 수 있었다. 대두 단백질로 코팅된 golden delicious는 상온에서60일 동안 보관하였을 경우, 사과표피의 색도 변화를 현저히 지연시킴을 확인하였다. 또한 control과 비교하여 성공적으로 사과에 코팅하였으며, 상온에서 보관하여을 때 사과의 품질을 30일 이상 연장하는 효과를 관찰하였다. 이들 결과로부터 대두단백질 필름이 과일 등의 포장제로서 이용할 가능성을 확인하였다.로 [-wh] 겹의문사는 복수 의미를 지닐 수 없 다. 그러면 단수 의미는 어떻게 생성되는가\ulcorner 본 논문에서는 표면적 형태에도 불구하고 [-wh]의미의 겹의문사는 병렬적 관계의 합성어가 아니라 내부구조를 지니지 않은 단순한 단어(minimal $X^{0}$ elements)로 가정한다. 즉, [+wh] 의미의 겹의문사는 동일한 구성요 소를 지닌 병렬적 합성어([$

• Journal of Life Science
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• v.21 no.1
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• pp.68-73
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• 2011

$\beta$-1,3-glucanase from Pyrococcus furiosus was applied for the saccharification of laminarin, which is a major oligo-saccharide component of brown algae, and the reaction mixture produced from laminarin was utilized as a substrate for alcohol fermentation using yeast. To prepare the recombinant $\beta$-1,3-glucanase, a $\beta$-1,3-glucanase gene was overexpressed in Escherichia coli and purified. Laminarin was degraded to an oligo- and mono-saccharide, such as glucose, after reaction with the purified recombinant $\beta$-1,3-glucanase, and the products after enzymatic treatment were confirmed by TLC and HPLC analysis. Decomposed laminarin after enzyme reaction was only added to the medium as a C-source for yeast alcohol production reaction. 0.3% alcohol production was detected from the cultured broth by gas chromatography after 48 hr of incubation. Further evaluation for optimal conditions of saccharification and alcohol fermentation can be suggested, as well as the possibility of using this enzymatic method to produce ethanol using laminarin.

• Journal of Life Science
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• v.27 no.9
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• pp.1003-1010
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• 2017

An open reading frame coding for mannanase predicted from the partial genomic sequence of Paenibacillus woosongensis was cloned into Escherichia coli by polymerase chain reaction amplification, and completely sequenced. This mannanase gene, designated man26AT, consisted of 3,162 nucleotides encoding a polypeptide of 1,053 amino acid residues. Based on the deduced amino acid sequence, Man26AT was identified as a modular enzyme, which included a catalytic domain belonging to the glycosyl hydrolase family 26 and two carbohydrate-binding modules, CBM27 and CBM11. The amino acid sequence of Man26AT was homologous to that of several putative mannanases, with identity of 81% for P. ihumii and identity of less than 57% for other strains of Paenibacillus. A cell-free extract of recombinant E. coli carrying the man26AT gene showed maximal mannanase activity at $55^{\circ}C$ and pH 5.5. The enzyme retained above 80% of maximal activity after preincubation for 1 h at $50^{\circ}C$. Man26AT was comparably active on locust bean gum (LBG), galactomanan, and kojac glucomannan, whereas it did not exhibit activity on carboxymethylcellulose, xylan, or para-nitrophenyl-${\beta}$-mannopyranoside. The common end products liberated from mannooligosaccharides, including mannotriose, mannotetraose, mannopentaose, and mannohexaose, or LBG by Man26AT were mannose, mannobiose, and mannotriose. Mannooligosacchrides larger than mannotriose were found in enzymatic hydrolyzates of LBG and guar gum, respectively. However, Man26AT was unable to hydrolyze mannobiose. Man26AT was intracellularly degraded into at least three active proteins with different molecular masses by zymogram.

• Journal of Korean Home Economics Education Association
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• v.20 no.4
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• pp.91-105
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• 2008

The purpose of this study was to conduct a questionnaire survey for total 503 high school female students in Wonju area, Kangwon province, and thereby determine any potential effectiveness of their trials to reduce weight in their satisfaction with their own figure, daily living practices related to weight control, nutrition knowledge and dietary behaviors. Out of all respondents, it was found that 284 girl students(58.4%) ever tried to reduce weight, and both trial group(trying to reduce weight) and no-trial group were much interested in weight control(94.7% vs. 60.7%). But 85.2% of trial group and 50.3% of no-trial group were dissatisfied at their figure. It was found that the percentage of trial group practicing regular exercise was higher than that of no-trial group doing so(21.8% vs. 5.5%). Subjects scored 14.3 out of 20 on a nutrition knowledge scale, but trial group scored significantly higher points(14.5) than no-trial group(13.9)(p<0.01). In particular, it was notable that trial group showed higher rate of correct answer in certain items such as balanced diet and knowledge about weight control. It was found that our all respondents showed moderate dietary behaviors, which is demonstrated by their mean score of 31.3 in dietary behaviors(possible score: $15{\sim}45$). Notably, it was found that there were problems exposed in their dietary velocity, intake of various diets and intake frequency for each food group(dairy products, marine plants and vegetative oil and fat). And it was found that the percentage of trial group taking diet with vegetative oil was higher than that of no-trial group.

• Journal of Society of Preventive Korean Medicine
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• v.12 no.2
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• pp.13-25
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• 2008

The purpose of this study was to investigate extract from mixed culture with Trichloloma matsutake mycelium in oriental medicine and cereal medium(OCM) to develop new material for pharmaceutical products and medicinal food for diabetes mellitus. To evaluate of hypoglycemic activity of OCM extracts, we examined the inhibitory activity of ${\alpha}$-glucosidasein OCM, blood glucose level and liver function of streptozotocin(STZ) induced diabetic rat. Experimental group was divided into 6 groups: first, it was divided into normal control group(hereafter NC group) and diabetes-induced group, and diabetes-induced group was subdivided into diabetic control group(DC group), treated by hot water extracts group(HE), ultra sonic waves, micro waves, and micro bubble extracts g roup(UE), crude polysaccharide of HE group (HEE) and crude polysaccharide of UE group(UEE) at a dose of 300mg/kg/body weight, respectively. In diabetic-induced groups, after streptozotocin was melted in 0.01M citrate buffer at 50mg/kg/body weight, when the non-fasting blood glucose levelwas 300 mg/dl or more in blood collected from the tail vein, it was regarded as diabetic induction and then such diabetic-induced experimental animals were used in this experiment. At the end of the experiment, blood glucose level increased by 4.19% in DC group but significantly decreased by 32.34%, 19.19%, 17.81% and 17.64%, respectively in UEEE, UE, HE, and HEE groups. In the cases of AST, ALT, and ALP, the experiment group treated with extracts showed significantly lowerblood glucose level than DC group. The levels of BUN and uric acid were found to be lower in the UMPM extract group(UE) than HW extract group(HE), which implies that herb medicine medium extracts in which Tricholoma matsutake mycelia were cultured are effective in reducing impaired liver function as well as high blood glucose level caused by diabetes. In addition, the administration of low temperature UMPM extracts was found to produce better results than that of high temperature hot water extracts. In this regard, it is expected that extracts from herb medicine obtained by cultivating Tricholoma matsutake mycelia will be widely used as new ingredients for foods and medicines for prevention and treatment of diabetes.

• Archives of design research
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• v.17 no.4
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• pp.97-106
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• 2004

Oukjuk becomes to known to the people and valuates due to the importance of the effect of the medicine. Known as taoist hermit plant, Oukjuk tea has called as a Solomon's Seal Tea in the western countries. Dissimilarly from other herb teas, it contains a high percentage of minerals. Because of the utility value of this, people used to use Oukjuk as an oriental medical treatment for various kinds of the condition of illness as for nutritive food or tonic material. According to the records, a sage of old or people seeking after truth had enjoyed the tea very much. In spite of these all effects, public has only a superficial understanding of the matter, Comprehending the problems, this study analyses ways and means of the functional packaging for the Solomon's Seal tea. In this research, the most problems came out with the quality of the products and the level of their packaging which is not variously in forms and not properly developed in materials and designs. As a conclusion the paper pointed out the importance of keeping the traditional high quality in product and having an appeal to packaging for customer's needs.

• Korean Journal of Community Nutrition
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• v.13 no.4
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• pp.463-475
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• 2008

This study investigated intake patterns of health functional foods(HFF) in elementary school children and requirements of their parents in the Daejeon area using a questionnaire by 432 parents. Participating parents were composed of female, 83.6% and male, 16.4%. Most of the parents were in their 40's(53.0%) and 30's(45.8%). 47.9% of the children were male and 52.1% were female. The school year of the children was distributed evenly, ranging from the first year to the sixth year. The intake rate of HFF for parents appeared to be 65.3% and for children it appeared to be 63.2%. The primary reason of using HFF to their child was 'For health promotion'(54.4%); the largest reason of non-using HFF was 'Do not feel necessity because is healthy'(48.8%). In regards to purchase place, 'Drugstore' was most selected by 26.1%, while the largest amount of purchase motive was 'Decided by oneself for children's health'(37.7%). The average expense per month was, 'Less than 50 thousand portion'(20.3%). The intake period, 'less than 6 months' was won' for 54.9%. For intake items, 'Vitamin supplementation products' was the largest portion'(20.3%) was indicated. The intake period, less than 6 months' was the most selected by 51.3%. Most people(66.7%) selected '1 kind' of intake item. As for intake effect, 45.4% claimed 'A little help'. In regard to experiencing side effects, 'Is not' was 92.7%, however, diarrhea, nettle rash, nausea etc. were a little. The biggest problem of HFF was 'Falsehood/exaggerative advertisement' as identified by 53.1%. For improvement of the HFF system was, 'Verify by more strict formality' as selected by 55.8%. In regards to experience of nutrition education on HFF, 'Is not' was most selected by 51.0%. The most desirous form of nutrition education was, 'Simple paper material'(31.3%). The most desirous content of nutrition education was, 'nutritional management of classified by life cycle'(37.2%). Therefore, HFF must be used properly to promote the health and growth development of children by acquiring scientific and reasonable information about the ability and usage of the food.

• Microbiology and Biotechnology Letters
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• v.48 no.2
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• pp.158-166
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• 2020

A gene coding for the xylanase was cloned from Paenibacillus woosongensis, followed by determination of its complete nucleotide sequence. This xylanase gene, designated as xyn10A, consists of 1,446 nucleotides encoding a polypeptide of 481 amino acid residues. Based on the deduced amino acid sequence, Xyn10A was identified to be a modular enzyme composed of a catalytic domain highly homologous to the glycosyl hydrolase family 10 xylanase and a putative carbohydrate-binding module (CBM) in the C-terminus. By using DEAE-sepharose and phenyl-sepharose column chromatography, Xyn10A was purified from the cellfree extract of recombinant Escherichia coli carrying a P. woosongensis xyn10A gene. The N-terminal amino acid sequence of the purified Xyn10A was identified to exactly match the sequence immediately following the signal peptide predicted by the Signal5.0 server. The purified Xyn10A was a truncated protein of 33 kDa, suggesting the deletion of CBM in the C-terminus by intracellular hydrolysis. The purified enzyme had an optimum pH and temperature of 6.0 and 55-60℃, respectively, with the kinetic parameters V_max and K_m of 298.8 U/mg and 2.47 mg/ml, respectively, for oat spelt xylan. The enzyme was more active on arabinoxylan than on oat spelt xylan and birchood xylan with low activity for p-nitrophenyl-β-xylopyranoside. Xylanase activity was significantly inhibited by 5 mM Cu²⁺, Mn²⁺, and SDS, and was noticeably enhanced by K⁺, Ni²⁺, and Ca²⁺. The enzyme could hydrolyze xylooligosaccharides larger than xylobiose. The predominant products resulting from xylooligosaccharide hydrolysis were xylobiose and xylose.

• Journal of Dairy Science and Biotechnology
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• v.35 no.4
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• pp.255-261
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• 2017

The purpose of this study was to investigate the probiotic properties and antioxidant capacity of lactic acid bacteria isolated from Vietnamese feces and the Korean traditional food kimchi. Six isolated strains were identified as Lactobacillus sp. by 16S rRNA sequencing. All strains showed good resistance to low pH (1.5, 2.0, and 3.0) and 0.3% oxgall bile acids. Culture filtrates from the six strains showed various antioxidant effects, including DPPH, ABTS, reducing power, and metal chelating ($Fe^{2+}$) activities. Two of the six Lactobacillus strains showed potential probiotic activity. Heat resistance and adhesion assays were conducted by mixing the selected strains, Lactobacillus acidophilus V4, Lactobacillus plantarum V7, and Lactobacillus paracasei DK121 isolated from kimchi. The results showed that the heat resistance of these strains was similar to that of a commercial strain, L. plantarum LP. In addition, a mucin attachment assay using the mixture of selected strains (V4, V7, and DK121) showed high binding activity to the mucous layer. In conclusion, a mixture of V4, V7, and DK121 shows promising probiotic activity and may be useful for the development of health-related products.

• Applied Biological Chemistry
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• v.49 no.3
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• pp.202-208
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• 2006

Since abnormal fermentation and short storage duration of oriental melon are the main problems causing loss in commercial value, it is necessary to develop a food processing method using uncommercial melon. In this study, we suggested the effective pretreatment and storage conditions of melon as the material for alcoholic beverage production. Abnormally fermented melon had smaller carbohydrate and larger moisture content than normal one, indicating that carbohydrate in normal melon was probably converted to fermented products during fermentation. The sugar content of oriental melon was increased after fruiting and the highest value $(12.4^{\circ}Brix)$ was found at 5 weeks of storage. The maximum storage duration of normal and fermented oriental melons were 25 and 7 days at $4^{\circ}C$, and 8 and 4 days at room temperature, respectively. The oriental melon for fermentation-use could be conserved after slicing for 30 days at $4^{\circ}C$ with the addition of 1.5% citric acid and for one year at $-20^{\circ}C$ with the plastic film sealing, respectively.