• Nutrition Research and Practice
• /
• 제4권4호
• /
• pp.323-331
• /
• 2010

The aim of this study was to analyse effects that the degree of depression have on the life style variables, nutrient intake, iron indices and pregnancy outcome. Subjects were 114 pregnant women who were receiving prenatal care at a hospital in Seoul. We collected data for general characteristics and lifestyle variables from general survey instrument and for depression score from the questionnaire on depression. Dietary intakes of subjects were estimated by 24 hour dietary recall method. Also we analysed iron indices and pregnancy outcomes. We classified subjects by 10 point, which was the average depression score, into two groups [Low depression score group (LS) : High depression score group (HS)]. As to the intakes of total calcium, plant-calcium, plant-iron, potassium, total folate and dietary folate, LS group was far higher than HS group (P < 0.05, P < 0.05, P < 0.01, P < 0.001, P < 0.05, and P < 0.01, respectively). As to pre-pregnancy alcohol drinking, LS group had 41.9% in non-drinker, which was far higher than 28% in HS group in non-drinker (P < 0.05). As for drinking coffee during pre-pregnancy, pregnant women who don’ drink coffee in LS group took 43.6%, which was higher than 38% in HS group (P < 0.01). Regarding delivery type, the cesarean section in LS group (18%) was significantly lower than that in HS group (45%) (P < 0.01). Bivariate analysis showed that birth weight was significantly associated with the gestational age (P < 0.01). The pregnant women with higher depression score tended to have undesirable life habit, which might affect negative pregnancy outcomes. A better understanding of how depression and intake of nutrients work together to modulate behavior will be benefit nutritional research.

• Journal of Community Nutrition
• /
• 제3권2호
• /
• pp.87-95
• /
• 2001

This study was conducted to investigate nutritional knowledge, dietary attitudes, and dietary behaviors of adult women, and to examine if these characteristics were different by acne status. Subjects were 106 adult women residing in Seoul recruited from clients and employers at skin care centers, and housewives from apartment complexes. Surrey instrument was adapted or modified based on literature review and dietary intakes were assessed using 24-hour recalls for two days and CAN-pro. All data was statistically analyzed using x$^2$test and ANOVA. When examined by acne status, 41.5% of subjects were categorized into acne group, 20.8% were as ex-acne group, and 37.7% as no-acne group. Parental experience of acne was significantly related to acne status(p〈 0.01). Education, employmental status, and smoking or drinking status were not related to acne status. Subjects had a moderate level of nutritional knowledge(72.7 point) and the nutritional knowledge store was not significantly different by acne status. When examined by individual items, the groups showed significant difference on the items regarding vitamin C and necessity of carbohydrates(p 〈 0.05). Subjects showed favorable dietary attitudes. Although the acne group showed more favorable attitudes on the importance of nutrition on acne, the overall dietary attitudes of the acne group were not significantly different from the ex-acne group or no-acne group. Similarly only small differences were noticed in dietary behaviors or nutrient intakes by acne status. Cholesterol consumption was hitgher in the no-acne group than in the acne group or ex-acne group(p 〈 0.01). The intakes of energy, iron, and calcium was much below the RDA in three groups. Although there were not many significant differences in nutritional knowledge, dietary attitudes, and dietary behaviors by acne status, this study provided some baseline information regarding study variables by acne status.

• 한국축산식품학회지
• /
• 제31권1호
• /
• pp.27-31
• /
• 2011

To differentiate between frozen-thawed and fresh broiler breast fillets, different methods such as optical microscopy and measurement of drip loss, pH, torrymeter and K-value were performed. A total of 10 samples of fresh and frozen-thawed breast fillets were stored in a refrigerator ($4^{\circ}C$) for 5 d. Optical microscopy of the frozen-thawed breast fillets found structural changes caused by ice crystals, which may have significantly increased drip loss compared to fresh breast fillet. The pH and K-value could not be distinguished between the two breast fillets during storage. However, the torrymeter values of the fresh and frozen-thawed breast fillets were significantly different (p<0.05). The results indicate that both optical microscopy and torrymeter measurement can be effective methods for differentiating between fresh and frozen-thawed breast fillets. However, optical microscopy may be difficult to implement in the marketplace since it requires much time and effort. Thus, the determination of the torrymeter value is the easiest and most rapid instrumental method among those tested for the differentiation of frozen-thawed chicken breast fillet from fresh one.

• 한국식품위생안전성학회지
• /
• 제24권2호
• /
• pp.148-153
• /
• 2009

본 연구에서는 심혈관계 질환의 표지인자인 콜레스테롤의 농도를 새롭게 개발한 형광 크로마토그라피 방법에 의하여 측정 하고자 시도하였다. 개발된 측정 시스템은 크로마토그라피 스트립이 들어 있는 카트리지, 발색제 AEC, 콜레스테롤 옥사데이즈 (Cholesterol Oxidase, CO), 콜레스테롤 에스트라아제(Cholesterol Esterase, CE), 호스래 디쉬페록시다아제 (Horseradish peroxidase, HRP)를 포함하는 효소혼합 수용액, 그리고 형광 판독기로 구성되어 었다. 콜레스테롤 농도 변화에 따른 형광 세기 변화로 얻은 상관계수 r= 0.968로 측정 가능한 농도 범위에서 신뢰할 만한 직선성 관계를 제시하여 주었다. 회복률과 Hitachi 747 검사기기와 비교 테스트에서는 각각 106.5-94%와 상관관계 r = 0.939 (p<0.001)로 비교적 높은 유의성을 보여주었다. 따라서 본 연구에서 개발된 새로운 콜레스테롤 농도 측정법은 빠른 반응 시간 (8분)과 적은 시료량 ($5\;{\mu}l$)을 사용하기에 시료를 실험실로 운반할 필요없이 클리닉에서 측정 가능한 준현장검사 플랫폼형으로 개발되었으며 기존의 고가의 자동화 장비를 사용하는 생화학적 진단방식 대체용으로의 응용이 기대된다.

• 한국축산식품학회:학술대회논문집
• /
• 한국축산식품학회 2005년도 제36차 추계 학술발표대회
• /
• pp.196-203
• /
• 2005

축산식품 고기내의 잔류항생물질을 신속하고 간편하고 정확하게 분석하기 위한 시험법 개발 을 목적으로 하였다. 축산식품내의 일반적인 잔류항생물질에 대한 지금까지의 분석법으로는 Bioassay법, TLC법, ELISA법, GC법 및 HPLC법 등이 있지만 Streptomycin/Dihydro streptomycin, Neomycin에 대한 HPLC법은 거의 확립되어 있지 않은 실정이다. 우리나라의 공인 검사법으로는 Bioassay법 및 HPLC법 등이 있지만 그러나 지금까지의 방법으로는 검출감도가 낮은 것이 큰 문제점으로 되어 왔다. 본 연구에서는 DST에 대한 HPLC법에 대한 보고한 P. Edder 방법 중에 clean-up과정 및 이동상 조건을 대폭 수정하여 DST의 분리 및 검출감도를 낮추려고 시도하였다. 본 연구에 사용된 유도체화 장치 Post-Column Derivatization Instrument PCX 5100 (Pickering Laboratories, Inc.)의 컬럼온도는 $40^{\circ}C$, 오븐온도 $55^{\circ}C$, reagent 유속 0.6ml/min mobile phase 유속 0.8ml/min으로 검출기는 형광검출기를 이용하여 DST 검출에 대해 만족할 만한 결과를 얻었다. 이때의 분석소요시간은 약 15분이었다. 표준시료 DST의 검량선은 넓은 농도범위(0.02${\sim}$1.0ppm)에서 양호한 직선성을 나타냈다. 본 시험법에 의한 검출한계는 limit of detection (LOD)은 0.02ppm이었으며, 적어도 고기에서의 MRL이 0.6ppm임을 감안하면 DST를 정량적으로 정도 좋게 측정할 수 있다는 것을 확인했다. 상기의 조건하에서 실제시료인 고기에 표준 DST를 1ppm을 spiking한 후 SPE상에서 SCX(Strong cation exchange column)을 통한 clean-up과정을 거친 후의 DST의 limit of quantification(LOQ)는 약 0.47ppm이었으며, 이에 대한 회수율은 97.7%(n= 8)를 나타냈다. 실제 codex에서 권장한 고기의 MRL이 0.6ppm인 점을 감안하면 codex 권고치에 도달할 수 있는 것으로 판단되었다. 따라서 본 연구에서 개발된 시험법은 지금까지 국내적으로 DST에 대한 시험법이 확립되어 있지 않은 것으로 이와 아울러 간편한 parallux와 병용해 DST에 대한 정량 및 정성 분석을 유도체화 장치 및 형광검출기를 이용해 잔류항생물질 DST에 대한 분석시험법의 개발이 가능하다고 여겨진다.

• 한국축산식품학회:학술대회논문집
• /
• 한국축산식품학회 2005년도 제36차 추계 학술발표대회
• /
• pp.310-316
• /
• 2005

축산식품(우유)내의 잔류항생물질을 신속하고 간편하고 정확하게 분석하기 위한 시험법 개발을 목적으로 하였다. 축산식품내의 일반적인 잔류항생물질에 대한 지금까지의 분석법으로는 Bioassay법, TLC법, ELISA법, GC법 및 HPLC법 등이 있지만 Streptomycin/dihydrostreptomycin, neomycin에 대한 HPLC법은 거의 확립되어 있지 않은 실정이다. 우리나라의 공인 검사법으로는 Bioassay법 및 HPLC법등이 있지만 그러나 지금까지의 방법으로는 검출감도가 낮은 것이 큰 문제점으로 되어 왔다. 본 연구에서는 STP에 대한 HPLC법에 대한 보고한 Edder 방법 중에 clean-up 과정 및 이동상 조건을 대폭 수정하여 STP의 분리 및 검출감도를 낮추려고 시도하였다. 본 연구에 사용된 유도체화 장치 Post-Column Derivatization Instrument PCX 5100 (Pickering La-boratories, Inc.)의 컬럼 온도는 $40^{\circ}C$, 오븐온도 $55^{\circ}C$, 유도체화 용매 유속 0.6ml/min 이동상 유속 0.8ml/min으로 검출기는 형광검출기를 이용하여 STP 검출에 대해 만족할 만한 결과를 얻었다. 이때의 분석소요시간은 약 15분이었다. 표준시료 STP의 검량선은 넓은 농도범위(0.02${\sim}$1.0ppm)에서 양호한 직선성을 나타냈다. 본 시험법에 의한 검출한계는 limit of detection(LOD)은 0.02ppm이었으며, 적어도 우유에서의 MRL이 0.6ppm임을 감안하면 STP를 정량적으로 정도 좋게 측정할 수 있다는 것을 확인했다. 상기의 조건하에서 실제시료인 우유에 표준 STP를 0.5ppm을 spiking한 후 SPE상에서 SCX(Strong cation exchange column)을 통한 clean-up과정을 거친 후의 STP의 limit of quantification(LOQ)는 약 0.44ppm이었으며, 이에 대한 회수율은 89.7${\pm}$2.3%(n=6)를 나타냈다. 실제 CODEX에서 권장한 우유의 MRL이 0.6ppm인 점을 감안하면 CODEX권고치에 도달할 수 있는 것으로 판단되었다. 따라서 본 연구에서 개발 된 시험법은 지금까지 국내적으로 STP에 대한 시험법이 확립되어 있지 않은 것으로 이와 아울러 간편한 parallux와 병용해 STP에 대한 정량 및 정성 분석을 유도체화 장치 및 형광검출기를 이용해 잔류항생물질 STP에 대한 분석시험법을 개발하였다.

• 한국환경교육학회지:환경교육
• /
• 제23권1호
• /
• pp.99-111
• /
• 2010

The purpose of this study was to investigate high school students' perceptions of biotechnology. Participants in this study were 9th and 10th grade students who were enrolled in high schools in Gyeonggi Province. The survey instrument used in this study was a 26-item questionnaire that was designed to measure students' perceptions regarding biotechnology. The study revealed that students' perceptions were positive toward the use of biotechnology on biological objects such as plant, grain and microbes. However, their perceptions were negative toward the use of biotechnology on humans and animals. Male students' perceptions were more positive than female students and there were significant differences between male and female students(p<.01). The study also revealed that male and female students had positive perceptions about the use of biotechnology in the development of beneficial products. However, male students' perceptions were more positive than female students(p<.01). Female students' perceptions were slightly more negative than males and they indicated a measure of caution in the development of beneficial products using biotechnology. Regarding the reliability of biotechnological information acquired from food companies, TV broadcasters, and entertainers, male and female students tended to be highly negative. Students perceived that environmental, religious and ethical issues did not affect the use of biotechnology when asked the effect of these factors on the use of biotechnology. They perceived that food safety and genetic factors of microbes did affect the use of biotechnology. Thus, the study findings suggest that teaching and learning strategies based on the differences of perceptions between male and female students of this study be established and the use of media, development of teaching method and materials be promoted in order to enhance student's performance in environmental education.

• Journal of Microbiology and Biotechnology
• /
• 제28권8호
• /
• pp.1247-1259
• /
• 2018

Raspberries are polyphenol-rich fruits with the potential to reduce the severity of the clinical signs associated with obesity, a phenomenon that may be related to changes in the gut microbiota. The aim of this study was to investigate the effect of raspberry supplementation on the fecal microbiota using an in vivo model of obesity. Obese diabetic db/db mice were used in this study and assigned to two experimental groups (with and without raspberry supplementation). Fecal samples were collected at the end of the supplementation period (8 weeks) and used for bacterial 16S rRNA gene profiling using a MiSeq instrument (Illumina). QIIME 1.8 was used to analyze the 16S data. Raspberry supplementation was associated with an increased abundance of Lachnospiraceae (p = 0.009), a very important group for gut health, and decreased abundances of Lactobacillus, Odoribacter, and the fiber degrader S24-7 family as well as unknown groups of Bacteroidales and Enterobacteriaceae (p < 0.05). These changes were enough to clearly differentiate bacterial communities accordingly to treatment, based on the analysis of UniFrac distance metrics. However, a predictive approach of functional profiles showed no difference between the treatment groups. Fecal metabolomic analysis provided critical information regarding the raspberry-supplemented group, whose relatively higher phytosterol concentrations may be relevant for the host health, considering the proven health benefits of these phytochemicals. Further studies are needed to investigate whether the observed differences in microbial communities (e.g., Lachnospiraceae) or metabolites relate to clinically significant differences that can prompt the use of raspberry extracts to help patients with obesity.

• 한국식품저장유통학회지
• /
• 제5권4호
• /
• pp.346-349
• /
• 1998

개체당 중량과 과육부의 중량은 천일건조한 것이 2.8$\pm$0.03g/ea로 다른 건조방법에 비해 낮았다. 수분함량도 역시 천일건조한 것이 열풍건조를 비롯한 다른 방법에 비해 낮았다. 수분재흡수율은 열풍건조한 것이 다른 건조방법에 비해 높았으며 천일건조한 것이 가장 낮았다. 건조대추의 경도는 천일건조한 것이 8.6$\times$106dyne/$\textrm{cm}^2$으로 다른 건조방법에 비해 월등히 높았다. 부패율은 천일건조대추는 15.3%로 다른 건조방법에 비해 매우 높았고, 갈변도는 천일건조와 천일ㆍ열풍건조를 병행하여 건조한 대추에서 각각 286.5와 196.4%로 다른 건조방법에 비해 월등히 높았다. 탄수화물, 단백질, 지질의 함량은 모든 구에서 유사한 경향을 보였고, 회분의 함량은 천일건조 대추가 다른 건조방법에 비해 높았다. Vitamin C의 함량은 천일건조한 대추가 다른 건조방법에 의해 건조된 대추에 비해 낮았다.

• Journal of Community Nutrition
• /
• 제8권1호
• /
• pp.9-15
• /
• 2006

Health care is one of the most popular reasons for accessing the Internet. Of concern is the amount of information from disreputable sources available on the Internet. A review of websites offering nutrition consulting services suggests sites are controlled by both registered dietitians and non-dietitian professionals marketing themselves as nutrition consultants. The purpose of this study was to investigate structure and content of websites controlled by registered dietitians and nondietitians professionals marketing themselves as nutritionists or nutrition consultants. Internet search queries of 'dietitian', 'dietitian consultant', and 'nutrition consultant' were completed for website selection. Thirty websites controlled by registered dietitians and 10 websites controlled by nutrition consultants were reviewed using an 18-item website evaluation instrument developed for this study. Five evaluators were recruited from the dietetics program at the University of Kentucky. Overall, websites controlled by registered dietitians ranked higher than websites controlled by nutrition consultants in a majority of categories. Sites controlled by registered dietitians ranked statistically higher for the following categories: 'accuracy of information'(p<.0001), 'inclusion of professional resume of owner or primary manager' (p<.05), and 'explanation of the affiliation to externallinks'(p<.05). A majority of sites controlled by both dietitians and nutrition consultants achieved a poor ranking in regards to provision of a legal disclaimer and inclusion of a privacy policy. Prior studies suggest personal privacy is the most important concern for consumers accessing health information on the Internet. Findings from this project will benefit dietitians to assist in development of reputable nutrition related websites.