• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2004년도 총회 및 춘계학술발표회
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• pp.303-306
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• 2004

The objective of this study was to examine the infiltration pattern in a soil developed from granite using tile nonfluorescent and nontoxic food dye, Brilliant Blue FCF (C$_{37}$ $H_{34}$$N_2$Na$_2$$O_{9}$S$_3$) as the dye tracer. A homogeneous matrix flow occurred in the A horizon with weak, medium granular structure and fingering at the interface of finer-textured A horizon and coarser-textured C horizon. Pegmatitic vein originated from the granite and plant root in C horizon induced preferential flow.w.

• Archives of Pharmacal Research
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• 제19권4호
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• pp.269-274
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• 1996

The binding interactions between human serum albumin (HSA) and the edible food dyes amaranth, tartrazine and sunset yellow have been studied. Intrinsic association constants and the free energy changes associated with dye-protein binding at physiological pH for amaranth and tartrazine, and at two different pH values for sunset yellow have been calculated from ultrafiltration data. The temperature dependence $(20-40^{\circ}C)$ of the intrinsic association constants at pH 7.4 for amaranth-HSA and tartrazine-HSA mixtures have been measured, from which a plot of the van't Hoff isochore exhibits a marked change in slope around $30^{\circ}C$ indicating a possible change in protein conformation. The number of dye binding sites on HSA is reported for all the above conditions. HSA-ligand binding enthalpies have been used in conjunction with the N-B transitional binding enthalpy for HSA, to calculate the enthalpy for the N-B transition when ligands are bound with the protein.

• 한국식품위생안전성학회지
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• 제27권2호
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• pp.188-193
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• 2012

본 연구는 SD 계통의 랫드에서 국화 염료인 백색국화, 백색국화 잎과 줄기, 황색국화의 급성경구독성 및 피부자극성을 평가하기 위해서 시행하였다. 수컷 랫드에 처치한 물질은 백색국화, 백색국화 잎과 줄기, 황색국화를 각각 1 ml/kg, 2 ml/kg를 D.W에 용해시켜 단회 경구 투여하였으며, 수컷 SD rats에 투여 후 14일 동안 관찰하였으며, 피부자극성 시험은 피부의 찰과 및 비찰과부의 대조부, 처치부에 국화 염료를 처치하여 3일 동안 관찰하였다. 단회 경구 투여 및 피부 자극성 시험 후 국화 염료의 안전성을 확인하기 위해 체중과 사료 섭취량, 임상증상, 안과학적 검사, 사망률, 피부자극성을 관찰하고, 랫드 피부에서의 병리조직학적 변화를 관찰하였다. 체중, 사료섭취량, 병리조직학적 변화가 대조군과 비교시 유의적인 변화가 나타나지 않았다. 또한 피부자극성을 확인 한 결과 1차 자극 지수가 0으로 비자극성 물질로 분류되었다. 따라서 본 연구를 통하여 국화에서 추출한 염료가 급성 경구독성 시 안전한 물질이며, 피부 자극성을 야기 하지않는 물질로 평가된다.

• 한국염색가공학회지
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• 제30권3호
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• pp.208-215
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• 2018

The objective was to investigate the efficacy of Metasequoia(Metasequoia glyptostroboides) cone colorants as a new functional dye for wool fiber. Effects of dyeing conditions and mordanting on dye uptake, color change, and colorfastness were investigated for wool fiber. Compared with cotton and silk fibers, wool fiber showed better affinity for the Metasequoia cone colorants. Fe and Cu mordants improved dye uptake, and mordanting did not change the color of dyed fabric with YR Munsell color. Colorfastness to rubbing and washing was as good as grade 4, whereas lightfastness of the dyed fabrics was above grade 3. Antimicrobial activity of the colorants was very high 99.3% of reduction rate toward S. aureus. It was confirmed that Metasequoia cone colorants can be utilized as a new functional natural dye for wool fiber. In addition, considering its high antimicrobial functionality it could be applied to cosmetics and food.

• 한국생활과학회지
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• 제16권1호
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• pp.205-218
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• 2007

The purpose of this study was to provide informations on what middle school students know and think about food additives, and their purchase behavior of processed foods containing food additives. The data were gathered by questionaries from 360 students attending in the 3 randomly selected middle schools around Gyeonggi Province. The valid 340 out of 360 questionaries were analysed by SPSS/WIN 12.0. As a result, the middle school students showed low interest in 4 kinds of representative food additives such as synthetic seasoning, artificial sweetener, artificial dye, and synthetic preservative. They thought that the food additives is necessary but not improve the quality of food, and may have harmful effects on health. The students who has mother of university graduate, and taught by text book A showed the highest interest. Most students rarely checked the indication of food additives on the processed food package, and they hardly put any effort to avoid the processed foods containing food additives. According to the results of this study, the students were not aware and did not know about the food additives enough, and needs to be educated more and in detail in home economics class.

• 한국식품영양과학회지
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• 제27권6호
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• pp.1160-1165
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• 1998

When total cellular DNA was isolated from Porphyra tenera by ultracentrifugation on Hoechst dye/CsCl gradients method, plasmid like DNA's were concentrated at the upper band which were characterized with a A＋T rich organelle DNA's in the CsCl gradients. Based on their electrophoretic migration in different concentration of agarose gel, buffer system, and electric power etc. and the results of restriction digestion, the plasmid like DNA's were concluded to have circular conformation. This is the first report of putative circular plasmid DNA from the P. tenera, which is a autonomously replicating plasmid existing with a high copy number plasmid in the cell. The minimum size of this plasmid estimated by restriction endonuclease digestion was appeared to be 2.5kb in size.

• Journal of Photoscience
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• 제9권2호
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• pp.409-411
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• 2002

In order to prevent side reactions due to free radical formation occurring in the reactions of singlet oxygen generated in solution phse, it was required that singlet oxygen is generated in gas phase from an apparatus of a solid-gas system. We have accomplished to construct an apparatus generating singlet oxygen in solid-gas system, which is composed of a flexible optical fibre tube connected by a dye-sensitizer probe containing rose bengal dye on polymer or inorganic material. Through the optical fibre tube visible light from a laser and an oxygen stream are passed into the sensitizer probe where singlet oxygen is generated. The determination of singlet oxygen was carried out by two methods. One involves the detection of the luminescence of singlet oxygen at 1268 mn and the other involves the chemiluminescence reaction of a dihydroisobenzofuran with singlet oxygen emitting luminescence at 456 nm.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권4호
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• pp.309-312
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• 2004

Aspergillus sojae B-l0 was immobilized and used to treat model dye compounds. The model wastewater, containing 10 ppm of azo dyes such as Amaranth, Sudan III, and Congo Red, was treated with cells attached to a rotating disc contactor (RDC). Amaranth was decolorized more easily than were Sudan III and Congo Red. Decolorization of Amaranth began within a day, and the dye was completely decolorized within 5 days of incubation. Both Sudan III and Congo Red were almost completely decolorized after 5 days of incubation. Semicontinuous decolorization of azo by reusing attached mycelia resulted in almost complete decolorization in 20 days. This experiment indicated that decolorization was successfully conducted by removing azo dyes with Aspergillus sojae B-10.

• 한국응용과학기술학회지
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• 제16권3호
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• pp.199-203
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• 1999

The symmetric benzoxazolo carbocyanine is of industrial importance as green-sensitizing dye in the spectral sensitization of emulsion microcrystals in positive paper and negative film-making. The stability on the solvents of benzoxazolo carbocyanine dye was measured by UV-Vis spectrophotometer, and then all of solvents were stabilized sensitizer. The maximum absorption peak range in methanol, acetonitrile, acetone, DMF, dichloromethane, chloroform solvents was $501nm{\sim}511nm$. But it was identified that only methanol can be used to photographic emulsion. The photographic characteristics have contrast of 2.8, speed of 50-55$(lux{\cdot}sec)^{-1}$, fog of 0.07-0.08, respectively.

• Journal of Microbiology and Biotechnology
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• 제12권4호
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• pp.544-552
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• 2002

Several microorganisms from rat and human feces and lumen fluid of cows were screened for their ability to decolorize the synthetic dyes. Consequently, a novel dye-degrading strain AB&J was isolated. Taxonomic identification including 165 rDNA sequencing and phylogenetic analysis indicated that the isolate had 99.9% homology in its 165 rDNA base sequence with Clostridium perfringens. After 27 h Incubation with the strain, brilliant blue R, bromophenol blue, crystal violet, malachite green, methyl green, and methyl orange were decolorized by about 69.3%, 97.7%, 96.3%, 97.9%, 75.1%, and 97.2%, respectively. The triphenlmethane dye, bromophenol blue, was decolorized extensively by growing Clostridium perfringens AB&J cells in liquid cultures under anaerobic condition, although their growth was strongly inhibited in the initial stage of incubation. This group of dyes is toxic, depending on the concentration used. The dye was significantly decolorized at a relatively lower concentration of below 50 $\mu g \;ml^{-1}$, however, the growth of the cells was mostly suppressed at a dye concentration of 100 $\mu g \;ml^{-1}$. The decolorization activity in cell-free extracts was much higher in cytoplasm than in periplasm and cytoplasmic membrane. Therefore, the enzyme related uptake of bromophenol blue seemed to be localized in cytoplasm. The optimal pH and temperature of bromophenol blue uptake fur decolorization activities were 7.0 and 4$0^{\circ}C$, respectively.