• Biomolecules & Therapeutics
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• 제22권6호
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• pp.558-562
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• 2014

Tramadol is an opioid analgesic agent that has been the subject of a series of case reports suggesting potential for misuse or abuse. However, it is not a controlled substance and is not generally considered addictive in Korea. In this study, we examined the dependence potential and abuse liability of tramadol as well as its effect on the dopaminergic and serotonergic systems in rodents. In animal behavioral tests, tramadol did not show any positive effects on the experimental animals in climbing, jumping, and head twitch tests. However, in the conditioned place preference and self-administration tests, the experimental animals showed significant positive responses. Taken together, tramadol affected the neurological systems related to abuse liability and has the potential to lead psychological dependence.

• Asian-Australasian Journal of Animal Sciences
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• 제27권2호
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• pp.284-289
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• 2014

The experiment was conducted to evaluate the ingestive behavior of lambs confined in individual and group stalls. We used thirty-four lambs in their growing phase, aged an average of three months, with mean initial live weight of $17.8{\pm}5.2$ kg. They were allotted in a completely randomized design with 24 animals kept in individual stalls and 10 animals confined as a group. The experiment lasted for a total of 74 days, and the first 14 days were dedicated to the animals' adaption to the management, facilities and diets. The data collection period lasted 60 days, divided into three 20-d periods for the behavior evaluation. The animals were subjected to five days of visual observation during the experiment period, by the quantification of 24 h a day, with evaluations on the 15th day of each period and an interim evaluation consisting of two consecutive days on the 30th and 31st day of the experiment. The animals confined as a group consumed less (p<0.05) fiber. However, the animals confined individually spent less (p<0.05) time on feeding, rumination and chewing activities and longer in idleness. Therefore, the lower capacity of lambs confined in groups to select their food negatively affects their feeding behavior.

• Food Science and Biotechnology
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• 제17권1호
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• pp.191-194
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• 2008

The antagonistic activity of probiotic strains (Bifidobacterium animalis BB-12, Bifidobacterium bifidum A, Bifidobacterium longum B6, Lactobacillus acidophilus ADH, Lactobacillus paracasei ATCC 25598, and Lactobacillus rhamnosus GG) against nalidixic acid resistant ($NA^R$) Escherichia coli O157:H7 MF1847, E. coli O157:H7 H2439, E. coli O157:H7 ATCC 43894, and E. coli O157:H7 C7927 was investigated using the agar-overlay, well diffusion, and broth culture tests. L. paracasei ATCC 25598 was the most effective probiotic strain in terms of in vitro antagonistic activity against $NA^R$ E. coli O157:H7, followed by L. rhamnosus GG, B. longum B6, and L. acidophilus ADH. The use of selected probiotic strains could be an effective pre-harvest intervention strategy to reduce the risk of $NA^R$ E. coli O157:H7 by maintaining a balanced microflora in animals and might provide many potential benefits in lieu of using antimicrobials.

• Asian-Australasian Journal of Animal Sciences
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• 제14권3호
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• pp.423-431
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• 2001

Animals are one of the important memberships of the food chain. The low-efficiency rule of nutrient transfer from one member to the next in the food chain determines the low efficiency of animal agriculture for human food. On the average, about 20% feed proteins and 15% feed energy can be converted into edible nutrients for humans. The rest proportion of feed nutrients is exposed to the environment. Environmental pollution, therefore, is inevitable as animal agriculture grows intensively and extensively. The over-loading of the environment by nutrients such as nitrogen, phosphorus from animal manure results in soil and water spoilage. The emission of gases like $CH_2$, $CO_2$, $SO_2$, NO, $NO_2$ by animals are one of the contributors for the acidification of the environment and global warming. The inefficient utilization of natural resources and the probable unsafety of animal products to human health are also a critical environmental issue. Improving the conversion efficiency of nutrients in the food chain is the fundamental strategy for solving environmental issues. Specifically in animal agriculture, the strategy includes the improvements of animal genotypes, nutritional and feeding management, animal health, housing systems and waste disposal programs. Animal nutrition science plays a unique and irreplaceable role in the control of nutrient input and output in either products or wastes. Several nutritional methods are proved to be effective in alleviating environmental pollution. A lot of nutritional issues, however, remain to be further researched for the science of animal nutrition to be a strong helper for sustainability of animal agriculture.

• 한국축산식품학회지
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• 제41권5호
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• pp.883-893
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• 2021

Clostridium difficile present in feces of food animals may contaminate their meats and act as a potential source of C. difficile infection (CDI) to humans. C. difficile resistance to antibiotics, its production of toxins and spores play major roles in the pathogenesis of CDI. This is the first study to evaluate C. difficile prevalence in retail raw animal meats, its antibiotics susceptibilities and toxigenic activities in Al-Jouf, Saudi Arabia. Totally, 240 meat samples were tested. C. difficile was identified by standard microbiological and biochemical methods. Vitek-2 compact system confirmed C. difficile isolates were 15/240 (6.3%). Toxins A/B were not detected by Xpect C. difficile toxin A/B tests. Although all isolates were susceptible to vancomycin and metronidazole, variable degrees of reduced susceptibilities to moxifloxacin, clindamycin or tetracycline antibiotics were detected by Epsilon tests. C. difficile strains with reduced susceptibility to antibiotics should be investigated. Variability between the worldwide reported C. difficile contamination levels could be due to absence of a gold standard procedure for its isolation. Establishment of a unified testing algorithm for C. difficile detection in food products is definitely essential to evaluate the inter-regional variation in its prevalence on national and international levels. Proper use of antimicrobials during animal husbandry is crucial to control the selective drug pressure on C. difficile strains associated with food animals. Investigating the protective or pathogenic potential of non-toxigenic C. difficile strains and the possibility of gene transfer from certain toxigenic/ antibiotics-resistant to non-toxigenic/antibiotics-sensitive strains, respectively, should be worthy of attention.

• Journal of Forest and Environmental Science
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• 제24권2호
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• pp.89-97
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• 2008

Based on the former researches, this article studied the influence of cone harvesting of Korean pine on the regeneration of Korean pine trees and the life of animals in Mt. Changbai Nature Reserve. When the cone matures, scales of the cone do not open so the seeds can not be released automatically. And the seeds, if left inside, are hard to germinate and can not grow further into seedlings. The seeds of Korean pine have deep dormancy characteristics. Natural regeneration of Korean pine is very poor under mother trees. Hoarding behavior of dispersing animals not only helps animals for food shortage period but also contributes to the dispersion of seeds of Korean pine. Among those hoarding animals, squirrel and Eurasian nutcrackers are found to be the most important dispersing agents for the seeds of Korean pine. After cone harvesting, the number of those dispersers reduced a lot since the seeds of Korean pine are very important food for them. Seed quantity of Korean pine on surface layer became very few and most of them only showed single distribution. Most of the seeds were buried under litter layer and showed a single or 2-4 seeds/cluster distribution. The case of more than 4 seeds in one cluster was few. The seed quantity of Korean pine forest on steep slopes of the research area was only 0.3% of the seed quantity in 1980 for the same forest type. If seed source of Korean pine are not protected, Korean pine forest in Mt. Changbai Nature Reserve would not maintain present feature in the future.

• Biomolecules & Therapeutics
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• 제21권4호
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• pp.307-312
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• 2013

Quetiapine is an atypical or second-generation antipsychotic agent and has been a subject of a series of case report and suggested to have the potential for misuse or abuse. However, it is not a controlled substance and is not generally considered addictive. In this study, we examined quetiapine's dependence potential and abuse liability through animal behavioral tests using rodents to study the mechanism of quetiapine. Molecular biology techniques were also used to find out the action mechanisms of the drug. In the animal behavioral tests, quetiapine did not show any positive effect on the experimental animals in the climbing, jumping, and conditioned place preference tests. However, in the head twitch and self-administration tests, the experimental animals showed significant positive responses. In addition, the action mechanism of quetiapine was found being related to dopamine and serotonin release. These results demonstrate that quetiapine affects the neurological systems related to abuse liability and has the potential to lead psychological dependence, as well.

• Animal Bioscience
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• 제34권4호
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• pp.662-669
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• 2021

Objective: Effects of linseed oil (LO) supplementation on the fat content and fatty acid profile of breast meat, and the expression of three genes in the liver, breast muscle and fat tissues of commercial 154-day-old hybrid male turkeys were investigated. Methods: The animals in the control group were fed a commercially available feed and received no LO supplementation (n = 70), whereas animals in the LO group (n = 70) were fed the same basic diet supplemented with LO (day 15 to 21, 0.5%; day 22 to 112, 1%). The effect of dietary LO supplementation on fatty acid composition of breast muscle was examined by gas chromatography, and the expression of fatty acid desaturase 2 (FADS2), peroxisome proliferator activated receptor gamma (PPARγ), and insulin-like growth factor 1 (IGF1) genes was analysed by means of quantitative reverse transcription polymerase chain reaction. Results: The LO supplementation affected the fatty acid composition of breast muscle. Hepatic FADS2 levels were considerably lower (p<0.001), while adipose tissue expression was higher (p<0.05) in the control compared to the LO group. The PPARγ expression was lower (p<0.05), whereas IGF1 was higher (p<0.05) in the fat of control animals. There were no significant (p>0.05) differences in FADS2, PPARγ, and IGF1 gene expressions of breast muscle; however, omega-6/omega-3 ratio of breast muscle substantially decreased (p<0.001) in the LO group compared to control. Conclusion: Fatty acid composition of breast meat was positively influenced by LO supplementation without deterioration of fattening parameters. Remarkably, increased FADS2 expression in the liver of LO supplemented animals was associated with a significantly decreased omega-6/omega-3 ratio, providing a potentially healthier meat product for human consumption. Increased PPARγ expression in fat tissue of the LO group was not associated with fat content of muscle, whereas a decreased IGF1 expression in fat tissue was associated with a trend of decreasing fat content in muscle of the experimental LO group.

• 문화기술의 융합
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• 제7권4호
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• pp.833-844
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• 2021

코로나 시대에 반려동물은 우리에게 많은 위안을 주고, 가족처럼 반려동물을 여기는 펫팸족이 등장하였다. 그리고 젊은 소비자는 자신에게 소비할 금액을 줄여서 반려동물에게 소비하는 패턴을 보이는 펫푸어도 볼 수 있다. 하지만 이러한 시대적인 변화에도 불구하고 펫푸드를 포함한 반려동물 용품 및 화장품은 여전히 수입 의존도가 높고, 국내의 반려동물 화장품 및 용품에 대한 기준이 설정되어 있지 않아 화장품 안전기준 등에 관한 규정에만 의존하고 있는 실정이다. 또한 국내 반려동물 용품 및 화장품에 관한 용어도 아직 정립되지 않은 상태이다. 현재 국내 법률은 반려동물과 관련한 동물보호법 만이 존재하지만 일본에서는 반려동물 사료안전법이 2009년 6월부터 시행되었고, 미국은 연방식품, 의약품, 화장품 관리법에서 반려동물 화장품 및 용품을 담당하고, 동물보호법이 강력한 독일은 식품 및 사료에 대한 다양한 국가 차원의 법규가 있다. 그리고 식품 안전국 웹 사이트에서도 국가 규정을 정밀하게 확인 가능하다. 따라서 본 연구는 국외의 빠르게 성장하고 안정적인 반려동물 산업처럼 국내에서도 반려 동물 산업을 관리하고 개발을 뒷받침 해주는 국가 정책과 환경 등이 반려동물 용품 및 화장품 그 외 다양한 반려동물 관련 산업을 해외시장으로 수출하는데 기초자료로 반드시 필요할 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제31권7호
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• pp.1062-1071
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• 2018

The misuse of anabolic hormones or illegal drugs is a ubiquitous problem in animal husbandry and in food safety. The ban on growth promotants in food producing animals in the European Union is well controlled. However, application regimens that are difficult to detect persist, including newly designed anabolic drugs and complex hormone cocktails. Therefore identification of molecular endogenous biomarkers which are based on the physiological response after the illicit treatment has become a focus of detection methods. The analysis of the 'transcriptome' has been shown to have promise to discover the misuse of anabolic drugs, by indirect detection of their pharmacological action in organs or selected tissues. Various studies have measured gene expression changes after illegal drug or hormone application. So-called transcriptomic biomarkers were quantified at the mRNA and/or microRNA level by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) technology or by more modern 'omics' and high throughput technologies including RNA-sequencing (RNA-Seq). With the addition of advanced bioinformatical approaches such as hierarchical clustering analysis or dynamic principal components analysis, a valid 'biomarker signature' can be established to discriminate between treated and untreated individuals. It has been shown in numerous animal and cell culture studies, that identification of treated animals is possible via our transcriptional biomarker approach. The high throughput sequencing approach is also capable of discovering new biomarker candidates and, in combination with quantitative RT-qPCR, validation and confirmation of biomarkers has been possible. These results from animal production and food safety studies demonstrate that analysis of the transcriptome has high potential as a new screening method using transcriptional 'biomarker signatures' based on the physiological response triggered by illegal substances.