• Journal of Microbiology and Biotechnology
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• 제28권1호
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• pp.87-94
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• 2018

This study investigated the feasibility of the lytic, tailed Bacillus cereus-specific phage for use in a ferromagnetoelastic (FME) biosensor as a novel recognition element. The phage was immobilized at various concentrations through either direct adsorption or a combination of 11-mercapto-1-undecanoic acid (11-MUA) and [N-(3-dimethylaminopropyl)-N'-carbodiimide hydrochloride and N-hydroxysuccinimide (EDC/NHS)]. The effects of time and temperature on its lytic properties were investigated through the exposure of B. cereus (4 and 8 logCFU/ml) to the phage (8 logPFU/ml) for various incubation periods at $22^{\circ}C$ and at various temperatures for 30 and 60 min. As the phage concentration increased, both immobilization methods also significantly increased the phage density (p < 0.05). SEM images confirmed that the phage density on the FME platform corresponded to the increased phage concentration. As the combination of 11-MUA and EDC/NHS enhanced the phage density and orientation by up to 4.3-fold, it was selected for use. When various incubation was conducted, no significant differences were observed in the survival rate of B. cereus within 30 min, which was in contrast to the significant decreases observed at 45 and 60 min (p < 0.05). In addition, temperature exerted no significant effects on the survival rate across the entire temperature range. This study demonstrated the feasibility of the lytic, tailed B. cereus-specific phage as a novel recognition element for use in an FME biosensor. Thus, the phage could be placed on the surface of foods for at least 30 min without any significant loss of B. cereus, as a result of the inherent lytic activity of the B. cereus-specific phage as a novel recognition element.

• 기본간호학회지
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• 제9권3호
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• pp.349-359
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• 2002

Purpose: The purpose of this study was two-fold : to describe the level of recognition of nosocomial respiratory infections by ICU nurses and to compare self evaluation and objective evaluation of nosocomial respiratory infection control practices by ICU nurses. Method: Data were collected from 78 nurses in intensive care units in one university affiliated hospital in Kyung Ki Province. Data were collected from March 4 to March 18, 2002. The recognition and self evaluation data were collected through a self report questionnaire and an objective evaluation which was done by observing the actual behavior of the nurses. Result: The results of this study showed that there was a significant positive relationship between recognition scores and self evaluation scores. However, there was no significant relationship between level of recognition and objective evaluation, nor between self evaluation and objective evaluation of infection control Practices by ICU nurses. Conclusion: ICU nurses In this study gave higher scores on self evaluation of their practice than were shown in the observation evaluation of actual practice. Moreover, in the objective evaluation the nurses had the lowest score on the item, 'washing hands before taking care of patients'. The results indicate that it is necessary to develop a standardized practice manual on nosocomial respiratory infection control in the ICU. Also, it is necessary to have a program to transfer knowledge into actual practice.

• 정보처리학회논문지:소프트웨어 및 데이터공학
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• 제5권9호
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• pp.419-424
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• 2016

특허 정보검색에서는 검색 정확도를 높이거나 유사 특허들을 검색하기 위한 목적으로 청구항 등 특허 기술 문서의 내용을 대표하는 개체명 인식이 필요하다. 본 연구에서는 특허 개체명을 자동으로 인식하기 위하여 기계 학습 기법에서 태깅 문제 해결에 매우 우수한 성능을 보이는 조건부 랜덤 필드 기법을 이용하는 특허 개체명 인식 방법을 제안하였다. 개체명 태깅이 되어 있는 특허 문서 말뭉치에서 66만 어절을 학습용 데이터로 사용하여 특허 개체명 시스템을 구축하고, 7만 어절을 평가용 데이터로 사용하여 성능 평가를 하였다. 실험 결과에 의하면 개체명 인식 정확도는 93.6%이고, 개체명 인식 성능을 수작업 태깅 결과와 비교하여 일치도를 평가했을 때 카파 계수는 0.67로 나타났다. 이 카파 계수값은 두 사람의 수작업 태깅 결과에 대한 카파 계수 0.6 보다 높은 것으로 특허 개체명 인식 시스템이 수작업 태깅을 대신하여 실용적으로 활용될 수 있음을 확인하였다.

• 한국자기공명학회논문지
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• 제11권1호
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• pp.10-23
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• 2007

A new disintegrin protein named saxatilin was purified from Korean snake venom (Gloydius saxatilis). Saxatilin is a 73 residue small ploypeptide, which has a primary recognition motif in extracellular matrix, Arg-Gly-Asp (RGD) sequence. Data from inhibition activity assay for the ${\alpha}_v{\beta}_3$ integrin showed that saxatilin showed about 5000-fold higher activity than those of RGD peptides, suggesting that RGD sequence may not be sufficient to induce full cellular function of this site. The solution structures calculated from NMR data were well converged for backbone atoms except RGD loop. The structure revealed that most of tight turns are stabilized by medium range NOE contacts and the RGD motif is located far from the rigid core of the C-terminal domain. The three-dimensional fold and biological function of saxatilin are discussed with those of salmosin, which is a disintegrin protein derived from Agkistrodon halys brevicaudus.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2016년도 춘계학술대회
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• pp.285-286
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• 2016

해상교통관제센터에 의해 실시간으로 수집되는 선박의 항해 데이터를 바탕으로 선박 항적 패턴 인식을 수행하고 이를 바탕으로 항적 모델을 추출하여 사전에 선위를 예측하는 기법을 제안한다. 항적 데이터의 처리와 가공, 항적 모델링을 위하여 Support Vector Regression 알고리즘이 사용되었으며, 적정 파라미터 선정을 위하여 k-fold cross validation과 grid search가 사용되었다. 제안된 항적 데이터 모델링 기법을 통하여 사전에 선박의 선위를 예측하여 해상교통과제사의 의사결정을 지원하고자 한다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제10권2호
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• pp.769-786
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• 2016

Object recognition and object location have always drawn much interest. Also, recently various computational models have been designed. One of the big issues in this domain is the lack of an appropriate model for extracting important part of the picture and estimating the object place in the same environments that caused low accuracy. To solve this problem, a new Salient Based Bag of Visual Word (SBBoVW) model for object recognition and object location estimation is presented. Contributions lied in the present study are two-fold. One is to introduce a new approach, which is a Salient Based Bag of Visual Word model (SBBoVW) to recognize difficult objects that have had low accuracy in previous methods. This method integrates SIFT features of the original and salient parts of pictures and fuses them together to generate better codebooks using bag of visual word method. The second contribution is to introduce a new algorithm for finding object place based on the salient map automatically. The performance evaluation on several data sets proves that the new approach outperforms other state-of-the-arts.

• Current Research on Agriculture and Life Sciences
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• 제11권
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• pp.121-132
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• 1993

대두(大豆)-근류균(根瘤菌)의 공생(共生)에서 숙주식물(宿主植物)의 Root Exudate에 대한 근류균(根瘤菌)의 화학주성(化學走性)이 근류형성(根瘤形性)(숙주식물(宿主植物))에 미치는 영향(影響)을 밝히기 위해 연구(硏究)한 결과(結果)는 다음과 같다. 대두(大豆) Seed Lectin은 품종간(品種間) 차이(差異)가 없었으며 분자량(分子量) 120,000정도(程度)의 4개(個)의 동일(同一)한 subunit를 가지는 물질(物質)이며, Pea Seed Lectin은 분자량(分子量) 44,000정도(程度)이며 15,000과 7,000정도(程度)의 두 종류(種類)의 subunit를 가지는 물질(物質)이며 대두(大豆) lectin은 표준 lectin 항체와 침강선을 형성하였다. 그러나 완두의 lectin은 표준 lectin과 항원-항체반응이 없었으므로 두 lectin은 동일 항원이 되지 않아 서로 다른 물질이었다. 팔달의 Crude Root Exudate에 대(對)한 근류균(根瘤菌)의 화학주성(化學走性) 비(比)는 KCTC 2422는 각각(各各) 3.5이고 LPN-100은 1.4이며 LCR-101은 각각(各各) 1.4이었다. 뿌리분비물의 각 fraction에 대한 근류균의 화학주성은 중성분획이 가장 높고 양성분획이 그다음이며 음성분획이 가장 낮은 화학주성을 나타내어 각물질에 대한 근류균의 친화도에 차이가 있었다. 근류형성(根瘤形性)의 정도(程度)는 KCTC 2422의 경우(境遇)는 접종(接種)후 7日경부터 Nitrogenase Activity가 나타났으며 LCR-101는 15일 이후(以後)에 Nitrogenase Activity를 나타내었다. LPN-100은 Nitrogenase Activity가 전혀 나타나지 않아 근류균(根瘤菌)의 화학주성(化學走性)이 숙주인식과정(宿主認識過程)에 영향(影響)을 미쳤음을 확인(確認)할 수 있었다.

• Architectural research
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• 제19권3호
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• pp.53-61
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• 2017

This study aims to understand and analyze the social recognition and debate shown during the progress of the Asian Culture Complex (ACC) public project as well as the environmental evaluation performed by provincial community members. A 30-question survey based on a Likert scale was conducted with 150 experts. The study showed, first, that despite controversies over landmarks and historical locations, the quality of the architect's first draft was considered fairly high. However, vibrant landmarks were greatly desired. Furthermore, respondents reported conflicting recognitions of the same concept, which were defined differently depending on the terms and concepts used by the architect. Second, historical locations were considered important. The architects' final design was modified based on quality and in response to social pressure, resulting in the partial retention of the provincial government annex building. Although the first draft was considered to be of high quality, the modified plan was considered even better than the original design, revealing the 2-fold characteristics of the identical phenomenon in the environmental evaluation. Third, many groups held opinions that were balanced between the architects' originality and the value of community-based recognition; hence, the social inclination and evaluation of the project differed across the public.

• 한국인터넷방송통신학회논문지
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• 제14권3호
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• pp.139-146
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• 2014

본 논문에서는 얼굴 영상으로부터 자동으로 사람의 감정을 인식하는 효과적인 방법을 제안한다. 얼굴 표정으로부터 감정을 파악하기 위해서는 카메라로부터 얼굴영상을 입력받고, ASM (active shape model)을 이용하여 얼굴의 영역 및 얼굴의 주요 특징점을 추출한다. 추출한 특징점으로부터 각 장면별로 49차의 크기 및 변이에 강인한 특징벡터를 추출한 후, 통계기반 패턴분류 방법을 사용하여 얼굴표정을 인식하였다. 사용된 패턴분류기는 Naive Bayes, 다중계층 신경회로망(MLP; multi-layer perceptron), 그리고 SVM (support vector machine)이며, 이중 SVM을 이용하였을 때 가장 높은 최종 성능을 얻을 수 있었으며, 6개의 감정분류에서 50.8%, 3개의 감정분류에서 78.0%의 인식결과를 보였다.

• 생명과학회지
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• 제17권2호통권82호
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• pp.179-184
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• 2007

단백질 티로신 kinase인 PTK6는 대부분의 유방암에서 과발현되며, 암세포의 증식만을 촉진하는데 역할을 한다. 이 연구에서 PTK6의 활성도메인을 초파리의 peptidoglycan recognition protein (PGRP) -LB 단백질을 퓨전파트너로 사용하여 바큘로바이러스 시스템을에서 과발현하는데 성공하였다. 우리는 PGRP-LB가 바큘로바이러스 시스템에서 잠재적으로 퓨전 단백질로 사용될 수 있는 가능성을 처음으로 발견하였다. 정제된 PTK6단백질은 기존의 박테리아에서 발현된 단백질보다 1.5배 높은 활성을 지녔다. 이 단백질은 PTK6의 분자기전 및 그것의 저해제 개발에 필수적인 결정 구조를 규명하는데 사용될 것이다.